Hydrolysis of nucleic acids in single-cell protein concentrates using immobilized benzonase.

Abstract:

:Hydrolysis of nucleic acids for single-cell protein concentrates has been carried out in one step using immobilized benzonase on corn cob. The immobilization is carried out by tosylation of primary alcohols of cellulose of corn cob. The immobilized benzonase is more stable vs pH changes than native benzonase, but the same optimum values of [Mg(II)] and temperature are obtained. The DNase activity is greater than the RNase activity. The percentage of DNA is reduced to 3-6% and that of RNA to 50%. The protein loss is negligible (1%). The enzymatic activity per weight unit of enzyme is greater in the case of benzonase that in reported data for other nucleases insolubilized on corn cob by the same procedure.

authors

Moreno JM,Sanchez-Montero JM,Ballesteros A,Sinisterra JV

doi

10.1007/BF02922124

subject

Has Abstract

pub_date

1991-10-01 00:00:00

pages

43-51

issue

1

eissn

0273-2289

issn

1559-0291

journal_volume

31

pub_type

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