Abstract：
:The rhg gene encoding a rhamnogalacturonase was isolated from the novel strain A1 of Aspergillus niger. It consists of an ORF of 1.505 kb encoding a putative protein of 446 amino acids with a predicted molecular mass of 47 kDa, belonging to the family 28 of glycosyl hydrolases. The nature and position of amino acids comprising the active site as well as the three-dimensional structure were well conserved between the A. niger CTM10548 and fungal rhamnogalacturonases. The coding region of the rhg gene is interrupted by three short introns of 56 (introns 1 and 3) and 52 (intron 2) bp in length. The comparison of the peptide sequence with A. niger rhg sequences revealed that the A1 rhg should be an endo-rhamnogalacturonases, more homologous to rhg A than rhg B A. niger known enzymes. The comparison of rhg nucleotide sequence from A. niger A1 with rhg A from A. niger shows several base changes. Most of these changes (59 %) are located at the third base of codons suggesting maintaining the same enzyme function. We used the rhamnogalacturonase A from Aspergillus aculeatus as a template to build a structural model of rhg A1 that adopted a right-handed parallel β-helix.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Damak N,Abdeljalil S,Taeib NH,Gargouri Adoi
10.1007/s12010-015-1720-1subject
Has Abstractpub_date
2015-08-01 00:00:00pages
2314-27issue
8eissn
0273-2289issn
1559-0291journal_volume
176pub_type
杂志文章abstract：:Culture fluorescence measurement technique has the potential for on-line characterization of metabolic status of fermentation processes. Many fluorophores present inside the living cells such as NADH + H+, tryptophan, pyridoxine, and riboflavin fluoresce at specific excitation and emission wavelength combinations. Sin...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-008-8395-9
更新日期：2009-05-01 00:00:00
abstract：:Paecilomyces variotii isolated from a broad range of habitats drives the diversification of new high-value-added secondary metabolites that could potentially play an important role in human and animal health. These metabolites include the anhydride metabolite of the nonadride family, as well as the following compounds...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/s12010-015-1783-z
更新日期：2015-10-01 00:00:00
abstract：:The Institute of Gas Technology (IGT) conducted a pilot-scale study at a former manufactured gas plant (MGP) site in New Jersey. The objective of the study was to determine the effectiveness of an innovative chemical/biological treatment process (MGP-REM process) to remediate soils contaminated with polynuclear aromat...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02920473
更新日期：1997-04-01 00:00:00
abstract：:Events from the past growth conditions influence the course of physiology, and it gets reflected in the present cell behaviour. During this process, cells acquire a metabolic option which carries signature of the past, and it dictates the performance in the present situation. Study uses Escherichia coli as a sample or...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-1167-9
更新日期：2014-11-01 00:00:00
abstract：:Drinking water distribution systems are composed of various pipe materials and may harbor biofilms even in the continuous presence of disinfectants. Biofilms formation on five pipe materials (copper (Cu), polyethylene (PE), stainless steel (STS), cast iron (CI), and concrete-coated polycarbonate (CP)) within drinking ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-0935-x
更新日期：2014-07-01 00:00:00
abstract：:Fluorescent proteins from marine organisms represent potential candidates for biosensor development. In this paper, we described the isolation of a native green fluorescent protein from Anemonia sulcata and the cloning and purification of its equivalent as a recombinant protein in Escherichia coli. Furthermore, the sp...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0669-1
更新日期：2014-02-01 00:00:00
abstract：:The objective of present investigation was to study the effect of gut microbiota alteration by oral administration of targeted delivery of pH sensitive cefdinir microspheres to high-fructose-fed (HFD) rats. Rats were fed with a high-fructose diet with or without cefdinir microsphere administration for 30 days. The fec...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-0772-y
更新日期：2014-04-01 00:00:00
abstract：:In the fermentation of lignocellulose hydrolysates to ethanol, two major problems are encountered: the fermentation of the pentose sugar xylose, and the presence of microbial inhibitors. Xylose can be directly fermented with yeasts, such as Pachysolen tannophilus, Candida shehatae, and Pichia stipis, or by isomerizati...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02922595
更新日期：1991-04-01 00:00:00
abstract：:The recovery of alpha-amylase from the crude enzyme preparation by the reversed micellar liquid-liquid extraction was investigated. The reversed micellar solution was formed by dissolving a cationic surfactant Aliquat 336 in six different alkanes (cyclohexane, n-hexane, isooctane, n-octane, n-decane, and n-dodecane) r...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02787989
更新日期：1997-02-01 00:00:00
abstract：:The thermostable esterase from the thermophilic bacterium Clostridium thermocellum DSM 1313 was expressed in Escherichia coli and purified by Ni(2+) affinity chromatography. Its molecular weight was approximately 35 kDa according to 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Th...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1824-7
更新日期：2015-12-01 00:00:00
abstract：:This research describes the development of a multi-analyte lateral-flow immunoassay intended for the simultaneous detection of three anti-protozoan drugs (coccidiostats). These drugs, namely, halofuginone, toltrazuril and diclazuril, are used in the treatment of Eimeria spp. infections in cattle, pigs, chickens and tu...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1582-6
更新日期：2015-05-01 00:00:00
abstract：:In this work, we engineered the α/β fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the α/β fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high ac...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-010-9027-8
更新日期：2011-01-01 00:00:00
abstract：:A new process for enzymatic synthesis of biodiesel at high water content (10-20%) with 96% conversion by lipase from Candida sp. 99-125 was studied. The lipase, a no-position-specific lipase, was immobilized by a cheap cotton membrane and the membrane-immobilized lipase could be used at least six times with high conve...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:128:2:109
更新日期：2006-02-01 00:00:00
abstract：:A comparative analysis of the structural and functional aspects along with equilibrium unfolding of two homologous cutinases, Cut1 and Cut2, from Thermobifida fusca was carried out. The CD and fluorescence profile at different pH in the range of 6 to 9 showed no structural variations for both cutinases, indicating the...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-1037-5
更新日期：2014-09-01 00:00:00
abstract：:Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enzyme properties by i...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-009-8720-y
更新日期：2010-09-01 00:00:00
abstract：:Qualitative and quantitative DNA-based methods were applied to detect genetically modified foods in samples from markets in the Kingdom of Saudi Arabia. Two hundred samples were collected from Al-Qassim, Riyadh, and Mahdina in 2009 and 2010. GMOScreen 35S and NOS test kits for the detection of genetically modified org...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0405-x
更新日期：2013-10-01 00:00:00
abstract：:Low-cost dried yeasts immobilized on delignified brewers' spent grains for use in wine making and brewing were produced by simple thermal drying techniques. To optimize the thermal drying process, vacuum and air stream conditions were examined. Drying of thin layers of the biocatalysts was performed at low (30-38 degr...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-009-8848-9
更新日期：2010-09-01 00:00:00
abstract：:Previously, it was reported that human amniotic membrane (AM) induced stem cells from human deciduous exfoliated teeth (SHED) endothelial-like-cell differentiation. This interesting effect of AM matrix on SHED demands further elucidation. Objective of this in vitro work was to study the effect of 24-h VEGF induced on ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-020-03266-1
更新日期：2020-05-01 00:00:00
abstract：:Regulation of the cysteine protease activity is imperative for proper functioning of the various organ systems. Elevated activities of cysteine proteinases due to impaired regulation by the endogenous cysteine proteinase inhibitors (cystatins) have been linked to liver malignancies. To gain an insight into these regul...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0383-z
更新日期：2013-10-01 00:00:00
abstract：:IOGEN Corporation of Ottawa, Canada, has recently built a 40t/d biomass-to-ethanol demonstration plant adjacent to its enzyme production facility. It has partnered with the University of Toronto to test the C6/C5 cofermenta-tion performance characteristics of the National Renewable Energy Labora-tory's metabolically e...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:98-100:1-9:429
更新日期：2002-04-01 00:00:00
abstract：:Bacterial strain NYT501, which we previously isolated from soil, was identified as Stenotrophomonas maltophilia, and it was confirmed that this strain produces an intracellular β-N-acetylhexosaminidase exhibiting transglycosylation activity. Several properties of this enzyme were characterized using a partially purifi...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-017-2585-2
更新日期：2018-02-01 00:00:00
abstract：:A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjug...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02780797
更新日期：1981-09-01 00:00:00
abstract：:A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02788064
更新日期：1996-08-01 00:00:00
abstract：:Five autotrophic strains isolated from non-photosynthetic microbial communities (NPMCs), which were screened from oceans with high CO2 fixing capability, were identified as Ochrobactrum sp. WH-2, Stenotrophomonas sp. WH-11, Ochrobactrum sp. WH-13, Castellaniella sp. WH-14, and Sinomicrobium oceani WH-15. The CO2 fixat...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1657-4
更新日期：2015-07-01 00:00:00
abstract：:Bacillus amyloliquefaciens LL3 is a glutamate-independent poly-γ-glutamic acid (γ-PGA) producing strain which consists of a circular chromosome (3,995,227 bp) and an endogenous plasmid pMC1 (6,758 bp). The study of the function of native plasmid and the genome-size reduction of the B. amyloliquefaciens LL3 strain requ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0382-0
更新日期：2013-09-01 00:00:00
abstract：:Titanium-dioxide nanoparticles (TNP) are used in various consumable goods. Evidence has demonstrated the cytotoxicity of TNPs, but exact mechanism is yet to be elucidated. The present study has been aimed at finding out the mechanism of TNP-induced toxicity in biological system. Different doses of anatase-TNPs adminis...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2165-x
更新日期：2016-12-01 00:00:00
abstract：:Commercial conversion of lignocellulosic biomass to fermentable sugars requires inexpensive bulk production of biologically active cellulase enzymes, which might be achieved through direct production of these enzymes within the biomass crops. Transgenic corn plants containing the catalytic domain of Acidothermus cellu...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-007-9053-3
更新日期：2007-04-01 00:00:00
abstract：:The glpX gene (Rv1099c) of Mycobacterium tuberculosis (Mtb) encodes Fructose 1,6-bisphosphatase II (FBPase II; EC 3.1.3.11); a key gluconeogenic enzyme. Mtb possesses glpX homologue as the major known FBPase. This study explored the expression, purification and enzymatic characterization of functionally active FBPase ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-011-9219-x
更新日期：2011-08-01 00:00:00
abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain its stability due to high temperature during the feed pelleting process. In this study, we performed site-directed mutagenesis in the Aspergil...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0380-2
更新日期：2013-10-01 00:00:00
abstract：:Microalgae have been proposed as a potential feedstock for biofuel production; however, cell disruption is usually required for collection and utilization of cytoplasmic polysaccharides and lipids. Virus infection might be one approach to disrupt the cell wall. The concentration of yeast extract and presence of KNO3 i...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1588-0
更新日期：2015-05-01 00:00:00
