Production of insoluble exopolysaccharide of Agrobacterium sp. (ATCC 31749 and IFO 13140).

abstract：:Artemisinin is an important drug commonly used in the treatment of malaria as a combination therapy. It is primarily produced by a plant Artemisia annua, however, its supply from plant is significantly lower than its huge demand and therefore alternative in vitro production routes are sought. Hairy root cultivation co...

journal_title：Applied biochemistry and biotechnology

authors： Patra N,Srivastava AK

更新日期：2014-11-01 00:00:00

abstract：:The use of viruses for treatment of cancer overcomes the bottlenecks of chemotherapy and radiotherapy. Several viruses and their proteins have been evaluated for oncolytic effect. The VP3 protein (apoptin) of chicken anemia virus is one such protein with an inherent ability to lyse cancer and transformed cells while l...

journal_title：Applied biochemistry and biotechnology

authors： Singh PK,Tiwari AK,Rajmani RS,Kumar GR,Chaturvedi U,Saxena L,Saxena S,Doley J,Sahoo AP,Santra L,Saxena M,Kumar S,Sharma B

更新日期：2015-05-01 00:00:00

abstract：:Proinflammatory cytokines such as tumor necrosis factor (TNF) alpha are well known to inhibit adipocyte differentiation. TNF-alpha triggers ceramide synthesis through binding of TNF-alpha to its p55 receptor. Therefore, ceramide is implicated in many of the multiple signaling pathways initiated by TNF-alpha. In breast...

journal_title：Applied biochemistry and biotechnology

authors： Xu F,Yang CC,Gomillion C,Burg KJ

更新日期：2010-01-01 00:00:00

abstract：:The host-pathogen interaction and involvement of calcium (Ca2+) signaling in tuberculosis infection is crucial and plays a significant role in pathogenesis. Ca2+ is known as a ubiquitous second messenger that could control multiple processes and is included in cellular activities like division, motility, stress respon...

journal_title：Applied biochemistry and biotechnology

authors： Sharma S,Meena LS

更新日期：2017-02-01 00:00:00

abstract：:Biomass degradation compounds significantly inhibit biochemical conversion of biomass prehydrolysates to biofuels and chemicals, such as lactic acid. To characterize the structure-activity relationship of carbonyl inhibition on lactic acid fermentation, we examined effects of eight carbonyl compounds (furfural, 5-hydr...

journal_title：Applied biochemistry and biotechnology

authors： Li J,Zhu C,Tu M,Han P,Wu Y

更新日期：2015-04-01 00:00:00

abstract：:L-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the L-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The im...

journal_title：Applied biochemistry and biotechnology

authors： Kim KR,Seo ES,Oh DK

更新日期：2014-01-01 00:00:00

abstract：:The performance of immobilized Bifidobacterium longum in sodium alginate beads and on a spiral-sheet bioreactor for the production of lactic acid from cheese whey was evaluated. Lactose utilization and lactic acid yield of B. longum were compared with those of Lactobacillus helveticus. B. longum immobilized in sodium ...

journal_title：Applied biochemistry and biotechnology

authors： Shahbazi A,Mims MR,Li Y,Shirley V,Ibrahim SA,Morris A

更新日期：2005-04-01 00:00:00

abstract：:In a recombinant antibody scFv format, the presence of an unpaired cysteine (Cys) is implicated in reduced soluble expression and inefficient presentation in phage display. Compared to other species, antibodies derived from rabbits are more likely to contain this unpaired Cys residue at position 80 (Cys80), when gener...

journal_title：Applied biochemistry and biotechnology

authors： Ayyar BV,Hearty S,O'Kennedy R

更新日期：2018-07-01 00:00:00

abstract：:For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expressi...

journal_title：Applied biochemistry and biotechnology

authors： Xiao B,Li Q,Feng B,Han Z,Gao D,Zhao R,Li J,Li K,Zhi X,Yang H,Liu Z

更新日期：2009-06-01 00:00:00

abstract：:Cancer is one of the killer diseases in humans and needs alternate curative measures despite recent improvement in modern treatment modalities. Oncolytic virotherapy seems to be a promising nonconventional way to treat cancers. Newcastle disease virus (NDV), a poultry virus, is nonpathogenic to human and domestic anim...

journal_title：Applied biochemistry and biotechnology

authors： Kumar R,Tiwari AK,Chaturvedi U,Kumar GR,Sahoo AP,Rajmani RS,Saxena L,Saxena S,Tiwari S,Kumar S

更新日期：2012-08-01 00:00:00

abstract：:The kinetic parameters of the oxidant action of the combination of enzyme horseradish peroxidase (HRP) with hydrogen peroxide in the degradation of methylene blue dye were investigated. Twenty-one percent of color removal was obtained at pH 5.0 and temperature of 30 °C. Under these conditions, the kinetic parameters K...

journal_title：Applied biochemistry and biotechnology

authors： Pereira AR,da Costa RS,Yokoyama L,Alhadeff EM,Teixeira LA

更新日期：2014-12-01 00:00:00

abstract：:A conducting poly(pyrrole-co-pyrrolepropylic acid) copolymer nanocomposite film (AuNP-PPy-PPa) incorporating gold nanoparticles (AuNP) was electrochemically grown using a single step procedure over electrochemically reduced graphene oxide (RGO) flakes deposited on a silane-modified indium-tin-oxide (ITO) glass plate. ...

journal_title：Applied biochemistry and biotechnology

authors： Puri N,Niazi A,Srivastava AK,Rajesh

更新日期：2014-10-01 00:00:00

abstract：:In this work, the catalytic conversion of natural polymer chitosan to 5-HMF under low temperature hydrothermal condition was investigated. The highest 5-HMF yield of 12.1 wt% was obtained under the following conditions: 174 °C reaction temperature, 2.2 % catalyst amount, and 36.9 min reaction time. In the effect of co...

journal_title：Applied biochemistry and biotechnology

authors： Lee SB,Jeong GT

更新日期：2015-06-01 00:00:00

abstract：:Different treatments to improve the thermotolerance of fermenting yeasts for simultaneous ethanol saccharification and fermentation process of cellulosic materials have been examined. Yeasts of the genera Saccharomyces and Kluyveromyces were tested for growth and fermentation at progressively higher temperatures in th...

journal_title：Applied biochemistry and biotechnology

authors： Ballesteros I,Oliva JM,Ballesteros M,Carrasco J

更新日期：1993-04-01 00:00:00

abstract：:The cellulolytic enzymes from filamentous fungi are widely used for production of biofuels. Molecular biological engineering of fungal strains has been applied to improve the cellulase production in the recent years. The VIB gene affects production of cellulase and known as a new transcription factor. Based on a fast-...

journal_title：Applied biochemistry and biotechnology

authors： Han J,Xue Y,Li M,Li Y,Liu J,Gan L,Long M

更新日期：2020-08-01 00:00:00

abstract：:Further characterization of an aspartyl protease from Mucor bacilliformis with milk-clotting activity was performed. An extinction coefficient, epsilon 278 cm = 1.61 mL/mg/cm, a molecular mass of 35,400 Da and a pI of 5.2 were determined. Proteolytic activity and kinetic parameters were evaluated by using the hexapept...

journal_title：Applied biochemistry and biotechnology

authors： Venera GD,Machalinski C,Zumárraga H,Biscoglio de Jiménez Bonino MJ

更新日期：1997-12-01 00:00:00

abstract：:In the present work, assays were performed to compare the efficacy of hypochlorous acid, chlorine dioxide, and ozone in the inactivation of Cryptosporidium oocyst in public water supply from Brazilian South conditions. Experiments were carried out in samples containing 2 x 10(4) oocysts/ml of C. parvum purified from f...

journal_title：Applied biochemistry and biotechnology

authors： Pereira JT,Costa AO,de Oliveira Silva MB,Schuchard W,Osaki SC,de Castro EA,Paulino RC,Soccol VT

更新日期：2008-12-01 00:00:00

abstract：:The increasing death rates related to anaplastic lymphoma kinase (ALK)-positive lung cancer culminated in a significant interest in the discovery of novel inhibitors for ALK. In the present research work, pharmacophore-based 3D QSAR modeling and virtual screening strategy have been carried out to address these issues....

journal_title：Applied biochemistry and biotechnology

authors： James N,Shanthi V,Ramanathan K

更新日期：2018-05-01 00:00:00

abstract：:A high cellobiohydrolase (CBH)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 according to the morphology and comparison of internal transcribed spacer rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, a maximum CBH activi...

journal_title：Applied biochemistry and biotechnology

authors： Lee KM,Joo AR,Jeya M,Lee KM,Moon HJ,Lee JK

更新日期：2011-01-01 00:00:00

abstract：:Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growt...

journal_title：Applied biochemistry and biotechnology

authors： Cai Z,Riedel H,Thaw Saw NM,Kütük O,Mewis I,Jäger H,Knorr D,Smetanska I

更新日期：2011-06-01 00:00:00

abstract：:In this study, the gene encoding an α-amylase from a psychrophilic Arthrobacter agilis PAMC 27388 strain was cloned into a pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3). The recombinant α-amylase with a molecular mass of about 80 kDa was purified by using Ni2+-NTA affinity chromatography...

journal_title：Applied biochemistry and biotechnology

authors： Kim SM,Park H,Choi JI

更新日期：2017-03-01 00:00:00

abstract：:Among 18 psychrophilic strains isolated near the Antarctic Station, the psychrophilic strain Rhodotorula aurantiaca A19 was selected for its ability of growth and gamma-decalactone production at low temperatures. The effects of temperature, initial pH, and castor oil concentration on the growth and gamma-decalactone p...

journal_title：Applied biochemistry and biotechnology

authors： Alchihab M,Destain J,Aguedo M,Majad L,Ghalfi H,Wathelet JP,Thonart P

更新日期：2009-07-01 00:00:00

abstract：:Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

journal_title：Applied biochemistry and biotechnology

authors： Padma PN,Rao AB,Yadav JS,Reddy G

更新日期：2002-07-01 00:00:00

abstract：:The white-rot fungus Trametes multicolor MB 49 has been identified as an excellent producer of the industrially important enzyme laccase. The formation of extracellular laccase could be considerably stimulated by the addition of Cu(II) to a simple, glycerol-based culture medium. In this study, optimal concentrations o...

journal_title：Applied biochemistry and biotechnology

authors： Hess J,Leitner C,Galhaup C,Kulbe KD,Hinterstoisser B,Steinwender M,Haltrich D

更新日期：2002-04-01 00:00:00

abstract：:We have previously demonstrated that pretreatment of corn stover with dilute sulfuric acid can achieve high digestibility and efficient recovery of hemicellulose sugars with high yield and concentration. Further improvement of this process was sought in this work. A modification was made in the operation of the percol...

journal_title：Applied biochemistry and biotechnology

authors： Zhu Y,Lee YY,Elander RT

更新日期：2005-04-01 00:00:00

abstract：:The antioxidant activity of a novel artificial glutathione peroxidase-like enzyme, selenium-containing glutathione 5-transferase from Lucilia cuprina (seleno-LuGST1-1), was studied by using a ferrous sulfate/ascorbate-induced mitochondrial damage model system. Swelling of mitochondria, lipid peroxidation, and cytochro...

journal_title：Applied biochemistry and biotechnology

authors： Yu H,Liu J,Li J,Zang T,Luo G,Shen J

更新日期：2005-11-01 00:00:00

abstract：:The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme. Km and turn over values of the enzyme were 6.25 mg/mL and 5.0 × 102/min, respectively. Effect of different metal i...

journal_title：Applied biochemistry and biotechnology

authors： Mulay YR,Deopurkar RL

更新日期：2018-02-01 00:00:00

abstract：:Oseltamivir (Tamiflu) is the preferred anti-viral drug employed to fight the flu virus in infected individuals. The principal target for this drug is a virus surface glycoprotein, neuraminidase (NA), which facilitates the release of nascent virus and thus spreads infections. Until recently, only a low prevalence of ne...

journal_title：Applied biochemistry and biotechnology

authors： Karthick V,Shanthi V,Rajasekaran R,Ramanathan K

更新日期：2012-05-01 00:00:00

abstract：:Mesoporous materials possess a hexagonal array of uniform mesopores, high surface areas, and moderate acidity. They are one of the important catalysts in the field of catalytic pyrolysis. In this paper, mesoporous materials of Al-MCM-41, La-Al-MCM-41, and Ce-Al-MCM-41 were synthesized, characterized, and tested as cat...

journal_title：Applied biochemistry and biotechnology

authors： Yu FW,Ji DX,Nie Y,Luo Y,Huang CJ,Ji JB

更新日期：2012-09-01 00:00:00

abstract：:In this study, we investigated the dynamics of a computer simulation of a continuous alcoholic fermentation process combined with a flash column under vacuum. The alcohol was partially extracted in order to maintain its concentration at about 40 kg/m3 in the fermentor. The mathematical model of the fermentation was de...

journal_title：Applied biochemistry and biotechnology

authors： Costa AC,Dechechi EC,Silva FL,Maugeri F,Maciel R

更新日期：2000-04-01 00:00:00