Effect of acetic acid on Saccharomyces carlsbergensis ATCC 6269 batch ethanol production monitored by flow cytometry.

abstract：:A genetically engineered Escherichia coli cell expressing both organophosphorus hydrolase (OPH) and carboxyl esterase (CaE) B1 intracellularly was constructed and cultivated. The harvested wet cells were vacuum dried, and the storage stability of the dried cell powder was determined in terms of OPH activity. Over a pe...

journal_title：Applied biochemistry and biotechnology

authors： Zheng YZ,Lan WS,Qiao CL,Mulchandani A,Chen W

更新日期：2007-03-01 00:00:00

abstract：:Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

journal_title：Applied biochemistry and biotechnology

authors： Ferreira MS,De Andrade AV,Kennedy JF

更新日期：1991-10-01 00:00:00

abstract：:Candida boidinii produces significant amounts of xylitol from xylose, and assays of crude homogenates for aldose (xylose) reductase (XYL1p) have been reported to show relatively high activity with NADH as a cofactor even though XYL1p purified from this yeast does not have such activity. A gene coding for XYL1p from C....

journal_title：Applied biochemistry and biotechnology

authors： Kang MH,Ni H,Jeffries TW

更新日期：2003-04-01 00:00:00

abstract：:Hypocrellin A production by Shiraia sp. SUPER-H168 was studied under solid-state fermentation. Corn was found to be the best substrate after evaluating eight kinds of agro-industrial crops and residues. The optimized solid-state fermentation conditions were as follows: inoculum size 3 x 10(6) spores, substrate particl...

journal_title：Applied biochemistry and biotechnology

authors： Cai Y,Liang X,Liao X,Ding Y,Sun J,Li X

更新日期：2010-04-01 00:00:00

abstract：:Enzymes from thermophilic microorganisms, thermozymes, have unique characteristics such as temperature, chemical, and pH stability. They can be used in several industrial processes, in which they replace mesophilic enzymes or chemicals. Thermozymes are often used when the enzymatic process is compatible with existing ...

journal_title：Applied biochemistry and biotechnology

authors： Bruins ME,Janssen AE,Boom RM

更新日期：2001-02-01 00:00:00

abstract：:This investigation utilized sugarcane bagasse aqueous extract (SBAE), a nontoxic, cost-effective medium to boost triacylglycerol (TAG) accumulation in novel fresh water microalgal isolate Scenedesmus sp. IITRIND2. Maximum lipid productivity of 112 ± 5.2 mg/L/day was recorded in microalgae grown in SBAE compared to mod...

journal_title：Applied biochemistry and biotechnology

authors： Arora N,Patel A,Pruthi PA,Pruthi V

更新日期：2016-09-01 00:00:00

abstract：:A multi-method protocol previously proposed for the extraction of extracellular polymeric substances (EPS) from flocculated sludges was investigated on dense aerobic granules. The protocol combines mechanical disruption by sonication and chemical extraction using the Tween detergent and the cation chelator, EDTA. Poly...

journal_title：Applied biochemistry and biotechnology

authors： Caudan C,Filali A,Lefebvre D,Spérandio M,Girbal-Neuhauser E

更新日期：2012-04-01 00:00:00

abstract：:A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protecti...

journal_title：Applied biochemistry and biotechnology

authors： Marques ET Jr,Lima Filho JL

更新日期：1992-01-01 00:00:00

abstract：:A 28-day feeding experiment with formulated feed using docosahexaenoic acid (DHA)-rich whole cells of freeze-dried marine microalgae Schizochytrium sp. to understand the distribution of fatty acids in a laboratory model zebrafish was conducted. Three feeds, commercial feed, 50:50 feed (50% commercial and 50% algae), a...

journal_title：Applied biochemistry and biotechnology

authors： Byreddy AR,Yoganantharjah P,Gupta A,Gibert Y,Puri M

更新日期：2019-05-01 00:00:00

abstract：:Efficient degradation of cellulose needs a synergistic reaction of the cellulolytic enzymes, which include exoglucanases, endoglucanases, and β-1,4-glucosidase. In this study, we used an improved Bac-to-Bac/BmNPV baculovirus expression system, which lacks the virus-encoded chitinase cathepsin (v-cath) genes of Bombyx ...

journal_title：Applied biochemistry and biotechnology

authors： Li XH,Wang MX,Zhang P,Hu JB,Sun CG,Liu XJ,Zhou F,Niu YS,Malik FA,Bhaskar R,Yang HJ,Miao YG

更新日期：2011-09-01 00:00:00

abstract：:Microalgae farming has been identified as the most eco-sustainable solution for producing biodiesel. However, the operation of full-scale plants is still limited by costs and the utilization of industrial and/or domestic wastes can significantly improve economic profits. Several waste effluents are valuable sources of...

journal_title：Applied biochemistry and biotechnology

authors： Marques SS,Nascimento IA,de Almeida PF,Chinalia FA

更新日期：2013-12-01 00:00:00

abstract：:The autoimmune repertoire is well known from previous studies to be capable of producing catalytic antibodies directed to self-antigens. In the present study, we explored the ability of 26 monoclonal light chains (L chains) from multiple myeloma patients to cleave radiolabeled gp120, a foreign protein. One L chain wit...

journal_title：Applied biochemistry and biotechnology

authors： Paul S,Kalaga RS,Gololobov G,Brenneman D

更新日期：2000-01-01 00:00:00

abstract：:A comparative analysis of the structural and functional aspects along with equilibrium unfolding of two homologous cutinases, Cut1 and Cut2, from Thermobifida fusca was carried out. The CD and fluorescence profile at different pH in the range of 6 to 9 showed no structural variations for both cutinases, indicating the...

journal_title：Applied biochemistry and biotechnology

authors： Hegde K,Dasu VV

更新日期：2014-09-01 00:00:00

abstract：:A new lipase from seeds of Pachira aquatica was purified to homogeneity by SDS-PAGE obtaining an enzyme with a molecular weight of approximately 55 kDa. The purified lipase exhibited maximum activity at 40 degrees C and pH 8.0, for an incubation time of 90 min. Concerning temperature stability, at the range from 4 to ...

journal_title：Applied biochemistry and biotechnology

authors： Polizelli PP,Facchini FD,Cabral H,Bonilla-Rodriguez GO

更新日期：2008-09-01 00:00:00

abstract：:This work reports the isolation of a serum lectin from cobia fish (Rachycentron canadum) named RcaL. Immunomodulatory activity on mice splenocyte experimental cultures through cytotoxic assays and cytokine production were also performed. RcaL was obtained through precipitation with ammonium sulphate and affinity chrom...

journal_title：Applied biochemistry and biotechnology

authors： Coriolano MC,Silva CD,Melo CM,Bezerra Rde S,Santos AJ,Pereira VR,Coelho LC

更新日期：2012-11-01 00:00:00

abstract：:Proteases constitute one of the most important groups of industrial enzymes, accounting for at least 25% of the total enzyme sales, with two-thirds of the proteases produced commercially being of microbial origin. Immobilized enzymes are currently the subject of considerable interest because of their advantages over s...

journal_title：Applied biochemistry and biotechnology

authors： Kirkkopru I,Alpaslan C,Omay D,Güvenilir Y

更新日期：2006-04-01 00:00:00

abstract：:A conducting poly(pyrrole-co-pyrrolepropylic acid) copolymer nanocomposite film (AuNP-PPy-PPa) incorporating gold nanoparticles (AuNP) was electrochemically grown using a single step procedure over electrochemically reduced graphene oxide (RGO) flakes deposited on a silane-modified indium-tin-oxide (ITO) glass plate. ...

journal_title：Applied biochemistry and biotechnology

authors： Puri N,Niazi A,Srivastava AK,Rajesh

更新日期：2014-10-01 00:00:00

abstract：:Dissolved oxygen (DO) is an imperative parameter of the activated sludge process (ASP) for wastewater bioremediation. The effect of DO on microbial communities and corresponding metabolic functions in wastewater bioremediation was investigated using next-generation analysis techniques in this study. Illumina-based who...

journal_title：Applied biochemistry and biotechnology

authors： Yadav TC,Jadeja NB,Kapley A

更新日期：2020-10-01 00:00:00

abstract：:Penicillin V acylase (PVA) is a pharmaceutically important enzyme as it plays a vital role in the manufacture of semi-synthetic beta-lactam antibiotics. Rhodotorula aurantiaca (NCIM 3425) produced high levels of intracellular penicillin V acylase after 18 h at pH 8.0 and temperature 27 degrees C. Fructose was the best...

journal_title：Applied biochemistry and biotechnology

authors： Kumar A,Singh S,Poddar P,Prabhune A,Pundle A

更新日期：2009-06-01 00:00:00

abstract：:A combined process, de-algination followed by enzymatic saccharification, was designed to produce alginate and glucose from Saccharina japonica consecutively. The process conditions of de-algination were optimized separately for each stage of acidification and alkaline extraction. Collectively, the de-algination yield...

journal_title：Applied biochemistry and biotechnology

authors： Ryu HJ,Oh KK

更新日期：2017-05-01 00:00:00

abstract：:Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

journal_title：Applied biochemistry and biotechnology

authors： Liu K,Chua H,Lo WH,Lawford H,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:A protein-engineered beta-lactamase, constructed by site-directed mutagenesis in Escherichia coli (E104M/G238S), and having broadened specificity, was able to degrade cephalosporins of first, second, and third generations. Manipulations of culture conditions allowed an increase in beta-lactamase specific activity by u...

journal_title：Applied biochemistry and biotechnology

authors： García JL,Nuñez CJ,González EG,Osuna J,Soberón X,Galindo E

更新日期：1998-05-01 00:00:00

abstract：:Rice straw hemicellulosic hydrolysate was used as fermentation medium for ethanol production by Pichia stipitis NRRL Y-7124. Shaking bath experiments were initially performed aiming to establish the best initial xylose concentration to be used in this bioconversion process. In the sequence, assays were carried out und...

journal_title：Applied biochemistry and biotechnology

authors： Silva JP,Mussatto SI,Roberto IC

更新日期：2010-11-01 00:00:00

abstract：:An integrated biodiesel process that combines enzymatic esterification and alkaline transesterification is suggested. With focus on the enzymatic step, the paper provides proof of concept and suggestions for further process development. Hence, palm fatty acid distillate (PFAD) has been enzymatically converted to fatty...

journal_title：Applied biochemistry and biotechnology

authors： Brask J,Damstrup ML,Nielsen PM,Holm HC,Maes J,De Greyt W

更新日期：2011-04-01 00:00:00

abstract：:Amphiphilic polyurethane (APU) nano-particle enhanced the mobilization of 2-methylnaphthalene (2-MNPT) in soil. Significant increase in the solubility of 2-MNPT was achieved. The molar solubilization ratio was 0.4 (mole 2-MNPT/mole APU). Simple precipitation of APU particle by 2 N CaCl(2) recovered 95% of APU particle...

journal_title：Applied biochemistry and biotechnology

authors： Kim YB,Kim JY,Kim EK

更新日期：2009-10-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:The ability of non-living biomass of an arsenic-hypertolerant Bacillus cereus strain SZ2 isolated from a gold mining environment to adsorb As (III) from aqueous solution in batch experiments was investigated as a function of contact time, initial As (III) concentration, pH, temperature and biomass dosage. Langmuir mod...

journal_title：Applied biochemistry and biotechnology

authors： Mohd Bahari Z,Ali Hamood Altowayti W,Ibrahim Z,Jaafar J,Shahir S

更新日期：2013-12-01 00:00:00

abstract：:Gold nanomaterials (Au NMs) have attracted increasing attention in biomedicine due to their facile preparation, multifunctional modifications, unique optical and electrical properties, and good biocompatibility. The physicochemical properties of Au NMs at nanoscale, like size, shape, surface chemistry, and near field ...

journal_title：Applied biochemistry and biotechnology

authors： Jiang XM,Wang LM,Wang J,Chen CY

更新日期：2012-03-01 00:00:00

abstract：:The inhibitory effect of L-malic acid (MA) on alpha-glucosidase (EC 3.2.1.20) was investigated by combination study between inhibition kinetics and computational simulations. The results from the serial kinetics demonstrated that MA could directly inactivate the enzyme activity in a dose-dependent manner and a typical...

journal_title：Applied biochemistry and biotechnology

authors： Gou L,Zhan Y,Lee J,Li X,Lü ZR,Zhou HM,Lu H,Wang XY,Park YD,Yang JM

更新日期：2015-02-01 00:00:00