Activity and lifetime of urease immobilized using layer-by-layer nano self-assembly on silicon microchannels.

abstract：:Outdoor production of microalgae could be set on a sound industrial basis if solar energy were utilized at a much higher efficiency than presently obtained. Many types of photobioreactors have been developed in the past in an attempt to answer this challenge, but their photosynthetic efficiency has been rather similar...

journal_title：Applied biochemistry and biotechnology

authors： Richmond A,Qiang H

更新日期：1997-04-01 00:00:00

abstract：:Alginate lyases have been widely used for the preparation of bioactive alginate oligosaccharides. An alginate lyase AlgL-CD was rationally designed by introducing alkaline amino acid residues near active center to increase activity. One of its mutants E226K presented much higher activity than wild-type AlgL-CD. Substr...

journal_title：Applied biochemistry and biotechnology

authors： Xu X,Zeng D,Wu D,Lin J

更新日期：2021-01-23 00:00:00

abstract：:This study deals with the utilization of chicken feather waste as a substrate for anaerobic digestion and improving biogas production by degradation of the compact structure of the feather keratin. In order to increase the digestibility of the feather, different pretreatments were investigated, including thermal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Forgács G,Lundin M,Taherzadeh MJ,Sárvári Horváth I

更新日期：2013-04-01 00:00:00

abstract：:NADH : FMN oxidoreductase and bacterial luciferase have been efficiently coimmobilized onto Sepharose 4B. This luminescent immobilized enzyme system can be used to assay NADH. The assay is rapid and sensitive with a lower limit of detection of 0.2 pmol/assay tube. The intra-assay precision was 3.5% at 2 × 10(-5) M and...

journal_title：Applied biochemistry and biotechnology

authors： Wienhausen GK,Kricka LJ,Hinkley JE,Deluca M

更新日期：1982-11-01 00:00:00

abstract：:To develop more potent thrombolytic agents with fibrinolytic and antiplatelet aggregation activity, staphylokinase (Sak) variant Y1-Sak, a recombinant mutant of the Staphylococcus aureus protein Sak, was constructed. Y1-Sak formed an insoluble inclusion body when overexpressed in Escherichia coli strain JF1125. To obt...

journal_title：Applied biochemistry and biotechnology

authors： He J,Wang G,Xu R,Feng J,Wang J,Su H,Song H

更新日期：2008-10-01 00:00:00

abstract：:Renal calculi disease or known as kidney stone disease is the most common urological disorder in both men and women, although it is more prevalent in men. The lifetime chance for an individual to develop renal calculi is approximately 10% whereas the risk of recurrence in a 10-year period is 74%. Therefore, a diagnost...

journal_title：Applied biochemistry and biotechnology

authors： Wai-Hoe L,Wing-Seng L,Ismail Z,Lay-Harn G

更新日期：2009-10-01 00:00:00

abstract：:Microalgae have been proposed as a potential feedstock for biofuel production; however, cell disruption is usually required for collection and utilization of cytoplasmic polysaccharides and lipids. Virus infection might be one approach to disrupt the cell wall. The concentration of yeast extract and presence of KNO3 i...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YS,Labavitch J,VanderGheynst JS

更新日期：2015-05-01 00:00:00

abstract：:Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from ei...

journal_title：Applied biochemistry and biotechnology

authors： Thierfelder S,Ostermann K,Göbel A,Rödel G

更新日期：2011-04-01 00:00:00

abstract：:Rapid, accurate, and easy identification of pathogenic agents has always been important in medicine, veterinary, and agriculture. The brown spot infection is among the most common diseases in tangerine caused by Alternaria alternata. Due to the existence of seven various pathotypes of A. alternata species, it is chall...

journal_title：Applied biochemistry and biotechnology

authors： Moghimi H,Moradi A,Hamedi J,Basiri M

更新日期：2016-03-01 00:00:00

abstract：:Indole-3-carbinol (I3C) is a naturally occurring glucosinolate found in Brassica vegetables that is usually converted in gastric acidic environment to the efficient metabolite 3,3'-diindolylmethane (DIM). Both indoles (I3C and DIM) are known chemopreventive agents for various cancers including breast cancer. This stud...

journal_title：Applied biochemistry and biotechnology

authors： El-Daly SM,Gamal-Eldeen AM,Gouhar SA,Abo-Elfadl MT,El-Saeed G

更新日期：2020-12-01 00:00:00

abstract：:Glucoamylase from the thermophilic mold Thermomucor indicae-seudaticae was purified by anion exchange and gel filtration chromatographic techniques using a fast protein liquid chromatographic system. The structure and thermal stability of this unique 'thermostable and neutral glucoamylase' were analyzed by circular di...

journal_title：Applied biochemistry and biotechnology

authors： Kumar P,Islam A,Ahmad F,Satyanarayana T

更新日期：2010-03-01 00:00:00

abstract：:The common view of amyloids and prion proteins is that they are associated with many currently incurable diseases and present a great danger to an organism. This danger comes from the fact that not only prion proteins, but also the infectious form(s) of amyloids, as it has been shown recently, are able to transmit the...

journal_title：Applied biochemistry and biotechnology

authors： Pulawski W,Ghoshdastider U,Andrisano V,Filipek S

更新日期：2012-04-01 00:00:00

abstract：:Fluorescence spectroscopy, UV-visible absorption spectroscopy, circular dichroism (CD) spectroscopy, viscometry, cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were applied to investigate the competitive interaction of DNA with two aromatic α-aminobisphosphonates and neutral red dye (NR, intercalato...

journal_title：Applied biochemistry and biotechnology

authors： Gholivand MB,Peyman H,Gholivand K,Roshanfekr H,Taherpour AA,Yaghobi R

更新日期：2017-07-01 00:00:00

abstract：:Modification of the phosphoenolpyruvate/sugar phosphotransferase system (PTS) has shown improvement in sugar coassimilation in Escherichia coli production strains. However, in preliminary experiments under anaerobic conditions, E. coli strains with an inactive PTS and carrying pLOI1594, which encodes pyruvate decarbox...

journal_title：Applied biochemistry and biotechnology

authors： Balderas-Hernández VE,Hernández-Montalvo V,Bolívar F,Gosset G,Martínez A

更新日期：2011-02-01 00:00:00

abstract：:In addition to fermentable sugars, dilute-acid hydrolysates of lignocellulose contain compounds that inhibit fermenting microorganisms, such as Saccharomyces cerevisiae. Previous results show that phenolic compounds and furan aldehydes, and to some extent aliphatic acids, act as inhibitors during fermentation of dilut...

journal_title：Applied biochemistry and biotechnology

authors： Nilvebrant NO,Persson P,Reimann A,De Sousa F,Gorton L,Jönsson LJ

更新日期：2003-04-01 00:00:00

abstract：:Streptomyces griseorubens JSD-1 is a novel actinomycete that could grow efficiently upon lignin, and the ligninolytic genes active in this biotransformation were expected to be crucial. To investigate the molecular mechanism of utilizing lignin, genome sequencing was carried out to obtain its draft genome, which was d...

journal_title：Applied biochemistry and biotechnology

authors： Feng H,Zhang D,Sun Y,Zhi Y,Mao L,Luo Y,Xu L,Wang L,Zhou P

更新日期：2015-05-01 00:00:00

abstract：:Trehalose synthase (TreS) from Meiothermus ruber was co-aggregated with polyethyleneimine (PEI) and precipitated with polyethylene glycol (PEG), followed by cross-linking with glutaraldehyde to obtain TreS-polyethyleneimine cross-linked enzyme aggregates (termed as CLEAs-PEI-PEG). The TreS solution at 0.5 mg mL(-1) pr...

journal_title：Applied biochemistry and biotechnology

authors： Zheng J,Chen Y,Yang L,Li M,Zhang J

更新日期：2014-11-01 00:00:00

abstract：:(R)-2-Phenoxy-propionic acid methyl ester (PPAM) is an important chiral precursor of aryloxy phenoxy propionate herbicides. The covalent immobilization of lipase from Aspergillus oryzae WZ007 and the catalysis of enantioselective (R, S)-PPAM resolution by the immobilized A. oryzae lipase (AOL) were investigated in thi...

journal_title：Applied biochemistry and biotechnology

authors： Zhong W,Zhang M,Li X,Zhang Y,Wang Z,Zheng J

更新日期：2020-03-01 00:00:00

abstract：:A novel mannan-specific lectin was isolated from the roots of a traditional Chinese herbal medicine, Ophioglossum pedunculosum through ion-exchange chromatography and gel filtration. With a molecular mass of 19,835.7 Da demonstrated by MALDI-TOF analysis, this novel agglutinin was designated as O. pedunculosum aggluti...

journal_title：Applied biochemistry and biotechnology

authors： He XM,Ji N,Xiang XC,Luo P,Bao JK

更新日期：2011-12-01 00:00:00

abstract：:Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...

journal_title：Applied biochemistry and biotechnology

authors： Clark KJ,Harcum SW,Griffiths J,Bailey KM

更新日期：2005-06-01 00:00:00

abstract：:Laccase is one of the lignolytic enzymes found in liquid cultures of the fungus Coriolus zonatus in defined medium. The enzyme was isolated from culture liquid and characterized. Laccase from C. zonatus is a single-chain protein with a molecular mass of 60 kDa. Carbohydrate moiety of enzyme consisted of mannose, galac...

journal_title：Applied biochemistry and biotechnology

authors： Koroljova OV,Stepanova EV,Gavrilova VP,Biniukov VI,Jaropolov AI,Varfolomeyev SD,Scheller F,Makower A,Otto A

更新日期：1999-02-01 00:00:00

abstract：:An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprot...

journal_title：Applied biochemistry and biotechnology

authors： Batista FR,Greco KN,Astray RM,Jorge SA,Augusto EF,Pereira CA,Mendonça RZ,Moraes AM

更新日期：2011-01-01 00:00:00

abstract：:The vegetative insecticidal proteins (VIPs) of Bacillus thuringiensis (Bt) have a broad-spectrum insecticidal activity against Lepidopteran pests and no cross-resistance with the insecticidal crystal protein Cry protein. So there are great potentials for the control of agricultural pests and the resolution of resistan...

journal_title：Applied biochemistry and biotechnology

authors： Chi B,Li H,Zhang J,Wei P,Gao J,Liu R

更新日期：2019-04-01 00:00:00

abstract：:New approaches to veterinary drug screening based on liquid chromatography-mass spectrometry (LC-MS/MS) and time-of-flight mass spectrometry (ToF/MS) are rapid and have high selectivity and sensitivity. In this study, we developed a multiresidue method for screening over 100 veterinary drug residues using ion trap (IT...

journal_title：Applied biochemistry and biotechnology

authors： Kang J,Park SJ,Park HC,Hossain MA,Kim MA,Son SW,Lim CM,Kim TW,Cho BH

更新日期：2017-06-01 00:00:00

abstract：:Biotechnology is consistently seeking improved enzyme stability. Enzymes have great properties, although their marginal stability limits their applications. Among the strategies for improving stability of the enzymes, chemical modification is a simple and effective technique. In the present study, chemical modificatio...

journal_title：Applied biochemistry and biotechnology

authors： Hassani L,Nourozi R

更新日期：2014-04-01 00:00:00

abstract：:Fatty acid methyl esters (FAMEs) show large potential applications as diesel substitutes, also known as biodiesel fuel. Biodiesel fuel as renewable energy is an alternative that can reduce energy dependence on petroleum as well as air pollution. Several processes for the production of biodiesel fuel have been develope...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH,Kang CH,Lee WT,Sunwoo CS,Yoon CH,Choi BC,Kim HS,Kim SW,Lee UT

更新日期：2004-04-01 00:00:00

abstract：:To prevent illegal use of clenbuterol and for quality control in the food industry, more efficient and reliable methods for clenbuterol detection are needed. In this study, clenbuterol was detected using a spectral imaging surface plasmon resonance sensor system via two inhibition methods: (1) the target site compensa...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Yao M,Fang X,Yang Y,Cheng X

更新日期：2015-11-01 00:00:00

abstract：:Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL-1) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The p...

journal_title：Applied biochemistry and biotechnology

authors： Shivlata L,Satyanarayana T

更新日期：2017-04-01 00:00:00

abstract：:Empty palm fruit bunch fiber (EPFBF), a readily available cellulosic biomass from palm processing facilities, is investigated as a potential carbohydrate source for cellulosic ethanol production. This feedstock was pretreated using ammonia fiber expansion (AFEX) and enzymatically hydrolyzed. The best tested AFEX condi...

journal_title：Applied biochemistry and biotechnology

authors： Lau MJ,Lau MW,Gunawan C,Dale BE

更新日期：2010-11-01 00:00:00

abstract：:The enzyme 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGR) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. This enzyme has three domains: transmembrane, binding, and soluble. In this study, we expressed and puri...

journal_title：Applied biochemistry and biotechnology

authors： Andrade-Pavón D,Cuevas-Hernández RI,Trujillo-Ferrara JG,Hernández-Rodríguez C,Ibarra JA,Villa-Tanaca L

更新日期：2017-08-01 00:00:00