Comparison of the coupling recoveries of immobilized aspartate aminotransferase. Specific activity, enzyme-bound coenzyme, and transaminationable active centers.

Abstract:

:Aspartate aminotransferase (AspAT, EC 2.6.1.1) was bound on CNBr-activated Sepharose and the effects of immobilization on the maximum velocity, biologically active pyridoxal-5'-phosphate (PLP), and transaminationable active centers were studied. By comparing these parameters of soluble and immobilized enzyme the factors decreasing the observed reaction rate upon immobilization were evaluated. Ninety percent of the soluble protein in the coupling mixture was bound to the support. The amount of enzyme-bound PLP of immobilized preparation was 83% of that of the soluble one. The coupling recovery of specific activity was 46%, which was 10%-units lower than that of the transaminationable active centers. This difference depends on the fact that a part of the active centers of immobilized enzyme had lower catalytic rate, due to the enzyme-matrix interactions or internal mass transfer limitations, than the others. The immobilized catalytically active AspAT had 80% of the turnover efficiency of the soluble enzyme. The affinity of the enzyme to its substrates did not significantly change upon immobilization, neither did the pH profile.

authors

Kurkijärvi K,Korpela T

doi

10.1007/BF02778094

subject

Has Abstract

pub_date

1983-04-01 00:00:00

pages

135-44

issue

2

eissn

0273-2289

issn

1559-0291

journal_volume

8

pub_type

杂志文章
  • Chitosan-alginate microcapsules for oral delivery of egg yolk immunoglobulin (IgY): effects of chitosan concentration.

    abstract::In our previous study, chitosan-alginate microcapsules were developed to protect egg yolk immunoglobulin (IgY) from gastric inactivation. The present study was undertaken to determine the effect of chitosan concentration (0-0.8%; w/v) on various properties of the microcapsules in order to produce the optimum chitosan-...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8628-6

    authors: Li XY,Jin LJ,Lu YN,Zhen YH,Li SY,Wang LH,Xu YP

    更新日期:2009-12-01 00:00:00

  • Selection of microalgae and cyanobacteria strains for bicarbonate-based integrated carbon capture and algae production system.

    abstract::Using microalgae to capture CO2 from flue gas is an ideal way to reduce CO2 emission, but this is challenged by the high cost of carbon capture and transportation. To address this problem, a bicarbonate-based integrated carbon capture and algae production system (BICCAPS) has been proposed, in which bicarbonate is use...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0515-5

    authors: Chi Z,Elloy F,Xie Y,Hu Y,Chen S

    更新日期:2014-01-01 00:00:00

  • Hydrogen and methane production, energy recovery, and organic matter removal from effluents in a two-stage fermentative process.

    abstract::This study evaluates the potential for using different effluents for simultaneous H(2) and CH(4) production in a two-stage batch fermentation process with mixed microflora. An appreciable amount of H(2) was produced from parboiled rice wastewater (23.9 mL g(-1) chemical oxygen demand [COD]) and vinasse (20.8 mL g(-1) ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9807-4

    authors: Peixoto G,Pantoja-Filho JL,Agnelli JA,Barboza M,Zaiat M

    更新日期:2012-10-01 00:00:00

  • Improvement of bioethanol productivity of immobilized Saccharomyces bayanus with using sodium alginate-graft-poly(N-vinyl-2-pyrrolidone) matrix.

    abstract::In this study, immobilization conditions and bioethanol production characteristics of immobilized Saccharomyces bayanus were investigated into sodium alginate-graft-poly(N-vinyl-2-pyrrolidone; NaAlg-g-PVP) matrix. The matrix that crosslinked with calcium clorid was used for immobilization of S. bayanus. Bioethanol pro...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9770-0

    authors: İnal M,Yiğitoğlu M

    更新日期:2012-09-01 00:00:00

  • Site-Directed Mutagenesis of Cytochrome P450 2D6 and 2C19 Enzymes: Expression and Spectral Characterization of Naturally Occurring Allelic Variants.

    abstract::Genetic polymorphism of the cytochrome P450 (CYP) genes particularly affects CYP2D6 and CYP2C19 to a functionally relevant extent, and it is therefore crucial to elucidate the enzyme kinetic and molecular basis for altered catalytic activity of these allelic variants. This study explored the expression and function of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2728-0

    authors: Dong AN,Pan Y,Palanisamy UD,Yiap BC,Ahemad N,Ong CE

    更新日期:2018-09-01 00:00:00

  • Phylogenetic Analysis and Biological Evaluation of Marine Endophytic Fungi Derived from Red Sea Sponge Hyrtios erectus.

    abstract::Forty-four endophytic fungal isolates obtained from marine sponge, Hyrtios erectus, were evaluated and screened for their hydrolase activities. Most of the isolates were found to be prolific producers of hydrolytic enzymes. Only 11 isolates exhibited maximum cellular contents of lipids, rhamnolipids, and protein in th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2679-x

    authors: El-Gendy MMAA,Yahya SMM,Hamed AR,Soltan MM,El-Bondkly AMA

    更新日期:2018-07-01 00:00:00

  • Purification, characterization, and molecular cloning of a novel antifungal lectin from the roots of Ophioglossum pedunculosum.

    abstract::A novel mannan-specific lectin was isolated from the roots of a traditional Chinese herbal medicine, Ophioglossum pedunculosum through ion-exchange chromatography and gel filtration. With a molecular mass of 19,835.7 Da demonstrated by MALDI-TOF analysis, this novel agglutinin was designated as O. pedunculosum aggluti...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9367-z

    authors: He XM,Ji N,Xiang XC,Luo P,Bao JK

    更新日期:2011-12-01 00:00:00

  • Liquefaction of corn stover and preparation of polyester from the liquefied polyol.

    abstract::This research investigated a novel process to prepare polyester from corn stover through liquefaction and crosslinking processes. First, corn stover was liquefied in organic solvents (90 wt% ethylene glycol and 10 wt% ethylene carbonate) with catalysts at moderate temperature under atmospheric pressure. The effect of ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:130:1:574

    authors: Yu F,Liu Y,Pan X,Lin X,Liu C,Chen P,Ruan R

    更新日期:2006-04-01 00:00:00

  • In Vivo Evaluation of the Toxic Effect of Ethyl Acetate Extracts of Marine Antibiotic Resistance Pseudomonas Species Derived from the Red Sea.

    abstract::Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2553-x

    authors: El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

    更新日期:2018-01-01 00:00:00

  • Statistical analysis and modeling of pelletized cultivation of Mucor circinelloides for microbial lipid accumulation.

    abstract::Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0759-8

    authors: Xia C,Wei W,Hu B

    更新日期:2014-04-01 00:00:00

  • Optimization of the production of thermostable endo-beta-1,4 mannanases from a newly isolated Aspergillus niger gr and Aspergillus flavus gr.

    abstract::The aim of this work was to establish optimal conditions for the maximum production of endo-beta-1,4 mannanases using cheaper sources. Eight thermotolerant fungal strains were isolated from garden soil and compost samples collected in and around the Gulbarga University campus, India. Two strains were selected based on...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8250-z

    authors: Kote NV,Patil AG,Mulimani VH

    更新日期:2009-02-01 00:00:00

  • Characterization of a family B DNA polymerase from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I and its application to PCR.

    abstract::A family B DNA polymerase gene from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I was highly expressed under the control of T7lac promoter of pET-28ARG in Escherichia coli BL21-CodonPlus(DE3)-RIL cells. The produced I. hospitalis (Iho) DNA polymerase was purified by heat treatment followed by HisTrap...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0918-y

    authors: Seo KJ,Cho SS,Ppyun HW,Youn MH,Kim SH,Seo BS,Kwon ST

    更新日期:2014-07-01 00:00:00

  • Discovery of Potent Neuraminidase Inhibitors Using a Combination of Pharmacophore-Based Virtual Screening and Molecular Simulation Approach.

    abstract::Neuraminidase (NA), a surface protein, facilitates the release of nascent virus and thus spreads infection. It has been renowned as a potential drug target for influenza A virus infection. The drugs such as oseltamivir, zanamivir, peramivir, and laninamivir are approved for the treatment of influenza infection. Additi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2625-y

    authors: K R,V S

    更新日期:2018-04-01 00:00:00

  • Opposing effects of external gibberellin and Daminozide on Stevia growth and metabolites.

    abstract::Steviol glycosides (SVglys) and gibberellins are originated from the shared biosynthesis pathway in Stevia (Stevia rebaudiana Bertoni). In this research, two experiments were conducted to study the opposing effects of external gibberellin (GA3) and Daminozide (a gibberellin inhibitor) on Stevia growth and metabolites....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1310-7

    authors: Karimi M,Hashemi J,Ahmadi A,Abbasi A,Pompeiano A,Tavarini S,Guglielminetti L,Angelini LG

    更新日期:2015-01-01 00:00:00

  • Biomethane production in an AnSBBR treating wastewater from biohydrogen process.

    abstract::An anaerobic sequencing batch reactor containing immobilized biomass (AnSBBR) was used to produce biomethane by treating the effluent from another AnSBBR used to produce biohydrogen from glucose- (AR-EPHG) and sucrose-based (AR-EPHS) wastewater. In addition, biomethane was also produced from sucrose-based synthetic wa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1170-1

    authors: Lullio TG,Souza LP,Ratusznei SM,Rodrigues JA,Zaiat M

    更新日期:2014-11-01 00:00:00

  • Determination of cyanide using a microbial sensor.

    abstract::A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02788064

    authors: Nakanishi K,Ikebukuro K,Karube I

    更新日期:1996-08-01 00:00:00

  • In Silico Structure-Based Identification and Validation of Key Residues of Vip3Aa Involving in Lepidopteran Brush Border Receptor Binding.

    abstract::The vegetative insecticidal proteins (VIPs) of Bacillus thuringiensis (Bt) have a broad-spectrum insecticidal activity against Lepidopteran pests and no cross-resistance with the insecticidal crystal protein Cry protein. So there are great potentials for the control of agricultural pests and the resolution of resistan...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s12010-018-2880-6

    authors: Chi B,Li H,Zhang J,Wei P,Gao J,Liu R

    更新日期:2019-04-01 00:00:00

  • Laccase from Coriolus hirsutus as alternate label for enzyme immunoassay. Determination of pesticide 2,4-dichlorophenoxyacetic acid.

    abstract::A new label--laccase from the fungus Coriolus hirsutus--was applied for solid-phase enzyme-linked immunosorbent assays of the pesticide 2,4-dichlorophenoxyacetic acid (2,4-D). Two proposed assays are based on (1) competitive binding of antibody-laccase conjugate with immobilized 2,4-D-protein conjugate and 2,4-D in te...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:76:3:203

    authors: Zherdev AV,Bizova NA,Yaropolov AI,Lyubimova NV,Morozova OV,Dzantiev BB

    更新日期:1999-03-01 00:00:00

  • Microalgal Cultivation and Nutrient Removal from Digested Piggery Wastewater in a Thin-film Flat Plate Photobioreactor.

    abstract::This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.12 day-1 was obta...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2889-x

    authors: Sun ZL,Sun LQ,Chen GZ

    更新日期:2019-04-01 00:00:00

  • Synergistic Anticancer Effect of a Combination of Paclitaxel and 5-Demethylnobiletin Against Lung Cancer Cell Line In Vitro and In Vivo.

    abstract::Lung cancer remains a highly prevalent disease and a leading cause of cancer-related deaths worldwide. Currently, exploring antitumor drugs derived from herbs used in traditional Chinese medicine is increasingly becoming an attractive area of research. Paclitaxel (PTX), a highly effective chemotherapeutic drug, is wid...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2869-1

    authors: Tan KT,Li S,Li YR,Cheng SL,Lin SH,Tung YT

    更新日期:2019-04-01 00:00:00

  • Regeneration of nicotinamide cofactors for use in organic synthesis.

    abstract::The high cost of nicotinamide cofactors requires that they be regenerated in situ when used in preparative enzymatic synthesis. Numerous strategies have been tested for in situ regeneration of reduced and oxidized cofactors. Regeneration of reduced cofactors is relatively straightforward; regeneration of oxidized cofa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/BF02798431

    authors: Chenault HK,Whitesides GM

    更新日期:1987-03-01 00:00:00

  • Simultaneous Wastewater Treatment, Algal Biomass Production and Electricity Generation in Clayware Microbial Carbon Capture Cells.

    abstract::Performance of microbial carbon capture cells (MCCs), having a low-cost clayware separator, was evaluated in terms of wastewater treatment and electricity generation using algae Chlorella pyrenoidosa in MCC-1 and Anabaena ambigua in MCC-2 and without algae in a cathodic chamber of MCC-3. Higher power production was ac...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2485-5

    authors: Jadhav DA,Jain SC,Ghangrekar MM

    更新日期:2017-11-01 00:00:00

  • Functional validation of Phragmites communis glutathione reductase (PhaGR) as an essential enzyme in salt tolerance.

    abstract::Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown. In the present study, we cl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1514-5

    authors: Zhang X,Quan G,Wang J,Han H,Chen S,Guo S,Yin H

    更新日期:2015-04-01 00:00:00

  • Heterologous expression of a Nelumbo nucifera phytochelatin synthase gene enhances cadmium tolerance in Arabidopsis thaliana.

    abstract::Phytochelatin synthase (PCS) is a key enzyme involved in the synthesis of phytochelatins, which are thought to play important roles in heavy metal detoxification. The sacred lotus (Nelumbo nucifera), one of the most popular ornamental species, has been shown to be a potential phytoremediator of heavy metal polluted wa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9461-2

    authors: Liu Z,Gu C,Chen F,Yang D,Wu K,Chen S,Jiang J,Zhang Z

    更新日期:2012-02-01 00:00:00

  • Evaluation of Iminodiacetic Acid (IDA) as an Ionogenic Group for Adsorption of IgG1 Monoclonal Antibodies by Membrane Chromatography.

    abstract::Iminodiacetic acid (IDA) is one of the chelating ligands most frequently employed in immobilized metal-ion affinity chromatography (IMAC) due to its ability to act as electron-pair donor, forming stable complexes with intermediate and borderline Lewis metal ions (electron acceptor). Thus, IDA can also be employed in i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03217-5

    authors: Bresolin ITL,Bresolin IRAP,Bueno SMA

    更新日期:2020-06-01 00:00:00

  • Activity and lifetime of urease immobilized using layer-by-layer nano self-assembly on silicon microchannels.

    abstract::Urease has been immobilized and layered onto the walls of manufactured silicon microchannels. Enzyme immobilization was performed using layer-by-layer nano self-assembly. Alternating layers of oppositely charged polyelectrolytes, with enzyme layers "encased" between them, were deposited onto the walls of the silicon m...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:121:1-3:0085

    authors: Forrest SR,Elmore BB,Palmer JD

    更新日期:2005-04-01 00:00:00

  • Development of DNA-designed avian IgY antibodies for detection of Mycobacterium avium subsp. paratuberculosis heat shock protein 70 (Hsp70) and anti-Hsp70 antibodies in the serum of normal cattle.

    abstract::Mycobacterium avium subsp. paratuberculosis heat shock protein 70 (MAPHsp70) is an immunodominant antigen, which can be used as a subunit vaccine against bovine paratuberculosis. In the present study, we evaluated the immunogenic activities of MAPHsp70 expressed by DNA vaccine in chicken and the use of prepared specif...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9648-1

    authors: Brujeni GN,Gharibi D

    更新日期:2012-05-01 00:00:00

  • The future of coproducts from corn processing.

    abstract::Increased demand for ethanol as a fuel additive has resulted in dramatic growth in ethanol production. Ethanol is produced from corn by either wet milling or dry-grind processing. In wet milling, the corn kernel is fractionated into different components, resulting in several coproducts. Wet-milling plants are capital ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1385/abab:128:1:047

    authors: Rausch KD,Belyea RL

    更新日期:2006-01-01 00:00:00

  • The oxidative stress of Phanerochaete chrysosporium against lead toxicity.

    abstract::Among the technologies for heavy metal remediation, bioremediation technology has gained extensive attention because of its low processing costs and high efficiency. The white-rot fungus Phanerochaete chrysosporium (P. chrysosporium) which has a good tolerance to heavy metals has been widely used in the heavy metal bi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1397-x

    authors: Wan J,Zeng G,Huang D,Huang C,Lai C,Li N,Wei Z,Xu P,He X,Lai M,He Y

    更新日期:2015-02-01 00:00:00

  • Rhamnolipids production by multi-metal-resistant and plant-growth-promoting rhizobacteria.

    abstract::The biosurfactant-producing Pseudomonas aeruginosa A11, with plant-growth-promoting (PGP) and multi-metal-resistant (MMR) features was isolated from the rhizosphere of a wild plant Parthenium hysterophorus. The strain A11 was able to utilize glycerol as a carbon source and produce 4,436.9 mg/L of biosurfactant after 1...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0244-9

    authors: Singh AK,Cameotra SS

    更新日期:2013-07-01 00:00:00