Controlled fed-batch fermentations of dilute-acid hydrolysate in pilot development unit scale.

Abstract:

:Inhibitors formed during wood hydrolysis constitute a major problem in fermenting dilute-acid hydrolysates. By applying a fed-batch technique, the levels of inhibitory compounds may be held low, enabling high ethanol productivity. In this study, a previously developed fed-batch strategy was modified and implemented for use in pilot development unit (PDU) scale. The rate of total gas formation, measured with a mass flow meter, was used as input variable in the control algorithm. The feed rate in the PDU-scale experiments could be properly controlled based on the gas evolution from the reactor. In fed-batch experiments utilizing TMB 3000, an inhibitor-tolerant strain of Saccharomyces cerevisiae, close to 100% of the hexoses in the hydrolysate was converted.

authors

Rudolf A,Galbe M,Lidén G

doi

10.1385/abab:114:1-3:601

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

601-17

eissn

0273-2289

issn

1559-0291

pii

ABAB:114:1-3:601

journal_volume

113-116

pub_type

杂志文章
  • Enhancing the enzymatic hydrolysis of corn stover by an integrated wet-milling and alkali pretreatment.

    abstract::An integrated wet-milling and alkali pretreatment was applied to corn stover prior to enzymatic hydrolysis. The effects of NaOH concentration in the pretreatment on crystalline structure, chemical composition, and reducing-sugar yield of corn stover were investigated, and the mechanism of increasing reducing-sugar yie...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8736-3

    authors: He X,Miao Y,Jiang X,Xu Z,Ouyang P

    更新日期:2010-04-01 00:00:00

  • mga genosensor for early detection of human rheumatic heart disease.

    abstract::The 5' amino-labeled DNA probe complementary to mga gene of Streptococcus pyogenes was immobilized on carboxylated multiwall carbon nanotubes electrode and hybridized with 0.1-100 ng/6 μl single-stranded genomic DNA (ssG-DNA) of S. pyogenes from throat swab of suspected rheumatic heart disease (RHD) patients. Electroc...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0836-z

    authors: Singh S,Kaushal A,Khare S,Kumar A

    更新日期:2014-05-01 00:00:00

  • Magnetic microcapsules for targeted delivery of anticancer drugs.

    abstract::To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes of FM-MMC-mc were made: one with the drug as the core and zinc ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02783752

    authors: Kato T,Nemoto R,Mori H,Abe R,Unno K,Goto A,Murota H,Harada M,Homma M

    更新日期:1984-01-01 00:00:00

  • L-Ribose production from L-arabinose by immobilized recombinant Escherichia coli co-expressing the L-arabinose isomerase and mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans.

    abstract::L-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the L-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The im...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0547-x

    authors: Kim KR,Seo ES,Oh DK

    更新日期:2014-01-01 00:00:00

  • Characterization of Xylanase and Cellulase Produced by a Newly Isolated Aspergillus fumigatus N2 and Its Efficient Saccharification of Barley Straw.

    abstract::Aspergillus fumigatus N2 was isolated from decaying wood. This strain produces extracellular xylanases and cellulases. The highest xylanase (91.9 U/mL) and CMCase (5.61 U/mL) activity was produced when 1% barley straw was used as the carbon source. The optimum pH and temperature for xylanase activity were 6.0 and 65 °...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2344-9

    authors: Lin C,Shen Z,Qin W

    更新日期:2017-06-01 00:00:00

  • Differential protein expression in Phalaenopsis under low temperature.

    abstract::A comparative proteomic analysis was carried out to explore the molecular mechanisms of responses to cold stress in Phalaenopsis after treated by low temperature (13/8 °C day/night) for 15 days. Differentially expressed proteins were examined using two-dimensional electrophoresis (2-DE) and matrix assisted laser desor...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1345-9

    authors: Yuan XY,Liang F,Jiang SH,Wan MF,Ma J,Zhang XY,Cui B

    更新日期:2015-01-01 00:00:00

  • Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.

    abstract::Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide. Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Al...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03345-3

    authors: Xiong J,Cao SL,Zong MH,Lou WY,Wu XL

    更新日期:2020-10-01 00:00:00

  • Solid Flocculation and Emulsifying Activities of the Lipopolysaccharide Produced by Trichosporon mycotoxinivorans CLA2.

    abstract::The objective of this study was to evaluate the capability of a lipopolysaccharide, produced by Trichosporon mycotoxinivorans CLA2 using residue of biodiesel processing, to flocculate two different solids in suspension. In addition, the emulsifying activity and the stability of this lipopolysaccharide in response to p...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2332-0

    authors: Domingues VS,Monteiro AS,Ferreira GF,Santos VL

    更新日期:2017-05-01 00:00:00

  • Studies on the Neuroprotection of Osthole on Glutamate-Induced Apoptotic Cells and an Alzheimer's Disease Mouse Model via Modulation Oxidative Stress.

    abstract::In the present study, the neuroprotection of osthole (OST) was confirmed. In L-glutamic acid (L-Glu)-damaged HT22 cells, a 3-h pre-incubation with OST-enhanced cell viability suppressed the apoptosis rate; inhibited the activities of caspase-3, caspase-8, and caspase-9; reduced the over-accumulation of intracellular r...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03101-2

    authors: Chu Q,Zhu Y,Cao T,Zhang Y,Chang Z,Liu Y,Lu J,Zhang Y

    更新日期:2020-02-01 00:00:00

  • Effect of agitation rate on ethanol production from sugar maple hemicellulosic hydrolysate by Pichia stipitis.

    abstract::Concentrated dilute acid hydrolysate was obtained from hot water extracts of Acer saccharum (sugar maple) and was fermented to ethanol by Pichia stipitis in a 1.3-L-benchtop bioreactor. The conditions under which the highest ethanol yield was achieved were when the air flow rate was set to 100 cm(3) and the agitation ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9285-0

    authors: Shupe AM,Liu S

    更新日期:2012-09-01 00:00:00

  • Sequential anaerobic/aerobic treatment of dye-containing wastewaters: colour and COD removals, and ecotoxicity tests.

    abstract::Colour and COD removals of the azo dyes Congo Red (CR) and Reactive Black 5 (RB5) were individually evaluated in a sequential anaerobic/aerobic treatment system. Additionally, dye toxicity was assessed by using acute ecotoxicity tests with Daphnia magna as the indicator-organism. The anaerobic reactor was operated at ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9493-7

    authors: Silva ME,Firmino PI,Sousa MR,Santos AB

    更新日期:2012-02-01 00:00:00

  • Dehydration of ethanol by facile synthesized glucose-based silica.

    abstract::Bioethanol is considered a potential liquid fuel that can be produced from biomass by fermentation and distillation. Although most of the water is removed by distillation, the purity of ethanol is limited to 95-96 % due to the formation of a low-boiling point, water-ethanol azeotrope. To improve the use of ethanol as ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-0076-z

    authors: Tang B,Bi W,Row KH

    更新日期:2013-02-01 00:00:00

  • Anaerobic reduction of a sulfonated azo dye, Congo Red, by sulfate-reducing bacteria.

    abstract::The capacity for anaerobic decolorization of a sulfonated azo dye, Congo Red, by a strain of a sulfate-reducing bacterium was evaluated. After optimizing the growth rate of the bacteria on a simple carbon source and terminal electron acceptor pair, lactate and sulfate, respectively, the effect of the dye concentration...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:97:3:147

    authors: Diniz PE,Lopes AT,Lino AR,Serralheiro ML

    更新日期:2002-03-01 00:00:00

  • Detoxifying CO2 capture reclaimer waste by anaerobic digestion.

    abstract::The decrease in toxicity of carbon capture reclaimer monoethanolamine (MEA) waste (MEAw) during anaerobic degradation of such waste together with easily degradable organics was investigated. Samples were collected from a bioreactor at steady state with 86 % organic chemical oxygen demand removal at room temperature, w...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0587-2

    authors: Wang S,Hovland J,Brooks S,Bakke R

    更新日期:2014-01-01 00:00:00

  • Purification and characterization of paraoxonase 1 (PON1) from Swiss Black, Holstein, and Montofon bovines.

    abstract::Paraoxonase 1 (PON1: EC 3.1.8.1) is a calcium-dependent enzyme associated with high-density lipoproteins (HDLs) and has a protective effect against oxidation of low-density lipoproteins (LDLs) in mammals. PON1 is the best-studied member of a family of enzymes called serum paraoxonases, or PONs, identified in mammals a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0931-1

    authors: Erzengin M,Demir D,Arslan M,Sinan S

    更新日期:2014-08-01 00:00:00

  • RAPD and ISSR Marker-Based Comparative Evaluation of Genetic Diversity Among Indian Germplasms of Euryale ferox: an Aquatic Food Plant.

    abstract::Euryale ferox Salisbury is an important aquatic food plant cultivated largely in eastern India. E. ferox is a monotypic genus, and breeding programmes have mostly relied on the variability present in the primary gene pool. Knowledge of the genetic structure of the population is limited, and there are very few reports ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2171-z

    authors: Kumar H,Priya P,Singh N,Kumar M,Choudhary BK,Kumar L,Singh IS,Kumar N

    更新日期:2016-12-01 00:00:00

  • Multilayered Nano-Entrapment of Lipase through Organic-Inorganic Hybrid Formation and the Application in Cost-Effective Biodiesel Production.

    abstract::Significant components of cost-effective medium for Magnusiomyces capitatus A4C extracellular lipase (ECL) production were optimized via a five-level factorial design. A simplistic, economical, and green approach was adopted for biomimetic mineralization to prepare multilayered nano-entrapped ECL, which were then appl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03404-9

    authors: Baloch KA,Upaichit A,Cheirsilp B

    更新日期:2021-01-01 00:00:00

  • Preferential lectin binding of cancer cells upon sialic acid treatment under nutrient deprivation.

    abstract::The terminal monosaccharide of glycoconjugates on a eukaryotic cell surface is typically a sialic acid (Neu5Ac). Increased sialylation usually indicates progression and poor prognosis of most carcinomas. Here, we utilize two human mammary epithelial cell lines, HB4A (breast normal cells) and T47D (breast cancer cells)...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0409-6

    authors: Badr HA,Elsayed AI,Ahmed H,Dwek MV,Li CZ,Djansugurova LB

    更新日期:2013-10-01 00:00:00

  • Supplementation of methionine enhanced the ergothioneine accumulation in the Ganoderma neo-japonicum mycelia.

    abstract::The methods for increasing the production of ergothioneine (ERG) were investigated by using the mycelial culture of several mushroom species, primarily Ganoderma neo-japonicum. We first found that ERG was accumulated at the different levels in mycelia and fruiting bodies, respectively, depending on the mushroom specie...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8322-0

    authors: Lee WY,Park EJ,Ahn JK

    更新日期:2009-07-01 00:00:00

  • Diacetyl production mechanism by a strain of Lactococcus lactis spp. lactis bv. diacetylactis. Study of alpha-acetolactic acid extracellular accumulation under anaerobiosis.

    abstract::Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02919393

    authors: Rondags E,Halliday E,Marc I

    更新日期:1998-02-01 00:00:00

  • Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae.

    abstract::A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02922612

    authors: Takuma S,Nakashima N,Tantirungkij M,Kinoshita S,Okada H,Seki T,Yoshida T

    更新日期:1991-04-01 00:00:00

  • Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase.

    abstract::Extracts from different higher plants were screened for the ability to inhibit the growth of Penicillium roqueforti, a major contaminating species in industrial food processing. Oat (Avena sativa) seed extracts exhibited a high degree of antifungal activity and could be used directly on rye bread to prevent the format...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8557-4

    authors: Sørensen HP,Madsen LS,Petersen J,Andersen JT,Hansen AM,Beck HC

    更新日期:2010-03-01 00:00:00

  • A Biotechnological Strategy for Molybdenum Extraction Using Acidithiobacillus ferrooxidans.

    abstract::Biosorption is a potential tool for the extraction of metals from contaminated water and recovery of precious metals, which is a convenient alternative to conventional processes. In the present study, molybdenum recovery by Acidithiobacillus ferrooxidans strain ZT-94 was evaluated. Additionally, the effects of pH init...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03468-7

    authors: Kasra-Kermanshahi R,Tajer-Mohammad-Ghazvini P,Bahrami-Bavani M

    更新日期:2020-11-17 00:00:00

  • Depletion of human lymphocytes from peripheral blood and bone marrow by affinity ligands conjugated to agarose-polyacrolein microsphere beads.

    abstract::Protein-A or goat anti-mouse-Ig (GAMIg) covalently bound to agarose-polyacrolein microsphere beads (APAMB) were employed for the removal of T cells from human peripheral blood leukocytes (PBL) and bone marrow (BM). The cell suspensions were treated with a monoclonal anti-T cell antibody (Leu-1) or monoclonal antilymph...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02798902

    authors: Weiss L,Margel S,Slavin S

    更新日期:1986-10-01 00:00:00

  • Transition metals and organic ligands influence biodegradation of 1,4-dioxane.

    abstract::1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0841-2

    authors: Pornwongthong P,Mulchandani A,Gedalanga PB,Mahendra S

    更新日期:2014-05-01 00:00:00

  • Multiresidue Screening of Veterinary Drugs in Meat, Milk, Egg, and Fish Using Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry.

    abstract::New approaches to veterinary drug screening based on liquid chromatography-mass spectrometry (LC-MS/MS) and time-of-flight mass spectrometry (ToF/MS) are rapid and have high selectivity and sensitivity. In this study, we developed a multiresidue method for screening over 100 veterinary drug residues using ion trap (IT...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2350-y

    authors: Kang J,Park SJ,Park HC,Hossain MA,Kim MA,Son SW,Lim CM,Kim TW,Cho BH

    更新日期:2017-06-01 00:00:00

  • Potential Application and Bactericidal Mechanism of Lactic Acid-Hydrogen Peroxide Consortium.

    abstract::It has been found that lactic acid and hydrogen peroxide (H2O2) displayed co-operatively enhanced killing activity to pathogens. The synergistic effect was investigated with using several microbe species, suggesting that low concentration of lactic acid and H2O2 could kill both Gram-negative and Gram-positive bacteria...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03031-z

    authors: Zhang C,Zhang S,Liu W,Guo T,Gu R,Kong J

    更新日期:2019-11-01 00:00:00

  • Environmental effects on transglutaminase production and cell sporulation in submerged cultivation of Bacillus circulans.

    abstract::In this research, the effects of pH, temperature, and oxygen on growth kinetics of a newly isolated strain of Bacillus circulans from the Amazon and their correlations with transglutaminase (TGase) production and cell sporulation were investigated. Statistical experimental methods were used to optimize these parameter...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8338-5

    authors: de Souza CF,de Matos GS,Flôres SH,Ayub MA

    更新日期:2009-08-01 00:00:00

  • Increased production of thermostable alpha-amylase enzyme by Bacillus sp. TCRDC-25A with maltodextrins.

    abstract::Maltodextrins and hydrolysates of rice and corn flour of varying dextrose equivalents (DE) have been used as a carbon source for alpha-amylase enzyme production by Bacillus sp. TCRDC-25A. The rate and total enzyme production was higher in maltodextrin media than in cornstarch. The enzyme production increased with incr...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02921786

    authors: Bajpai P,Bajpai PK

    更新日期:1991-11-01 00:00:00

  • Improvement of adenosylcobalamin production by metabolic control strategy in Propionibacterium freudenreichii.

    abstract::An efficient metabolic control approach was developed to improve the industrial anaerobic fermentation of adenosylcobalamin (ado-cbl) by Propionibacterium freudenreichii. The effects of 5,6-dimethylbenzimidazole (DMB) on cell growth and ado-cbl biosynthesis were investigated. Subsequently, the results obtained from th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9654-3

    authors: Wang P,Wang Y,Su Z

    更新日期:2012-05-01 00:00:00