Studies on the Neuroprotection of Osthole on Glutamate-Induced Apoptotic Cells and an Alzheimer's Disease Mouse Model via Modulation Oxidative Stress.

abstract：:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was a...

journal_title：Applied biochemistry and biotechnology

authors： Lianghua T,Liming X,Min S,Huaying G

更新日期：2007-08-01 00:00:00

abstract：:Endoglucanase 5 (EG5) has been isolated from the strain of E. coli TG1 harboring recombinant plasmid pCU108, which contains the cel5 gene of C. thermocellum. The enzyme has been produced with 98-fold purification and a final yield of 27% by using subsequent twofold high performance ion-exchange chromatography on Mono ...

journal_title：Applied biochemistry and biotechnology

authors： Mosolova TP,Kalyuzhnyi SV,Varfolomeyev SD,Velikodvorskaya GA

更新日期：1993-07-01 00:00:00

abstract：:Biopolymers and biopreservatives produced by microorganisms play an essential role in food technology. Polyhydroxyalkanoates and bacteriocins produced by bacteria are promising components to safeguard the environment and for food preservation applications. Polyhydroxybutyrate (PHB)-based antimicrobial films were prepa...

journal_title：Applied biochemistry and biotechnology

authors： Narayanan A,Neera,Mallesha,Ramana KV

更新日期：2013-07-01 00:00:00

abstract：:Fungi had become the main resource of polyunsaturated fatty acids, especially linoleic acid. The research studied the effects and mechanism of selenium on polyunsaturated fatty acids of Diasporangium jonesianum. The results showed that selenium could significantly increase the yields of linoleic acid. In contrast, the...

journal_title：Applied biochemistry and biotechnology

authors： Jia Y,Guan XY,Dai CC

更新日期：2014-01-01 00:00:00

abstract：:RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not ...

journal_title：Applied biochemistry and biotechnology

authors： Nübel C,Appel B,Hospach I,Mai M,Krasteva N,Nelles G,Petruschka L,Müller S

更新日期：2016-06-01 00:00:00

abstract：:Carnosol and carnosic acid are polyphenolic compounds found in rosemary and sage with known anti-oxidant, anti-inflammatory, and anti-microbial properties. Here, we addressed the potential use of carnosol and carnosic acid for in vitro bone tissue engineering applications, specifically depending on their cytotoxic eff...

journal_title：Applied biochemistry and biotechnology

authors： Karadas O,Mese G,Ozcivici E

更新日期：2019-06-01 00:00:00

abstract：:The aim of this work is to investigate the binding affinity between the prokaryotic proteins-AHU-IHF proteins (AHU (AHU2, TR3, and AHU6) and IHF (IHF-WT and IHF-βE44A))-and DNAs (DNA, H'-DNA, and H'44A-DNA) by using the steered molecular dynamics (SMD) simulation and the molecular mechanics Poisson-Boltzmann surface a...

journal_title：Applied biochemistry and biotechnology

authors： Nguyen H,Pham T,Nguyen HL,Phan T

更新日期：2018-12-01 00:00:00

abstract：:In this study, an efficient procedure was developed for callus induction and regeneration of kiwifruit (Actinidia deliciosa) using different organs of shoots developed under in vitro conditions. Effects of explants source and media (M(1), 1.0 mg l(-1) BA + 2.0 mg l(-1) 2,4-D-M(2), 1.0 mg l(-1) NAA + 2.0 mg l(-1) 2,4-D...

journal_title：Applied biochemistry and biotechnology

authors： Akbaş F,Işikalan C,Namli S

更新日期：2009-08-01 00:00:00

abstract：:A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed th...

journal_title：Applied biochemistry and biotechnology

authors： Fukuhara Y,Horii S,Matsuno T,Matsumiya Y,Mukai M,Kubo M

更新日期：2013-05-01 00:00:00

abstract：:Among 18 psychrophilic strains isolated near the Antarctic Station, the psychrophilic strain Rhodotorula aurantiaca A19 was selected for its ability of growth and gamma-decalactone production at low temperatures. The effects of temperature, initial pH, and castor oil concentration on the growth and gamma-decalactone p...

journal_title：Applied biochemistry and biotechnology

authors： Alchihab M,Destain J,Aguedo M,Majad L,Ghalfi H,Wathelet JP,Thonart P

更新日期：2009-07-01 00:00:00

abstract：:The synthesis of tricaprylin, tricaprin, trilaurin, and trimyristin in a solvent-free system was conducted by mixing a commercial immobilized lipase with the organic reagents (glycerol and fatty acid) in a 20-mL batch reactor with constant stirring. The effects of temperature, fatty acid/glycerol molar ratio, and enzy...

journal_title：Applied biochemistry and biotechnology

authors： Langone MA,Sant'Anna GL Jr

更新日期：2002-04-01 00:00:00

abstract：:The glpX gene from Francisella tularensis encodes for the class II fructose 1,6-bisphosphatase (FBPaseII) enzyme. The glpX gene has been verified to be essential in F. tularensis, and the inactivation of this gene leads to impaired bacterial growth on gluconeogenic substrates. In the present work, we have complemented...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Wolf NM,Bondoc JMG,Movahedzadeh F

更新日期：2017-12-01 00:00:00

abstract：:Asphaltenes from a crude oil rich in heavy metals (Castilla crude oil) were fractionated and partially characterized. Biocatalytic modifications of these fractionated asphaltenes by three different hemoproteins: chloro-peroxidase (CPO), cytochrome C peroxidase (Cit-C), and lignin peroxidase (LPO) were evaluated in bot...

journal_title：Applied biochemistry and biotechnology

authors： Mogolloń L,Rodríguez R,Larrota W,Ortiz C,Torres R

更新日期：1998-04-01 00:00:00

abstract：:The present study was carried out to understand the effect of cortisol on caspase expression in the C2C12 and 3 T3-L1 cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3 T3-L1 preadipocyte cells. Each cell type was grown independently o...

journal_title：Applied biochemistry and biotechnology

authors： Muthuraman P

更新日期：2014-06-01 00:00:00

abstract：:Internal air circulation affects the temperature field distribution in a gas double-dynamic solid-state fermentation bioreactor (GDSFB). To enhance heat transfer through strengthening internal air circulation in a GDSFB, we put an air distribution plate (ADP) into the bioreactor and studied the effects of forced inter...

journal_title：Applied biochemistry and biotechnology

authors： Chen H,Qin L,Li H

更新日期：2014-02-01 00:00:00

abstract：:Observation of the ultrastructural and intracellular chemical changes of CaCl2-tolerant strains is important both for understanding their adaptation mechanism under high salt stress and for providing theoretical basis of their application in treating high-CaCl2 wastewaters. A novel strain V 430 isolated by us has been...

journal_title：Applied biochemistry and biotechnology

authors： Xin X,Wang Y

更新日期：2007-09-01 00:00:00

abstract：:Current estimates indicate that hepatocarcinoma is the leading cause of death globally. There is interest in utilizing nanomedicine for cancer therapy to overcome side effects of chemo-interventions. Ribavirin, an antiviral nucleoside inhibitor, accumulates inside red blood cells, causing anemia. Its analog, viramidin...

journal_title：Applied biochemistry and biotechnology

authors： Abd-Rabou AA,Bharali DJ,Mousa SA

更新日期：2020-01-01 00:00:00

abstract：:Lung cancer remains a highly prevalent disease and a leading cause of cancer-related deaths worldwide. Currently, exploring antitumor drugs derived from herbs used in traditional Chinese medicine is increasingly becoming an attractive area of research. Paclitaxel (PTX), a highly effective chemotherapeutic drug, is wid...

journal_title：Applied biochemistry and biotechnology

authors： Tan KT,Li S,Li YR,Cheng SL,Lin SH,Tung YT

更新日期：2019-04-01 00:00:00

abstract：:This research aimed at developing and designing a slow and targeted delivery of Amphotericin B (AmB) antibiotic by placing three types of shells containing different ratios of biodegradable and biocompatible polymers poly (D, L-lactide)-co-(glycolide) (PLGA), polyethylene glycol (PEG), and polyvinyl pyrrolidone (PVP) ...

journal_title：Applied biochemistry and biotechnology

authors： Sadat Akhavi S,Moradi Dehaghi S

更新日期：2020-06-01 00:00:00

abstract：:Microalgae farming has been identified as the most eco-sustainable solution for producing biodiesel. However, the operation of full-scale plants is still limited by costs and the utilization of industrial and/or domestic wastes can significantly improve economic profits. Several waste effluents are valuable sources of...

journal_title：Applied biochemistry and biotechnology

authors： Marques SS,Nascimento IA,de Almeida PF,Chinalia FA

更新日期：2013-12-01 00:00:00

abstract：:Microalgae have been proposed as a potential feedstock for biofuel production; however, cell disruption is usually required for collection and utilization of cytoplasmic polysaccharides and lipids. Virus infection might be one approach to disrupt the cell wall. The concentration of yeast extract and presence of KNO3 i...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YS,Labavitch J,VanderGheynst JS

更新日期：2015-05-01 00:00:00

abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...

journal_title：Applied biochemistry and biotechnology

authors： Filiz E,Koc I,Ozyigit II

更新日期：2014-07-01 00:00:00

abstract：:This research describes the development of a multi-analyte lateral-flow immunoassay intended for the simultaneous detection of three anti-protozoan drugs (coccidiostats). These drugs, namely, halofuginone, toltrazuril and diclazuril, are used in the treatment of Eimeria spp. infections in cattle, pigs, chickens and tu...

journal_title：Applied biochemistry and biotechnology

authors： Fitzgerald J,Leonard P,Danaher M,O'Kennedy R

更新日期：2015-05-01 00:00:00

abstract：:The stabilization of olive recombinant hydroperoxide lyases (rHPLs) was investigated using selected chemical additives. Two rHPLs were studied: HPL full-length and HPL with its chloroplast transit peptide deleted (matured HPL). Both olive rHPLs are relatively stable at 4 °C, and enzyme activity can be preserved (about...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Vincenti S,Mariani M,Brunini-Bronzini de Caraffa V,Gambotti C,Desjobert JM,Muselli A,Costa J,Tomi F,Berti L,Maury J

更新日期：2017-07-01 00:00:00

abstract：:Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metallo...

journal_title：Applied biochemistry and biotechnology

authors： Rodriguez C,Nam DH,Kruchowy E,Ge X

更新日期：2017-10-01 00:00:00

abstract：:Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...

journal_title：Applied biochemistry and biotechnology

authors： Bollók M,Réczey K,Zacchi G

更新日期：2000-04-01 00:00:00

abstract：:The mutant strain designated as ART18, obtained from the wild-type strain Clostridium acetobutylicum PW12 treated by atmospheric and room temperature plasma, showed higher solvent tolerance and butanol production than that of the wild-type strain. The production of butanol was 11.3 ± 0.5 g/L, 31 % higher than that of ...

journal_title：Applied biochemistry and biotechnology

authors： Li HG,Luo W,Wang Q,Yu XB

更新日期：2014-04-01 00:00:00

abstract：:The potential of dilute-acid prehydrolysis as a pretreatment method for sugarcane bagasse, rice hulls, peanut shells, and cassava stalks was investigated. The prehydrolysis was performed at 122 degrees C during 20, 40, or 60 min using 2% H(2)SO(4) at a solid-to-liquid ratio of 1:10. Sugar formation increased with incr...

journal_title：Applied biochemistry and biotechnology

authors： Martin C,Alriksson B,Sjöde A,Nilvebrant NO,Jönsson LJ

更新日期：2007-04-01 00:00:00

abstract：:Neoadjuvant chemotherapy (NACT) is known to be beneficial for patients with locally advanced breast cancer. However, there is still no unified standard on the evaluation of NACT. To identify the potential markers related to NACT sensitivity of breast cancer, in the present study, we examined the protein spectrum of br...

journal_title：Applied biochemistry and biotechnology

authors： Zhang K,Yuan K,Wu H,Li Q,Wang Y,Chen S,Zhang L,Gu H,Fu R

更新日期：2012-02-01 00:00:00

abstract：:NADH : FMN oxidoreductase and bacterial luciferase have been efficiently coimmobilized onto Sepharose 4B. This luminescent immobilized enzyme system can be used to assay NADH. The assay is rapid and sensitive with a lower limit of detection of 0.2 pmol/assay tube. The intra-assay precision was 3.5% at 2 × 10(-5) M and...

journal_title：Applied biochemistry and biotechnology

authors： Wienhausen GK,Kricka LJ,Hinkley JE,Deluca M

更新日期：1982-11-01 00:00:00