Abstract:
:Fungi had become the main resource of polyunsaturated fatty acids, especially linoleic acid. The research studied the effects and mechanism of selenium on polyunsaturated fatty acids of Diasporangium jonesianum. The results showed that selenium could significantly increase the yields of linoleic acid. In contrast, the growth and γ-linolenic acid yield of D. jonesianum was decreased under selenium treatments. Δ6-Fatty acid desaturase gene of D. jonesianum was investigated in this research. Sequence analysis indicated that this cDNA sequence encoded 235 amino acids. The conserved region of Δ6-fatty acid desaturase included three conserved histidine-rich domain, hydropathy profile, and was rich in disulfide bonds. This study showed that selenium may in discriminatively substitute S and incorporate selenium-amino acids into the desaturase that the conformation of enzyme active sites was impacted which leaded to the inhibition of the convert of linoleic acid to γ-linolenic acid and the over accumulation of linoleic acid. Selenium might enhance the fatty acid contents of fungi through influencing the desaturase structure.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Jia Y,Guan XY,Dai CCdoi
10.1007/s12010-013-0477-7subject
Has Abstractpub_date
2014-01-01 00:00:00pages
561-9issue
2eissn
0273-2289issn
1559-0291journal_volume
172pub_type
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