Abstract:
:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was about 28 kDa, determined by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. The enzymatic resolution of racemic ibuprofen was carried out by the lipase from P. expansum PED-03, and the conversion reached 46% with excellent enantioselectivity(E > 200), which showed a good application potential in the production of optically pure ibuprofen.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Lianghua T,Liming X,Min S,Huaying Gdoi
10.1007/s12010-007-0043-2subject
Has Abstractpub_date
2007-08-01 00:00:00pages
194-9issue
2eissn
0273-2289issn
1559-0291pii
ABAB:142:2:194journal_volume
142pub_type
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