Abstract:
:A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Sato R,Takeyama H,Tanaka T,Matsunaga Tdoi
10.1385/abab:91-93:1-9:109subject
Has Abstractpub_date
2001-04-01 00:00:00pages
109-16eissn
0273-2289issn
1559-0291journal_volume
91-93pub_type
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