Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system.

Abstract:

:A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.

authors

Sato R,Takeyama H,Tanaka T,Matsunaga T

doi

10.1385/abab:91-93:1-9:109

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

109-16

eissn

0273-2289

issn

1559-0291

journal_volume

91-93

pub_type

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