Optimization of the simultaneous saccharification and fermentation process using thermotolerant yeasts.

abstract：:Sorghum was pretreated by sole ultrasound or supercritical carbon dioxide (scCO2), as well as the method combining both to intensify enzymatic hydrolysis. The effect of the time (1-5 h) and temperature (30-70 °C) on ultrasonic pretreatment was investigated, and the best condition was determined as 5 h and 50 °C with t...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Q,Zhao M,Xu Q,Ren H,Yin J

更新日期：2019-05-01 00:00:00

abstract：:Cancer is one of the killer diseases in humans and needs alternate curative measures despite recent improvement in modern treatment modalities. Oncolytic virotherapy seems to be a promising nonconventional way to treat cancers. Newcastle disease virus (NDV), a poultry virus, is nonpathogenic to human and domestic anim...

journal_title：Applied biochemistry and biotechnology

authors： Kumar R,Tiwari AK,Chaturvedi U,Kumar GR,Sahoo AP,Rajmani RS,Saxena L,Saxena S,Tiwari S,Kumar S

更新日期：2012-08-01 00:00:00

abstract：:The rheological behavior of a sulfated galactan extracted from Halymenia durvillei, a red seaweed collected in the coastal waters of a small island of Madagascar (Nosy-be in Indian Ocean), was investigated in dilute and semi-dilute solutions. In dilute solution with NaCl at 0.3 M, the polysaccharide adopted a coil con...

journal_title：Applied biochemistry and biotechnology

authors： Fenoradosoa TA,Laroche C,Delattre C,Dulong V,Le Cerf D,Picton L,Michaud P

更新日期：2012-07-01 00:00:00

abstract：:Fluorescent proteins from marine organisms represent potential candidates for biosensor development. In this paper, we described the isolation of a native green fluorescent protein from Anemonia sulcata and the cloning and purification of its equivalent as a recombinant protein in Escherichia coli. Furthermore, the sp...

journal_title：Applied biochemistry and biotechnology

authors： Masullo T,Puccio R,Di Pierro M,Tagliavia M,Censi P,Vetri V,Militello V,Cuttitta A,Colombo P

更新日期：2014-02-01 00:00:00

abstract：:A multi-method protocol previously proposed for the extraction of extracellular polymeric substances (EPS) from flocculated sludges was investigated on dense aerobic granules. The protocol combines mechanical disruption by sonication and chemical extraction using the Tween detergent and the cation chelator, EDTA. Poly...

journal_title：Applied biochemistry and biotechnology

authors： Caudan C,Filali A,Lefebvre D,Spérandio M,Girbal-Neuhauser E

更新日期：2012-04-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00

abstract：:Label-free detection technique based on impedance was investigated for aflatoxin M1 (AFM1) and aflatoxin M2 (AFM2) analysis in milk products. The impedance change resulting from antigen-antibody interaction was studied using a two-electrode setup made up of silver (Ag) wire. Processed milk such as drinking yogurt and ...

journal_title：Applied biochemistry and biotechnology

authors： Kanungo L,Bacher G,Bhand S

更新日期：2014-10-01 00:00:00

abstract：:In an effort to increase revenues from a given feedstock, valuable co-products could be extracted prior to biochemical or thermochemical conversion with subcritical water. Although subcritical water shows significant promise in replacing organic solvents as an extraction solvent, compound degradation has been observed...

journal_title：Applied biochemistry and biotechnology

authors： Duan L,Wallace SN,Engelberth A,Lovelady JK,Clausen EC,King JW,Carrier DJ

更新日期：2009-08-01 00:00:00

abstract：:Current estimates indicate that hepatocarcinoma is the leading cause of death globally. There is interest in utilizing nanomedicine for cancer therapy to overcome side effects of chemo-interventions. Ribavirin, an antiviral nucleoside inhibitor, accumulates inside red blood cells, causing anemia. Its analog, viramidin...

journal_title：Applied biochemistry and biotechnology

authors： Abd-Rabou AA,Bharali DJ,Mousa SA

更新日期：2020-01-01 00:00:00

abstract：:Human acidic fibroblast growth factor (haFGF) is a multifunctional protein involved in regulating a wide range of cellular processes. As a potent therapeutic agent, it is highly desirable to produce recombinant haFGF (r-haFGF) at low cost. However, the complex structure and formation of aggregation confines its high-l...

journal_title：Applied biochemistry and biotechnology

authors： Rahman IU,Liu W,Wei Z,Fang L,Zheng X,Huang L,Xu Z

更新日期：2020-08-01 00:00:00

abstract：:Nephelium lappaceum L., commonly known as "rambutan," is a typical tropical tree and is well known for its juicy and sweet fruit which has an exotic flavor. Chemical studies on rambutan have led to the identification of various components such as monoterpene lactones and volatile compounds. Here, a 22.5-kDa trypsin in...

journal_title：Applied biochemistry and biotechnology

authors： Fang EF,Ng TB

更新日期：2015-04-01 00:00:00

abstract：:To investigate the effect of pH and temperature on the cell growth and bacteriocin production of Pediococcus acidilactici PA003, a lactic acid bacterium isolated from traditionally fermented cabbage, the kinetic behaviour of P. acidilactici PA003 was simulated in vitro during laboratory fermentations by making use of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Zhang Y,Liu SN,Han Y,Zhou ZJ

更新日期：2012-03-01 00:00:00

abstract：:The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme. Km and turn over values of the enzyme were 6.25 mg/mL and 5.0 × 102/min, respectively. Effect of different metal i...

journal_title：Applied biochemistry and biotechnology

authors： Mulay YR,Deopurkar RL

更新日期：2018-02-01 00:00:00

abstract：:Strain M(438), deposited as CGMCC3917 and isolated from inoculums of bacterial cellulose (BC) producing strain screened in homemade vinegar and then induced by high hydrostatic pressure treatment (HHP), has strong ability to produce BC more than three times as that of its initial strain. It is the highest yield BC-pro...

journal_title：Applied biochemistry and biotechnology

authors： Ge HJ,Du SK,Lin DH,Zhang JN,Xiang JL,Li ZX

更新日期：2011-12-01 00:00:00

abstract：:Anaerobic sequencing batch reactors containing granular or flocculent biomass have been employed successfully in the treatment of piggery wastewater. However, the studies in which these reactors were employed did not focus specifically on accelerating the hydrolysis step, even though the degradation of this chemical o...

journal_title：Applied biochemistry and biotechnology

authors： de Pinho SC,Fernandes BS,Rodrigues JA,Ratusznei SM,Foresti E,Zaiat M

更新日期：2005-02-01 00:00:00

abstract：:With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...

journal_title：Applied biochemistry and biotechnology

authors： Nidetzky B,Fürlinger M,Gollhofer D,Haug I,Haltrich D,Kulbe KD

更新日期：1997-04-01 00:00:00

abstract：:The adaptive response of the fungus Blakeslea trispora to the oxidative stress induced by butylated hydroxytoluene (BHT) during carotene production in shake flask culture was investigated. The culture response to oxidative stress was studied by measuring the specific activities of catalase (CAT) and superoxide dismuta...

journal_title：Applied biochemistry and biotechnology

authors： Nanou K,Roukas T

更新日期：2010-04-01 00:00:00

abstract：:Genetic polymorphism of the cytochrome P450 (CYP) genes particularly affects CYP2D6 and CYP2C19 to a functionally relevant extent, and it is therefore crucial to elucidate the enzyme kinetic and molecular basis for altered catalytic activity of these allelic variants. This study explored the expression and function of...

journal_title：Applied biochemistry and biotechnology

authors： Dong AN,Pan Y,Palanisamy UD,Yiap BC,Ahemad N,Ong CE

更新日期：2018-09-01 00:00:00

abstract：:The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration...

journal_title：Applied biochemistry and biotechnology

authors： Zhou Y,Domínguez JM,Cao N,Du J,Tsao GT

更新日期：1999-04-01 00:00:00

abstract：:When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of de...

journal_title：Applied biochemistry and biotechnology

authors： Tabbene O,Karkouch I,Slimene IB,Elfeddy N,Cosette P,Mangoni ML,Jouenne T,Limam F

更新日期：2011-05-01 00:00:00

abstract：:This study investigated the effects of flask-to-liquid volume ratio on the growth of Panax ginseng hairy root, transformed by Agrobacterium rhizogenes, in flask cultures and compared the characteristics of various bioreactors for scale-up. The flask-to-liquid volume ratio was optimum at 1.5 mL of air/mL of medium in f...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH,Hwang B,Woo JC

更新日期：2003-04-01 00:00:00

abstract：:The bioaugmentation of petroleum-contaminated soil using Enterobacter cloacae was profiled from the evolution of microbial community, soil dehydrogenase activity, to the degradation of petroleum contaminants. The seeding and proliferation of inoculant and the consequential microbial community were monitored by denatur...

journal_title：Applied biochemistry and biotechnology

authors： Wu Z,Dong H,Zou L,Lu D,Liu Z

更新日期：2011-08-01 00:00:00

abstract：:The use of immobilized enzymes has opened the possibility of large scale utilization of NAD(+)-linked dehydrogenases, but the applications of this technique were limited by the necessity of providing the large amounts of NAD(+) required by its stoichiometric consumption in the reaction. After immobilization of alcohol...

journal_title：Applied biochemistry and biotechnology

authors： Burstein C,Ounissi H,Legoy MD,Gellf G,Thomas D

更新日期：1981-12-01 00:00:00

abstract：:During the aging of yeast culture, Kluyveromyces marxianus undergoes a number of changes in physiology and these changes play a significant role during fermentation. Aged stationary phase cells were found to contain more reactive oxygen species. Additionally, the level of oxidant is counteracted by the antioxidant def...

journal_title：Applied biochemistry and biotechnology

authors： Saini P,Beniwal A,Vij S

更新日期：2017-09-01 00:00:00

abstract：:The terminal monosaccharide of glycoconjugates on a eukaryotic cell surface is typically a sialic acid (Neu5Ac). Increased sialylation usually indicates progression and poor prognosis of most carcinomas. Here, we utilize two human mammary epithelial cell lines, HB4A (breast normal cells) and T47D (breast cancer cells)...

journal_title：Applied biochemistry and biotechnology

authors： Badr HA,Elsayed AI,Ahmed H,Dwek MV,Li CZ,Djansugurova LB

更新日期：2013-10-01 00:00:00

abstract：:The potential market for lactic acid as the feedstock for biodegradable polymers, oxygenated chemicals, and specialty chemicals is significant. L-lactic acid is often the desired enantiomer for such applications. However, stereospecific lactobacilli do not metabolize starch efficiently. In this work, Argonne researche...

journal_title：Applied biochemistry and biotechnology

authors： Tsai SP,Moon SH

更新日期：1998-04-01 00:00:00

abstract：:A simple staged model for the protein foam fractionation process is proposed in this article. This simplified model does not detail the complex foam structure and gas-liquid hydrodynamics in the foam phase but, rather, is built on the conventional theoretical stage concept considering upward bubbles with entrained liq...

journal_title：Applied biochemistry and biotechnology

authors： Du L,Loha V,Tanner RD

更新日期：2000-04-01 00:00:00

abstract：:Iminodiacetic acid (IDA) is one of the chelating ligands most frequently employed in immobilized metal-ion affinity chromatography (IMAC) due to its ability to act as electron-pair donor, forming stable complexes with intermediate and borderline Lewis metal ions (electron acceptor). Thus, IDA can also be employed in i...

journal_title：Applied biochemistry and biotechnology

authors： Bresolin ITL,Bresolin IRAP,Bueno SMA

更新日期：2020-06-01 00:00:00

abstract：:An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprot...

journal_title：Applied biochemistry and biotechnology

authors： Batista FR,Greco KN,Astray RM,Jorge SA,Augusto EF,Pereira CA,Mendonça RZ,Moraes AM

更新日期：2011-01-01 00:00:00

abstract：:Cross-linked enzyme aggregates (CLEAs) are a versatile and effective method for enzyme immobilization, which is exquisitely simple and amenable to rapid optimization. In this study, nature egg white, which is low cost, easily available, and nontoxic, was used as protein feeder to replace traditional protein feeder (bo...

journal_title：Applied biochemistry and biotechnology

authors： Jiang Y,Wang Q,He Y,Zhou L,Gao J

更新日期：2014-03-01 00:00:00