Enhanced Enzymatic Hydrolysis of Sorghum Stalk by Supercritical Carbon Dioxide and Ultrasonic Pretreatment.

abstract：:An improved procedure has been developed for efficient somatic embryogenesis in Anethum graveolens. Green friable embryogenic callus was obtained from hypocotyl segments on medium augmented with 2,4-dichlorophenoxyacetic acid (2,4-D). The highest embryogenic callus induction frequency of 87 % was obtained on Murashige...

journal_title：Applied biochemistry and biotechnology

authors： Dhir R,Shekhawat GS,Alam A

更新日期：2014-08-01 00:00:00

abstract：:A formaldehyde-tolerant bacterium designated as a DM-2 strain was used to biodegrade formaldehyde. The cells, precultivated in the presence of 400 ppm of formaldehyde, were able to degrade formaldehyde in a minimal medium supplemented with up to 400 ppm of formaldehyde in the presence of 3% NaCl. The rate of formaldeh...

journal_title：Applied biochemistry and biotechnology

authors： Yamazaki T,Tsugawa W,Sode K

更新日期：2001-04-01 00:00:00

abstract：:This work describes the influence of two polar lipids, Sn-1/3 and Sn-2 monopalmitin, on the activity of lipase in biphasic systems and in microemulsions. In previous communications, we have shown that Sn-2 monoglycerides can replace Sn-1,3 regiospecific lipases at the oil-water interface, causing a drastically reduced...

journal_title：Applied biochemistry and biotechnology

authors： Reis P,Miller R,Leser M,Watzke H

更新日期：2009-09-01 00:00:00

abstract：:Chitosan/hydroxyapatite composite microparticles were prepared by a solid-in-water-in-oil emulsification cross-linking method. The characteristics and activity in presence of simulated body fluid for 14 and 21 days were investigated. The size distribution, surface morphology, and microstructure of these biomaterials w...

journal_title：Applied biochemistry and biotechnology

authors： Maachou H,Bal K,Bal Y,Chagnes A,Cote G,Aliouche D

更新日期：2012-11-01 00:00:00

abstract：:When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of de...

journal_title：Applied biochemistry and biotechnology

authors： Tabbene O,Karkouch I,Slimene IB,Elfeddy N,Cosette P,Mangoni ML,Jouenne T,Limam F

更新日期：2011-05-01 00:00:00

abstract：:In the fermentation of lignocellulose hydrolysates to ethanol, two major problems are encountered: the fermentation of the pentose sugar xylose, and the presence of microbial inhibitors. Xylose can be directly fermented with yeasts, such as Pachysolen tannophilus, Candida shehatae, and Pichia stipis, or by isomerizati...

journal_title：Applied biochemistry and biotechnology

authors： Hahn-Hägerdal B,Lindén T,Senac T,Skoog K

更新日期：1991-04-01 00:00:00

abstract：:The kinetic parameters of the oxidant action of the combination of enzyme horseradish peroxidase (HRP) with hydrogen peroxide in the degradation of methylene blue dye were investigated. Twenty-one percent of color removal was obtained at pH 5.0 and temperature of 30 °C. Under these conditions, the kinetic parameters K...

journal_title：Applied biochemistry and biotechnology

authors： Pereira AR,da Costa RS,Yokoyama L,Alhadeff EM,Teixeira LA

更新日期：2014-12-01 00:00:00

abstract：:The gene encoding a thermostable β-D-xylosidase (GbtXyl43B) from Geobacillus thermoleovorans IT-08 was cloned in pET30a and expressed in Escherichia coli; additionally, characterization and kinetic analysis of GbtXyl43B were carried out. The gene product was purified to apparent homogeneity showing M r of 72 by sodium...

journal_title：Applied biochemistry and biotechnology

authors： Ratnadewi AA,Fanani M,Kurniasih SD,Sakka M,Wasito EB,Sakka K,Nurachman Z,Puspaningsih NN

更新日期：2013-08-01 00:00:00

abstract：:Nanotechnology is an emerging technology which is an amalgamation of different aspects of science and technology that includes disciplines such as electrical engineering, mechanical engineering, biology, physics, chemistry, and material science. It has potential in the fields of information and communication technolog...

journal_title：Applied biochemistry and biotechnology

authors： Chakraborty M,Jain S,Rani V

更新日期：2011-11-01 00:00:00

abstract：:The metabolic engineering of Chlamydomonas reinhardtii, one of the fastest-growing microalgae, is a potential alternative for enhanced carotenoid productivity. CrtYB (phytoene-β-carotene synthase - PBS) gene from red yeast Xanthophyllomyces dendrorhous encodes for a bifunctional enzyme that harbours both phytoene synt...

journal_title：Applied biochemistry and biotechnology

authors： Rathod JP,Vira C,Lali AM,Prakash G

更新日期：2020-04-01 00:00:00

abstract：:Alkaline phosphatase from hog intestine was immobilized to controlled-pore glass under various conditions. The specific activity of the enzyme was not diminished by immobilization. The influence of temperature and pH on the behavior and the stability of the immobilized enzyme preparations is discussed and compared to ...

journal_title：Applied biochemistry and biotechnology

authors： Pittner F

更新日期：1982-05-01 00:00:00

abstract：:Corn stover has potential as a resource for both fiber and chemical needs if separation strategies can be developed to deal with its heterogeneity. Relative hydrolysis characteristics were assessed for pith (sclerenchyma and parenchyma) and fiber (collenchyma) tissue fractions derived from mechanical separation of cor...

journal_title：Applied biochemistry and biotechnology

authors： Bootsma JA,Shanks BH

更新日期：2005-04-01 00:00:00

abstract：:Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...

journal_title：Applied biochemistry and biotechnology

authors： Wang P,Ma J,Zhang Y,Zhang M,Wu M,Dai Z,Jiang M

更新日期：2016-07-01 00:00:00

abstract：:Kariba weed (Salvinia molesta) was used as biomass feedstock for ethanol production by separate hydrolysis and fermentation (SHF). Monosaccharides from Kariba weed hydrolysate were produced using thermal acid hydrolysis, sonication, and enzymatic saccharification. The optimal conditions for thermal acid hydrolysis of ...

journal_title：Applied biochemistry and biotechnology

authors： Kityo MK,Sunwoo I,Kim SH,Park YR,Jeong GT,Kim SK

更新日期：2020-09-01 00:00:00

abstract：:Further characterization of an aspartyl protease from Mucor bacilliformis with milk-clotting activity was performed. An extinction coefficient, epsilon 278 cm = 1.61 mL/mg/cm, a molecular mass of 35,400 Da and a pI of 5.2 were determined. Proteolytic activity and kinetic parameters were evaluated by using the hexapept...

journal_title：Applied biochemistry and biotechnology

authors： Venera GD,Machalinski C,Zumárraga H,Biscoglio de Jiménez Bonino MJ

更新日期：1997-12-01 00:00:00

abstract：:Microfiltration followed by concentration and diafiltration on ultrafiltration membranes (Biomax-100, Biomax-10 and Ultracel-10) was used to recover extracellular laccase (EC 1.10.3.2) from culture broth of wood-rotting fungus Cerrena unicolor. Feed, permeate, retentate and membrane wash-out solutions were analysed fo...

journal_title：Applied biochemistry and biotechnology

authors： Bryjak J,Rekuć A

更新日期：2010-04-01 00:00:00

abstract：:We engineered Rhizopus chinensis lipase to study its critical amino acid role in catalytic properties. Based on the amino acid sequence and three-dimensional model of the lipase, residues located in its lid hinge region (Met93 and Thr96) were replaced with corresponding amino acid residues (Ile93 and Asn96) found in t...

journal_title：Applied biochemistry and biotechnology

authors： Zhu SS,Li M,Yu X,Xu Y

更新日期：2013-05-01 00:00:00

abstract：:Poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) [poly(HEMA-GMA)] cryogel was synthesized by cryopolymerization technique at frozen temperature. Iminodiacetic acid (IDA) was then attached covalently to the cryogel as a chelating agent. Then, poly(HEMA-GMA)-IDA cryogel was chelated with Ni(II) ions and this n...

journal_title：Applied biochemistry and biotechnology

authors： Uygun M,Akduman B,Akgöl S,Denizli A

更新日期：2013-08-01 00:00:00

abstract：:The separation and purification of biologically functional molecules (e.g., proteins, antibodies, peptides, hormones, low molecular weight biologicals) is of fundamental importance to biotechnology. Affinity separations have become a particularly attractive method for bioseparations due to their high degree of selecti...

journal_title：Applied biochemistry and biotechnology

authors： Dordick JS

更新日期：1991-01-01 00:00:00

abstract：:In this study, the feasibility of introducing redox property to an amphiphilic phospholipid polymer (PMBN) was investigated. The active ester group in the side chain of the polymer was used to react with pyrroloquinoline quinine (PQQ). Redox peaks that corresponded to PQQ redox potentials were observed after the modif...

journal_title：Applied biochemistry and biotechnology

authors： Yu EH,Himuro Y,Takai M,Ishihara K

更新日期：2010-02-01 00:00:00

abstract：:The unique carbohydrate-binding property of lectins makes them invaluable tools in biomedical research. Here, we report the purification, partial primary structure, carbohydrate affinity characterization, crystallization, and preliminary X-ray diffraction analysis of a lactose-specific lectin from Cymbosema roseum see...

journal_title：Applied biochemistry and biotechnology

authors： Rocha BA,Moreno FB,Delatorre P,Souza EP,Marinho ES,Benevides RG,Rustiguel JK,Souza LA,Nagano CS,Debray H,Sampaio AH,de Azevedo WF Jr,Cavada BS

更新日期：2009-03-01 00:00:00

abstract：:Human acidic fibroblast growth factor (haFGF) is a multifunctional protein involved in regulating a wide range of cellular processes. As a potent therapeutic agent, it is highly desirable to produce recombinant haFGF (r-haFGF) at low cost. However, the complex structure and formation of aggregation confines its high-l...

journal_title：Applied biochemistry and biotechnology

authors： Rahman IU,Liu W,Wei Z,Fang L,Zheng X,Huang L,Xu Z

更新日期：2020-08-01 00:00:00

abstract：:Enzymes from thermophilic microorganisms, thermozymes, have unique characteristics such as temperature, chemical, and pH stability. They can be used in several industrial processes, in which they replace mesophilic enzymes or chemicals. Thermozymes are often used when the enzymatic process is compatible with existing ...

journal_title：Applied biochemistry and biotechnology

authors： Bruins ME,Janssen AE,Boom RM

更新日期：2001-02-01 00:00:00

abstract：:Lung cancer remains a highly prevalent disease and a leading cause of cancer-related deaths worldwide. Currently, exploring antitumor drugs derived from herbs used in traditional Chinese medicine is increasingly becoming an attractive area of research. Paclitaxel (PTX), a highly effective chemotherapeutic drug, is wid...

journal_title：Applied biochemistry and biotechnology

authors： Tan KT,Li S,Li YR,Cheng SL,Lin SH,Tung YT

更新日期：2019-04-01 00:00:00

abstract：:The microheterogeneous native amylolytic complex secreted by the isolate A6 of Lactobacillus plantarum revealed a selective enzyme specificity loss when submitted to a limited proteolysis under a suboptimum pH condition. A clear electrophoretic profile change toward just one shorter, more acidic, and equally active po...

journal_title：Applied biochemistry and biotechnology

authors： Florêncio JA,Eiras-Stofella DR,Soccol CR,Raimbault M,Guyot JP,Fontana JD

更新日期：2000-04-01 00:00:00

abstract：:The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, ...

journal_title：Applied biochemistry and biotechnology

authors： Lee S,Lee S,Yoon YJ,Lee J

更新日期：2013-01-01 00:00:00

abstract：:Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of...

journal_title：Applied biochemistry and biotechnology

authors： Rath D,Kumar S,Panda S

更新日期：2019-04-01 00:00:00

abstract：:Gene expression pattern of two important regulatory proteins, salt overly sensitive 2 (SOS2) and plasma membrane protein 3-1 (PMP3-1), involved in ion homeostasis, was analyzed in two salinity-contrasting sunflower (Helianthus annuus L.) lines, Hysun-38 (salt tolerant) and S-278 (moderately salt tolerant). The pattern...

journal_title：Applied biochemistry and biotechnology

authors： Saadia M,Jamil A,Ashraf M,Akram NA

更新日期：2013-06-01 00:00:00

abstract：:Low-cost dried yeasts immobilized on delignified brewers' spent grains for use in wine making and brewing were produced by simple thermal drying techniques. To optimize the thermal drying process, vacuum and air stream conditions were examined. Drying of thin layers of the biocatalysts was performed at low (30-38 degr...

journal_title：Applied biochemistry and biotechnology

authors： Tsaousi K,Koutinas AA,Bekatorou A,Loukatos P

更新日期：2010-09-01 00:00:00

abstract：:The enzyme 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGR) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. This enzyme has three domains: transmembrane, binding, and soluble. In this study, we expressed and puri...

journal_title：Applied biochemistry and biotechnology

authors： Andrade-Pavón D,Cuevas-Hernández RI,Trujillo-Ferrara JG,Hernández-Rodríguez C,Ibarra JA,Villa-Tanaca L

更新日期：2017-08-01 00:00:00