Effective purification of Cerrena unicolor laccase using microfiltration, ultrafiltration and acetone precipitation.

abstract：:Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enzyme properties by i...

journal_title：Applied biochemistry and biotechnology

authors： Wei J,Zhou Y,Xu T,Lu B

更新日期：2010-09-01 00:00:00

abstract：:Titanium-dioxide nanoparticles (TNP) are used in various consumable goods. Evidence has demonstrated the cytotoxicity of TNPs, but exact mechanism is yet to be elucidated. The present study has been aimed at finding out the mechanism of TNP-induced toxicity in biological system. Different doses of anatase-TNPs adminis...

journal_title：Applied biochemistry and biotechnology

authors： Kumar S,Meena R,Paulraj R

更新日期：2016-12-01 00:00:00

abstract：:Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades. For isothermal amplification, DNA polymerases with strand displacement activity are required, and Bst exo- is one of the most commonly used polymerases. However, Bst exo- is able to cause nonspecific DNA amplificat...

journal_title：Applied biochemistry and biotechnology

authors： Garafutdinov RR,Gilvanov AR,Sakhabutdinova AR

更新日期：2020-02-01 00:00:00

abstract：:Fusarium delphinoides (Ascomycota; Nectriaceae) is an indole-3-acetic acid (IAA) producing plant pathogen and a causal agent of wilt in chickpea. The IAA biosynthetic pathway in F. delphinoides strain GPK (FDG) was examined by analyzing metabolic intermediates and by feeding experiments. Gas chromatograph (GC) analysi...

journal_title：Applied biochemistry and biotechnology

authors： Kulkarni GB,Sanjeevkumar S,Kirankumar B,Santoshkumar M,Karegoudar TB

更新日期：2013-02-01 00:00:00

abstract：:Fungi had become the main resource of polyunsaturated fatty acids, especially linoleic acid. The research studied the effects and mechanism of selenium on polyunsaturated fatty acids of Diasporangium jonesianum. The results showed that selenium could significantly increase the yields of linoleic acid. In contrast, the...

journal_title：Applied biochemistry and biotechnology

authors： Jia Y,Guan XY,Dai CC

更新日期：2014-01-01 00:00:00

abstract：:Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for...

journal_title：Applied biochemistry and biotechnology

authors： Kazeem MO,Shah UKM,Baharuddin AS,AbdulRahman NA

更新日期：2017-08-01 00:00:00

abstract：:The current increase in amount of shrimp wastes produced by the shrimp industry has led to the need in finding new methods for shrimp wastes disposal. In this study, an extracellular organic solvent- and oxidant-stable metalloprotease was produced by Bacillus cereus SV1. Maximum protease activity (5,900 U/mL) was obta...

journal_title：Applied biochemistry and biotechnology

authors： Manni L,Jellouli K,Ghorbel-Bellaaj O,Agrebi R,Haddar A,Sellami-Kamoun A,Nasri M

更新日期：2010-04-01 00:00:00

abstract：:Organic wastes are increasingly collected source separated, thus requiring additional treatment or recovery capacities for municipal biowastes, organic industrial wastes, as well as agroindustrial byproducts. In this study, we demonstrate that anaerobic digestion is preferentially suited for highwater- containing liqu...

journal_title：Applied biochemistry and biotechnology

authors： Braun R,Brachtl E,Grasmug M

更新日期：2003-04-01 00:00:00

abstract：:The use of viruses for treatment of cancer overcomes the bottlenecks of chemotherapy and radiotherapy. Several viruses and their proteins have been evaluated for oncolytic effect. The VP3 protein (apoptin) of chicken anemia virus is one such protein with an inherent ability to lyse cancer and transformed cells while l...

journal_title：Applied biochemistry and biotechnology

authors： Singh PK,Tiwari AK,Rajmani RS,Kumar GR,Chaturvedi U,Saxena L,Saxena S,Doley J,Sahoo AP,Santra L,Saxena M,Kumar S,Sharma B

更新日期：2015-05-01 00:00:00

abstract：:Trehalose synthase (TreS) from Meiothermus ruber was co-aggregated with polyethyleneimine (PEI) and precipitated with polyethylene glycol (PEG), followed by cross-linking with glutaraldehyde to obtain TreS-polyethyleneimine cross-linked enzyme aggregates (termed as CLEAs-PEI-PEG). The TreS solution at 0.5 mg mL(-1) pr...

journal_title：Applied biochemistry and biotechnology

authors： Zheng J,Chen Y,Yang L,Li M,Zhang J

更新日期：2014-11-01 00:00:00

abstract：:The effect of thidiazuron (TDZ) has been investigated in shoot multiplication for a simple, efficient, rapid, and commercially applicable regeneration protocol of an important medicinal plant, Vitex trifolia. Multiple shoots were induced in nodal explants obtained from a mature tree on Murashige and Skoog (MS) medium ...

journal_title：Applied biochemistry and biotechnology

authors： Ahmed MR,Anis M

更新日期：2012-11-01 00:00:00

abstract：:Human acidic fibroblast growth factor (haFGF) is a multifunctional protein involved in regulating a wide range of cellular processes. As a potent therapeutic agent, it is highly desirable to produce recombinant haFGF (r-haFGF) at low cost. However, the complex structure and formation of aggregation confines its high-l...

journal_title：Applied biochemistry and biotechnology

authors： Rahman IU,Liu W,Wei Z,Fang L,Zheng X,Huang L,Xu Z

更新日期：2020-08-01 00:00:00

abstract：:The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC(50) value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompeti...

journal_title：Applied biochemistry and biotechnology

authors： Liao WC,Wu WH,Tsai PC,Wang HF,Liu YH,Chan CF

更新日期：2012-01-01 00:00:00

abstract：:A full factorial design leading to 20 sets of sorption runs was conducted to study the influence of four variables (bleaching earth and biomass concentrations, pH, and sorption time) on the iron, nickel, and chromium removal from stainless steel effluent using waste biomass from a beverage industry. Similar factor eff...

journal_title：Applied biochemistry and biotechnology

authors： Dias MA,Rosa CA,Linardi VR,Conte RA,De Castro HF

更新日期：2001-04-01 00:00:00

abstract：:The amount of corn stover and wheat straw that can be sustainably collected in North Carolina was estimated to be 0.64 and 0.16 million dry t/yr, respectively. More than 80% of these crop residues are located in the coastal area. The bioethanol potential from corn stover and wheat straw was estimated to be about 238 m...

journal_title：Applied biochemistry and biotechnology

authors： Shahbazi A,Li Y

更新日期：2006-04-01 00:00:00

abstract：:Colour and COD removals of the azo dyes Congo Red (CR) and Reactive Black 5 (RB5) were individually evaluated in a sequential anaerobic/aerobic treatment system. Additionally, dye toxicity was assessed by using acute ecotoxicity tests with Daphnia magna as the indicator-organism. The anaerobic reactor was operated at ...

journal_title：Applied biochemistry and biotechnology

authors： Silva ME,Firmino PI,Sousa MR,Santos AB

更新日期：2012-02-01 00:00:00

abstract：:Research on biomass pretreatment to enhance enzymatic digestibility has been done for more than decades, but a viable continuous pretreatment method needs to be developed. Extrusion has the potential to be a viable continuous pretreatment method. This study investigated the effect of compression ratio (2:1 and 3:1), s...

journal_title：Applied biochemistry and biotechnology

authors： Karunanithy C,Muthukumarappan K

更新日期：2010-11-01 00:00:00

abstract：:The ability of Xanthomonas campestris to convert glucose and xylose to xanthan and the structure of xanthan derived from the glucose/xylose mixture media are important when the lignocelluloses hydrolysate was used in xanthan production. In this paper, the features related to xanthan fermentation in the glucose/xylose ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Z,Chen H

更新日期：2010-03-01 00:00:00

abstract：:The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased su...

journal_title：Applied biochemistry and biotechnology

authors： Sousa CA,Hanselaer S,Soares EV

更新日期：2015-01-01 00:00:00

abstract：:A lactate oxidase was purified about 36-fold from a newly screened strain KY6 of gram negative bacterium from soil to yield a homogeneous protein. The native enzyme had a molecular mass of 204 kDa measured by Sephadex G-200 and that of subunit on the SDS-PAGE was found to be 45 kDa. The enzyme was optimally active at ...

journal_title：Applied biochemistry and biotechnology

authors： Xu P,Yano T,Yamamoto K,Suzuki H,Kumagai H

更新日期：1996-03-01 00:00:00

abstract：:Vegetable and fruit waste (VFW) is becoming a heavy burden of municipal waste disposal because of its huge amount, but it is a potentially valuable resource that can be developed into high value products such as methane. Conventional anaerobic digestion processes are not suitable for solving the problem of easy acidif...

journal_title：Applied biochemistry and biotechnology

authors： Liu WY,Liao B

更新日期：2019-05-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) produced by a biofilm-producing bacterium Acinetobacter junii BB1A were characterized. Purified EPS on analysis revealed neutral sugar (73.21 %), uronic acid (10.12 %), amino sugars (0.23 %), α- amino acids (11.13 %), and aromatic amino acids (1.23 %). Infrared spectrometry rev...

journal_title：Applied biochemistry and biotechnology

authors： Yadav KK,Mandal AK,Sen IK,Chakraborti S,Islam SS,Chakraborty R

更新日期：2012-11-01 00:00:00

abstract：:Lysozyme was immobilized by two different methods in two different ways in order to obtain a preparation with an activity as high as possible toward a macromolecular substrate. The enzyme was bound as a Schiff base to a silicate carrier by using oxidized dextrans of different lengths as spacer and also was bound to co...

journal_title：Applied biochemistry and biotechnology

authors： Moser I,Dworsky P,Pittner F

更新日期：1988-12-01 00:00:00

abstract：:In this article, two theories are unified to investigate the effect of hydrodynamics on a specific bioprocess: the network-of-zones (NOZ) hydrodynamic structured modeling approach (developed by several researchers but applied to only a few bioprocesses) and the effectiveness factor eta approach. Two process scales wer...

journal_title：Applied biochemistry and biotechnology

authors： Delvigne F,El Mejdoub T,Destain J,Delroisse JM,Vandenbol M,Haubruge E,Thonart P

更新日期：2005-04-01 00:00:00

abstract：:Operational practice of high-rate anaerobic bioreactors such as upflow anaerobic sludge bed (UASB) reactors is generally based on maximization of the biomass concentration and, in the case of more than one reactor compartment, operation in parallel. In this article, a modeling approach is used to postulate that the tr...

journal_title：Applied biochemistry and biotechnology

authors： Kleerebezem R

更新日期：2003-04-01 00:00:00

abstract：:More than two decades ago, a group of research engineers and applied scientists with interest in energy applications of bioprocessing initiated an annual symposium series ultimately entitled "Biotechnology for Fuels and Chemicals." The Department of Energy, several of the national laboratories, and various industrial ...

journal_title：Applied biochemistry and biotechnology

authors： Scott CD

更新日期：2004-04-01 00:00:00

abstract：:The use of immobilized enzymes has opened the possibility of large scale utilization of NAD(+)-linked dehydrogenases, but the applications of this technique were limited by the necessity of providing the large amounts of NAD(+) required by its stoichiometric consumption in the reaction. After immobilization of alcohol...

journal_title：Applied biochemistry and biotechnology

authors： Burstein C,Ounissi H,Legoy MD,Gellf G,Thomas D

更新日期：1981-12-01 00:00:00

abstract：:Pretreatment based on aqueous ammonia was investigated under two different modes of operation: soaking in aqueous ammonia and ammonia recycle percolation. These processes were applied to three different feedstocks with varied composition: corn stover, high lignin (HL), and low lignin (LL) hybrid poplars. One of the im...

journal_title：Applied biochemistry and biotechnology

authors： Gupta R,Kim TH,Lee YY

更新日期：2008-03-01 00:00:00

abstract：:Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

journal_title：Applied biochemistry and biotechnology

authors： El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

更新日期：2018-01-01 00:00:00

abstract：:Over the last two decades many strategies have been planned to design specific drugs for rare diseases to target their action at the DNA level. Advancements in our understanding of the interactions of small nonpeptide molecules with DNA have opened the doors for "rational" drug design. Special methods have now been de...

journal_title：Applied biochemistry and biotechnology

authors： Prabhakar P,Kayastha AM

更新日期：1994-04-01 00:00:00