Thermozymes and their applications: a review of recent literature and patents.

Abstract:

:Enzymes from thermophilic microorganisms, thermozymes, have unique characteristics such as temperature, chemical, and pH stability. They can be used in several industrial processes, in which they replace mesophilic enzymes or chemicals. Thermozymes are often used when the enzymatic process is compatible with existing (high-temperature) process conditions. The main advantages of performing processes at higher temperatures are reduced risk of microbial contamination, lower viscosity, improved transfer rates, and improved solubility of substrates. However, cofactors, substrates, or products might be unstable or other side reactions may occur. Recent developments show that thermophiles are a good source of novel catalysts that are of great industrial interest. Thermostable polymer-degrading enzymes such as amylases, pullulanases, xylanases, proteases, and cellulases are expected to play an important role in food, chemical, pharmaceutical, paper, pulp, and waste-treatment industries. Considerable research efforts have been made to better understand the stability of thermozymes. There are no major conformational differences with mesophilic enzymes, and a small number of extra salt bridges, hydrophobic interactions, or hydrogen bounds seem to confer the extra degree of stabilization. Currently, overexpression of thermozymes in standard Escherichia coli allows the production of much larger quantities of enzymes, which are easy to purify by heat treatment. With wider availability and lower cost, thermophilic enzymes will see more application in industry.

authors

Bruins ME,Janssen AE,Boom RM

doi

10.1385/abab:90:2:155

subject

Has Abstract

pub_date

2001-02-01 00:00:00

pages

155-86

issue

2

eissn

0273-2289

issn

1559-0291

pii

ABAB:90:2:155

journal_volume

90

pub_type

杂志文章,评审
  • Functional Characterization of a Novel Marine Microbial Esterase and its Utilization in the Enantioselective Preparation of (R)-Methyl 2-Chloropropionate.

    abstract::Chiral 2-chloropropanoic acids and their ester derivatives are crucial intermediates in the synthesis of many chemicals, especially herbicides. The enzymatic synthesis of chiral 2-chloropropanoic acids and their ester derivatives by esterases was not easily achieved, because the structural difference between the two e...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2094-8

    authors: Cao Y,Deng D,Sun A,Zhang Y,Hu Y

    更新日期:2016-09-01 00:00:00

  • ASBR applied to the treatment of biodiesel production effluent: effect of organic load and fill time on performance and methane production.

    abstract::The effect of organic matter and fill time on anaerobic sequencing batch reactor (5 L, 30 °C, 8-h cycles, 50 rpm) efficiency has been analyzed. Organic matter was increased by the influent concentration. Fill times investigated were in the batch mode and fed-batch followed by batch. In the batch mode organic matter re...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9009-x

    authors: Selma VC,Cotrim LH,Rodrigues JA,Ratusznei SM,Zaiat M,Foresti E

    更新日期:2010-12-01 00:00:00

  • Purification and partial characterization of lignin peroxidase from Acinetobacter calcoaceticus NCIM 2890 and its application in decolorization of textile dyes.

    abstract::Lignin peroxidase was purified (72-fold) from Acinetobacter calcoaceticus NCIM 2890. The purified lignin peroxidase (55-65 kDa) showed dimeric nature. The maximum enzyme activity was observed at pH 1.0, between a broad temperature range of 50 and 70 degrees C, at H2O2 concentration (40 mM) and the substrate concentrat...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8258-4

    authors: Ghodake GS,Kalme SD,Jadhav JP,Govindwar SP

    更新日期:2009-01-01 00:00:00

  • Pilot-scale bioremediation of PAH-contaminated soils.

    abstract::The Institute of Gas Technology (IGT) conducted a pilot-scale study at a former manufactured gas plant (MGP) site in New Jersey. The objective of the study was to determine the effectiveness of an innovative chemical/biological treatment process (MGP-REM process) to remediate soils contaminated with polynuclear aromat...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920473

    authors: Pradhan SP,Paterek JR,Liu BY,Conrad JR,Srivastava VJ

    更新日期:1997-04-01 00:00:00

  • Kinetics of ergothioneine inhibition of mushroom tyrosinase.

    abstract::The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC(50) value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompeti...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9421-x

    authors: Liao WC,Wu WH,Tsai PC,Wang HF,Liu YH,Chan CF

    更新日期:2012-01-01 00:00:00

  • Expression of recombinant human nerve growth factor beta in milk of goats by recombinant replication-defective adenovirus.

    abstract::For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expressi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8346-5

    authors: Xiao B,Li Q,Feng B,Han Z,Gao D,Zhao R,Li J,Li K,Zhi X,Yang H,Liu Z

    更新日期:2009-06-01 00:00:00

  • Phytodegradation potential of Erythrina crista-galli L., Fabaceae, in petroleum-contaminated soil.

    abstract::This work aimed at investigating both the tolerance and the phytodegradation potential of Erythrina crista-galli L. in petroleum-contaminated soil. It consisted in analyzing E. crista-galli germination, surviving, growth, and development when cultivated at different contaminant concentrations and pollutant degradation...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8531-1

    authors: de Farias V,Maranho LT,de Vasconcelos EC,da Silva Carvalho Filho MA,Lacerda LG,Azevedo JA,Pandey A,Soccol CR

    更新日期:2009-04-01 00:00:00

  • Enriched microbial community in bioaugmentation of petroleum-contaminated soil in the presence of wheat straw.

    abstract::The bioaugmentation of petroleum-contaminated soil using Enterobacter cloacae was profiled from the evolution of microbial community, soil dehydrogenase activity, to the degradation of petroleum contaminants. The seeding and proliferation of inoculant and the consequential microbial community were monitored by denatur...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9195-1

    authors: Wu Z,Dong H,Zou L,Lu D,Liu Z

    更新日期:2011-08-01 00:00:00

  • Comparative study of SOS2 and a novel PMP3-1 gene expression in two sunflower (Helianthus annuus L.) lines differing in salt tolerance.

    abstract::Gene expression pattern of two important regulatory proteins, salt overly sensitive 2 (SOS2) and plasma membrane protein 3-1 (PMP3-1), involved in ion homeostasis, was analyzed in two salinity-contrasting sunflower (Helianthus annuus L.) lines, Hysun-38 (salt tolerant) and S-278 (moderately salt tolerant). The pattern...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0240-0

    authors: Saadia M,Jamil A,Ashraf M,Akram NA

    更新日期:2013-06-01 00:00:00

  • Optimization of biodiesel production from castor oil using response surface methodology.

    abstract::The short supply of edible vegetable oils is the limiting factor in the progression of biodiesel technology; thus, in this study, we applied response surface methodology in order to optimize the reaction factors for biodiesel synthesis from inedible castor oil. Specifically, we evaluated the effects of multiple parame...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8468-9

    authors: Jeong GT,Park DH

    更新日期:2009-05-01 00:00:00

  • Intracellular fate of hydrocarbons: possible existence of specific compartments for their biodegradation.

    abstract::In previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the YR-1 strain of Mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sAO) activity. In the present work enzym...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:121:1-3:0205

    authors: Silva-Jiménez H,Zazueta-Sandoval R

    更新日期:2005-04-01 00:00:00

  • Effect of cortisol on caspases in the co-cultured C2C12 and 3 T3-L1 cells.

    abstract::The present study was carried out to understand the effect of cortisol on caspase expression in the C2C12 and 3 T3-L1 cells under co-culture system. Cells were co-cultured by using transwell inserts with a 0.4-μm porous membrane to separate C2C12 and 3 T3-L1 preadipocyte cells. Each cell type was grown independently o...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0909-z

    authors: Muthuraman P

    更新日期:2014-06-01 00:00:00

  • Proteomics and detection of uromodulin in first-time renal calculi patients and recurrent renal calculi patients.

    abstract::Renal calculi disease or known as kidney stone disease is the most common urological disorder in both men and women, although it is more prevalent in men. The lifetime chance for an individual to develop renal calculi is approximately 10% whereas the risk of recurrence in a 10-year period is 74%. Therefore, a diagnost...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8503-x

    authors: Wai-Hoe L,Wing-Seng L,Ismail Z,Lay-Harn G

    更新日期:2009-10-01 00:00:00

  • A biocompatible needle-type glucose sensor based on platinum-electroplated carbon electrode.

    abstract::A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilize...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02921780

    authors: Chen CY,Tamiya E,Ishihara K,Kosugi Y,Su YC,Nakabayashi N,Karube I

    更新日期:1992-09-01 00:00:00

  • Parameter estimation for simultaneous saccharification and fermentation of food waste into ethanol using Matlab Simulink.

    abstract::The increase in waste disposal and energy costs has provided an incentive to convert carbohydrate-rich food waste streams into fuel. For example, dining halls and restaurants discard foods that require tipping fees for removal. An effective use of food waste may be the enzymatic hydrolysis of the waste to simple sugar...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-8045-7

    authors: Davis RA

    更新日期:2008-03-01 00:00:00

  • Functional annotation of Fibrobacter succinogenes S85 carbohydrate active enzymes.

    abstract::Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009. Using new expression tools developed at...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9070-5

    authors: Brumm P,Mead D,Boyum J,Drinkwater C,Deneke J,Gowda K,Stevenson D,Weimer P

    更新日期:2011-03-01 00:00:00

  • Recombination system based on cre alpha complementation and leucine zipper fusions.

    abstract::In a previous study, a recombination system based on the alpha complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (beta) and the transduction of the N-terminal (alpha) cre fragments to cells as a purifie...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8409-7

    authors: Seidi A,Mie M,Kobatake E

    更新日期:2009-08-01 00:00:00

  • Evaluation of wet oxidation pretreatment for enzymatic hydrolysis of softwood.

    abstract::The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid an...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:117:1:01

    authors: Palonen H,Thomsen AB,Tenkanen M,Schmidt AS,Viikari L

    更新日期:2004-04-01 00:00:00

  • Combining mixing regimes for optimized anaerobic wastewater treatment.

    abstract::Operational practice of high-rate anaerobic bioreactors such as upflow anaerobic sludge bed (UASB) reactors is generally based on maximization of the biomass concentration and, in the case of more than one reactor compartment, operation in parallel. In this article, a modeling approach is used to postulate that the tr...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:109:1-3:3

    authors: Kleerebezem R

    更新日期:2003-04-01 00:00:00

  • Molecular Characterization of Xylobiose- and Xylopentaose-Producing β-1,4-Endoxylanase SCO5931 from Streptomyces coelicolor A3(2).

    abstract::Streptomyces coelicolor A3(2) sco5931 gene was predicted to encode a putative xylanase A, a 477 amino acid protein belonging to glycoside hydrolase family 10. The entire sco5931 coding region was cloned and overexpressed in Streptomyces lividans TK24. Mature SCO5931 protein comprising 436 amino acids (47 kDa) was puri...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2103-y

    authors: Enkhbaatar B,Lee CR,Hong YS,Hong SK

    更新日期:2016-09-01 00:00:00

  • Design and evaluation of an optimal controller for simultaneous saccharification and fermentation process.

    abstract::Ethanol from corn is produced using dry grind corn process in which simultaneous saccharification and fermentation (SSF) is one of the most critical unit operations. In this work an optimal controller based on a previously validated SSF model was developed by formulating the SSF process as a Bolza problem and using gr...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9406-9

    authors: Murthy GS,Johnston DB,Rausch KD,Tumbleson ME,Singh V

    更新日期:2012-01-01 00:00:00

  • Fungal Pretreatment of Sweet Sorghum Bagasse with Combined CuSO4-Gallic Acid Supplement for Improvement in Lignin Degradation, Selectivity, and Enzymatic Saccharification.

    abstract::Sweet sorghum (Sorghum sp.) has high biomass yield. Hydrolysis of lignocellulosic sweet sorghum bagasse (SSB) to fermentable sugar could be useful for manufacture of biofuel or other fermentation products. Pretreatment of lignocellulosic biomass to degrade lignin before enzymatic hydrolysis is a key step. Fungal pretr...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2439-y

    authors: Mishra V,Jana AK

    更新日期:2017-09-01 00:00:00

  • Xylanase recovery by ethanol and Na2SO4 precipitation.

    abstract::Xylans are the major components of the hemicellulosic fraction of lignocellulosic biomass and their hydrolysis can be obtained using xylanases from Penicillium janthinellum. In this work, sugarcane bagasse hemicellulosic hydrolysate was used as the substrate for producing xylanase. The precipitation of these enzymes w...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920177

    authors: Cortez EV,Pessoa A Jr,Assis AN

    更新日期:1998-04-01 00:00:00

  • In silico and experimental characterization of chimeric Bacillus thermocatenulatus lipase with the complete conserved pentapeptide of Candida rugosa lipase.

    abstract::Lipases are one of the highest value commercial enzymes as they have broad applications in detergent, food, pharmaceutical, and dairy industries. To provide chimeric Bacillus thermocatenulatus lipase (BTL2), the completely conserved pentapeptide (¹¹²Ala-His-Ser-Gln-Gly¹¹⁶) was replaced with similar sequences (²⁰⁷Gly-G...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-0014-0

    authors: Hosseini M,Karkhane AA,Yakhchali B,Shamsara M,Aminzadeh S,Morshedi D,Haghbeen K,Torktaz I,Karimi E,Safari Z

    更新日期:2013-02-01 00:00:00

  • Distribution of hydrocarbon-degrading bacteria in the soil environment and their contribution to bioremediation.

    abstract::A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0170-x

    authors: Fukuhara Y,Horii S,Matsuno T,Matsumiya Y,Mukai M,Kubo M

    更新日期:2013-05-01 00:00:00

  • Biosynthesis of Alanyl-Histidine Dipeptide Catalyzed by Papain Immobilized on Magnetic Nanocrystalline Cellulose in Deep Eutectic Solvents.

    abstract::Papain (PA) immobilized onto magnetic nanocrystalline cellulose (PA@MNCC) was successfully fabricated and adopted as an efficient biocatalyst for the synthesis of N-(benzyloxycarbonyl)-alanyl-histidine (Z-Ala-His) dipeptide. Introducing deep eutectic solvents (DESs) as reaction media promoted the synthesis of the Z-Al...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03345-3

    authors: Xiong J,Cao SL,Zong MH,Lou WY,Wu XL

    更新日期:2020-10-01 00:00:00

  • Enhancement of L-asparaginase production by isolated Bacillus circulans (MTCC 8574) using response surface methodology.

    abstract::L-asparaginase production was optimized using isolated Bacillus circulans (MTCC 8574) under solid-state fermentation (SSF) using locally available agricultural waste materials. Among different agricultural materials (red gram husk, bengal gram husk, coconut, and groundnut cake), red gram husk gave the maximum enzyme p...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8438-2

    authors: Hymavathi M,Sathish T,Subba Rao Ch,Prakasham RS

    更新日期:2009-10-01 00:00:00

  • Potential use of cutinase in enzymatic scouring of cotton fiber cuticle.

    abstract::The present study characterized the ability of a bacterial cutinase to improve the wettability of raw cotton fabrics by specific hydrolysis of the cutin structure of the cuticle. The effect of cutinase was studied alone and in coreaction with pectin lyase. The changes in both the fabric and the reaction fluid were mea...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:102-103:1-6:277

    authors: Degani O,Gepstein S,Dosoretz CG

    更新日期:2002-07-01 00:00:00

  • The effect of trifluoroethanol on tyrosinase activity and conformation: inhibition kinetics and computational simulations.

    abstract::We studied the inhibitory effects of trifluoroethanol (TFE) on the activity and conformation of tyrosinase. TFE increased the degree of secondary structure of tyrosinase, which directly resulted in enzyme inactivation. A reciprocal study showed that TFE inhibited tyrosinase in a slope-parabolic mixed-type inhibition m...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8730-9

    authors: Lü ZR,Shi L,Wang J,Park D,Bhak J,Yang JM,Park YD,Zhou HW,Zou F

    更新日期:2010-04-01 00:00:00

  • Observation of geometric structure of collagen molecules by atomic force microscopy.

    abstract::Atomic force microscopy was used to study the geometric structure of collagen fibrils and molecules of rat calcanean tendon tissues. The authors found that the diameter of the fibrils ranged from 124 to 170 nm, and their geometric form suggested a helical winding with spectral period from 59.4 to 61.7 nm, close to the...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02919391

    authors: Baranauskas V,Vidal BC,Parizotto NA

    更新日期:1998-02-01 00:00:00