Callus induction and plant regeneration from different explants of Actinidia deliciosa.

Abstract:

:In this study, an efficient procedure was developed for callus induction and regeneration of kiwifruit (Actinidia deliciosa) using different organs of shoots developed under in vitro conditions. Effects of explants source and media (M(1), 1.0 mg l(-1) BA + 2.0 mg l(-1) 2,4-D-M(2), 1.0 mg l(-1) NAA + 2.0 mg l(-1) 2,4-D) on initiation of callus were examined in order to obtain callus for organogenesis. The best callus for plant regeneration was obtained from leaf explants on Murashige and Skoog's medium (MS) supplemented with M(2). Formation of callus from leaf of kiwifruit (A. deliciosa) was cultured in MS medium containing different concentration of N(6)-benzylaminopurin (BA; 0.0, 1.0, 2.0, 4.0, 6.0, 8.0 mg l(-1)) for callus proliferation and plant regeneration. Although the first shoot formation was appeared in medium containing 6.0 and 8.0 mg l(-1) BA, the best shoots formation was obtained in medium with 4.0 mg l(-1) BA.

authors

Akbaş F,Işikalan C,Namli S

doi

10.1007/s12010-008-8401-2

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

470-5

issue

2

eissn

0273-2289

issn

1559-0291

journal_volume

158

pub_type

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