Characterization of a family B DNA polymerase from the hyperthermophilic crenarchaeon Ignicoccus hospitalis KIN4/I and its application to PCR.

abstract：:Convenient expression systems for efficient heterologous production of different laccases are needed for their characterization and application. The laccase cDNAs lcc1 and lcc2 from Trametes versicolor were expressed in Pichia pastoris and Aspergillus niger under control of their respective glyceraldehyde-3-phosphate ...

journal_title：Applied biochemistry and biotechnology

authors： Bohlin C,Jönsson LJ,Roth R,van Zyl WH

更新日期：2006-04-01 00:00:00

abstract：:A new process for enzymatic synthesis of biodiesel at high water content (10-20%) with 96% conversion by lipase from Candida sp. 99-125 was studied. The lipase, a no-position-specific lipase, was immobilized by a cheap cotton membrane and the membrane-immobilized lipase could be used at least six times with high conve...

journal_title：Applied biochemistry and biotechnology

authors： Tan T,Nie K,Wang F

更新日期：2006-02-01 00:00:00

abstract：:The recovery of alpha-amylase from the crude enzyme preparation by the reversed micellar liquid-liquid extraction was investigated. The reversed micellar solution was formed by dissolving a cationic surfactant Aliquat 336 in six different alkanes (cyclohexane, n-hexane, isooctane, n-octane, n-decane, and n-dodecane) r...

journal_title：Applied biochemistry and biotechnology

authors： Chang QL,Chen JY

更新日期：1997-02-01 00:00:00

abstract：:Acute lymphoblastic leukaemia (ALL) is one of the leading types of malignant disorder seen in children. Viral infections, genetic factors and exposure to chemical carcinogens are some of the factors responsible for causing ALL. Treatment strategies followed for curing ALL include chemotherapy or radiation therapy, whe...

journal_title：Applied biochemistry and biotechnology

authors： Ramya LN,Doble M,Rekha VP,Pulicherla KK

更新日期：2012-08-01 00:00:00

abstract：:During glycerol fed-batch fermentation, the process could be divided into multiple equal subintervals, and the feed operation was performed in pulse form at the start of each subinterval. Based on the macrokinetic models, the multipulse feed strategy for both glucose and corn steep slurry was determined by a general n...

journal_title：Applied biochemistry and biotechnology

authors： Xie D,Liu D,Zhu H,Liu T

更新日期：2001-08-01 00:00:00

abstract：:A new bioprocess for production of sorbitol and gluconic acid from two low-cost feedstocks, inulin and cassava starch, using a commercially available enzyme was proposed in this study. The commercial glucoamylase GA-L NEW from Genencor was found to demonstrate a high inulinase activity for hydrolysis of inulin into fr...

journal_title：Applied biochemistry and biotechnology

authors： An K,Hu F,Bao J

更新日期：2013-12-01 00:00:00

abstract：:The currently used multistep chemical synthesis for making surfaces antimicrobial by attaching to them hydrophobic polycations is replaced herein by an aerosol-assisted plasma deposition procedure. To this end, N,N-hexyl,methyl-PEI (HMPEI) is directly plasma-coated onto a glass surface. The resultant immobilized HMPEI...

journal_title：Applied biochemistry and biotechnology

authors： Liu H,Kim Y,Mello K,Lovaasen J,Shah A,Rice N,Yim JH,Pappas D,Klibanov AM

更新日期：2014-02-01 00:00:00

abstract：:The metabolism of residual glycerol from biodiesel synthesis by Klebsiella pneumoniae BLh-1 was investigated in this study. Batch and fed-batch cultivations were performed in bioreactors under anaerobic and oxygen limitation conditions. Results of batch cultivations showed that the main product was 1,3-propanediol (1,...

journal_title：Applied biochemistry and biotechnology

authors： Rossi DM,de Souza EA,Ayub MA

更新日期：2013-03-01 00:00:00

abstract：:Lignin peroxidase was purified (72-fold) from Acinetobacter calcoaceticus NCIM 2890. The purified lignin peroxidase (55-65 kDa) showed dimeric nature. The maximum enzyme activity was observed at pH 1.0, between a broad temperature range of 50 and 70 degrees C, at H2O2 concentration (40 mM) and the substrate concentrat...

journal_title：Applied biochemistry and biotechnology

authors： Ghodake GS,Kalme SD,Jadhav JP,Govindwar SP

更新日期：2009-01-01 00:00:00

abstract：:Glial cell line-derived neurotrophic factor (GDNF) is a type of neurotrophic factor with significant potential in treatment of Parkinson's disease. Combining gene targeting of animal somatic cells with nuclear transfer technique has provided a powerful method to produce transgenic animal mammary gland bioreactor. The ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang X,Wu Y,Luo F,Su H,Bai Y,Hou Y,Yu B

更新日期：2009-12-01 00:00:00

abstract：:Aroma volatiles in Basmati-370, Ambemohar-157 (non-basmati scented), and IR-64 (non-scented) rice cultivars were qualitatively and quantitatively analyzed at vegetative and maturity stages to study their differential accumulation using headspace solid-phase microextraction, followed by gas chromatography mass spectrom...

journal_title：Applied biochemistry and biotechnology

authors： Hinge V,Patil H,Nadaf A

更新日期：2016-02-01 00:00:00

abstract：:Arabidopsis transcription factor APETALA2 (AP2) controls multiple aspects of plant growth and development, including seed development, stem cell maintenance, and specification of floral organ identity. Based on sequence similar of Arabidopsis AP2 and its homologues genes from other plant species, degenerate RT-PCR and...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Jiang J,Yang Y,Li P,Chen S,Zhang Z

更新日期：2012-09-01 00:00:00

abstract：:The effect of surfactant on enzymatic digestibility was investigated during the pretreatment stage. Newspaper was pretreated with an ammonia-hydrogen peroxide mixture on a shaking bath at 40 degrees C and 130 strokes/min for 3 h. Two kinds of nonionic surfactants, NP series and Tween series, were utilized. The effect ...

journal_title：Applied biochemistry and biotechnology

authors： Kim SB,Chun JW

更新日期：2004-04-01 00:00:00

abstract：:In previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the YR-1 strain of Mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sAO) activity. In the present work enzym...

journal_title：Applied biochemistry and biotechnology

authors： Silva-Jiménez H,Zazueta-Sandoval R

更新日期：2005-04-01 00:00:00

abstract：:In the present investigation, thermophilic fungus Rhizomucor pusillus was used to study biotransformation of antihelmintic drug albendazole to produce its active metabolite, albendazole sulfoxide and novel metabolites of commercial interest. A two-stage fermentation procedure was followed for biotransformation of albe...

journal_title：Applied biochemistry and biotechnology

authors： Prasad GS,Girisham S,Reddy SM

更新日期：2011-11-01 00:00:00

abstract：:Microbial lipid is a promising source of oil to produce biofuel if it can be generated from lignocellulosic materials. Mortierella isabellina is a filamentous fungal species featuring high content of oil in its cell biomass. In this work, M. isabellina was studied for lipid production from corn stover. The experimenta...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Hu B

更新日期：2014-09-01 00:00:00

abstract：:Since the adult mammalian heart has limited regenerative capacity, cardiac trauma, disease, and aging cause permanent loss of contractile tissue. This has fueled the development of stem cell-based strategies to provide the damaged heart with new cardiomyocytes. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are ...

journal_title：Applied biochemistry and biotechnology

authors： Neshati V,Mollazadeh S,Fazly Bazzaz BS,de Vries AAF,Mojarrad M,Naderi-Meshkin H,Neshati Z,Mirahmadi M,Kerachian MA

更新日期：2018-09-01 00:00:00

abstract：:The antioxidant activity of a novel artificial glutathione peroxidase-like enzyme, selenium-containing glutathione 5-transferase from Lucilia cuprina (seleno-LuGST1-1), was studied by using a ferrous sulfate/ascorbate-induced mitochondrial damage model system. Swelling of mitochondria, lipid peroxidation, and cytochro...

journal_title：Applied biochemistry and biotechnology

authors： Yu H,Liu J,Li J,Zang T,Luo G,Shen J

更新日期：2005-11-01 00:00:00

abstract：:The biosurfactant-producing Pseudomonas aeruginosa A11, with plant-growth-promoting (PGP) and multi-metal-resistant (MMR) features was isolated from the rhizosphere of a wild plant Parthenium hysterophorus. The strain A11 was able to utilize glycerol as a carbon source and produce 4,436.9 mg/L of biosurfactant after 1...

journal_title：Applied biochemistry and biotechnology

authors： Singh AK,Cameotra SS

更新日期：2013-07-01 00:00:00

abstract：:Search for efficient low-cost substrate/additives are gaining significant impetus in bacterial cellulose (BC) production. Makgeolli sludge (a traditional Korean wine distillery waste) is enriched with organic acid, alcohol, and sugar. Using makgeolli sludge filtrate (MSF) and Hestrin-Schramm (HS) medium (g/l of distil...

journal_title：Applied biochemistry and biotechnology

authors： Hyun JY,Mahanty B,Kim CG

更新日期：2014-04-01 00:00:00

abstract：:In this article, two theories are unified to investigate the effect of hydrodynamics on a specific bioprocess: the network-of-zones (NOZ) hydrodynamic structured modeling approach (developed by several researchers but applied to only a few bioprocesses) and the effectiveness factor eta approach. Two process scales wer...

journal_title：Applied biochemistry and biotechnology

authors： Delvigne F,El Mejdoub T,Destain J,Delroisse JM,Vandenbol M,Haubruge E,Thonart P

更新日期：2005-04-01 00:00:00

abstract：:Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techni...

journal_title：Applied biochemistry and biotechnology

authors： Ximenes EA,Brandon SK,Doran-Peterson J

更新日期：2008-03-01 00:00:00

abstract：:To investigate the effect of pH and temperature on the cell growth and bacteriocin production of Pediococcus acidilactici PA003, a lactic acid bacterium isolated from traditionally fermented cabbage, the kinetic behaviour of P. acidilactici PA003 was simulated in vitro during laboratory fermentations by making use of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Zhang Y,Liu SN,Han Y,Zhou ZJ

更新日期：2012-03-01 00:00:00

abstract：:Protein-protein interactions (PPIs) play pivotal roles in most of the biological processes. PPI dysfunctions are therefore associated with disease situations. Mutations often lead to PPI dysfunctions, but there are certain other types of mutations which do not cause any appreciable abnormalities. This second type of m...

journal_title：Applied biochemistry and biotechnology

authors： Ali A,Biswas R,Bhattacharjee S,Nath P,Pan S,Bagchi A

更新日期：2016-09-01 00:00:00

abstract：:The amount of corn stover and wheat straw that can be sustainably collected in North Carolina was estimated to be 0.64 and 0.16 million dry t/yr, respectively. More than 80% of these crop residues are located in the coastal area. The bioethanol potential from corn stover and wheat straw was estimated to be about 238 m...

journal_title：Applied biochemistry and biotechnology

authors： Shahbazi A,Li Y

更新日期：2006-04-01 00:00:00

abstract：:Jonesia denitrificans BN-13 produces six xylanases: Xyl1, Xyl2, Xyl3, Xyl4, Xyl5, and Xyl6; the Xyl4 was purified and characterized after two consecutive purification steps using ultrafiltration and anion exchange chromatography. The xylanase-specific activity was found to be 77 unit (U)/mg. The molecular weight of th...

journal_title：Applied biochemistry and biotechnology

authors： Boucherba N,Gagaoua M,Copinet E,Bettache A,Duchiron F,Benallaoua S

更新日期：2014-03-01 00:00:00

abstract：:The objective of this study was to evaluate the capability of a lipopolysaccharide, produced by Trichosporon mycotoxinivorans CLA2 using residue of biodiesel processing, to flocculate two different solids in suspension. In addition, the emulsifying activity and the stability of this lipopolysaccharide in response to p...

journal_title：Applied biochemistry and biotechnology

authors： Domingues VS,Monteiro AS,Ferreira GF,Santos VL

更新日期：2017-05-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:Cationic 1,2-dioleoyl trimethyl ammonium propane (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) are anchored on cysteamine (cyst), mercaptopropionic acid (MPA) monolayer (thiol monolayers) modified on an individual gold transducer. DOTAP and DOPE are mixed with gold nanoparticle (AuNP) to for...

journal_title：Applied biochemistry and biotechnology

authors： Bhuvana M,Dharuman V

更新日期：2014-10-01 00:00:00

abstract：:A strain of Clostridium thermoaceticum (ATCC 49707) was evaluated for its homoacetate potential. This thermophilic anaerobe best produces acetate from glucose at pH 6.0 and 59 degrees C with a yield of 83% of theoretical. Enzyme hydrolysis of two substrates, a-cellulose and a pulp mill sludge, yielded 68% and 70% dige...

journal_title：Applied biochemistry and biotechnology

authors： Borden JR,Lee YY,Yoon HH

更新日期：2000-04-01 00:00:00