Immobilization of Escherichia coli novablue gamma-glutamyltranspeptidase in Ca-alginate-kappa-carrageenan beads.

Abstract:

:The recombinant Escherichia coli gamma-glutamyltranspeptidase (EcGGT) was immobilized in Ca-alginate-kappa-carrageenan beads. Effects of alginate concentration, amount of loading enzyme, and bead size on the entrapped activity were investigated. Optimum alginate concentration for EcGGT immobilization was found to be 2% (w/v). Using a loading enzyme concentration of 1.5 mg/g alginate, maximum enzyme activity was observed. With increase in bead size from 1.9 to 3.1 mm, the immobilization efficiency was decreased significantly because of mass transfer resistance. Thermal stability of the free EcGGT was increased as a result of the immobilization. Ca-alginate-kappa-carrageenan-EcGGT beads were suitable for up to six repeated uses, losing only 45% of their initial activity. Upon 30 days of storage the preserved activity of free and immobilized enzyme were found as 4% and 68%, respectively. The synthesis of L: -theanine was performed in 50 mM Tris-HCl buffer (pH 10) containing 25 mM L: -glutamine, 40 mM ethylamine, and 1.5 mg EcGGT/g alginate at 40 degrees C for 12 h, and a conversion rate of 27% was achieved.

authors

Hung CP,Lo HF,Hsu WH,Chen SC,Lin LL

doi

10.1007/s12010-008-8244-x

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

157-70

issue

2

eissn

0273-2289

issn

1559-0291

journal_volume

150

pub_type

杂志文章
  • Biotechnological and molecular approaches for vanillin production: a review.

    abstract::Vanillin is one of the most widely used flavoring agents in the world. As the annual world market demand of vanillin could not be met by natural extraction, chemical synthesis, or tissue culture technology, thus biotechnological approaches may be replacement routes to make production of bio-vanillin economically viabl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s12010-012-0066-1

    authors: Kaur B,Chakraborty D

    更新日期:2013-02-01 00:00:00

  • High-yield hypocrellin A production in solid-state fermentation by Shiraia sp. SUPER-H168.

    abstract::Hypocrellin A production by Shiraia sp. SUPER-H168 was studied under solid-state fermentation. Corn was found to be the best substrate after evaluating eight kinds of agro-industrial crops and residues. The optimized solid-state fermentation conditions were as follows: inoculum size 3 x 10(6) spores, substrate particl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8728-3

    authors: Cai Y,Liang X,Liao X,Ding Y,Sun J,Li X

    更新日期:2010-04-01 00:00:00

  • Influence of forced internal air circulation on airflow distribution and heat transfer in a gas double-dynamic solid-state fermentation bioreactor.

    abstract::Internal air circulation affects the temperature field distribution in a gas double-dynamic solid-state fermentation bioreactor (GDSFB). To enhance heat transfer through strengthening internal air circulation in a GDSFB, we put an air distribution plate (ADP) into the bioreactor and studied the effects of forced inter...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0670-8

    authors: Chen H,Qin L,Li H

    更新日期:2014-02-01 00:00:00

  • Phytosterols elevation in bamboo shoot residue through laboratorial scale solid-state fermentation using isolated Aspergillus niger CTBU.

    abstract::Aspergillus niger CTBU isolated from local decayed bamboo shoot residue was employed to solid-state fermentation (SSF) of bamboo shoot residue to elevate the content of phytosterols. Strain acclimatization was carried out under the fermentation condition using bamboo shoot as substrate for fermentation performance imp...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0821-6

    authors: Zheng XX,Chen RS,Shen Y,Yin ZY

    更新日期:2014-04-01 00:00:00

  • Immobilization of L-Asparaginase on Magnetic Nanoparticles for Cancer Treatment.

    abstract::In this presented work, magnetic poly(HEMA-GMA) nanoparticles were synthesized, characterized, and used for immobilization of an anti-leukemic enzyme L-asparaginase. The average particle size of the synthesized magnetic nanoparticles was found to be as 117.5 nm. L-asparaginase was successfully immobilized onto the syn...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03276-z

    authors: Orhan H,Aktaş Uygun D

    更新日期:2020-08-01 00:00:00

  • Feasibility of treating swine manure in an anaerobic sequencing batch biofilm reactor with mechanical stirring.

    abstract::Anaerobic sequencing batch reactors containing granular or flocculent biomass have been employed successfully in the treatment of piggery wastewater. However, the studies in which these reactors were employed did not focus specifically on accelerating the hydrolysis step, even though the degradation of this chemical o...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:120:2:109

    authors: de Pinho SC,Fernandes BS,Rodrigues JA,Ratusznei SM,Foresti E,Zaiat M

    更新日期:2005-02-01 00:00:00

  • Efficient Antibody Assembly in E. coli Periplasm by Disulfide Bond Folding Factor Co-expression and Culture Optimization.

    abstract::Molecular chaperones and protein folding factors of bacterial periplasmic space play important roles in assisting disulfide bond formation and proper protein folding. In this study, effects of disulfide bond protein (Dsb) families were investigated on assembly of 3F3 Fab, an antibody inhibitor targeting matrix metallo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2502-8

    authors: Rodriguez C,Nam DH,Kruchowy E,Ge X

    更新日期:2017-10-01 00:00:00

  • Improvement in thermal stability and substrate binding of pig kidney D-amino acid oxidase by chemical modification.

    abstract::Chemical modification was evaluated to stabilize pig kidney D-amino acid oxidase (pkDAAO), which is required for analytical determination of D-amino acids. Optimization of modification conditions was performed to obtain high recovery yield and stability, and chemical modification at 30 degrees C for 12 h with a highly...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:112:3:123

    authors: Bakke M,Kajiyama N

    更新日期:2004-03-01 00:00:00

  • Preserving the activity of cellulase in a batch foam fractionation process.

    abstract::Foam fractionation is one of the low operating-cost techniques for removing proteins from a dilute solution. The initial bulk solution pH and air superficial velocity play an important role in the foam-fractionation process. Denaturation of proteins (enzymes) can occur, however, during the foam-fractionation process f...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:79:1-3:701

    authors: Loha V,Prokop A,Du L,Tanner RD

    更新日期:1999-04-01 00:00:00

  • Isolation and functional characterization of a novel seed-specific promoter region from peanut.

    abstract::The importance of using tissue-specific promoters in the genetic transformation of plants has been emphasized increasingly. Here, we report the isolation of a novel seed-specific promoter region from peanut and its validation in Arabidopsis and tobacco seeds. The reported promoter region referred to as groundnut seed ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0482-x

    authors: Sunkara S,Bhatnagar-Mathur P,Sharma KK

    更新日期:2014-01-01 00:00:00

  • Enhancement of long-chain fatty acid production in Escherichia coli by coexpressing genes, including fabF, involved in the elongation cycle of fatty acid biosynthesis.

    abstract::The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9987-y

    authors: Lee S,Lee S,Yoon YJ,Lee J

    更新日期:2013-01-01 00:00:00

  • Real-Time and Online Monitoring of Glucose Contents by Using Molecular Imprinted Polymer-Based IDEs Sensor.

    abstract::A highly sensitive, selective, reversible, and reusable glucose sensor is developed by using molecularly imprinted polymer-based artificial receptors onto interdigital transducer. Sensor receptors were synthesized through bulk imprinting technology by using styrene as monomer, ethylene glycol dimethacrylate (EGDMA) as...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03049-3

    authors: Asghar N,Mustafa G,Yasinzai M,Al-Soud YA,Lieberzeit PA,Latif U

    更新日期:2019-12-01 00:00:00

  • Prediction of folding stability and degradability of the de novo designed protein MB-1 in cow rumen.

    abstract::The authors have recently reported on the design of a protein (MB-1) enriched in methionine, threonine, lysine, and leucine. The protein is intended to be produced by rumen bacteria, in a way that would provide high producing lactating cows with limiting amino acids. In this report, MB-1 stability in the rumen is asse...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02788809

    authors: MacCallum JD,Hefford MA,Omar S,Beauregard M

    更新日期:1997-04-01 00:00:00

  • Regeneration of nicotinamide cofactors for use in organic synthesis.

    abstract::The high cost of nicotinamide cofactors requires that they be regenerated in situ when used in preparative enzymatic synthesis. Numerous strategies have been tested for in situ regeneration of reduced and oxidized cofactors. Regeneration of reduced cofactors is relatively straightforward; regeneration of oxidized cofa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/BF02798431

    authors: Chenault HK,Whitesides GM

    更新日期:1987-03-01 00:00:00

  • Understanding pH and ionic strength effects on aluminum sulfate-induced microalgae flocculation.

    abstract::The objective of this study was to understand the effect of pH and ionic strength of aluminum sulfate on the flocculation of microalgae. It was found that changing pH and ionic strength influenced algal flocculation by changing the zeta potential of cells, which was described by the classical theory of Derjaguin, Land...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0957-4

    authors: Cui Y,Yuan W,Cheng J

    更新日期:2014-08-01 00:00:00

  • Modeling the Effect of pH and Temperature for Cellulases Immobilized on Enzymogel Nanoparticles.

    abstract::Production costs of cellulosic biofuels can be lowered if cellulases are recovered and reused using particulate carriers that can be extracted after biomass hydrolysis. Such enzyme recovery was recently demonstrated using enzymogel nanoparticles with grafted polymer brushes loaded with cellulases. In this work, cellul...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1633-z

    authors: Samaratunga A,Kudina O,Nahar N,Zakharchenko A,Minko S,Voronov A,Pryor SW

    更新日期:2015-06-01 00:00:00

  • Production of biodiesel by immobilized Candida sp. lipase at high water content.

    abstract::A new process for enzymatic synthesis of biodiesel at high water content (10-20%) with 96% conversion by lipase from Candida sp. 99-125 was studied. The lipase, a no-position-specific lipase, was immobilized by a cheap cotton membrane and the membrane-immobilized lipase could be used at least six times with high conve...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:128:2:109

    authors: Tan T,Nie K,Wang F

    更新日期:2006-02-01 00:00:00

  • L-Asparaginase as potent anti-leukemic agent and its significance of having reduced glutaminase side activity for better treatment of acute lymphoblastic leukaemia.

    abstract::Acute lymphoblastic leukaemia (ALL) is one of the leading types of malignant disorder seen in children. Viral infections, genetic factors and exposure to chemical carcinogens are some of the factors responsible for causing ALL. Treatment strategies followed for curing ALL include chemotherapy or radiation therapy, whe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s12010-012-9755-z

    authors: Ramya LN,Doble M,Rekha VP,Pulicherla KK

    更新日期:2012-08-01 00:00:00

  • Continuous production of butanol from starch-based packing peanuts.

    abstract::Acetone, butanol, ethanol (ABE, or solvents) were produced from starch-based packing peanuts in batch and continuous reactors. In a batch reactor, 18.9 g/L of total ABE was produced from 80 g/L packing peanuts in 110 h of fermentation. The initial and final starch concentrations were 69.6 and 11.1 g/L, respectively. I...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:106:1-3:375

    authors: Ezeji TC,Groberg M,Qureshi N,Blaschek HP

    更新日期:2003-04-01 00:00:00

  • Conversion of isoeugenol to vanillin by Psychrobacter sp. strain CSW4.

    abstract::To screen strains of halotolerant or halophile bacteria which are able to convert isoeugenol to vanillin, 36 different strains of bacteria isolated from the salty environments in Iran were investigated. During growth on isoeugenol, a moderately halotolerant Gram-negative coccobacil showed capability of converting isoe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9397-6

    authors: Ashengroph M,Nahvi I,Zarkesh-Esfahani H,Momenbeik F

    更新日期:2012-01-01 00:00:00

  • Reusability of entrapped cells of Pseudomonas diminuta for production of 7-aminocephalosporanic acid.

    abstract::Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA with entrapped cells of P. diminuta in ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-9214-4

    authors: Nigam VK,Kundu S,Ghosh P

    更新日期:2007-04-01 00:00:00

  • Drug Design for ALK-Positive NSCLC: an Integrated Pharmacophore-Based 3D QSAR and Virtual Screening Strategy.

    abstract::The increasing death rates related to anaplastic lymphoma kinase (ALK)-positive lung cancer culminated in a significant interest in the discovery of novel inhibitors for ALK. In the present research work, pharmacophore-based 3D QSAR modeling and virtual screening strategy have been carried out to address these issues....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2650-x

    authors: James N,Shanthi V,Ramanathan K

    更新日期:2018-05-01 00:00:00

  • Comparison of One-Stage Batch and Fed-Batch Enzymatic Hydrolysis of Pretreated Hardwood for the Production of Biosugar.

    abstract::Fed-batch method has shown a great promise in debottlenecking the high-solid enzymatic hydrolysis for the commercialization of cellulosic biosugar conversion for biofuel/biochemical production. To further improve enzymatic hydrolysis efficiency at high solid loading, fed-batch methods of green liquor-pretreated hardwo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2633-y

    authors: He L,Han Q,Jameel H,Chang HM,Phillips R,Wang Z

    更新日期:2018-04-01 00:00:00

  • Transition metals and organic ligands influence biodegradation of 1,4-dioxane.

    abstract::1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0841-2

    authors: Pornwongthong P,Mulchandani A,Gedalanga PB,Mahendra S

    更新日期:2014-05-01 00:00:00

  • Isolation and characterization of a novel feather-degrading bacterial strain.

    abstract::Feather waste, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Feather-degrading bacteria were isolated from a Brazilian poultry industrial waste. Among these isolates, a strain identified as kr2 was th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:87:1:17

    authors: Sangali S,Brandelli A

    更新日期:2000-04-01 00:00:00

  • Mechanism of DNA-drug interactions.

    abstract::Over the last two decades many strategies have been planned to design specific drugs for rare diseases to target their action at the DNA level. Advancements in our understanding of the interactions of small nonpeptide molecules with DNA have opened the doors for "rational" drug design. Special methods have now been de...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/BF02788674

    authors: Prabhakar P,Kayastha AM

    更新日期:1994-04-01 00:00:00

  • Efficiency and stability enhancement of cis-epoxysuccinic acid hydrolase by fusion with a carbohydrate binding module and immobilization onto cellulose.

    abstract::Cis-epoxysuccinic acid hydrolase (CESH) is an enzyme that catalyzes cis-epoxysuccinic acid to produce enantiomeric L(+)-tartaric acid. The production of tartaric acid by using CESH would be valuable in the chemical industry because of its high yield and selectivity, but the low stability of CESH hampers its applicatio...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9811-8

    authors: Wang S,Cui GZ,Song XF,Feng Y,Cui Q

    更新日期:2012-10-01 00:00:00

  • Development of a method to quantify gene expression levels for glycosylation pathway genes in chinese hamster ovary cell cultures.

    abstract::Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:125:3:159

    authors: Clark KJ,Harcum SW,Griffiths J,Bailey KM

    更新日期:2005-06-01 00:00:00

  • Study on photoconductivity of dye-polymer-based solid-state thin film.

    abstract::In this article, we describe a solid-state photoelectrochemical cell for light detection. Safranine-T dye mixed with polyvinyl alcohol (PVA) solution was deposited on a conducting and transparent indium-tin-oxide (ITO)-coated glass that was used as one electrode and another ITO-coated glass as the counterelectrode. A ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:96:1-3:055

    authors: Dey SK,Manik NB

    更新日期:2001-10-01 00:00:00

  • Chitosan-modified carbon nanotubes-based platform for low-density lipoprotein detection.

    abstract::We have fabricated an immunosensor based on carbon nanotubes and chitosan (CNT-CH) composite for detection of low density lipoprotein (LDL) molecules via electrochemical impedance technique. The CNT-CH composite deposited on indium tin oxide (ITO)-coated glass electrode has been used to covalently interact with anti-a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1179-5

    authors: Ali MA,Singh N,Srivastava S,Agrawal VV,John R,Onoda M,Malhotra BD

    更新日期:2014-10-01 00:00:00