Chitosan-modified carbon nanotubes-based platform for low-density lipoprotein detection.

abstract：:Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 106 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structu...

journal_title：Applied biochemistry and biotechnology

authors： Xu P,Yuan R,Hou G,Li J,Ye M

更新日期：2018-06-01 00:00:00

abstract：:L-asparaginase production was optimized using isolated Bacillus circulans (MTCC 8574) under solid-state fermentation (SSF) using locally available agricultural waste materials. Among different agricultural materials (red gram husk, bengal gram husk, coconut, and groundnut cake), red gram husk gave the maximum enzyme p...

journal_title：Applied biochemistry and biotechnology

authors： Hymavathi M,Sathish T,Subba Rao Ch,Prakasham RS

更新日期：2009-10-01 00:00:00

abstract：:In plants, the final step of cysteine formation is catalyzed by O-acetylserine (thiol) lyase (OAS-TL). The purpose of this study was to isolate and characterize an OAS-TL from the tree legume Leucaena leucocephala (leucaena). Leucaena contains a toxic, nonprotein amino acid, mimosine, which is also formed by an OAS-TL...

journal_title：Applied biochemistry and biotechnology

authors： Yafuso JT,Negi VS,Bingham JP,Borthakur D

更新日期：2014-07-01 00:00:00

abstract：:Flavonoids are secondary metabolites synthesized by plants shown to exhibit health benefits such as anti-inflammatory, antioxidant, and anti-tumor effects. Thus, due to the importance of this compound, several enzymes involved in the flavonoid pathway have been cloned and characterized in Escherichia coli. However, th...

journal_title：Applied biochemistry and biotechnology

authors： Zakaria II,Rahman RN,Salleh AB,Basri M

更新日期：2011-09-01 00:00:00

abstract：:L-Asparaginase (ASNase) has proved its use in medical and food industries. Sequence-based screening showed the thermophilic Streptomyces strain Streptomyces thermoluteus subsp. fuscus NBRC 14270 (14270 ASNase) to positive against predicted ASNase primary sequences. The 14270 ASNase gene and four L-asparaginase genes f...

journal_title：Applied biochemistry and biotechnology

authors： Hatanaka T,Usuki H,Arima J,Uesugi Y,Yamamoto Y,Kumagai Y,Yamasato A,Mukaihara T

更新日期：2011-04-01 00:00:00

abstract：:This study presents the production of biodiesel from algae oil by transesterification using thermophilic microorganism. The microorganism used in this study was isolated from the soil sample obtained near the furnace. The organism was identified as Bacillus sp., and the lipase obtained was purified by ammonium sulfate...

journal_title：Applied biochemistry and biotechnology

authors： Sivaramakrishnan R,Muthukumar K

更新日期：2012-02-01 00:00:00

abstract：:The glpX gene from Francisella tularensis encodes for the class II fructose 1,6-bisphosphatase (FBPaseII) enzyme. The glpX gene has been verified to be essential in F. tularensis, and the inactivation of this gene leads to impaired bacterial growth on gluconeogenic substrates. In the present work, we have complemented...

journal_title：Applied biochemistry and biotechnology

authors： Gutka HJ,Wolf NM,Bondoc JMG,Movahedzadeh F

更新日期：2017-12-01 00:00:00

abstract：:In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus repo...

journal_title：Applied biochemistry and biotechnology

authors： Liu Y,Yang SX,Cheng Y,Liu DQ,Zhang Y,Deng KJ,Zheng XL

更新日期：2015-12-01 00:00:00

abstract：:The oil palm sector is one of the major plantation industries in Malaysia. Palm kernel cake is a byproduct of extracted palm kernel oil. Mostly palm kernel cake is wasted or is mixed with other nutrients and used as animal feed, especially for ruminant animals. Recently, palm kernel cake has been identified as an impo...

journal_title：Applied biochemistry and biotechnology

authors： Ong LG,Abd-Aziz S,Noraini S,Karim MI,Hassan MA

更新日期：2004-07-01 00:00:00

abstract：:Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009. Using new expression tools developed at...

journal_title：Applied biochemistry and biotechnology

authors： Brumm P,Mead D,Boyum J,Drinkwater C,Deneke J,Gowda K,Stevenson D,Weimer P

更新日期：2011-03-01 00:00:00

abstract：:S-Adenosylhomocysteine hydrolase (SAHase) encoded by sahase gene is a determinant when catalyzing the reversible conversion of adenosine and homocysteine to S-adenosylhomocysteine in most living organisms. The sahase gene was isolated from the genome of the highly thermostable anaerobic bacteria Thermotoga maritima, a...

journal_title：Applied biochemistry and biotechnology

authors： Lozada-Ramírez JD,Sánchez-Ferrer A,García-Carmona F

更新日期：2013-06-01 00:00:00

abstract：:The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The rea...

journal_title：Applied biochemistry and biotechnology

authors： Marsaoui N,Naghmouchi K,Baah J,Raies A,Laplante S

更新日期：2015-06-01 00:00:00

abstract：:To determine the influence of various culture conditions, transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were grown in nine cultures with four variable conditions (storage of inoculated broth at 4 degrees C prior to incubation, agitation speed, isopropyl-beta-D-thiogalac...

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Ishii M,Junior AP,de Oliveira Nascimento L,de Souza LC,Cholewa O

更新日期：2004-04-01 00:00:00

abstract：:Numerous microcapsule systems have been developed for a wide range of applications, including the sustained release of drugs, cell transplantation for therapy, cell immobilization, and other biotechnological applications. Despite the fact that microcapsule membrane is a dominant factor governing overall microcapsule p...

journal_title：Applied biochemistry and biotechnology

authors： Chen H,Ouyang W,Lawuyi B,Lim T,Prakash S

更新日期：2006-09-01 00:00:00

abstract：:A review of studies of biomass potential in the United States finds a wide variation in the estimates. A number of specific policy-relevant questions about the potential of biofuels in the United States are answered. A recently published global analysis of the potential conflict between land needed for bioenergy and l...

journal_title：Applied biochemistry and biotechnology

authors： Converse AO

更新日期：2007-04-01 00:00:00

abstract：:Solid-state fermentation, featured by water-saving, eco-friendly and high concentration product, is a promising technology in lignocellulosic ethanol industry. However, in solid-state fermentation system, large gas content inside the substrate directly leads to high oxygen partial pressure and inhibits ethanol ferment...

journal_title：Applied biochemistry and biotechnology

authors： Sun L,Wang L,Chen H

更新日期：2020-10-01 00:00:00

abstract：:The enzyme 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGR) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. This enzyme has three domains: transmembrane, binding, and soluble. In this study, we expressed and puri...

journal_title：Applied biochemistry and biotechnology

authors： Andrade-Pavón D,Cuevas-Hernández RI,Trujillo-Ferrara JG,Hernández-Rodríguez C,Ibarra JA,Villa-Tanaca L

更新日期：2017-08-01 00:00:00

abstract：:Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in...

journal_title：Applied biochemistry and biotechnology

authors： Mohan A,Nair SV,Lakshmanan VK

更新日期：2017-04-01 00:00:00

abstract：:Endophytes from medicinal plants represent a potential source of bioactive compounds. During the present investigation, fungal endophytes were isolated from turmeric (Curcuma longa), an important medicinal plant. A total of 207 endophytic fungal isolates were obtained from the rhizome of C. longa L. They were grouped ...

journal_title：Applied biochemistry and biotechnology

authors： Gupta S,Kaul S,Singh B,Vishwakarma RA,Dhar MK

更新日期：2016-11-01 00:00:00

abstract：:Phenoloxidase (PO) is a key enzyme in insect development, responsible for catalyzing the hydroxylation of tyrosine into o-diphenols and the oxidation of o-diphenols into o-quinones. In the present study, the kinetic assay in air-saturated solutions and the kinetic behavior of PO from Pieris rapae (Lepidoptera) larvae ...

journal_title：Applied biochemistry and biotechnology

authors： Xue CB,Luo WC,Jiang L,Xie XY,Xiao T,Yan L

更新日期：2007-11-01 00:00:00

abstract：:Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from ei...

journal_title：Applied biochemistry and biotechnology

authors： Thierfelder S,Ostermann K,Göbel A,Rödel G

更新日期：2011-04-01 00:00:00

abstract：:The microheterogeneous native amylolytic complex secreted by the isolate A6 of Lactobacillus plantarum revealed a selective enzyme specificity loss when submitted to a limited proteolysis under a suboptimum pH condition. A clear electrophoretic profile change toward just one shorter, more acidic, and equally active po...

journal_title：Applied biochemistry and biotechnology

authors： Florêncio JA,Eiras-Stofella DR,Soccol CR,Raimbault M,Guyot JP,Fontana JD

更新日期：2000-04-01 00:00:00

abstract：:Vanillin is one of the most widely used flavoring agents in the world. As the annual world market demand of vanillin could not be met by natural extraction, chemical synthesis, or tissue culture technology, thus biotechnological approaches may be replacement routes to make production of bio-vanillin economically viabl...

journal_title：Applied biochemistry and biotechnology

authors： Kaur B,Chakraborty D

更新日期：2013-02-01 00:00:00

abstract：:A new method without any special devices for direct transformation of linear gene cassettes was developed. Its feasibility was verified through 5'-fluorescent dye (fluorescein isothiocyanate, FITC)-labeled fluorescent tracing and transient expression of a gus reporter gene. Minimal linear gene cassettes, containing ne...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YQ,Yang J,Xu FP,An LJ,Liu JF,Chen ZW

更新日期：2009-12-01 00:00:00

abstract：:In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high eth...

journal_title：Applied biochemistry and biotechnology

authors： Ding J,Gao M,Hou G,Liang K

更新日期：2015-08-01 00:00:00

abstract：:A putative α-amylase gene (accession number, CP000284) of Methylobacillus flagellatus KT ATCC51484 was cloned in Escherichia coli, and its gene product was expressed and characterized. The purified recombinant enzyme (MFAS) displayed a typical amylosucrase (ASase) activity by the demonstration of multiple activities o...

journal_title：Applied biochemistry and biotechnology

authors： Jeong JW,Seo DH,Jung JH,Park JH,Baek NI,Kim MJ,Park CS

更新日期：2014-06-01 00:00:00

abstract：:Two DNA fragments containing the entire coding sequences of lactate dehydrogenase (LDH; ldhL1 and ldhD), whose enzymes have high activity for bioconversion of phenylpyruvate (PPA) to phenyllactate (PLA), were amplified from Lactobacillus plantarum SK002 using PCR. Sequencing showed open reading frames of 963 bp (ldhL1...

journal_title：Applied biochemistry and biotechnology

authors： Jia J,Mu W,Zhang T,Jiang B

更新日期：2010-09-01 00:00:00

abstract：:This study evaluates the potential for using different effluents for simultaneous H(2) and CH(4) production in a two-stage batch fermentation process with mixed microflora. An appreciable amount of H(2) was produced from parboiled rice wastewater (23.9 mL g(-1) chemical oxygen demand [COD]) and vinasse (20.8 mL g(-1) ...

journal_title：Applied biochemistry and biotechnology

authors： Peixoto G,Pantoja-Filho JL,Agnelli JA,Barboza M,Zaiat M

更新日期：2012-10-01 00:00:00

abstract：:Phytochelatin synthase (PCS) is a key enzyme involved in the synthesis of phytochelatins, which are thought to play important roles in heavy metal detoxification. The sacred lotus (Nelumbo nucifera), one of the most popular ornamental species, has been shown to be a potential phytoremediator of heavy metal polluted wa...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Yang D,Wu K,Chen S,Jiang J,Zhang Z

更新日期：2012-02-01 00:00:00

abstract：:The genome sequence, morphology, and genetic features of a novel phage, named SSE1, is reported here. Phage SSE1 that infects Shigella dysenteriae (China General Microbiological Culture Collection Center number: 1.1869) was isolated from the aeration tank water of a sewage treatment plant. SSE1 showed morphological fe...

journal_title：Applied biochemistry and biotechnology

authors： Lu H,Liu H,Lu M,Wang J,Liu X,Liu R

更新日期：2020-09-01 00:00:00