Abstract：
:A new method without any special devices for direct transformation of linear gene cassettes was developed. Its feasibility was verified through 5'-fluorescent dye (fluorescein isothiocyanate, FITC)-labeled fluorescent tracing and transient expression of a gus reporter gene. Minimal linear gene cassettes, containing necessary regulation elements and a gus reporter gene, was prepared by polymerase chain reaction and dissolved in transformation buffer solution to 100 ng/mL. The basic transformation solution used was Murashige and Skoog basal salt mixture (MS) liquid medium. Hypertonic pretreatment of explants and transformation cofactors, including Ca(2+), surfactant assistants, Agrobacterium LBA4404 cell culture on transformation efficiency were evaluated. Prior to the incubation of the explants and target linear cassette in each designed transformation solution for 3 h, the onion low epidermal explants were pre-cultured in darkness at 27 degrees C for 48 h and then transferred to MS solid media for 72 h. FITC-labeled linear DNA was used to trace the delivery of DNA entry into the cell and the nuclei. By GUS staining and flow-cytometry-mediated fluorescent detection, a significant increase of the ratios of fluorescent nuclei as well as expression of the gus reporter gene was observed by each designed transformation solution. This potent and feasible method showed prospective applications in plant transgenic research.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Cheng YQ,Yang J,Xu FP,An LJ,Liu JF,Chen ZWdoi
10.1007/s12010-009-8554-7subject
Has Abstractpub_date
2009-12-01 00:00:00pages
739-49issue
3eissn
0273-2289issn
1559-0291journal_volume
159pub_type
杂志文章abstract：:The aim of the present study was to investigate pectinases production by CT1 mutant of Penicillium occitanis on glucose based media. Two main groups of pectinases were followed: lyases (pectin and pectate lyases) and hydrolases (polygalacturonases and polymethylgalacturonases). When cultivated in different liquid medi...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-012-9971-6
更新日期：2013-01-01 00:00:00
abstract：:Biomass degradation compounds significantly inhibit biochemical conversion of biomass prehydrolysates to biofuels and chemicals, such as lactic acid. To characterize the structure-activity relationship of carbonyl inhibition on lactic acid fermentation, we examined effects of eight carbonyl compounds (furfural, 5-hydr...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1533-2
更新日期：2015-04-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
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doi：10.1007/s12010-014-1337-9
更新日期：2015-01-01 00:00:00
abstract：:Empty palm fruit bunch fiber (EPFBF), a readily available cellulosic biomass from palm processing facilities, is investigated as a potential carbohydrate source for cellulosic ethanol production. This feedstock was pretreated using ammonia fiber expansion (AFEX) and enzymatically hydrolyzed. The best tested AFEX condi...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-010-8962-8
更新日期：2010-11-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-018-02934-7
更新日期：2019-06-01 00:00:00
abstract：:1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-0841-2
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journal_title：Applied biochemistry and biotechnology
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doi：10.1007/s12010-016-2202-9
更新日期：2017-01-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-009-8827-1
更新日期：2010-05-01 00:00:00
abstract：:The emergence of nalidixic acid-resistant strains of Salmonella typhimurium remains to be a major public health problem. In particular, the substitution of Asn in place of Asp at the 87 loci in the GyrA of S. typhimurium was experimentally stated for nalidixic acid resistance. However, the data on the possible mechani...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1760-6
更新日期：2015-09-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2122-8
更新日期：2016-10-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
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doi：10.1007/s12010-009-8531-1
更新日期：2009-04-01 00:00:00
abstract：:The effect of organic matter and fill time on anaerobic sequencing batch reactor (5 L, 30 °C, 8-h cycles, 50 rpm) efficiency has been analyzed. Organic matter was increased by the influent concentration. Fill times investigated were in the batch mode and fed-batch followed by batch. In the batch mode organic matter re...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-010-9009-x
更新日期：2010-12-01 00:00:00
abstract：:Lignocellulose biomass contain large macromolecules especially cellulose and hemicelluloses that can be converted to fuel and chemicals using microbial biocatalysts. This study presents comprehensive optimization of production of biomass-hydrolyzing enzymes (BHE) by a high β-glucosidase-producing Trichoderma SG2 for b...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-020-03258-1
更新日期：2020-05-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02788674
更新日期：1994-04-01 00:00:00
abstract：:The use of viruses for treatment of cancer overcomes the bottlenecks of chemotherapy and radiotherapy. Several viruses and their proteins have been evaluated for oncolytic effect. The VP3 protein (apoptin) of chicken anemia virus is one such protein with an inherent ability to lyse cancer and transformed cells while l...
journal_title：Applied biochemistry and biotechnology
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doi：10.1007/s12010-015-1567-5
更新日期：2015-05-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02798635
更新日期：1982-01-01 00:00:00
abstract：:For producing a large quantity of human nerve growth factor beta (hNGF-beta), the recombinant adenovirus containing an expression cassette of hNGF-beta complementary DNA gene was constructed and then instilled directly into goats' mammary glands. The recombinant hNGF-beta was detected in the milk. The maximal expressi...
journal_title：Applied biochemistry and biotechnology
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doi：10.1007/s12010-008-8346-5
更新日期：2009-06-01 00:00:00
abstract：:Titanium-dioxide nanoparticles (TNP) are used in various consumable goods. Evidence has demonstrated the cytotoxicity of TNPs, but exact mechanism is yet to be elucidated. The present study has been aimed at finding out the mechanism of TNP-induced toxicity in biological system. Different doses of anatase-TNPs adminis...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2165-x
更新日期：2016-12-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
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更新日期：2011-07-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2198-1
更新日期：2017-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-019-03049-3
更新日期：2019-12-01 00:00:00
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更新日期：2018-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-008-8244-x
更新日期：2008-08-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-011-9292-1
更新日期：2011-09-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-1032-x
更新日期：2014-08-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:98-100:1-9:229
更新日期：2002-04-01 00:00:00
abstract：:Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mut...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-0818-1
更新日期：2014-04-01 00:00:00
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journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1588-0
更新日期：2015-05-01 00:00:00
