Heterologous Expression of Aldehyde Dehydrogenase in Lactococcus lactis for Acetaldehyde Detoxification at Low pH.

abstract：:Tobacco plants can be used for the production of proteins for pharmaceutical applications. One of the most difficult and expensive tasks associated with this technology is isolating the product of interest from the hundreds of other chemicals found in tobacco. We describe a new recovery strategy in which the protein o...

journal_title：Applied biochemistry and biotechnology

authors： Crofcheck C,Maiti I,Pattanaik S,Jay M

更新日期：2004-10-01 00:00:00

abstract：:The common view of amyloids and prion proteins is that they are associated with many currently incurable diseases and present a great danger to an organism. This danger comes from the fact that not only prion proteins, but also the infectious form(s) of amyloids, as it has been shown recently, are able to transmit the...

journal_title：Applied biochemistry and biotechnology

authors： Pulawski W,Ghoshdastider U,Andrisano V,Filipek S

更新日期：2012-04-01 00:00:00

abstract：:Oseltamivir (Tamiflu) is the preferred anti-viral drug employed to fight the flu virus in infected individuals. The principal target for this drug is a virus surface glycoprotein, neuraminidase (NA), which facilitates the release of nascent virus and thus spreads infections. Until recently, only a low prevalence of ne...

journal_title：Applied biochemistry and biotechnology

authors： Karthick V,Shanthi V,Rajasekaran R,Ramanathan K

更新日期：2012-05-01 00:00:00

abstract：:Soybean hulls were subjected to thermo-mechanical extrusion pretreatment at various in-barrel moisture contents and screw speeds. Extrusion degraded the lignocellulosic structure and enhanced enzymatic hydrolysis of soybean hulls, with up to 155% increase in glucose yield as compared to untreated substrate. Greater gl...

journal_title：Applied biochemistry and biotechnology

authors： Yoo J,Alavi S,Vadlani P,Behnke KC

更新日期：2012-02-01 00:00:00

abstract：:Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research. Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transfo...

journal_title：Applied biochemistry and biotechnology

authors： Dang X,Shan Z,Chen H

更新日期：2016-11-01 00:00:00

abstract：:Bovine lactoferrampin (LFA) and bovine lactoferricin (LFC) are two antimicrobial peptides located in the N(1) domain of bovine lactoferrin. The bactericidal activity of the fused peptide LFA-LFC is stronger than that of either LFA or LFC. The high cost of peptide production from either native digestion or chemical syn...

journal_title：Applied biochemistry and biotechnology

authors： Tang XS,Tang ZR,Wang SP,Feng ZM,Zhou D,Li TJ,Yin YL

更新日期：2012-02-01 00:00:00

abstract：:Hydrolysates were obtained from dilute sulfuric acid pretreatment of whole-tree softwood forest thinnings and softwood sawdust. Mid-infrared (IR) spectra were obtained on sample sets of wet washed hydrolysates, and 45 degrees C vacuum-dried washed hydrolysates, using a Fourier transform infrared (FTIR) spectrophotomet...

journal_title：Applied biochemistry and biotechnology

authors： Tucker MP,Nguyen QA,Eddy FP,Kadam KL,Gedvilas LM,Webb JD

更新日期：2001-04-01 00:00:00

abstract：:Events from the past growth conditions influence the course of physiology, and it gets reflected in the present cell behaviour. During this process, cells acquire a metabolic option which carries signature of the past, and it dictates the performance in the present situation. Study uses Escherichia coli as a sample or...

journal_title：Applied biochemistry and biotechnology

authors： Puranik S,Purohit HJ

更新日期：2014-11-01 00:00:00

abstract：:Plants generally produce secondary metabolites in nature as a defense mechanism against pathogenic and insect attack. In this study, we applied several abiotic elicitors in order to enhance growth and ginseng saponin biosynthesis in the hairy roots of Panax ginseng. Generally, elicitor treatments were found to inhibit...

journal_title：Applied biochemistry and biotechnology

authors： Jeong GT,Park DH

更新日期：2006-04-01 00:00:00

abstract：:Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant...

journal_title：Applied biochemistry and biotechnology

authors： Gurjar K,Rampuria S,Joshi U,Palit P,Bhatt KV,Mohapatra T,Sharma R

更新日期：2014-08-01 00:00:00

abstract：:A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a disposable hypodermic needle made of stainless steel as the counter electrode. A Nafion membrane, an immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Chen CY,Tamiya E,Ishihara K,Kosugi Y,Su YC,Nakabayashi N,Karube I

更新日期：1992-09-01 00:00:00

abstract：:During glycerol fed-batch fermentation, the process could be divided into multiple equal subintervals, and the feed operation was performed in pulse form at the start of each subinterval. Based on the macrokinetic models, the multipulse feed strategy for both glucose and corn steep slurry was determined by a general n...

journal_title：Applied biochemistry and biotechnology

authors： Xie D,Liu D,Zhu H,Liu T

更新日期：2001-08-01 00:00:00

abstract：:Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced an appreciable level of xylanase ac...

journal_title：Applied biochemistry and biotechnology

authors： Mamo G,Gessesse A

更新日期：2000-05-01 00:00:00

abstract：:Arabidopsis transcription factor APETALA2 (AP2) controls multiple aspects of plant growth and development, including seed development, stem cell maintenance, and specification of floral organ identity. Based on sequence similar of Arabidopsis AP2 and its homologues genes from other plant species, degenerate RT-PCR and...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Jiang J,Yang Y,Li P,Chen S,Zhang Z

更新日期：2012-09-01 00:00:00

abstract：:Internal air circulation affects the temperature field distribution in a gas double-dynamic solid-state fermentation bioreactor (GDSFB). To enhance heat transfer through strengthening internal air circulation in a GDSFB, we put an air distribution plate (ADP) into the bioreactor and studied the effects of forced inter...

journal_title：Applied biochemistry and biotechnology

authors： Chen H,Qin L,Li H

更新日期：2014-02-01 00:00:00

abstract：:The thermal stability of most nitrile hydratases (NHase) is poor, which has been enhanced to some extent by molecular modifications in several specific regions of the C-terminal domain (C-domain) of β subunit of NHase. Since the C-domain could be present as a naturally separate domain in a few NHases, the whole C-doma...

journal_title：Applied biochemistry and biotechnology

authors： Sun W,Zhu L,Chen X,Wu L,Zhou Z,Liu Y

更新日期：2016-04-01 00:00:00

abstract：:The enzyme 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGR) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. This enzyme has three domains: transmembrane, binding, and soluble. In this study, we expressed and puri...

journal_title：Applied biochemistry and biotechnology

authors： Andrade-Pavón D,Cuevas-Hernández RI,Trujillo-Ferrara JG,Hernández-Rodríguez C,Ibarra JA,Villa-Tanaca L

更新日期：2017-08-01 00:00:00

abstract：:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was a...

journal_title：Applied biochemistry and biotechnology

authors： Lianghua T,Liming X,Min S,Huaying G

更新日期：2007-08-01 00:00:00

abstract：:This work aimed at setting up a fully instrumented, laboratory-scale bioreactor enabling anaerobic valorization of solid substrates through hydrogen and/or volatile fatty acid (VFA) production using mixed microbial populations (consortia). The substrate used was made of meat-based wastes, especially from slaughterhous...

journal_title：Applied biochemistry and biotechnology

authors： Pessiot J,Nouaille R,Jobard M,Singhania RR,Bournilhas A,Christophe G,Fontanille P,Peyret P,Fonty G,Larroche C

更新日期：2012-07-01 00:00:00

abstract：:Lupulones, naturally produced by glandular trichomes of hop (Humulus lupulus), are prenylated phloroglucinol derivatives that contribute the bitter flavor of beer and demonstrate antimicrobial and anticancer activities. It is appealing to develop microbial cell factories such that lupulones may be produced via ferment...

journal_title：Applied biochemistry and biotechnology

authors： Guo X,Shen H,Liu Y,Wang Q,Wang X,Peng C,Liu W,Zhao ZK

更新日期：2019-07-01 00:00:00

abstract：:The effects of soybean and castorbean meals were evaluated separately, and in combinations at different ratios, as substrates for lipase production by Botryosphaeria ribis EC-01 in submerged fermentation using only distilled water. The addition of glycerol analytical grade (AG) and glycerol crude (CG) to soybean and c...

journal_title：Applied biochemistry and biotechnology

authors： Andrade MM,Barbosa AM,Bofinger MR,Dekker RF,Messias JM,Guedes CL,Zaminelli T,de Oliveira BH,de Lima VM,Dall'antonia LH

更新日期：2013-08-01 00:00:00

abstract：:Our laboratory has recently developed a device employing immobilized F0F1 adenosine triphosphatase (ATPase) that allows synthesis of adenosine triphosphate (ATP) from adenosine 5'-diphosphate and inorganic phosphate using solar energy. We present estimates of total solar energy received by Earth's land area and demons...

journal_title：Applied biochemistry and biotechnology

authors： Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

更新日期：2005-03-01 00:00:00

abstract：:The batch culture of a newly isolated strain of a green microalga, Chlorella sorokiniana, was carried out using a conical helical tubular photobioreactor. The isolate was capable of good growth at 40 degrees C under an airstream enriched with 10% CO2. The maximum photosynthetic productivity was 34.4 g of dry biomass/(...

journal_title：Applied biochemistry and biotechnology

authors： Morita M,Watanabe Y,Saiki H

更新日期：2000-06-01 00:00:00

abstract：:The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene...

journal_title：Applied biochemistry and biotechnology

authors： Sturesson S,Tjerneld F,Johansson G

更新日期：1990-12-01 00:00:00

abstract：:For trial use in the local chemotherapy of cancer by a combination of cytosine deaminase (EC 3.5.4.1) and 5-fluorocytosine (J. Biotechnol., (1985), 2, 13-21), 40 U of partially purified cytosine deaminase was obtained from 500 g of commercial compressed bakers' yeast. The enzyme, which is unstable, was immobilized to ...

journal_title：Applied biochemistry and biotechnology

authors： Katsuragi T,Sakai T,Tonomura K

更新日期：1987-09-01 00:00:00

abstract：:In this study, the prokaryotic expression system of Escherichia coli was used to modify prolyl aminopeptidase derived from Aspergillus oryzae JN-412 (AoPAP) via random mutagenesis and site-directed saturation mutagenesis. A random mutant library with a capacity of approximately 3000 mutants was compiled using error-pr...

journal_title：Applied biochemistry and biotechnology

authors： Liu D,Zhang D,Huang Q,Gu L,Zhou N,Tian Y

更新日期：2020-08-01 00:00:00

abstract：:One novel microbial esterase PHE21 was cloned from the genome of Pseudomonas oryzihabitans HUP022 identified from the deep sea of the Western Pacific. PHE21 was heterologously expressed and functionally characterized to be a robust esterase which behaved high resistance to various metal ions, organic solvents, surfact...

journal_title：Applied biochemistry and biotechnology

authors： Wang Y,Zhang Y,Hu Y

更新日期：2016-11-01 00:00:00

abstract：:1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...

journal_title：Applied biochemistry and biotechnology

authors： Pornwongthong P,Mulchandani A,Gedalanga PB,Mahendra S

更新日期：2014-05-01 00:00:00

abstract：:A soluble glucoside 3-dehydrogenase (G3DH) was purified from a newly isolated Sphingobacterium faecium ZJF-D6 CCTCC M 2013251. The enzyme was purified to 35.71-fold with a yield of 41.91 % and was estimated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis with a molecular mass of 62 kDa. The sequences of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang JF,Yang B,Chen W,Chen J

更新日期：2014-04-01 00:00:00

abstract：:The first step involved in the degradation of phthalate isomers (phthalate, isophthalate and terephthalate) is the double hydroxylation by respective aromatic-ring hydroxylating dioxygenases. These are two component enzymes consisting of 'oxygenase' and 'reductase' components. Soil isolate Pseudomonas aeruginosa strai...

journal_title：Applied biochemistry and biotechnology

authors： Karandikar R,Badri A,Phale PS

更新日期：2015-09-01 00:00:00