Enabling Heterologous Synthesis of Lupulones in the Yeast Saccharomyces cerevisiae.

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:One of the major limitations of effective nonviral gene carriers is their potential high cytotoxicity. Conjugation of polyethylene glycol (PEG) to polymers is a common approach to decrease toxicity and improve biodistribution. The aim of this study was to evaluate the effect of PEGylation on generation 5 polypropyleni...

journal_title：Applied biochemistry and biotechnology

authors： Hashemi M,Ayatollahi S,Parhiz H,Mokhtarzadeh A,Javidi S,Ramezani M

更新日期：2015-09-01 00:00:00

abstract：:Biopolymers and biopreservatives produced by microorganisms play an essential role in food technology. Polyhydroxyalkanoates and bacteriocins produced by bacteria are promising components to safeguard the environment and for food preservation applications. Polyhydroxybutyrate (PHB)-based antimicrobial films were prepa...

journal_title：Applied biochemistry and biotechnology

authors： Narayanan A,Neera,Mallesha,Ramana KV

更新日期：2013-07-01 00:00:00

abstract：:Biological control of soil-borne plant pathogens is a potential alternative to the use of chemical pesticides, which have already been proved to be harmful to the environment. Several strains of the fungus Trichoderma have been isolated and found to be effective biocontrol agents of various soil-borne plant pathogenic...

journal_title：Applied biochemistry and biotechnology

authors： Chet I,Inbar J

更新日期：1994-07-01 00:00:00

abstract：:Ovarian cancer lacks clear syndromes at an early stage and could result in serious problem in woman's health status. The current diagnostic approach relies on physical examination, ultrasound examination, and blood test for CA125. These approaches could not diagnose early stage ovarian cancer with high sensitivity and...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Y,Guo B,Bi R

更新日期：2012-10-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:A full factorial design leading to 20 sets of sorption runs was conducted to study the influence of four variables (bleaching earth and biomass concentrations, pH, and sorption time) on the iron, nickel, and chromium removal from stainless steel effluent using waste biomass from a beverage industry. Similar factor eff...

journal_title：Applied biochemistry and biotechnology

authors： Dias MA,Rosa CA,Linardi VR,Conte RA,De Castro HF

更新日期：2001-04-01 00:00:00

abstract：:This article deals with a direct electrochemical method of detecting antigens using new methods of functionalization of gold electrodes. Based on the reacting ability of gold with sulfhydryl groups, three protocols for the fixation of antibodies have been explored. They are based on either the self-assembling properti...

journal_title：Applied biochemistry and biotechnology

authors： Ameur S,Martelet C,Jaffrezic-Renault N,Chovelon JM

更新日期：2000-11-01 00:00:00

abstract：:Iminodiacetic acid (IDA) is one of the chelating ligands most frequently employed in immobilized metal-ion affinity chromatography (IMAC) due to its ability to act as electron-pair donor, forming stable complexes with intermediate and borderline Lewis metal ions (electron acceptor). Thus, IDA can also be employed in i...

journal_title：Applied biochemistry and biotechnology

authors： Bresolin ITL,Bresolin IRAP,Bueno SMA

更新日期：2020-06-01 00:00:00

abstract：:The ethanologenic bacteria Escherichia coli strains KO11 and LYO1, and Klebsiella oxytoca strain P2, were investigated for their ability to metabolize furfural. Using high performance liquid chromatography and 13C-nuclear magnetic resonance spectroscopy, furfural was found to be completely biotransformed into furfuryl...

journal_title：Applied biochemistry and biotechnology

authors： Gutiérrez T,Buszko ML,Ingram LO,Preston JF

更新日期：2002-04-01 00:00:00

abstract：:The purpose of this study was to examine the protective effects of intracellular homogeneous melanin produced by Lachnum YM156 (LIM) against ultraviolet B (UVB) induced damage in mice. The possible structural formula of the LIM was concluded based on elemental analysis, ultraviolet-visible spectroscopy (UV-Vis), Fouri...

journal_title：Applied biochemistry and biotechnology

authors： Li S,Yang L,Li J,Chen T,Ye M

更新日期：2019-06-01 00:00:00

abstract：:Ovary-specific acidic protein (OSAP) is an important reproduction-related gene. In this study, we cloned the full-length cDNA sequence of porcine OSAP gene through the rapid amplification of cDNA ends method. The porcine OSAP gene encodes a protein of 238 amino acids which shares high homology with the OSAP of five sp...

journal_title：Applied biochemistry and biotechnology

authors： Lei Y,Yonggang L

更新日期：2012-09-01 00:00:00

abstract：:The increase in waste disposal and energy costs has provided an incentive to convert carbohydrate-rich food waste streams into fuel. For example, dining halls and restaurants discard foods that require tipping fees for removal. An effective use of food waste may be the enzymatic hydrolysis of the waste to simple sugar...

journal_title：Applied biochemistry and biotechnology

authors： Davis RA

更新日期：2008-03-01 00:00:00

abstract：:(R)-2-Phenoxy-propionic acid methyl ester (PPAM) is an important chiral precursor of aryloxy phenoxy propionate herbicides. The covalent immobilization of lipase from Aspergillus oryzae WZ007 and the catalysis of enantioselective (R, S)-PPAM resolution by the immobilized A. oryzae lipase (AOL) were investigated in thi...

journal_title：Applied biochemistry and biotechnology

authors： Zhong W,Zhang M,Li X,Zhang Y,Wang Z,Zheng J

更新日期：2020-03-01 00:00:00

abstract：:A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protecti...

journal_title：Applied biochemistry and biotechnology

authors： Marques ET Jr,Lima Filho JL

更新日期：1992-01-01 00:00:00

abstract：:Bacillus amyloliquefaciens K103 isolated from a lemon sample was used as a biocontrol agent to suppress Rhizoctonia solani Kühn and other fungal plant pathogens. Two antifungal compounds were purified from the culture broth using acid precipitation, gel permeation chromatography, and reversed-phase high-performance li...

journal_title：Applied biochemistry and biotechnology

authors： Zhang B,Dong C,Shang Q,Cong Y,Kong W,Li P

更新日期：2013-12-01 00:00:00

abstract：:Penicillin V acylase (PVA) is a pharmaceutically important enzyme as it plays a vital role in the manufacture of semi-synthetic beta-lactam antibiotics. Rhodotorula aurantiaca (NCIM 3425) produced high levels of intracellular penicillin V acylase after 18 h at pH 8.0 and temperature 27 degrees C. Fructose was the best...

journal_title：Applied biochemistry and biotechnology

authors： Kumar A,Singh S,Poddar P,Prabhune A,Pundle A

更新日期：2009-06-01 00:00:00

abstract：:The effect of the addition of the nonionic surfactant tributylphenyltetraethoxylate to culture media on pH and extracellular protein content, and on production of beta-glucosidase, xylanase, laccase, and manganese-dependent and -independent peroxidases by the edible fungus Pleurotus ostreatus was determined. The relat...

journal_title：Applied biochemistry and biotechnology

authors： Ramos C,Morais H,Forgács E,Cserháti T

更新日期：2003-07-01 00:00:00

abstract：:Solid-state fermentation using the microfungus Penicillium brevicompactum for the production of mycophenolic acid is reported in this paper. Of the initial substrates tested (whole wheat, cracked wheat, long grain Basmati rice, and short grain Parmal rice), Parmal rice proved to be the best. Under initial conditions, ...

journal_title：Applied biochemistry and biotechnology

authors： Patel G,Patil MD,Soni S,Chisti Y,Banerjee UC

更新日期：2017-05-01 00:00:00

abstract：:In order to detect low levels of enzyme activity, specifically glucose oxidase, in biological samples, an immunoenzymatic assay was developed since currently available methods could not be used because of either their lack of sensitivity or the conditions prevailing in our samples: turbidity of the medium, presence of...

journal_title：Applied biochemistry and biotechnology

authors： de Bellefontaine C,Josse F,Domurado M,Domurado D

更新日期：1994-08-01 00:00:00

abstract：:Arabidopsis transcription factor APETALA2 (AP2) controls multiple aspects of plant growth and development, including seed development, stem cell maintenance, and specification of floral organ identity. Based on sequence similar of Arabidopsis AP2 and its homologues genes from other plant species, degenerate RT-PCR and...

journal_title：Applied biochemistry and biotechnology

authors： Liu Z,Gu C,Chen F,Jiang J,Yang Y,Li P,Chen S,Zhang Z

更新日期：2012-09-01 00:00:00

abstract：:Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Ps...

journal_title：Applied biochemistry and biotechnology

authors： Lee SA,Wrona LJ,Cahoon AB,Crigler J,Eiteman MA,Altman E

更新日期：2016-01-01 00:00:00

abstract：:A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed th...

journal_title：Applied biochemistry and biotechnology

authors： Fukuhara Y,Horii S,Matsuno T,Matsumiya Y,Mukai M,Kubo M

更新日期：2013-05-01 00:00:00

abstract：:In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus repo...

journal_title：Applied biochemistry and biotechnology

authors： Liu Y,Yang SX,Cheng Y,Liu DQ,Zhang Y,Deng KJ,Zheng XL

更新日期：2015-12-01 00:00:00

abstract：:Indole-3-carbinol (I3C) is a naturally occurring glucosinolate found in Brassica vegetables that is usually converted in gastric acidic environment to the efficient metabolite 3,3'-diindolylmethane (DIM). Both indoles (I3C and DIM) are known chemopreventive agents for various cancers including breast cancer. This stud...

journal_title：Applied biochemistry and biotechnology

authors： El-Daly SM,Gamal-Eldeen AM,Gouhar SA,Abo-Elfadl MT,El-Saeed G

更新日期：2020-12-01 00:00:00

abstract：:MCM-41 and MCM-48 with niobium were successfully synthesized using 1-tetradecyl-3-methylimidazolium chloride ([C14MI]Cl) as a structure-directing agent. The best Si/Nb molar ratio was chosen (Si/Nb = 20) and the CALB enzyme was immobilized in situ in the synthesized Nb-MCM. SEM micrographs showed the formation of very...

journal_title：Applied biochemistry and biotechnology

authors： Bordin I,de Aguiar Pedott V,Demaman Oro CE,Junges A,Dallago RM,Mignoni ML

更新日期：2021-01-06 00:00:00

abstract：:Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of ...

journal_title：Applied biochemistry and biotechnology

authors： Jia J,Chen H,Wu B,Cui F,Fang H,Wang H,Ni Z

更新日期：2019-01-01 00:00:00

abstract：:Urease has been immobilized and layered onto the walls of manufactured silicon microchannels. Enzyme immobilization was performed using layer-by-layer nano self-assembly. Alternating layers of oppositely charged polyelectrolytes, with enzyme layers "encased" between them, were deposited onto the walls of the silicon m...

journal_title：Applied biochemistry and biotechnology

authors： Forrest SR,Elmore BB,Palmer JD

更新日期：2005-04-01 00:00:00

abstract：:Five autotrophic strains isolated from non-photosynthetic microbial communities (NPMCs), which were screened from oceans with high CO2 fixing capability, were identified as Ochrobactrum sp. WH-2, Stenotrophomonas sp. WH-11, Ochrobactrum sp. WH-13, Castellaniella sp. WH-14, and Sinomicrobium oceani WH-15. The CO2 fixat...

journal_title：Applied biochemistry and biotechnology

authors： Hu J,Wang L,Zhang S,Xi X,Le Y,Fu X,Tsang Y,Gao M

更新日期：2015-07-01 00:00:00

abstract：:The article describes preparation of new calixarene biopolymers consisting of the immobilization of convenience calixarene derivative onto cellulose and chitosan biopolymers, and the encapsulation of these calixarene biopolymers with Candida rugosa lipase within a chemical inert sol-gel supported by polycondensation w...

journal_title：Applied biochemistry and biotechnology

authors： Ozyilmaz E,Sayin S

更新日期：2013-08-01 00:00:00