Enantioselective Resolution of (R, S)-2-Phenoxy-Propionic Acid Methyl Ester by Covalent Immobilized Lipase from Aspergillus oryzae.

Abstract:

:(R)-2-Phenoxy-propionic acid methyl ester (PPAM) is an important chiral precursor of aryloxy phenoxy propionate herbicides. The covalent immobilization of lipase from Aspergillus oryzae WZ007 and the catalysis of enantioselective (R, S)-PPAM resolution by the immobilized A. oryzae lipase (AOL) were investigated in this study. The primary amino resin LX-1000HA was selected as the support for the covalent immobilization of AOL. The Km and Vmax of the immobilized lipase were 1.97 mM and 4.84 × 103 μmol/mg min, respectively. The key reaction parameters (pH, temperature, rotation speed, and substrate concentration) for the lipase-catalyzed resolution of (R, S)-PPAM were optimized. An e.e.s of 99.5% and conversion rate of 50.8% were achieved under the optimal conditions of pH 7.5, 30 °C, and substrate concentration 500 mM. The immobilized lipase retained 87.3% of its initial activity after 15 cycles of the repeated experiments. The results demonstrated that the covalent immobilized AOL has potential industrial applications.

authors

Zhong W,Zhang M,Li X,Zhang Y,Wang Z,Zheng J

doi

10.1007/s12010-019-03145-4

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

1049-1059

issue

3

eissn

0273-2289

issn

1559-0291

pii

10.1007/s12010-019-03145-4

journal_volume

190

pub_type

杂志文章
  • Development of DNA-designed avian IgY antibodies for detection of Mycobacterium avium subsp. paratuberculosis heat shock protein 70 (Hsp70) and anti-Hsp70 antibodies in the serum of normal cattle.

    abstract::Mycobacterium avium subsp. paratuberculosis heat shock protein 70 (MAPHsp70) is an immunodominant antigen, which can be used as a subunit vaccine against bovine paratuberculosis. In the present study, we evaluated the immunogenic activities of MAPHsp70 expressed by DNA vaccine in chicken and the use of prepared specif...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9648-1

    authors: Brujeni GN,Gharibi D

    更新日期:2012-05-01 00:00:00

  • The effect of iron and copper as an essential nutrient on mitochondrial electron transport system and lipid peroxidation in Trichoderma harzianum.

    abstract::Iron and copper are essential nutrients for all living organisms as cofactors of many enzymes and play important roles in electron transport system (ETS) enzymes which have heme and iron-sulfur centers. In the present study, ETS enzymes, namely, succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), activities ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0273-4

    authors: Tavsan Z,Ayar Kayali H

    更新日期:2013-08-01 00:00:00

  • Cloning, expression, and characterization of a milk-clotting aspartic protease gene (Po-Asp) from Pleurotus ostreatus.

    abstract::An aspartic protease gene from Pleurotus ostreatus (Po-Asp) had been cloned based on the 3' portion of cDNA in our previous work. The Po-Asp cDNA contained 1,324 nucleotides with an open reading frame (ORF) of 1,212 bp encoding 403 amino acid residues. The putative amino acid sequence included a signal peptide, an act...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0674-4

    authors: Yin C,Zheng L,Chen L,Tan Q,Shang X,Ma A

    更新日期:2014-02-01 00:00:00

  • Liquefaction of corn stover and preparation of polyester from the liquefied polyol.

    abstract::This research investigated a novel process to prepare polyester from corn stover through liquefaction and crosslinking processes. First, corn stover was liquefied in organic solvents (90 wt% ethylene glycol and 10 wt% ethylene carbonate) with catalysts at moderate temperature under atmospheric pressure. The effect of ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:130:1:574

    authors: Yu F,Liu Y,Pan X,Lin X,Liu C,Chen P,Ruan R

    更新日期:2006-04-01 00:00:00

  • Stabilization of the cellulase enzyme complex as enzyme nanoparticle.

    abstract::The native Celluclast BG cellulase enzyme complex consists of different enzymes which can also degrade great substrate molecules as native celluloses. This enzyme complex has been covered by a very thin, a few nanometers thick, polymer layer, in order to improve its stability. It has been proved that the polymer layer...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9863-9

    authors: Hegedüs I,Hancsók J,Nagy E

    更新日期:2012-11-01 00:00:00

  • Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol.

    abstract::Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:84-86:1-9:69

    authors: Bollók M,Réczey K,Zacchi G

    更新日期:2000-04-01 00:00:00

  • Effective purification of Cerrena unicolor laccase using microfiltration, ultrafiltration and acetone precipitation.

    abstract::Microfiltration followed by concentration and diafiltration on ultrafiltration membranes (Biomax-100, Biomax-10 and Ultracel-10) was used to recover extracellular laccase (EC 1.10.3.2) from culture broth of wood-rotting fungus Cerrena unicolor. Feed, permeate, retentate and membrane wash-out solutions were analysed fo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8791-9

    authors: Bryjak J,Rekuć A

    更新日期:2010-04-01 00:00:00

  • Simultaneous Wastewater Treatment, Algal Biomass Production and Electricity Generation in Clayware Microbial Carbon Capture Cells.

    abstract::Performance of microbial carbon capture cells (MCCs), having a low-cost clayware separator, was evaluated in terms of wastewater treatment and electricity generation using algae Chlorella pyrenoidosa in MCC-1 and Anabaena ambigua in MCC-2 and without algae in a cathodic chamber of MCC-3. Higher power production was ac...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2485-5

    authors: Jadhav DA,Jain SC,Ghangrekar MM

    更新日期:2017-11-01 00:00:00

  • Core-Shell Encapsulation of Lipophilic Substance in Jelly Fig (Ficus awkeotsang Makino) Polysaccharides Using an Inexpensive Acrylic-Based Millifluidic Device.

    abstract::The polysaccharides extracted from the achenes of jelly fig, Ficus awkeotsang Makino, were mainly composed of low methyl pectin and used as a novel shell material for encapsulating lipophilic bioactives in the core of microcapsule. The polysaccharide microcapsules with oil core were prepared using a novel acrylic-base...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03209-5

    authors: Ponrasu T,Yang RF,Chou TH,Wu JJ,Cheng YS

    更新日期:2020-05-01 00:00:00

  • Rapid Simultaneous Detection of Anti-protozoan Drugs Using a Lateral-Flow Immunoassay Format.

    abstract::This research describes the development of a multi-analyte lateral-flow immunoassay intended for the simultaneous detection of three anti-protozoan drugs (coccidiostats). These drugs, namely, halofuginone, toltrazuril and diclazuril, are used in the treatment of Eimeria spp. infections in cattle, pigs, chickens and tu...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1582-6

    authors: Fitzgerald J,Leonard P,Danaher M,O'Kennedy R

    更新日期:2015-05-01 00:00:00

  • Molecular modification of Protein A to improve the elution pH and alkali resistance in affinity chromatography.

    abstract::Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mut...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0818-1

    authors: Xia HF,Liang ZD,Wang SL,Wu PQ,Jin XH

    更新日期:2014-04-01 00:00:00

  • Functional validation of Phragmites communis glutathione reductase (PhaGR) as an essential enzyme in salt tolerance.

    abstract::Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown. In the present study, we cl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1514-5

    authors: Zhang X,Quan G,Wang J,Han H,Chen S,Guo S,Yin H

    更新日期:2015-04-01 00:00:00

  • Characteristics of White-rot Fungus Phlebia brevispora TMIC33929 and Its Growth-Promoting Bacterium Enterobacter sp. TN3W-14 in the Decolorization of Dye-Contaminated Water.

    abstract::The dye decolorization potential of the white-rot fungus Phlebia brevispora TMIC33929 when grown alone or in coculture with its growth-promoting bacterium Enterobacter sp. TN3W-14 was evaluated in low nitrogen liquid medium at different pHs. Axenic fungus removed a similar amount of Congo red and crystal violet at pH ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03062-6

    authors: Harry-Asobara JL,Kamei I

    更新日期:2019-12-01 00:00:00

  • Immobilized carboxypeptidase N. A potent bioreactor and specific adsorbent for peptides.

    abstract::Carboxypeptidase N-Sepharose was prepared by covalent immobilization of purified human plasma carboxypeptidase N. More than 98% of the carboxypeptidase N was immobilized; 42% of the applied activity can be detected on the support. The column has excellent capabilities to quantitatively remove carboxy-terminal basic am...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02921652

    authors: Wang W,Hendriks DF,Scharpé SL

    更新日期:1994-02-01 00:00:00

  • A diverse assemblage of indole-3-acetic acid producing bacteria associate with unicellular green algae.

    abstract::Microalgae have tremendous potential as a renewable feedstock for the production of liquid transportation fuels. In natural waters, the importance of physical associations and biochemical interactions between microalgae and bacteria is generally well appreciated, but the significance of these interactions to algal bio...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0980-5

    authors: Bagwell CE,Piskorska M,Soule T,Petelos A,Yeager CM

    更新日期:2014-08-01 00:00:00

  • Biotechnological storage and utilization of entrapped solar energy.

    abstract::Our laboratory has recently developed a device employing immobilized F0F1 adenosine triphosphatase (ATPase) that allows synthesis of adenosine triphosphate (ATP) from adenosine 5'-diphosphate and inorganic phosphate using solar energy. We present estimates of total solar energy received by Earth's land area and demons...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:120:3:159

    authors: Bhattacharya S,Schiavone M,Nayak A,Bhattacharya SK

    更新日期:2005-03-01 00:00:00

  • Olive Recombinant Hydroperoxide Lyase, an Efficient Biocatalyst for Synthesis of Green Leaf Volatiles.

    abstract::Volatile C6-aldehydes are the main contributors to the characteristic odor of plants known as "green note" and are widely used by the flavor industry. Biotechnological processes were developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants. Recombinant hydroperoxide lyases (HPLs) constit...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2023-x

    authors: Jacopini S,Mariani M,de Caraffa VB,Gambotti C,Vincenti S,Desjobert JM,Muselli A,Costa J,Berti L,Maury J

    更新日期:2016-06-01 00:00:00

  • Determination of cyanide using a microbial sensor.

    abstract::A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrod...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02788064

    authors: Nakanishi K,Ikebukuro K,Karube I

    更新日期:1996-08-01 00:00:00

  • Effect of extruder parameters and moisture content of switchgrass, prairie cord grass on sugar recovery from enzymatic hydrolysis.

    abstract::Research on biomass pretreatment to enhance enzymatic digestibility has been done for more than decades, but a viable continuous pretreatment method needs to be developed. Extrusion has the potential to be a viable continuous pretreatment method. This study investigated the effect of compression ratio (2:1 and 3:1), s...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-8959-3

    authors: Karunanithy C,Muthukumarappan K

    更新日期:2010-11-01 00:00:00

  • Apoptin as a potential viral gene oncotherapeutic agent.

    abstract::The use of viruses for treatment of cancer overcomes the bottlenecks of chemotherapy and radiotherapy. Several viruses and their proteins have been evaluated for oncolytic effect. The VP3 protein (apoptin) of chicken anemia virus is one such protein with an inherent ability to lyse cancer and transformed cells while l...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1567-5

    authors: Singh PK,Tiwari AK,Rajmani RS,Kumar GR,Chaturvedi U,Saxena L,Saxena S,Doley J,Sahoo AP,Santra L,Saxena M,Kumar S,Sharma B

    更新日期:2015-05-01 00:00:00

  • Observation of geometric structure of collagen molecules by atomic force microscopy.

    abstract::Atomic force microscopy was used to study the geometric structure of collagen fibrils and molecules of rat calcanean tendon tissues. The authors found that the diameter of the fibrils ranged from 124 to 170 nm, and their geometric form suggested a helical winding with spectral period from 59.4 to 61.7 nm, close to the...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02919391

    authors: Baranauskas V,Vidal BC,Parizotto NA

    更新日期:1998-02-01 00:00:00

  • The slope method: a tool for analyzing semi-continuous data.

    abstract::The MixAlco process is a biorefinery that converts lignocellulose into useful chemicals and hydrocarbon fuels via mixed-acid fermentation. For a semi-continuous-staged fermentation train, during each transfer, discrete amounts of material are moved between fermentors and data are tabulated. Because of natural day-to-d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9086-x

    authors: Smith AD,Holtzapple MT

    更新日期:2011-04-01 00:00:00

  • A novel immobilized-enzyme system utilizing microcapsules.

    abstract::A novel immobilized-enzyme system that has glucoamylase on the surface of, and glucose oxidase within, polyurea microcapsules was developed. This system was found to carry out its sequential enzymatic reaction effectively. It was also demonstrated that the use of microcapsules was useful for this immobilized-enzyme sy...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02798496

    authors: Komori T,Muramatsu N,Kondo T

    更新日期:1987-02-01 00:00:00

  • Prospecting Agro-waste Cocktail: Supplementation for Cellulase Production by a Newly Isolated Thermophilic B. licheniformis 2D55.

    abstract::Bacteria isolated from thermophilic environment that can produce cellulase as well as utilise agro-waste biomass have a high potential for developing thermostable cellulase required in the biofuel industry. The cost for cellulase represents a significant challenge in converting lignocellulose to fermentable sugars for...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2401-z

    authors: Kazeem MO,Shah UKM,Baharuddin AS,AbdulRahman NA

    更新日期:2017-08-01 00:00:00

  • Molecular characterization of a gene for aldose reductase ( CbXYL1) from Candida boidinii and its expression in Saccharomyces cerevisiae.

    abstract::Candida boidinii produces significant amounts of xylitol from xylose, and assays of crude homogenates for aldose (xylose) reductase (XYL1p) have been reported to show relatively high activity with NADH as a cofactor even though XYL1p purified from this yeast does not have such activity. A gene coding for XYL1p from C....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:106:1-3:265

    authors: Kang MH,Ni H,Jeffries TW

    更新日期:2003-04-01 00:00:00

  • In Vivo Evaluation of the Toxic Effect of Ethyl Acetate Extracts of Marine Antibiotic Resistance Pseudomonas Species Derived from the Red Sea.

    abstract::Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2553-x

    authors: El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

    更新日期:2018-01-01 00:00:00

  • Effect of ceramide on mesenchymal stem cell differentiation toward adipocytes.

    abstract::Proinflammatory cytokines such as tumor necrosis factor (TNF) alpha are well known to inhibit adipocyte differentiation. TNF-alpha triggers ceramide synthesis through binding of TNF-alpha to its p55 receptor. Therefore, ceramide is implicated in many of the multiple signaling pathways initiated by TNF-alpha. In breast...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8505-8

    authors: Xu F,Yang CC,Gomillion C,Burg KJ

    更新日期:2010-01-01 00:00:00

  • Engineering Pichia pastoris for Efficient Production of a Novel Bifunctional Strongylocentrotus purpuratus Invertebrate-Type Lysozyme.

    abstract::Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2753-z

    authors: Huang P,Shi J,Sun Q,Dong X,Zhang N

    更新日期:2018-10-01 00:00:00

  • Cloning, sequence, and association analysis of porcine OSAP gene.

    abstract::Ovary-specific acidic protein (OSAP) is an important reproduction-related gene. In this study, we cloned the full-length cDNA sequence of porcine OSAP gene through the rapid amplification of cDNA ends method. The porcine OSAP gene encodes a protein of 238 amino acids which shares high homology with the OSAP of five sp...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9788-3

    authors: Lei Y,Yonggang L

    更新日期:2012-09-01 00:00:00

  • Reduction of Cr(6+) to Cr(3 +) in a packed-bed bioreactor.

    abstract::Hexavalent chromium, Cr(6+), is a common and toxic pollutant in soils and waters. Reduction of the mobile Cr(6+) to the less mobile and less toxic trivalent chromium, Cr(3+), can be achieved with conventional chemical reduction technologies. Alternatively, Cr(6+) can be biochemically reduced to Cr(3+) by anaerobic mic...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/978-1-4612-2312-2_77

    authors: Turick CE,Camp CE,Apel WA

    更新日期:1997-04-01 00:00:00