Abstract:
:A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrode. When cyanide is added to the sample solution, the electron transport chain reaction of the respiration system in the mitochondria is inhibited, resulting in a decrease in respiration. The inhibition is caused by cyanide binding with respiration enzymes such as the cytochrome oxidase complex in the mitochondrial inner membrane. Therefore, the cyanide concentration can be measured from the change in the respiration rate. When the sensor was applied to a batch system at pH 8.0 and 30 degrees C, the cyanide calibration curve showed linearity in the concentration range between 0.3 microM and 150 microM CN-.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Nakanishi K,Ikebukuro K,Karube Idoi
10.1007/BF02788064subject
Has Abstractpub_date
1996-08-01 00:00:00pages
97-106issue
2eissn
0273-2289issn
1559-0291journal_volume
60pub_type
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