Determination of cyanide using a microbial sensor.

Abstract:

:A microbial cyanide sensor was prepared, consisting of immobilized Saccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of the microorganisms increased. The change in the respiration activity is monitored with the oxygen electrode. When cyanide is added to the sample solution, the electron transport chain reaction of the respiration system in the mitochondria is inhibited, resulting in a decrease in respiration. The inhibition is caused by cyanide binding with respiration enzymes such as the cytochrome oxidase complex in the mitochondrial inner membrane. Therefore, the cyanide concentration can be measured from the change in the respiration rate. When the sensor was applied to a batch system at pH 8.0 and 30 degrees C, the cyanide calibration curve showed linearity in the concentration range between 0.3 microM and 150 microM CN-.

authors

Nakanishi K,Ikebukuro K,Karube I

doi

10.1007/BF02788064

subject

Has Abstract

pub_date

1996-08-01 00:00:00

pages

97-106

issue

2

eissn

0273-2289

issn

1559-0291

journal_volume

60

pub_type

杂志文章
  • Gelatin-immobilised poly(hydroxyethyl methacrylate) cryogel for affinity purification of fibronectin.

    abstract::In this work, fibronectin purification from human plasma with the gelatin-immobilised poly(hydroxyethyl methacrylate) (PHEMA) cryogel has been evaluated. The PHEMA cryogel was prepared by cryo-polymerisation which proceeds in an aqueous solution of monomer frozen inside a plastic syringe. The PHEMA cryogel contained i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0352-6

    authors: Perçin I,Aksöz E,Denizli A

    更新日期:2013-09-01 00:00:00

  • Biotechnological production of xylitol from agroindustrial residues. Evaluation of bioprocesses.

    abstract::Batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. The best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920197

    authors: Rodrigues DC,Silva SS,Prata AM,Felipe MG

    更新日期:1998-04-01 00:00:00

  • New Insights on Coffea miRNAs: Features and Evolutionary Conservation.

    abstract::Small RNAs influence the gene expression at the post-transcriptional level by guiding messenger RNA (mRNA) cleavage, translational repression, and chromatin modifications. In addition to model plants, the microRNAs (miRNAs) have been identified in different crop species. In this work, we developed a specific pipeline ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1785-x

    authors: Chaves SS,Fernandes-Brum CN,Silva GF,Ferrara-Barbosa BC,Paiva LV,Nogueira FT,Cardoso TC,Amaral LR,de Souza Gomes M,Chalfun-Junior A

    更新日期:2015-10-01 00:00:00

  • Saccharification of marine microalgae using marine bacteria for ethanol production.

    abstract::The saccharification of marine microalgae using amylase from marine bacteria in saline conditions was investigated. An amylase-producing bacterium, Pseudoalterimonas undina NKMB 0074 was isolated and identified. The green microalga NKG 120701 was determined to have the highest concentration of intracellular carbohydra...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:105:1-3:247

    authors: Matsumoto M,Yokouchi H,Suzuki N,Ohata H,Matsunaga T

    更新日期:2003-04-01 00:00:00

  • Effect of extruder parameters and moisture content of switchgrass, prairie cord grass on sugar recovery from enzymatic hydrolysis.

    abstract::Research on biomass pretreatment to enhance enzymatic digestibility has been done for more than decades, but a viable continuous pretreatment method needs to be developed. Extrusion has the potential to be a viable continuous pretreatment method. This study investigated the effect of compression ratio (2:1 and 3:1), s...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-8959-3

    authors: Karunanithy C,Muthukumarappan K

    更新日期:2010-11-01 00:00:00

  • A new method for microcapsule characterization: use of fluorogenic genipin to characterize polymeric microcapsule membranes.

    abstract::Numerous microcapsule systems have been developed for a wide range of applications, including the sustained release of drugs, cell transplantation for therapy, cell immobilization, and other biotechnological applications. Despite the fact that microcapsule membrane is a dominant factor governing overall microcapsule p...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:134:3:207

    authors: Chen H,Ouyang W,Lawuyi B,Lim T,Prakash S

    更新日期:2006-09-01 00:00:00

  • Interrelations of Apoptotic and Cellular Senescence Genes Methylation in Inflammatory Bowel Disease Subtypes and Colorectal Carcinoma in Egyptians Patients.

    abstract::Ras-related domain family member 1 transcript variant A (RASSF1A) controls apoptosis and cell proliferation while p14/ARF gene has a regulatory role in cellular senescence. Failure of apoptosis and cellular senescence occurs during inflammatory bowel disease (IBD) and colorectal cancer (CRC). To reveal the role of per...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03017-x

    authors: Salama RH,Sayed ZEA,Ashmawy AM,Elsewify WA,Ezzat GM,Mahmoud MA,Alsanory AA,Alsanory TA

    更新日期:2019-09-01 00:00:00

  • Conversion of isoeugenol to vanillin by Psychrobacter sp. strain CSW4.

    abstract::To screen strains of halotolerant or halophile bacteria which are able to convert isoeugenol to vanillin, 36 different strains of bacteria isolated from the salty environments in Iran were investigated. During growth on isoeugenol, a moderately halotolerant Gram-negative coccobacil showed capability of converting isoe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9397-6

    authors: Ashengroph M,Nahvi I,Zarkesh-Esfahani H,Momenbeik F

    更新日期:2012-01-01 00:00:00

  • Production of fumaric acid from L-malic acid by solvent engineering using a recombinant thermostable fumarase from Thermus thermophilus HB8.

    abstract::Currently, fumaric acid is produced by catalytic isomerization of maleic acid in aqueous solutions at low pH. Being petroleum based, requiring catalyst, and producing vast amounts of by-products and wastewater, the production of fumaric acid from renewable resources by a "green" process is increasingly attractive. In ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1468-z

    authors: Liu Y,Song J,Tan T,Liu L

    更新日期:2015-03-01 00:00:00

  • Maximum production of fermentable sugars from barley straw using optimized soaking in aqueous ammonia (SAA) pretreatment.

    abstract::Soaking in aqueous ammonia (SAA) pretreatment was investigated to improve enzymatic digestibility and consequently to increase total fermentable sugar production from barley straw. Various effects of pretreatment process parameters, such as reaction temperature, reaction time, solid:liquid ratio, and ammonia concentra...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0154-x

    authors: Yoo CG,Nghiem NP,Hicks KB,Kim TH

    更新日期:2013-04-01 00:00:00

  • White rot fungus Inonotus obliquus pretreatment to improve tran-1,4-polyisoprene extraction and enzymatic saccharification of Eucommia ulmoides leaves.

    abstract::This study proposes an innovative strategy of lignocellulose biodegradation by Inonotus obliquus under solid-state fermentation in extracting Eucommia ulmoides trans-1,4-polyisoprene (EUG) and producing reducing sugars efficiently. EUG and sugars were obtained through the white rot fungal pretreatment of E. ulmoides l...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03347-1

    authors: Qian S,Zhang C,Zhu Z,Huang P,Xu X

    更新日期:2020-11-01 00:00:00

  • Optimization of fermentation conditions for production of glycopeptide antibiotic vancomycin by Amycolatopsis orientalis.

    abstract::Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:102-103:1-6:395

    authors: Padma PN,Rao AB,Yadav JS,Reddy G

    更新日期:2002-07-01 00:00:00

  • Immobilization of L-Asparaginase on Magnetic Nanoparticles for Cancer Treatment.

    abstract::In this presented work, magnetic poly(HEMA-GMA) nanoparticles were synthesized, characterized, and used for immobilization of an anti-leukemic enzyme L-asparaginase. The average particle size of the synthesized magnetic nanoparticles was found to be as 117.5 nm. L-asparaginase was successfully immobilized onto the syn...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03276-z

    authors: Orhan H,Aktaş Uygun D

    更新日期:2020-08-01 00:00:00

  • Production of L-malic acid via biocatalysis employing wild-type and respiratory-deficient yeasts.

    abstract::The yeast Saccharomyces cerevisiae has been used to efficiently produce L-malic acid from fumaric acid. Fumarase is responsible for the reversible conversion of fumaric and L-malic acids in the TCA cycle. To investigate the function of mitochondrial and cytoplasmic fumarase isoenzymes in L-malic acid bioconversion, a ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920194

    authors: Wang X,Gong CS,Tsao GT

    更新日期:1998-04-01 00:00:00

  • Organic and Inorganic Nitrogen Impact Chlorella variabilis Productivity and Host Quality for Viral Production and Cell Lysis.

    abstract::Microalgae have been proposed as a potential feedstock for biofuel production; however, cell disruption is usually required for collection and utilization of cytoplasmic polysaccharides and lipids. Virus infection might be one approach to disrupt the cell wall. The concentration of yeast extract and presence of KNO3 i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1588-0

    authors: Cheng YS,Labavitch J,VanderGheynst JS

    更新日期:2015-05-01 00:00:00

  • A novel beta-mannanase with high specific activity from Bacillus circulans CGMCC1554: gene cloning, expression and enzymatic characterization.

    abstract::A DNA fragment of 2,042 bp containing a novel beta-mannanase gene, man5A, was identified from the genome of the mannan-degrading bacterium Bacillus circulans CGMCC1554. The open reading frame of man5A comprised 978 bp encoding a protein of 326 amino acids with a predicted molecular weight of 32 kDa. The amino acid seq...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8364-3

    authors: Yang P,Li Y,Wang Y,Meng K,Luo H,Yuan T,Bai Y,Zhan Z,Yao B

    更新日期:2009-10-01 00:00:00

  • Bioremediation of distillery sludge into soil-enriching material through vermicomposting with the help of Eisenia fetida.

    abstract::The aim of the present study was bioremediation of distillery sludge into a soil-enriching material. It was mixed with a complementary waste, cattle dung, and subjected to vermicomposting with (V) and without (T, control) Eisenia fetida in the ratio of 0:100 % (V1, T1), 10:90 (V2, T2), 25:75 (V3, T3), 50:50 (V4, T4), ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1116-7

    authors: Singh J,Kaur A,Vig AP

    更新日期:2014-10-01 00:00:00

  • The influence of initial xylose concentration, agitation, and aeration on ethanol production by Pichia stipitis from rice straw hemicellulosic hydrolysate.

    abstract::Rice straw hemicellulosic hydrolysate was used as fermentation medium for ethanol production by Pichia stipitis NRRL Y-7124. Shaking bath experiments were initially performed aiming to establish the best initial xylose concentration to be used in this bioconversion process. In the sequence, assays were carried out und...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8867-6

    authors: Silva JP,Mussatto SI,Roberto IC

    更新日期:2010-11-01 00:00:00

  • Novel approach of corn fiber utilization.

    abstract::The corn wet milling process produces a 10% (w/w of the processed corn) byproduct called corn fiber, which is utilized worldwide as a low-value feedstock for cattle. The aim of this study was to find a higher value use of corn fiber. The main fractions of corn fiber are: 20% starch, 40% hemicellulose, 14% cellulose, a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/ABAB:131:1:738

    authors: Kálmán G,Recseg K,Gáspár M,Réczey K

    更新日期:2006-03-01 00:00:00

  • Nb-MCM-Type Mesoporous Material Synthesis Using Ionic Solid as Structure-Directing Agent for In Situ Lipase Immobilization.

    abstract::MCM-41 and MCM-48 with niobium were successfully synthesized using 1-tetradecyl-3-methylimidazolium chloride ([C14MI]Cl) as a structure-directing agent. The best Si/Nb molar ratio was chosen (Si/Nb = 20) and the CALB enzyme was immobilized in situ in the synthesized Nb-MCM. SEM micrographs showed the formation of very...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03484-7

    authors: Bordin I,de Aguiar Pedott V,Demaman Oro CE,Junges A,Dallago RM,Mignoni ML

    更新日期:2021-01-06 00:00:00

  • The Production of High Purity Phycocyanin by Spirulina platensis Using Light-Emitting Diodes Based Two-Stage Cultivation.

    abstract::Phycocyanin is a photosynthetic pigment found in photosynthetic cyanobacteria, cryptophytes, and red algae. In general, production of phycocyanin depends mainly on the light conditions during the cultivation period, and purification of phycocyanin requires expensive and complex procedures. In this study, we propose a ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1879-5

    authors: Lee SH,Lee JE,Kim Y,Lee SY

    更新日期:2016-01-01 00:00:00

  • Chitosan-modified carbon nanotubes-based platform for low-density lipoprotein detection.

    abstract::We have fabricated an immunosensor based on carbon nanotubes and chitosan (CNT-CH) composite for detection of low density lipoprotein (LDL) molecules via electrochemical impedance technique. The CNT-CH composite deposited on indium tin oxide (ITO)-coated glass electrode has been used to covalently interact with anti-a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1179-5

    authors: Ali MA,Singh N,Srivastava S,Agrawal VV,John R,Onoda M,Malhotra BD

    更新日期:2014-10-01 00:00:00

  • Ascorbic acid biosensor using ascorbate oxidase immobilized on alkylamine glass beads.

    abstract::A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protecti...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02922149

    authors: Marques ET Jr,Lima Filho JL

    更新日期:1992-01-01 00:00:00

  • Development of a differential volume reactor system for soil biodegradation studies.

    abstract::A bench scale experimental system was developed for the analysis of polycyclic aromatic hydrocarbon (PAH) degradation by mixed microbial cultures in PAH contaminated Manufactured Gas Plant (MGP) soils and on sand. The reactor system was chosen in order to provide a fundamental protocol capable for evaluating the perfo...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02922585

    authors: Webb OF,Phelps TJ,Bienkowski PR,DiGrazia PM,Reed GD,Applegate B,White DC,Sayler GS

    更新日期:1991-04-01 00:00:00

  • Immobilization of alkaliphilic Bacillus sp. cells for xylanase production using batch and continuous culture.

    abstract::Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced an appreciable level of xylanase ac...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:87:2:95

    authors: Mamo G,Gessesse A

    更新日期:2000-05-01 00:00:00

  • Induction of a high-yield lovastatin mutant of Aspergillus terreus by ¹²C⁶⁺ heavy-ion beam irradiation and the influence of culture conditions on lovastatin production under submerged fermentation.

    abstract::Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9308-x

    authors: Li SW,Li M,Song HP,Feng JL,Tai XS

    更新日期:2011-10-01 00:00:00

  • New analytical method using coupled enzymes for determination of polyunsaturated fatty acid content in olive oil.

    abstract::A new, simple, and original method is described for specific measurement of polyunsaturated fatty acid content in olive oil. This analytical system uses coupled enzymes, lipase and lipoxygenase. The system consists of lipase-catalyzed hydrolysis of triacylglycerol and subsequent lipoxygenation of liberated polyunsatur...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-8935-y

    authors: Ben Rejeb I,Monser L,Gargouri M

    更新日期:2010-11-01 00:00:00

  • Theoretical and Instrumental Studies of the Competitive Interaction Between Aromatic α-Aminobisphosphonates with DNA Using Binding Probes.

    abstract::Fluorescence spectroscopy, UV-visible absorption spectroscopy, circular dichroism (CD) spectroscopy, viscometry, cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were applied to investigate the competitive interaction of DNA with two aromatic α-aminobisphosphonates and neutral red dye (NR, intercalato...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2371-6

    authors: Gholivand MB,Peyman H,Gholivand K,Roshanfekr H,Taherpour AA,Yaghobi R

    更新日期:2017-07-01 00:00:00

  • Isolation, characterization, and structure analysis of a vacuolar processing enzyme gene (MhVPEγ) from Malus hupehensis (Pamp) Rehd.

    abstract::Vacuolar processing enzymes (VPEs) have received considerable attention recently, as they exhibit caspase-1-like cleavage activity and regulate the process of PCD. However, knowledge about their detailed characteristics and structures is relatively limited. In this study, a gamma vacuolar processing enzyme gene, MhVPE...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0867-5

    authors: Ran K,Yang H,Sun X,Li Q,Jiang Q,Zhang W,Shen W

    更新日期:2014-05-01 00:00:00

  • Simultaneous saccharification and fermentation of cellulosic biomass to acetic acid.

    abstract::A strain of Clostridium thermoaceticum (ATCC 49707) was evaluated for its homoacetate potential. This thermophilic anaerobe best produces acetate from glucose at pH 6.0 and 59 degrees C with a yield of 83% of theoretical. Enzyme hydrolysis of two substrates, a-cellulose and a pulp mill sludge, yielded 68% and 70% dige...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:84-86:1-9:963

    authors: Borden JR,Lee YY,Yoon HH

    更新日期:2000-04-01 00:00:00