Abstract:
:The yeast Saccharomyces cerevisiae has been used to efficiently produce L-malic acid from fumaric acid. Fumarase is responsible for the reversible conversion of fumaric and L-malic acids in the TCA cycle. To investigate the function of mitochondrial and cytoplasmic fumarase isoenzymes in L-malic acid bioconversion, a wild-type strain and a cytoplasmic respiratory-deficient mutant devoid of functional mitochondria were employed. The mutant strain, which only contained the cytoplasmic fumarase, was still functional in fumaric acid to L-malic acid bioconversion However, its specific conversion rate was much lower (0.20 g/g.h) than that of the wild-type strain (0.55 g/g.h).
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Wang X,Gong CS,Tsao GTdoi
10.1007/BF02920194subject
Has Abstractpub_date
1998-04-01 00:00:00pages
845-52eissn
0273-2289issn
1559-0291journal_volume
70-72pub_type
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