Abstract:
:An extracellular polygalacturonase (PG) produced from Paecilomyces variotii was purified to homogeneity through two chromatography steps using DEAE-Fractogel and Sephadex G-100. The molecular weight of P. variotii PG was 77,300 Da by gel filtration and SDS-PAGE. PG had isoelectric point of 4.37 and optimum pH 4.0. PG was very stable from pH 3.0 to 6.0. The extent of hydrolysis of different pectins by the purified enzyme was decreased with an increase in the degree of esterification. PG had no activity toward non-pectic polysaccharides. The apparent K(m) and V(max) values for hydrolyzing sodium polypectate were 1.84 mg/mL and 432 micromol/min/mg, respectively. PG was found to have temperature optimum at 65 degrees Celsius and was totally stable at 45 degrees Celsius for 90 min. Half-life at 55 degrees Celsius was 50.6 min. Almost all the examined metal cations showed partial inhibitory effects under enzymatic activity, except for Na(+1), K(+1), and Co(+2) (1 mM) and Cu(+2) (1 and 10 mM).
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
de Lima Damásio AR,da Silva TM,Maller A,Jorge JA,Terenzi HF,Polizeli Mde Ldoi
10.1007/s12010-009-8682-0subject
Has Abstractpub_date
2010-03-01 00:00:00pages
1496-507issue
5eissn
0273-2289issn
1559-0291journal_volume
160pub_type
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