Expression and characterization of recombinant GH11 xylanase from thermotolerant Streptomyces sp. SWU10.

abstract：:Aspartate aminotransferase (AspAT, EC 2.6.1.1) was bound on CNBr-activated Sepharose and the effects of immobilization on the maximum velocity, biologically active pyridoxal-5'-phosphate (PLP), and transaminationable active centers were studied. By comparing these parameters of soluble and immobilized enzyme the facto...

journal_title：Applied biochemistry and biotechnology

authors： Kurkijärvi K,Korpela T

更新日期：1983-04-01 00:00:00

abstract：:To facilitate structural studies of receptor-interacting protein 3 (RIP3), we developed a large-scale expression system of a glutathione-S-transferase (GST) fused with an 82 amino acid RIP3 protein in Escherichia coli. RIP3 truncation was subcloned into the pGEX-4T-1 vector and overexpressed in BL21(DE3)RIL cells. The...

journal_title：Applied biochemistry and biotechnology

authors： Jeong MS,Park JS,Jang SB

更新日期：2007-05-01 00:00:00

abstract：:A novel immobilized-enzyme system that has glucoamylase on the surface of, and glucose oxidase within, polyurea microcapsules was developed. This system was found to carry out its sequential enzymatic reaction effectively. It was also demonstrated that the use of microcapsules was useful for this immobilized-enzyme sy...

journal_title：Applied biochemistry and biotechnology

authors： Komori T,Muramatsu N,Kondo T

更新日期：1987-02-01 00:00:00

abstract：:Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains...

journal_title：Applied biochemistry and biotechnology

authors： Li SW,Li M,Song HP,Feng JL,Tai XS

更新日期：2011-10-01 00:00:00

abstract：:The genes encoding purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase), and thymidine phosphorylase (TPase) from Escherichia coli K12 were cloned respectively into expression vector pET-11a or pET-28a. The recombinant plasmids were transformed into the host strain E. coli BL21(DE3) to construct fou...

journal_title：Applied biochemistry and biotechnology

authors： Ge C,OuYang L,Ding Q,Ou L

更新日期：2009-10-01 00:00:00

abstract：:The significant challenges presented by the April 20, 2010 explosion, sinking, and subsequent oil spill of the Deepwater Horizon drilling platform in Canyon Block 252 about 52 miles southeast of Venice, LA, USA greatly impacted Louisiana's coastal ecosystem including the sea food industry, recreational fishing, and to...

journal_title：Applied biochemistry and biotechnology

authors： Boopathy R,Shields S,Nunna S

更新日期：2012-07-01 00:00:00

abstract：:Nephelium lappaceum L., commonly known as "rambutan," is a typical tropical tree and is well known for its juicy and sweet fruit which has an exotic flavor. Chemical studies on rambutan have led to the identification of various components such as monoterpene lactones and volatile compounds. Here, a 22.5-kDa trypsin in...

journal_title：Applied biochemistry and biotechnology

authors： Fang EF,Ng TB

更新日期：2015-04-01 00:00:00

abstract：:The objective of this study was to evaluate the capability of a lipopolysaccharide, produced by Trichosporon mycotoxinivorans CLA2 using residue of biodiesel processing, to flocculate two different solids in suspension. In addition, the emulsifying activity and the stability of this lipopolysaccharide in response to p...

journal_title：Applied biochemistry and biotechnology

authors： Domingues VS,Monteiro AS,Ferreira GF,Santos VL

更新日期：2017-05-01 00:00:00

abstract：:This research aimed at developing and designing a slow and targeted delivery of Amphotericin B (AmB) antibiotic by placing three types of shells containing different ratios of biodegradable and biocompatible polymers poly (D, L-lactide)-co-(glycolide) (PLGA), polyethylene glycol (PEG), and polyvinyl pyrrolidone (PVP) ...

journal_title：Applied biochemistry and biotechnology

authors： Sadat Akhavi S,Moradi Dehaghi S

更新日期：2020-06-01 00:00:00

abstract：:Prevalence of heavy metals in the living environment causes chemical stress and reactive oxygen species (ROS) formation in Phanerochaete chrysosporium (P. chrysosporium). However, the mechanisms involved in ROS defense are still under investigation. In the present study, we evaluated the effect of lead- and cadmium-in...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Q,Zeng G,Chen G,Yan M,Chen A,Du J,Huang J,Yi B,Zhou Y,He X,He Y

更新日期：2015-02-01 00:00:00

abstract：:We have previously demonstrated that pretreatment of corn stover with dilute sulfuric acid can achieve high digestibility and efficient recovery of hemicellulose sugars with high yield and concentration. Further improvement of this process was sought in this work. A modification was made in the operation of the percol...

journal_title：Applied biochemistry and biotechnology

authors： Zhu Y,Lee YY,Elander RT

更新日期：2005-04-01 00:00:00

abstract：:Fresh-harvested, air-dried rice straw was pretreated at a water content of 5 g H(2)O/g straw using sodium hydroxide (NaOH) and compared to pretreatment at 10 g H(2)O/g straw by hydrated lime (Ca(OH)(2)). Full factorial experiments including parallel wash-only treatments were completed with both sources of alkali. The ...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YS,Zheng Y,Yu CW,Dooley TM,Jenkins BM,VanderGheynst JS

更新日期：2010-11-01 00:00:00

abstract：:We have fabricated an immunosensor based on carbon nanotubes and chitosan (CNT-CH) composite for detection of low density lipoprotein (LDL) molecules via electrochemical impedance technique. The CNT-CH composite deposited on indium tin oxide (ITO)-coated glass electrode has been used to covalently interact with anti-a...

journal_title：Applied biochemistry and biotechnology

authors： Ali MA,Singh N,Srivastava S,Agrawal VV,John R,Onoda M,Malhotra BD

更新日期：2014-10-01 00:00:00

abstract：:The biosurfactant-producing Pseudomonas aeruginosa A11, with plant-growth-promoting (PGP) and multi-metal-resistant (MMR) features was isolated from the rhizosphere of a wild plant Parthenium hysterophorus. The strain A11 was able to utilize glycerol as a carbon source and produce 4,436.9 mg/L of biosurfactant after 1...

journal_title：Applied biochemistry and biotechnology

authors： Singh AK,Cameotra SS

更新日期：2013-07-01 00:00:00

abstract：:The application of immunoaffinity techniques to subcellular fractionation is reviewed and the basic principles underlying the various methods that have been successfully employed, identified. The requirement for organelle-specific antigens and high-avidity antibodies is discussed, as is the widespread use of indirect ...

journal_title：Applied biochemistry and biotechnology

authors： Richardson PJ,Luzio JP

更新日期：1986-10-01 00:00:00

abstract：:We have described a novel red biochrome, 514 Da in size, produced by solid-state cultivation of a bacterial isolate obtained from garden soil. The growth requirements of the isolate, the chemical characteristics of the biochrome produced, and the application of the biochrome in dying of silk, wool, and cotton fabrics ...

journal_title：Applied biochemistry and biotechnology

authors： Vaidyanathan J,Bhathena-Langdana Z,Adivarekar RV,Nerurkar M

更新日期：2012-01-01 00:00:00

abstract：:Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techni...

journal_title：Applied biochemistry and biotechnology

authors： Ximenes EA,Brandon SK,Doran-Peterson J

更新日期：2008-03-01 00:00:00

abstract：:The transesterification of soybean lecithin with ethyl esters of polyunsaturated fatty acids (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) using immobilized lipase from Rhizomucor miehei was tested in the presence or absence of organic solvent (hexane) and additives (urea with Ca(2+) or Mg(2+)). The rea...

journal_title：Applied biochemistry and biotechnology

authors： Marsaoui N,Naghmouchi K,Baah J,Raies A,Laplante S

更新日期：2015-06-01 00:00:00

abstract：:The objective of this study was to understand the effect of pH and ionic strength of aluminum sulfate on the flocculation of microalgae. It was found that changing pH and ionic strength influenced algal flocculation by changing the zeta potential of cells, which was described by the classical theory of Derjaguin, Land...

journal_title：Applied biochemistry and biotechnology

authors： Cui Y,Yuan W,Cheng J

更新日期：2014-08-01 00:00:00

abstract：:Lignocellulosic biomass has great potential as a cheap feedstock in biological processes to produce biofuels or chemicals; however, dilute acid pretreatment at high temperatures produces undesirable compounds. Toxicity tests were done with inhibitors in standard media, to predict the growth-limiting effects on thermop...

journal_title：Applied biochemistry and biotechnology

authors： Thomasser C,Danner H,Neureiter M,Saidi B,Braun R

更新日期：2002-04-01 00:00:00

abstract：:Acrylamide/chitosan-based cryogel was fabricated, and a triazine dye, Reactive Green 5, was attached to the cryogel by nucleophilic substitution to build a dye affinity support for adsorption of catalase enzyme. Characterization of cryogel was performed using FTIR, SEM, EDX, BET, and swelling test. Synthesized cryogel...

journal_title：Applied biochemistry and biotechnology

authors： Yavaşer R,Karagözler AA

更新日期：2020-12-01 00:00:00

abstract：:In this presented work, magnetic poly(HEMA-GMA) nanoparticles were synthesized, characterized, and used for immobilization of an anti-leukemic enzyme L-asparaginase. The average particle size of the synthesized magnetic nanoparticles was found to be as 117.5 nm. L-asparaginase was successfully immobilized onto the syn...

journal_title：Applied biochemistry and biotechnology

authors： Orhan H,Aktaş Uygun D

更新日期：2020-08-01 00:00:00

abstract：:Large-scale amine-based CO(2) capture will generate waste containing large amounts of ammonia, in addition to contaminants such as the actual amine as well as degradation products thereof. Monoethanolamine (MEA) has been a dominant amine applied so far in this context. This study reveals how biological N removal can b...

journal_title：Applied biochemistry and biotechnology

authors： Hauser I,Colaço AB,Skjæran JA,Einbu A,Ostgaard K,Svendsen HF,Cervantes FJ

更新日期：2013-02-01 00:00:00

abstract：:The kinetic parameters of the oxidant action of the combination of enzyme horseradish peroxidase (HRP) with hydrogen peroxide in the degradation of methylene blue dye were investigated. Twenty-one percent of color removal was obtained at pH 5.0 and temperature of 30 °C. Under these conditions, the kinetic parameters K...

journal_title：Applied biochemistry and biotechnology

authors： Pereira AR,da Costa RS,Yokoyama L,Alhadeff EM,Teixeira LA

更新日期：2014-12-01 00:00:00

abstract：:Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Ps...

journal_title：Applied biochemistry and biotechnology

authors： Lee SA,Wrona LJ,Cahoon AB,Crigler J,Eiteman MA,Altman E

更新日期：2016-01-01 00:00:00

abstract：:Biosurfactants are of considerable interest due to their biodegradability, low degree of toxicity, and diverse applications. However, the high production costs involved in the acquisition of biosurfactants underscore the need for optimization of the production process to enable viable application on an industrial scal...

journal_title：Applied biochemistry and biotechnology

authors： Campos JM,Stamford TL,Sarubbo LA

更新日期：2014-03-01 00:00:00

abstract：:The aim of the present study was bioremediation of distillery sludge into a soil-enriching material. It was mixed with a complementary waste, cattle dung, and subjected to vermicomposting with (V) and without (T, control) Eisenia fetida in the ratio of 0:100 % (V1, T1), 10:90 (V2, T2), 25:75 (V3, T3), 50:50 (V4, T4), ...

journal_title：Applied biochemistry and biotechnology

authors： Singh J,Kaur A,Vig AP

更新日期：2014-10-01 00:00:00

abstract：:In previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the YR-1 strain of Mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sAO) activity. In the present work enzym...

journal_title：Applied biochemistry and biotechnology

authors： Silva-Jiménez H,Zazueta-Sandoval R

更新日期：2005-04-01 00:00:00

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:This article deals with a direct electrochemical method of detecting antigens using new methods of functionalization of gold electrodes. Based on the reacting ability of gold with sulfhydryl groups, three protocols for the fixation of antibodies have been explored. They are based on either the self-assembling properti...

journal_title：Applied biochemistry and biotechnology

authors： Ameur S,Martelet C,Jaffrezic-Renault N,Chovelon JM

更新日期：2000-11-01 00:00:00