Gelatin-immobilised poly(hydroxyethyl methacrylate) cryogel for affinity purification of fibronectin.

abstract：:Silver nanoparticles (AgNPs) were synthesised using Kalopanax septemlobus plant leaf extracts. UV-visible spectrophotometric, Fourier-transform infrared, electron dispersive X-ray spectroscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analyses confirmed synthesis of AgNPs. TEM micr...

journal_title：Applied biochemistry and biotechnology

authors： Salunke BK,Sawant SS,Kim BS

更新日期：2014-09-01 00:00:00

abstract：:The enzyme 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGR) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. This enzyme has three domains: transmembrane, binding, and soluble. In this study, we expressed and puri...

journal_title：Applied biochemistry and biotechnology

authors： Andrade-Pavón D,Cuevas-Hernández RI,Trujillo-Ferrara JG,Hernández-Rodríguez C,Ibarra JA,Villa-Tanaca L

更新日期：2017-08-01 00:00:00

abstract：:Two types of (CNBr-activated) Agarose-staphylococcal endonuclease derivatives have been prepared, one with the enzyme uniformly distributed in the support, and the other with the enzyme preferentially bound in the most external part of the support particles; the latter were obtained using agarose of very small pores a...

journal_title：Applied biochemistry and biotechnology

authors： Guisan JM,Serrano J,Melo FV,Ballesteros A

更新日期：1987-02-01 00:00:00

abstract：:Paraoxonase 1 (PON1: EC 3.1.8.1) is a calcium-dependent enzyme associated with high-density lipoproteins (HDLs) and has a protective effect against oxidation of low-density lipoproteins (LDLs) in mammals. PON1 is the best-studied member of a family of enzymes called serum paraoxonases, or PONs, identified in mammals a...

journal_title：Applied biochemistry and biotechnology

authors： Erzengin M,Demir D,Arslan M,Sinan S

更新日期：2014-08-01 00:00:00

abstract：:Indole-3-carbinol (I3C) is a naturally occurring glucosinolate found in Brassica vegetables that is usually converted in gastric acidic environment to the efficient metabolite 3,3'-diindolylmethane (DIM). Both indoles (I3C and DIM) are known chemopreventive agents for various cancers including breast cancer. This stud...

journal_title：Applied biochemistry and biotechnology

authors： El-Daly SM,Gamal-Eldeen AM,Gouhar SA,Abo-Elfadl MT,El-Saeed G

更新日期：2020-12-01 00:00:00

abstract：:Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growt...

journal_title：Applied biochemistry and biotechnology

authors： Cai Z,Riedel H,Thaw Saw NM,Kütük O,Mewis I,Jäger H,Knorr D,Smetanska I

更新日期：2011-06-01 00:00:00

abstract：:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was a...

journal_title：Applied biochemistry and biotechnology

authors： Lianghua T,Liming X,Min S,Huaying G

更新日期：2007-08-01 00:00:00

abstract：:A comparative study was carried out to evaluate protease production in solid-state fermentation (SSF) and submerged fermentation (SmF) by nine different thermophilic fungi--Thermoascus aurantiacus Miehe, Thermomyces lanuginosus, T. lanuginosus TO.03, Aspergillus flavus 1.2, Aspergillus sp. 13.33, Aspergillus sp. 13.34...

journal_title：Applied biochemistry and biotechnology

authors： Macchione MM,Merheb CW,Gomes E,da Silva R

更新日期：2008-03-01 00:00:00

abstract：:The effect of biosurfactant rhamnolipid (RL) on hydrolysis and acidification of waste activated sludge (WAS) was investigated. The results indicated that RL could greatly reduce the surface tension of sludge, resulting in stimulating the hydrolysis rate of WAS and enhancing the production of short-chain fatty acids (S...

journal_title：Applied biochemistry and biotechnology

authors： Yi X,Luo K,Yang Q,Li XM,Deng WG,Cheng HB,Wang ZL,Zeng GM

更新日期：2013-11-01 00:00:00

abstract：:Lipases are an enzyme class of a great importance as biocatalysts applied to organic chemistry. However, it is still necessary to search for new enzymes with special characteristics such as good stability towards high temperatures, organic solvents, and high stereoselectivity presence. The present work's aim was to im...

journal_title：Applied biochemistry and biotechnology

authors： Cunha AG,Fernández-Lorente G,Gutarra ML,Bevilaqua JV,Almeida RV,Paiva LM,Fernández-Lafuente R,Guisán JM,Freire DM

更新日期：2009-05-01 00:00:00

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:The decrease in toxicity of carbon capture reclaimer monoethanolamine (MEA) waste (MEAw) during anaerobic degradation of such waste together with easily degradable organics was investigated. Samples were collected from a bioreactor at steady state with 86 % organic chemical oxygen demand removal at room temperature, w...

journal_title：Applied biochemistry and biotechnology

authors： Wang S,Hovland J,Brooks S,Bakke R

更新日期：2014-01-01 00:00:00

abstract：:A new medical application of an immobilized microbial enzyme is described. Extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious hemorrhagic complications. Heparinase isolated from Flavobacterium has been immobilized and used in...

journal_title：Applied biochemistry and biotechnology

authors： Linhardt RJ,Cooney CL,Tapper D,Zannetos CA,Larsen AK,Langer R

更新日期：1984-02-01 00:00:00

abstract：:The metabolism of residual glycerol from biodiesel synthesis by Klebsiella pneumoniae BLh-1 was investigated in this study. Batch and fed-batch cultivations were performed in bioreactors under anaerobic and oxygen limitation conditions. Results of batch cultivations showed that the main product was 1,3-propanediol (1,...

journal_title：Applied biochemistry and biotechnology

authors： Rossi DM,de Souza EA,Ayub MA

更新日期：2013-03-01 00:00:00

abstract：:When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of de...

journal_title：Applied biochemistry and biotechnology

authors： Tabbene O,Karkouch I,Slimene IB,Elfeddy N,Cosette P,Mangoni ML,Jouenne T,Limam F

更新日期：2011-05-01 00:00:00

abstract：:The thermostable esterase from the thermophilic bacterium Clostridium thermocellum DSM 1313 was expressed in Escherichia coli and purified by Ni(2+) affinity chromatography. Its molecular weight was approximately 35 kDa according to 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Th...

journal_title：Applied biochemistry and biotechnology

authors： Zhang T,Chen H,Ni Z,Tian R,Jia J,Chen Z,Yang S

更新日期：2015-12-01 00:00:00

abstract：:This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.12 day-1 was obta...

journal_title：Applied biochemistry and biotechnology

authors： Sun ZL,Sun LQ,Chen GZ

更新日期：2019-04-01 00:00:00

abstract：:A total of 49 protein sequences of alkaline proteases retrieved from GenBank representing different species of Aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. The sequence level h...

journal_title：Applied biochemistry and biotechnology

authors： Morya VK,Yadav S,Kim EK,Yadav D

更新日期：2012-01-01 00:00:00

abstract：:The pLKO.1-TRC plasmid has been a popular and widely used vector due to its simple handling and stability. The huge RNAi database, a TRC library, has been established based on this vector. However, this plasmid only has a puromycin-resisted gene for selecting, which limits its application in microscopy and fluorescenc...

journal_title：Applied biochemistry and biotechnology

authors： Xu J,Zhu W,Guo Y,Jiang C,Hussain N,Meng L,Lu S

更新日期：2017-02-01 00:00:00

abstract：:A study was undertaken to examine the influence of biogenic nanoparticles synthesized from Tridax procumbens on different parameters of seed germination, seedling growth, and various biochemical parameters in four Eruca sativa varieties having low percentage of germination. Seeds were treated with different concentrat...

journal_title：Applied biochemistry and biotechnology

authors： Ushahra J,Bhati-Kushwaha H,Malik CP

更新日期：2014-09-01 00:00:00

abstract：:Prevalence of heavy metals in the living environment causes chemical stress and reactive oxygen species (ROS) formation in Phanerochaete chrysosporium (P. chrysosporium). However, the mechanisms involved in ROS defense are still under investigation. In the present study, we evaluated the effect of lead- and cadmium-in...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Q,Zeng G,Chen G,Yan M,Chen A,Du J,Huang J,Yi B,Zhou Y,He X,He Y

更新日期：2015-02-01 00:00:00

abstract：:Tradescantia pallida (Wandering jew)-a succulent perennial herb-was screened to be a potent chromium (Cr) accumulator. Its ability to grow under Cr stress was examined by studying biochemical changes and physiological response of the plant in presence of 5-20 mg L(-1) Cr(VI) concentration in hydroponic environment for...

journal_title：Applied biochemistry and biotechnology

authors： Sinha V,Pakshirajan K,Chaturvedi R

更新日期：2014-08-01 00:00:00

abstract：:Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subti...

journal_title：Applied biochemistry and biotechnology

authors： Kodrík D,Kludkiewicz B,Navrátil O,Skoková Habuštová O,Horáčková V,Svobodová Z,Vinokurov KS,Sehnal F

更新日期：2013-09-01 00:00:00

abstract：:A DNA fragment of 2,042 bp containing a novel beta-mannanase gene, man5A, was identified from the genome of the mannan-degrading bacterium Bacillus circulans CGMCC1554. The open reading frame of man5A comprised 978 bp encoding a protein of 326 amino acids with a predicted molecular weight of 32 kDa. The amino acid seq...

journal_title：Applied biochemistry and biotechnology

authors： Yang P,Li Y,Wang Y,Meng K,Luo H,Yuan T,Bai Y,Zhan Z,Yao B

更新日期：2009-10-01 00:00:00

abstract：:Growth kinetics and red pigment production of Monascus purpureus CCT 3802 was studied. A reproducible inoculum with extremely dispersed hyphae for bioreactor runs was obtained through a two-step cultivation in a shaker. First, the spores were cultivated in a complex medium rendering a suspension of vegetative cells. I...

journal_title：Applied biochemistry and biotechnology

authors： Pereira DG,Kilikian BV

更新日期：2001-04-01 00:00:00

abstract：:Environmental conditions affect the production of extracellular polysaccharide by Azotobacter chroococcum ATCC 4412. Production of exocellular polymer from a variety of carbon sources depended on the air flow rate. A high sucrose concentration in medium (8%) markedly favored expopolysaccharide production, which reache...

journal_title：Applied biochemistry and biotechnology

authors： De la Vega MG,Cejudo FJ,Paneque A

更新日期：1991-09-01 00:00:00

abstract：:A genetically engineered Escherichia coli cell expressing both organophosphorus hydrolase (OPH) and carboxyl esterase (CaE) B1 intracellularly was constructed and cultivated. The harvested wet cells were vacuum dried, and the storage stability of the dried cell powder was determined in terms of OPH activity. Over a pe...

journal_title：Applied biochemistry and biotechnology

authors： Zheng YZ,Lan WS,Qiao CL,Mulchandani A,Chen W

更新日期：2007-03-01 00:00:00

abstract：:In this study, the feasibility of introducing redox property to an amphiphilic phospholipid polymer (PMBN) was investigated. The active ester group in the side chain of the polymer was used to react with pyrroloquinoline quinine (PQQ). Redox peaks that corresponded to PQQ redox potentials were observed after the modif...

journal_title：Applied biochemistry and biotechnology

authors： Yu EH,Himuro Y,Takai M,Ishihara K

更新日期：2010-02-01 00:00:00

abstract：:Nostoc microscopicum an effective extracellular polymers (EPs) synthesizer among cyanobacteria was isolated, and its elementary morphological features were defined with the aid of light microscope and CLSM. Bioseparation of EPs from 44 days-aged culture gave 0.90 g/L of the dry powdered extract. Chemical quantificatio...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran L,Marappa N,Sethumadhavan K,Nooruddin T

更新日期：2020-05-01 00:00:00

abstract：:NADP(+)-dependent isocitrate dehydrogenase from Yarrowia lipolytica CLIB122 (YlIDP) was overexpressed and purified. The molecular mass of YlIDP was estimated to be about 81.3 kDa, suggesting its homodimeric structure in solution. YlIDP was divalent cation dependent and Mg(2+) was found to be the most favorable cofacto...

journal_title：Applied biochemistry and biotechnology

authors： Li X,Wang P,Ge Y,Wang W,Abbas A,Zhu G

更新日期：2013-09-01 00:00:00