Expression and characterization of a new thermostable esterase from Clostridium thermocellum.

Abstract:

:The thermostable esterase from the thermophilic bacterium Clostridium thermocellum DSM 1313 was expressed in Escherichia coli and purified by Ni(2+) affinity chromatography. Its molecular weight was approximately 35 kDa according to 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. The enzyme exhibited the highest specific activity with p-nitrophenyl butyrate (285 s(-1) mM(-1)). The activity of the esterase was greatest at 65 °C, and the esterase maintained residual activity levels of 70 and 50 % after 3 h incubation at 65 and 70 °C, respectively. Its activity was optimal at pH 7.0, was enhanced in the presence of Ca(2+) and Mg(2+), and was inhibited by Ni(2+) and Cu(2+). The addition of surfactants, such as Tween-20, Tween-80, Triton X-100, and SDS, at concentrations of 5 % (v/v) significantly inhibited the lipolytic action of the esterase. Enzyme activity was relatively stable in 10 % methanol, and 50 % residual activity was seen in 10 % DMSO, demonstrating its potential in biodiesel production and industrial applications.

authors

Zhang T,Chen H,Ni Z,Tian R,Jia J,Chen Z,Yang S

doi

10.1007/s12010-015-1824-7

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

1437-46

issue

7

eissn

0273-2289

issn

1559-0291

pii

10.1007/s12010-015-1824-7

journal_volume

177

pub_type

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