Organic and Inorganic Nitrogen Impact Chlorella variabilis Productivity and Host Quality for Viral Production and Cell Lysis.

abstract：:We report the identification of five proteins expressed specifically on the bacterial magnetic particle (BMP) membrane of Magnetospirillum sp. AMB-1. These proteins are major components of the BMP membrane. The molecular weights were determined to be 12.0, 16.0, 24.8, 35.6, and 66.2 kDa by sodium dodecyl sulfate polya...

journal_title：Applied biochemistry and biotechnology

authors： Okamura Y,Takeyama H,Matsunaga T

更新日期：2000-04-01 00:00:00

abstract：:UDPG pyrophosphatase (UGPase) plays an important role in carbohydrate metabolism, catalyzing a reversible production of uridine diphosphate glucose (UDPG) and pyrophosphate (PPi) from Glc-1-P and UTP. UGPase gene from Aureobasidium pullulans NRRL Y-12974 was cloned, overexpressed in Escherichia coli. The recombinant U...

journal_title：Applied biochemistry and biotechnology

authors： Li H,Zhang Y,Gao Y,Lan Y,Yin X,Huang L

更新日期：2016-03-01 00:00:00

abstract：:RNA interference (RNAi) is an evolutionary conserved gene regulation pathway that has emerged as an important discovery in the field of molecular biology. One of the important advantages of RNAi in therapy is that it brings about efficient downregulation of gene expression by targeting complementary transcripts in com...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran PV,Ignacimuthu S

更新日期：2013-03-01 00:00:00

abstract：:Biological control of soil-borne plant pathogens is a potential alternative to the use of chemical pesticides, which have already been proved to be harmful to the environment. Several strains of the fungus Trichoderma have been isolated and found to be effective biocontrol agents of various soil-borne plant pathogenic...

journal_title：Applied biochemistry and biotechnology

authors： Chet I,Inbar J

更新日期：1994-07-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00

abstract：:Serratia marcescens SP511 was previously reported to be an L-proline-producing strain that harbors a recombinant plasmid carrying the mutant type of the proline operon. This strain produced 65 g/L of L-proline in a medium containing 22% sucrose and urea after 5 d of incubation under the conventional culture conditions...

journal_title：Applied biochemistry and biotechnology

authors： Masuda M,Takamatu S,Nishimura N,Komatsubara S,Tosa T

更新日期：1993-12-01 00:00:00

abstract：:In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high eth...

journal_title：Applied biochemistry and biotechnology

authors： Ding J,Gao M,Hou G,Liang K

更新日期：2015-08-01 00:00:00

abstract：:The epithelial-mesenchymal transition (EMT) is a process in which epithelial cells lose their cell polarity and cell-cell adhesion, and gain migratory and invasive properties. It is believed that EMT is associated with initiation and completion of the invasion-metastasis cascade. In this study, an economic approach wa...

journal_title：Applied biochemistry and biotechnology

authors： Li Y,Gao A,Yu L

更新日期：2016-01-01 00:00:00

abstract：:An extracellular thermostable xylanase from a newly isolated thermophilic Actinomadura sp. strain Cpt20 was purified and characterized. Based on matrix-assisted laser desorption-ionization time-of-flight mass spectrometry analysis, the purified enzyme is a monomer with a molecular mass of 20,110.13 Da. The 19 residue ...

journal_title：Applied biochemistry and biotechnology

authors： Taibi Z,Saoudi B,Boudelaa M,Trigui H,Belghith H,Gargouri A,Ladjama A

更新日期：2012-02-01 00:00:00

abstract：:The enzymatic alcoholysis of soybean oil with methanol and ethanol was investigated using a commercial, immobilized lipase (Lipozyme RMIM). The effect of alcohol (methanol or ethanol), enzyme concentration, molar ratio of alcohol to soybean oil, solvent, and temperature on biodiesel production was determined. The best...

journal_title：Applied biochemistry and biotechnology

authors： Bernardes OL,Bevilaqua JV,Leal MC,Freire DM,Langone MA

更新日期：2007-04-01 00:00:00

abstract：:Proinflammatory cytokines such as tumor necrosis factor (TNF) alpha are well known to inhibit adipocyte differentiation. TNF-alpha triggers ceramide synthesis through binding of TNF-alpha to its p55 receptor. Therefore, ceramide is implicated in many of the multiple signaling pathways initiated by TNF-alpha. In breast...

journal_title：Applied biochemistry and biotechnology

authors： Xu F,Yang CC,Gomillion C,Burg KJ

更新日期：2010-01-01 00:00:00

abstract：:A bench scale experimental system was developed for the analysis of polycyclic aromatic hydrocarbon (PAH) degradation by mixed microbial cultures in PAH contaminated Manufactured Gas Plant (MGP) soils and on sand. The reactor system was chosen in order to provide a fundamental protocol capable for evaluating the perfo...

journal_title：Applied biochemistry and biotechnology

authors： Webb OF,Phelps TJ,Bienkowski PR,DiGrazia PM,Reed GD,Applegate B,White DC,Sayler GS

更新日期：1991-04-01 00:00:00

abstract：:A lactate oxidase was purified about 36-fold from a newly screened strain KY6 of gram negative bacterium from soil to yield a homogeneous protein. The native enzyme had a molecular mass of 204 kDa measured by Sephadex G-200 and that of subunit on the SDS-PAGE was found to be 45 kDa. The enzyme was optimally active at ...

journal_title：Applied biochemistry and biotechnology

authors： Xu P,Yano T,Yamamoto K,Suzuki H,Kumagai H

更新日期：1996-03-01 00:00:00

abstract：:Aspergillus niger phytase (PhyA) has been used as a feed supplement to improve the bioavailability of phytate phosphorus to swine and poultry. However, it is unable to maintain its stability due to high temperature during the feed pelleting process. In this study, we performed site-directed mutagenesis in the Aspergil...

journal_title：Applied biochemistry and biotechnology

authors： Liao Y,Li CM,Chen H,Wu Q,Shan Z,Han XY

更新日期：2013-10-01 00:00:00

abstract：:Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of...

journal_title：Applied biochemistry and biotechnology

authors： Xia C,Wei W,Hu B

更新日期：2014-04-01 00:00:00

abstract：:A poly-gamma-glutamic acid (gamma-PGA) productive strain, halotolerant bacterium WX-02 was isolated from the saline soil of China (Yingcheng). By physiological, biochemical, and 16S rDNA sequence analysis methods, the strain was identified as Bacillus licheniformis. The effect of NaCl concentration on gamma-PGA produc...

journal_title：Applied biochemistry and biotechnology

authors： Wei X,Ji Z,Chen S

更新日期：2010-03-01 00:00:00

abstract：:The Pichia stipitis xylose reductase gene (XYL1) was inserted into an autonomous plasmid that P. stipitis maintains in multicopy. The plasmid pXOR with the XYL1 insert or a control plasmid pJM6 without XYL1 was introduced into P. stipitis. When grown on xylose under aerobic conditions, the strain with pXOR had up to 1...

journal_title：Applied biochemistry and biotechnology

authors： Dahn KM,Davis BP,Pittman PE,Kenealy WR,Jeffries TW

更新日期：1996-04-01 00:00:00

abstract：:Internal air circulation affects the temperature field distribution in a gas double-dynamic solid-state fermentation bioreactor (GDSFB). To enhance heat transfer through strengthening internal air circulation in a GDSFB, we put an air distribution plate (ADP) into the bioreactor and studied the effects of forced inter...

journal_title：Applied biochemistry and biotechnology

authors： Chen H,Qin L,Li H

更新日期：2014-02-01 00:00:00

abstract：:The saccharification of marine microalgae using amylase from marine bacteria in saline conditions was investigated. An amylase-producing bacterium, Pseudoalterimonas undina NKMB 0074 was isolated and identified. The green microalga NKG 120701 was determined to have the highest concentration of intracellular carbohydra...

journal_title：Applied biochemistry and biotechnology

authors： Matsumoto M,Yokouchi H,Suzuki N,Ohata H,Matsunaga T

更新日期：2003-04-01 00:00:00

abstract：:Although the use of lipases as biocatalysts has frequently been proposed, it is yet scarcely being implemented in industrial processes. This is mainly due to the difficulties associated with the discovery and engineering of new enzymes and the lack of versatile screening methods. In this study, we screened the availab...

journal_title：Applied biochemistry and biotechnology

authors： Roh C,Schmid RD

更新日期：2013-12-01 00:00:00

abstract：:The native Celluclast BG cellulase enzyme complex consists of different enzymes which can also degrade great substrate molecules as native celluloses. This enzyme complex has been covered by a very thin, a few nanometers thick, polymer layer, in order to improve its stability. It has been proved that the polymer layer...

journal_title：Applied biochemistry and biotechnology

authors： Hegedüs I,Hancsók J,Nagy E

更新日期：2012-11-01 00:00:00

abstract：:The partition behavior of proteins, nucleic acids, cell membranes, cell organelles and whole cells has been studied in liquid-liquid two-phase systems composed of water, poly-(ethylene glycol), and an hydroxypropyl starch. The properties of the systems are in many respects comparable with the traditional poly(ethylene...

journal_title：Applied biochemistry and biotechnology

authors： Sturesson S,Tjerneld F,Johansson G

更新日期：1990-12-01 00:00:00

abstract：:Vegetable and fruit waste (VFW) is becoming a heavy burden of municipal waste disposal because of its huge amount, but it is a potentially valuable resource that can be developed into high value products such as methane. Conventional anaerobic digestion processes are not suitable for solving the problem of easy acidif...

journal_title：Applied biochemistry and biotechnology

authors： Liu WY,Liao B

更新日期：2019-05-01 00:00:00

abstract：:The feasibility of using mixtures of C4 and C5 chain-length aliphatic alcohols from fusel oil as the bulk organic media for lipase-mediated synthesis of laurate esters was assessed. Reaction mixtures consisted of lauric acid, lipase, solvent (if added), and appropriate amount of fusel oil (previously dehydrated with i...

journal_title：Applied biochemistry and biotechnology

authors： De Castro HF,Moriya RY,Oliveira PC,Soares CM

更新日期：1999-04-01 00:00:00

abstract：:This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.12 day-1 was obta...

journal_title：Applied biochemistry and biotechnology

authors： Sun ZL,Sun LQ,Chen GZ

更新日期：2019-04-01 00:00:00

abstract：:Large-scale amine-based CO(2) capture will generate waste containing large amounts of ammonia, in addition to contaminants such as the actual amine as well as degradation products thereof. Monoethanolamine (MEA) has been a dominant amine applied so far in this context. This study reveals how biological N removal can b...

journal_title：Applied biochemistry and biotechnology

authors： Hauser I,Colaço AB,Skjæran JA,Einbu A,Ostgaard K,Svendsen HF,Cervantes FJ

更新日期：2013-02-01 00:00:00

abstract：:The currently used multistep chemical synthesis for making surfaces antimicrobial by attaching to them hydrophobic polycations is replaced herein by an aerosol-assisted plasma deposition procedure. To this end, N,N-hexyl,methyl-PEI (HMPEI) is directly plasma-coated onto a glass surface. The resultant immobilized HMPEI...

journal_title：Applied biochemistry and biotechnology

authors： Liu H,Kim Y,Mello K,Lovaasen J,Shah A,Rice N,Yim JH,Pappas D,Klibanov AM

更新日期：2014-02-01 00:00:00

abstract：:The enzyme cellobiase from Novo was immobilized in controlled pore silica particles by covalent binding with the silane-glutaraldehyde method with protein and activity yields of 67 and 13.7%, respectively. The activity of the free enzyme (FE) and immobilized enzyme (IE) was determined with 2 g/L of cellobiose, from 40...

journal_title：Applied biochemistry and biotechnology

authors： Calsavara LP,De Moraes FF,Zanin GM

更新日期：2001-04-01 00:00:00

abstract：:The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, ...

journal_title：Applied biochemistry and biotechnology

authors： Lee S,Lee S,Yoon YJ,Lee J

更新日期：2013-01-01 00:00:00

abstract：:Fructose-1,6-bisphosphate (F-1,6-P2) is an allosteric activator of two key enzymes of glycolysis: phosphofructokinase and pyruvate kinase. Regulation of glycolysis in a wild-type Saccharomyces cerevisiae and a recombinant Escherichia coli by a dead-end structural analog of F-1,6-P2 was studied. 2,5-Anhydromannitol (2,...

journal_title：Applied biochemistry and biotechnology

authors： Nghiem NP,Cofer TM

更新日期：2007-05-01 00:00:00