Isolation of an organic solvent-tolerant lipolytic enzyme from uncultivated microorganism.

abstract：:Phytases act on phytic acid, an antinutrient factor present in animal feeds, and release inorganic phosphate. We optimized the production parameters for phytase production using Thermoascus aurantiacus (TUB F 43), a thermophilic fungal culture, by submerged fermentation. A semisynthetic medium containing glucose, star...

journal_title：Applied biochemistry and biotechnology

authors： Nampoothiri KM,Tomes GJ,Roopesh K,Szakacs G,Nagy V,Soccol CR,Pandey A

更新日期：2004-07-01 00:00:00

abstract：:A new medical application of an immobilized microbial enzyme is described. Extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious hemorrhagic complications. Heparinase isolated from Flavobacterium has been immobilized and used in...

journal_title：Applied biochemistry and biotechnology

authors： Linhardt RJ,Cooney CL,Tapper D,Zannetos CA,Larsen AK,Langer R

更新日期：1984-02-01 00:00:00

abstract：:Outdoor production of microalgae could be set on a sound industrial basis if solar energy were utilized at a much higher efficiency than presently obtained. Many types of photobioreactors have been developed in the past in an attempt to answer this challenge, but their photosynthetic efficiency has been rather similar...

journal_title：Applied biochemistry and biotechnology

authors： Richmond A,Qiang H

更新日期：1997-04-01 00:00:00

abstract：:This study shows that electroanalysis may be used in vanillin biotechnological production. As a matter of fact, vanillin and some molecules implicated in the process like eugenol, ferulic acid, and vanillic acid may be oxidized on electrodes made of different materials (gold, platinum, glassy carbon). By a judicious c...

journal_title：Applied biochemistry and biotechnology

authors： Giraud W,Mirabel M,Comtat M

更新日期：2014-02-01 00:00:00

abstract：:To screen strains of halotolerant or halophile bacteria which are able to convert isoeugenol to vanillin, 36 different strains of bacteria isolated from the salty environments in Iran were investigated. During growth on isoeugenol, a moderately halotolerant Gram-negative coccobacil showed capability of converting isoe...

journal_title：Applied biochemistry and biotechnology

authors： Ashengroph M,Nahvi I,Zarkesh-Esfahani H,Momenbeik F

更新日期：2012-01-01 00:00:00

abstract：:The common view of amyloids and prion proteins is that they are associated with many currently incurable diseases and present a great danger to an organism. This danger comes from the fact that not only prion proteins, but also the infectious form(s) of amyloids, as it has been shown recently, are able to transmit the...

journal_title：Applied biochemistry and biotechnology

authors： Pulawski W,Ghoshdastider U,Andrisano V,Filipek S

更新日期：2012-04-01 00:00:00

abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...

journal_title：Applied biochemistry and biotechnology

authors： Filiz E,Koc I,Ozyigit II

更新日期：2014-07-01 00:00:00

abstract：:The use of cheap, high-efficiency, and ecofriendly adsorbent has been studied as an alternative way for the removal of dyes from wastewater. This paper investigated the use of waste brewery's yeast for the removal of acid orange 7 from aqueous solution. The optimum removal of acid orange 7 was found to be 3.561 mg/g a...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Hu Y,Xie Z,Feng S,Li B,Mi X

更新日期：2011-04-01 00:00:00

abstract：:In order to detect low levels of enzyme activity, specifically glucose oxidase, in biological samples, an immunoenzymatic assay was developed since currently available methods could not be used because of either their lack of sensitivity or the conditions prevailing in our samples: turbidity of the medium, presence of...

journal_title：Applied biochemistry and biotechnology

authors： de Bellefontaine C,Josse F,Domurado M,Domurado D

更新日期：1994-08-01 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of immobilizing Yarrowia lipolytica lipase lip2 on epoxy microspheres with or without gelatin modifications. The activity of lipase immobilized on gelatin-modified supports was twofold higher than those immobilized on native supports. There was no significan...

journal_title：Applied biochemistry and biotechnology

authors： Xie R,Cui C,Chen B,Tan T

更新日期：2015-10-01 00:00:00

abstract：:Feather waste, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Feather-degrading bacteria were isolated from a Brazilian poultry industrial waste. Among these isolates, a strain identified as kr2 was th...

journal_title：Applied biochemistry and biotechnology

authors： Sangali S,Brandelli A

更新日期：2000-04-01 00:00:00

abstract：:In rapidly growing developing countries, waste disposal is a major challenge. Current waste disposal methods (e.g., landfills and sewage treatment) incur costs and often are not employed; thus, wastes accumulate in the environment. To address this challenge, it is advantageous to create economic incentives to collect ...

journal_title：Applied biochemistry and biotechnology

authors： Lonkar S,Fu Z,Wales M,Holtzapple M

更新日期：2017-01-01 00:00:00

abstract：:γ-Aminobutyric acid (γ-GABA) is a non-proteinogenic amino acid, which acts as a major regulator in the central nervous system. Glutamate decarboxylase (namely GAD, EC 4.1.1.15) is known to be an ideal enzyme for γ-GABA production using L-glutamic acid as substrate. In this study, we cloned and expressed GAD gene from ...

journal_title：Applied biochemistry and biotechnology

authors： Xiong Q,Xu Z,Xu L,Yao Z,Li S,Xu H

更新日期：2017-12-01 00:00:00

abstract：:The amylase from Aureobasidium pullulans Cau 19 was purified by ammonium sulfate precipitation and Sephadex G-100 chromatography with a 9.25-fold increase in specific activity as compared to crude enzyme. Km and turn over values of the enzyme were 6.25 mg/mL and 5.0 × 102/min, respectively. Effect of different metal i...

journal_title：Applied biochemistry and biotechnology

authors： Mulay YR,Deopurkar RL

更新日期：2018-02-01 00:00:00

abstract：:Lung cancer remains a highly prevalent disease and a leading cause of cancer-related deaths worldwide. Currently, exploring antitumor drugs derived from herbs used in traditional Chinese medicine is increasingly becoming an attractive area of research. Paclitaxel (PTX), a highly effective chemotherapeutic drug, is wid...

journal_title：Applied biochemistry and biotechnology

authors： Tan KT,Li S,Li YR,Cheng SL,Lin SH,Tung YT

更新日期：2019-04-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:To determine the influence of various culture conditions, transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were grown in nine cultures with four variable conditions (storage of inoculated broth at 4 degrees C prior to incubation, agitation speed, isopropyl-beta-D-thiogalac...

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Ishii M,Junior AP,de Oliveira Nascimento L,de Souza LC,Cholewa O

更新日期：2004-04-01 00:00:00

abstract：:The batch culture of a newly isolated strain of a green microalga, Chlorella sorokiniana, was carried out using a conical helical tubular photobioreactor. The isolate was capable of good growth at 40 degrees C under an airstream enriched with 10% CO2. The maximum photosynthetic productivity was 34.4 g of dry biomass/(...

journal_title：Applied biochemistry and biotechnology

authors： Morita M,Watanabe Y,Saiki H

更新日期：2000-06-01 00:00:00

abstract：:Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, co...

journal_title：Applied biochemistry and biotechnology

authors： Bandaru VV,Bandaru SR,Somalanka SR,Mendu DR,Imandi SB,Bejawada SR,Medicherla NR,Devarajan T,Karothi J,Chityala A

更新日期：2007-12-01 00:00:00

abstract：:To date, two types of glycerol dehydratases have been reported: coenzyme B12-dependent and coenzyme B12-independent glycerol dehydratases. The three-dimensional structure of the former is a dimer of αβγ heterotrimer, while that of the latter is a homodimer. Their mechanisms of reaction are typically enzymatic radical ...

journal_title：Applied biochemistry and biotechnology

authors： Liu JZ,Xu W,Chistoserdov A,Bajpai RK

更新日期：2016-07-01 00:00:00

abstract：:The first step involved in the degradation of phthalate isomers (phthalate, isophthalate and terephthalate) is the double hydroxylation by respective aromatic-ring hydroxylating dioxygenases. These are two component enzymes consisting of 'oxygenase' and 'reductase' components. Soil isolate Pseudomonas aeruginosa strai...

journal_title：Applied biochemistry and biotechnology

authors： Karandikar R,Badri A,Phale PS

更新日期：2015-09-01 00:00:00

abstract：:Vacuolar processing enzymes (VPEs) have received considerable attention recently, as they exhibit caspase-1-like cleavage activity and regulate the process of PCD. However, knowledge about their detailed characteristics and structures is relatively limited. In this study, a gamma vacuolar processing enzyme gene, MhVPE...

journal_title：Applied biochemistry and biotechnology

authors： Ran K,Yang H,Sun X,Li Q,Jiang Q,Zhang W,Shen W

更新日期：2014-05-01 00:00:00

abstract：:The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid an...

journal_title：Applied biochemistry and biotechnology

authors： Palonen H,Thomsen AB,Tenkanen M,Schmidt AS,Viikari L

更新日期：2004-04-01 00:00:00

abstract：:Culture fluorescence measurement technique has the potential for on-line characterization of metabolic status of fermentation processes. Many fluorophores present inside the living cells such as NADH + H+, tryptophan, pyridoxine, and riboflavin fluoresce at specific excitation and emission wavelength combinations. Sin...

journal_title：Applied biochemistry and biotechnology

authors： Khanna S,Srivastava AK

更新日期：2009-05-01 00:00:00

abstract：:In our previous study, chitosan-alginate microcapsules were developed to protect egg yolk immunoglobulin (IgY) from gastric inactivation. The present study was undertaken to determine the effect of chitosan concentration (0-0.8%; w/v) on various properties of the microcapsules in order to produce the optimum chitosan-...

journal_title：Applied biochemistry and biotechnology

authors： Li XY,Jin LJ,Lu YN,Zhen YH,Li SY,Wang LH,Xu YP

更新日期：2009-12-01 00:00:00

abstract：:Renal calculi disease or known as kidney stone disease is the most common urological disorder in both men and women, although it is more prevalent in men. The lifetime chance for an individual to develop renal calculi is approximately 10% whereas the risk of recurrence in a 10-year period is 74%. Therefore, a diagnost...

journal_title：Applied biochemistry and biotechnology

authors： Wai-Hoe L,Wing-Seng L,Ismail Z,Lay-Harn G

更新日期：2009-10-01 00:00:00

abstract：:Tradescantia pallida (Wandering jew)-a succulent perennial herb-was screened to be a potent chromium (Cr) accumulator. Its ability to grow under Cr stress was examined by studying biochemical changes and physiological response of the plant in presence of 5-20 mg L(-1) Cr(VI) concentration in hydroponic environment for...

journal_title：Applied biochemistry and biotechnology

authors： Sinha V,Pakshirajan K,Chaturvedi R

更新日期：2014-08-01 00:00:00

abstract：:Degradation of known odor precursors in sludge during anaerobic digestion was systematically studied and simulated using the Anaerobic Digestion Model Number 1 (ADM1). The degradation of various protein fractions (particulate, soluble, and bound), volatile fatty acids (VFAs), lipids, and amino acids of primary sludge ...

journal_title：Applied biochemistry and biotechnology

authors： Aldin S,Tu F,Nakhla G,Ray MB

更新日期：2011-08-01 00:00:00

abstract：:Using microalgae to capture CO2 from flue gas is an ideal way to reduce CO2 emission, but this is challenged by the high cost of carbon capture and transportation. To address this problem, a bicarbonate-based integrated carbon capture and algae production system (BICCAPS) has been proposed, in which bicarbonate is use...

journal_title：Applied biochemistry and biotechnology

authors： Chi Z,Elloy F,Xie Y,Hu Y,Chen S

更新日期：2014-01-01 00:00:00

abstract：:A highly sensitive, selective, reversible, and reusable glucose sensor is developed by using molecularly imprinted polymer-based artificial receptors onto interdigital transducer. Sensor receptors were synthesized through bulk imprinting technology by using styrene as monomer, ethylene glycol dimethacrylate (EGDMA) as...

journal_title：Applied biochemistry and biotechnology

authors： Asghar N,Mustafa G,Yasinzai M,Al-Soud YA,Lieberzeit PA,Latif U

更新日期：2019-12-01 00:00:00