Abstract:
:Microbial oil accumulation via oleaginous fungi has some potential benefits because filamentous fungi can form pellets during cell growth and these pellets are easier to harvest from the culture broth than individual cells. This research studied the effect of various culture conditions on the pelletized cell growth of Mucor circinelloides and its lipid accumulation. The results showed that cell pelletization was positively correlated to biomass accumulation; however, pellet size was negatively correlated to the oil content of the fungal biomass, possibly due to the mass transfer barriers generated by the pellet structure. How to control the size of the pellet is the key to the success of the pelletized microbial oil accumulation process.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Xia C,Wei W,Hu Bdoi
10.1007/s12010-014-0759-8subject
Has Abstractpub_date
2014-04-01 00:00:00pages
3502-12issue
7eissn
0273-2289issn
1559-0291journal_volume
172pub_type
杂志文章abstract::The tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. Different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning e...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-1432-y
更新日期:2015-03-01 00:00:00
abstract::Cancer is one of the killer diseases in humans and needs alternate curative measures despite recent improvement in modern treatment modalities. Oncolytic virotherapy seems to be a promising nonconventional way to treat cancers. Newcastle disease virus (NDV), a poultry virus, is nonpathogenic to human and domestic anim...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-012-9700-1
更新日期:2012-08-01 00:00:00
abstract::Culture fluorescence measurement technique has the potential for on-line characterization of metabolic status of fermentation processes. Many fluorophores present inside the living cells such as NADH + H+, tryptophan, pyridoxine, and riboflavin fluoresce at specific excitation and emission wavelength combinations. Sin...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-008-8395-9
更新日期:2009-05-01 00:00:00
abstract::The bacterium Enterobacter A47 has demonstrated the ability to synthesise distinct exopolysaccharides (EPS) as a function of the substrate used. The culture's performance was evaluated in experiments using either glucose or xylose, as single carbon sources, and compared with the substrate (glycerol) used in previous s...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0560-0
更新日期:2014-01-01 00:00:00
abstract::Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02919393
更新日期:1998-02-01 00:00:00
abstract::A total of 49 protein sequences of alkaline proteases retrieved from GenBank representing different species of Aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. The sequence level h...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9420-y
更新日期:2012-01-01 00:00:00
abstract::The enzyme mannose isomerase (EC 5.3.1.7) catalyzes the isomerization of D-mannose and D-fructose. The conversion of mannose to fructose is the first step in the principal pathway of mannose dissimilation by Pseudomonas cepacia. This enzyme is induced during growth on medium containing mannose to levels three- to four...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02920243
更新日期:1990-04-01 00:00:00
abstract::The epithelial-mesenchymal transition (EMT) is a process in which epithelial cells lose their cell polarity and cell-cell adhesion, and gain migratory and invasive properties. It is believed that EMT is associated with initiation and completion of the invasion-metastasis cascade. In this study, an economic approach wa...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1862-1
更新日期:2016-01-01 00:00:00
abstract::Cationic 1,2-dioleoyl trimethyl ammonium propane (DOTAP) and neutral 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) are anchored on cysteamine (cyst), mercaptopropionic acid (MPA) monolayer (thiol monolayers) modified on an individual gold transducer. DOTAP and DOPE are mixed with gold nanoparticle (AuNP) to for...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-0992-1
更新日期:2014-10-01 00:00:00
abstract::The gate surface of an ion-sensitive field effect transistor (ISFET) was modified with Langmuir-Blodgett (LB) film composed of fatty acid or crown ether amphiphiles to examine their potentiometric response to H+ and K+ ions. The results demonstrate the possible use of the lipid films for preparing ISFET ion sensors. ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/BF02918526
更新日期:1993-04-01 00:00:00
abstract::In this presented work, magnetic poly(HEMA-GMA) nanoparticles were synthesized, characterized, and used for immobilization of an anti-leukemic enzyme L-asparaginase. The average particle size of the synthesized magnetic nanoparticles was found to be as 117.5 nm. L-asparaginase was successfully immobilized onto the syn...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-020-03276-z
更新日期:2020-08-01 00:00:00
abstract::The production of cellulase-free endoxylanase by the thermophilic fungus Thermomyces lanuginosus was investigated in semisolid fermentation and liquid fermentation. Different process variables were investigated in semisolid fermentation, employing corncob as the carbon source. The best results were with the following ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:84-86:1-9:821
更新日期:2000-04-01 00:00:00
abstract::Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 106 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structu...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-017-2668-0
更新日期:2018-06-01 00:00:00
abstract::Mouse CXCL14/BRAK is a monocyte-selective chemokine which is expressed in almost all normal tissues. A flood of reports on its new functions of tumor suppression and fat metabolism modulation has left CXCL14 a potential therapeutic candidate for these diseases. Therefore, a simple accessible method is on demand for la...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-011-9218-y
更新日期:2011-08-01 00:00:00
abstract::The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible to detect L-lactate...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:96:1-3:239
更新日期:2001-10-01 00:00:00
abstract::Tobacco plants can be used for the production of proteins for pharmaceutical applications. One of the most difficult and expensive tasks associated with this technology is isolating the product of interest from the hundreds of other chemicals found in tobacco. We describe a new recovery strategy in which the protein o...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:119:1:79
更新日期:2004-10-01 00:00:00
abstract::6-Tuliposides A (6-PosA) and B (6-PosB) are major secondary metabolites in tulip (Tulipa gesneriana), having an acyl group at the C-6 position of D-glucose. They serve as precursors of the antimicrobial α-methylene-γ-butyrolactones tulipalins A (PaA) and B (PaB). The conversions of 6-PosA/6-PosB to PaA/PaB are catalyz...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-018-2903-3
更新日期:2019-05-01 00:00:00
abstract::An economic evaluation model was developed in the Laboratory of Agricultural Structures (LAS) of the Agricultural University of Athens-the Modified Basic Economic Evaluation Model (MBEEM). This model is an improved version of the original Basic Economic Evaluation Model, available in LAS, and it is used to assess the ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:109:1-3:275
更新日期:2003-04-01 00:00:00
abstract::A spectrophotometric method of measuring oxygenase activity in cell extracts or in zymograms was developed. It is an easy and cheap method that allows spectrophotometric measurement of activity by a colored reaction and reveals activity bands in a polyacrylamide gel electrophoresis (PAGE) gel as brown bands. To prove ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:108:1-3:725
更新日期:2003-04-01 00:00:00
abstract::A comparative proteomic analysis was carried out to explore the molecular mechanisms of responses to cold stress in Phalaenopsis after treated by low temperature (13/8 °C day/night) for 15 days. Differentially expressed proteins were examined using two-dimensional electrophoresis (2-DE) and matrix assisted laser desor...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-014-1345-9
更新日期:2015-01-01 00:00:00
abstract::The performance of cellulase and amylase immobilized on siliceous supports was investigated. Enzyme uptake onto the support depended on the enzyme source and immobilization conditions. For amylase, the uptake ranged between 20 and 60%, and for cellulase, 7-10%. Immobilized amylase performance was assessed by batch kin...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:113:1-3:251
更新日期:2004-04-01 00:00:00
abstract::(R)-2-Phenoxy-propionic acid methyl ester (PPAM) is an important chiral precursor of aryloxy phenoxy propionate herbicides. The covalent immobilization of lipase from Aspergillus oryzae WZ007 and the catalysis of enantioselective (R, S)-PPAM resolution by the immobilized A. oryzae lipase (AOL) were investigated in thi...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-019-03145-4
更新日期:2020-03-01 00:00:00
abstract::Enzymatic hydrolysis of cellulosic raw materials to produce nutrient broths for microbiological synthesis of ethanol and other valuable products is an important field of modern biotechnology. Biotechnological processing implies the selection of an effective pretreatment technique for raw materials. In this study, the ...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1938-y
更新日期:2016-03-01 00:00:00
abstract::Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from ei...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-010-9099-5
更新日期:2011-04-01 00:00:00
abstract::Aroma volatiles in Basmati-370, Ambemohar-157 (non-basmati scented), and IR-64 (non-scented) rice cultivars were qualitatively and quantitatively analyzed at vegetative and maturity stages to study their differential accumulation using headspace solid-phase microextraction, followed by gas chromatography mass spectrom...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-015-1898-2
更新日期:2016-02-01 00:00:00
abstract::When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of de...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-010-9112-z
更新日期:2011-05-01 00:00:00
abstract::Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1385/abab:125:3:159
更新日期:2005-06-01 00:00:00
abstract::Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009. Using new expression tools developed at...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-010-9070-5
更新日期:2011-03-01 00:00:00
abstract::Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, co...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-007-0048-x
更新日期:2007-12-01 00:00:00
abstract::Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on sub...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-016-2131-7
更新日期:2016-10-01 00:00:00