Characterization and performance of immobilized amylase and cellulase.

abstract：:L-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the L-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The im...

journal_title：Applied biochemistry and biotechnology

authors： Kim KR,Seo ES,Oh DK

更新日期：2014-01-01 00:00:00

abstract：:This work aimed at setting up a fully instrumented, laboratory-scale bioreactor enabling anaerobic valorization of solid substrates through hydrogen and/or volatile fatty acid (VFA) production using mixed microbial populations (consortia). The substrate used was made of meat-based wastes, especially from slaughterhous...

journal_title：Applied biochemistry and biotechnology

authors： Pessiot J,Nouaille R,Jobard M,Singhania RR,Bournilhas A,Christophe G,Fontanille P,Peyret P,Fonty G,Larroche C

更新日期：2012-07-01 00:00:00

abstract：:This study presents the production of bacterial cellulose (BC) by a bacterium isolated from a rotten fruit and its process optimization. Here, isolation and screening of potent cellulose producers were carried out from different natural sources, viz., soil, rotten fruits, and vegetables and vinegar. A total of 200 bac...

journal_title：Applied biochemistry and biotechnology

authors： Jahan F,Kumar V,Rawat G,Saxena RK

更新日期：2012-07-01 00:00:00

abstract：:Pretreatment-induced structural alteration is critical in influencing the rate and extent of enzymatic saccharification of lignocellulosic biomass. The present work has investigated structural features of rice straw pretreated by hot-compressed water (HCW) from 140 to 240 °C for 10 or 30 min and enzymatic hydrolysis p...

journal_title：Applied biochemistry and biotechnology

authors： Yu G,Yano S,Inoue H,Inoue S,Wang J,Endo T

更新日期：2014-11-01 00:00:00

abstract：:Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...

journal_title：Applied biochemistry and biotechnology

authors： Rondags E,Halliday E,Marc I

更新日期：1998-02-01 00:00:00

abstract：:A highly sensitive, selective, reversible, and reusable glucose sensor is developed by using molecularly imprinted polymer-based artificial receptors onto interdigital transducer. Sensor receptors were synthesized through bulk imprinting technology by using styrene as monomer, ethylene glycol dimethacrylate (EGDMA) as...

journal_title：Applied biochemistry and biotechnology

authors： Asghar N,Mustafa G,Yasinzai M,Al-Soud YA,Lieberzeit PA,Latif U

更新日期：2019-12-01 00:00:00

abstract：:A new method without any special devices for direct transformation of linear gene cassettes was developed. Its feasibility was verified through 5'-fluorescent dye (fluorescein isothiocyanate, FITC)-labeled fluorescent tracing and transient expression of a gus reporter gene. Minimal linear gene cassettes, containing ne...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YQ,Yang J,Xu FP,An LJ,Liu JF,Chen ZW

更新日期：2009-12-01 00:00:00

abstract：:Two protocols for functionalization of glass supports with hexaethylene glycol (HEG)-linked oligonucleotides were developed. The first method (standard amidite protocol) made use of the 2-cyanoethyl-phosphoramidite derivative of 4,4'-dimethoxytrityl-protected HEG. This was first coupled to the support by standard soli...

journal_title：Applied biochemistry and biotechnology

authors： Sojka B,Piunno PAE,Wust CC,Krull UJ

更新日期：2000-10-01 00:00:00

abstract：:The ability of non-living biomass of an arsenic-hypertolerant Bacillus cereus strain SZ2 isolated from a gold mining environment to adsorb As (III) from aqueous solution in batch experiments was investigated as a function of contact time, initial As (III) concentration, pH, temperature and biomass dosage. Langmuir mod...

journal_title：Applied biochemistry and biotechnology

authors： Mohd Bahari Z,Ali Hamood Altowayti W,Ibrahim Z,Jaafar J,Shahir S

更新日期：2013-12-01 00:00:00

abstract：:Search for efficient low-cost substrate/additives are gaining significant impetus in bacterial cellulose (BC) production. Makgeolli sludge (a traditional Korean wine distillery waste) is enriched with organic acid, alcohol, and sugar. Using makgeolli sludge filtrate (MSF) and Hestrin-Schramm (HS) medium (g/l of distil...

journal_title：Applied biochemistry and biotechnology

authors： Hyun JY,Mahanty B,Kim CG

更新日期：2014-04-01 00:00:00

abstract：:Volatile C6-aldehydes are the main contributors to the characteristic odor of plants known as "green note" and are widely used by the flavor industry. Biotechnological processes were developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants. Recombinant hydroperoxide lyases (HPLs) constit...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Mariani M,de Caraffa VB,Gambotti C,Vincenti S,Desjobert JM,Muselli A,Costa J,Berti L,Maury J

更新日期：2016-06-01 00:00:00

abstract：:Environmental conditions affect the production of extracellular polysaccharide by Azotobacter chroococcum ATCC 4412. Production of exocellular polymer from a variety of carbon sources depended on the air flow rate. A high sucrose concentration in medium (8%) markedly favored expopolysaccharide production, which reache...

journal_title：Applied biochemistry and biotechnology

authors： De la Vega MG,Cejudo FJ,Paneque A

更新日期：1991-09-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) produced by a biofilm-producing bacterium Acinetobacter junii BB1A were characterized. Purified EPS on analysis revealed neutral sugar (73.21 %), uronic acid (10.12 %), amino sugars (0.23 %), α- amino acids (11.13 %), and aromatic amino acids (1.23 %). Infrared spectrometry rev...

journal_title：Applied biochemistry and biotechnology

authors： Yadav KK,Mandal AK,Sen IK,Chakraborti S,Islam SS,Chakraborty R

更新日期：2012-11-01 00:00:00

abstract：:The separation and purification of biologically functional molecules (e.g., proteins, antibodies, peptides, hormones, low molecular weight biologicals) is of fundamental importance to biotechnology. Affinity separations have become a particularly attractive method for bioseparations due to their high degree of selecti...

journal_title：Applied biochemistry and biotechnology

authors： Dordick JS

更新日期：1991-01-01 00:00:00

abstract：:Aliphatic chlorinated compounds, such as trichloroethylene (TCE) and tetrachloroethylene (PCE), are major contaminants of ground water. A single-pass packed-bed bioreactor was utilized to study the biodegradation of organic waste mixtures consisting of PCE, TCE, and other short-chain chlorinated organics. The bioreact...

journal_title：Applied biochemistry and biotechnology

authors： Lackey LW,Phelps TJ,Bienkowski PR,White DC

更新日期：1993-04-01 00:00:00

abstract：:We applied a ribosome display technique to a mouse single chain variable fragment (scFv) library to select scFvs specific for the inducible costimulator (ICOS). mRNA was isolated from the spleens of BALB/c mice immunized with ICOS protein. Heavy and κ chain genes (VH and κ) were amplified separately by reverse transcr...

journal_title：Applied biochemistry and biotechnology

authors： Pan Y,Mao W,Liu X,Xu C,He Z,Wang W,Yan H

更新日期：2012-11-01 00:00:00

abstract：:Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enzyme properties by i...

journal_title：Applied biochemistry and biotechnology

authors： Wei J,Zhou Y,Xu T,Lu B

更新日期：2010-09-01 00:00:00

abstract：:1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...

journal_title：Applied biochemistry and biotechnology

authors： Pornwongthong P,Mulchandani A,Gedalanga PB,Mahendra S

更新日期：2014-05-01 00:00:00

abstract：:Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains...

journal_title：Applied biochemistry and biotechnology

authors： Li SW,Li M,Song HP,Feng JL,Tai XS

更新日期：2011-10-01 00:00:00

abstract：:The effects of oxygen limitation on xylose fermentation of Neurospora crassa AS3.1602 were studied using batch cultures. The maximum yield of ethanol was 0.34 g/g at oxygen transfer rate (OTR) of 8.4 mmol/L.h. The maximum yield of xylitol was 0.33 g/g at OTR of 5.1 mmol/L.h. Oxygen limitation greatly affected mycelia ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang Z,Qu Y,Zhang X,Lin J

更新日期：2008-03-01 00:00:00

abstract：:A new process for enzymatic synthesis of biodiesel at high water content (10-20%) with 96% conversion by lipase from Candida sp. 99-125 was studied. The lipase, a no-position-specific lipase, was immobilized by a cheap cotton membrane and the membrane-immobilized lipase could be used at least six times with high conve...

journal_title：Applied biochemistry and biotechnology

authors： Tan T,Nie K,Wang F

更新日期：2006-02-01 00:00:00

abstract：:A novel expression system of Klebsiella pneumoniae was developed in order to improve 1,3-propanediol (1,3-PD) production using a K. pneumoniae-Escherichia coli shuttle vector pET28a consisting of the kanamycin-resistance gene promoter Pkan. The recombinant plasmid pETPkan-cat carrying the chloramphenicol acetyltransfe...

journal_title：Applied biochemistry and biotechnology

authors： Ma Z,Rao Z,Zhuge B,Fang H,Liao X,Zhuge J

更新日期：2010-09-01 00:00:00

abstract：:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was a...

journal_title：Applied biochemistry and biotechnology

authors： Lianghua T,Liming X,Min S,Huaying G

更新日期：2007-08-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT, EC 2.6.1.1) was bound on CNBr-activated Sepharose and the effects of immobilization on the maximum velocity, biologically active pyridoxal-5'-phosphate (PLP), and transaminationable active centers were studied. By comparing these parameters of soluble and immobilized enzyme the facto...

journal_title：Applied biochemistry and biotechnology

authors： Kurkijärvi K,Korpela T

更新日期：1983-04-01 00:00:00

abstract：:Low-cost dried yeasts immobilized on delignified brewers' spent grains for use in wine making and brewing were produced by simple thermal drying techniques. To optimize the thermal drying process, vacuum and air stream conditions were examined. Drying of thin layers of the biocatalysts was performed at low (30-38 degr...

journal_title：Applied biochemistry and biotechnology

authors： Tsaousi K,Koutinas AA,Bekatorou A,Loukatos P

更新日期：2010-09-01 00:00:00

abstract：:Biosorption is a potential tool for the extraction of metals from contaminated water and recovery of precious metals, which is a convenient alternative to conventional processes. In the present study, molybdenum recovery by Acidithiobacillus ferrooxidans strain ZT-94 was evaluated. Additionally, the effects of pH init...

journal_title：Applied biochemistry and biotechnology

authors： Kasra-Kermanshahi R,Tajer-Mohammad-Ghazvini P,Bahrami-Bavani M

更新日期：2020-11-17 00:00:00

abstract：:Proteins that are important indicators of physiological or pathological states may contribute to the early diagnosis of disease, which may provide a basis for identifying the underlying mechanism of disease development. Serum, contains an abundance of proteins, offers an easy and inexpensive approach for disease detec...

journal_title：Applied biochemistry and biotechnology

authors： Zhang AH,Sun H,Yan GL,Han Y,Wang XJ

更新日期：2013-06-01 00:00:00

abstract：:Degraded gelatin (Gel) and oxidized corn starch (OCS) as abundant, recyclable, and biodegradable materials can be applied to agricultural production, which has been investigated in this research. Firstly, the prepared oxidized corn starch-gelatin (OCS-Gel) composite material was characterized through a Fourier transfo...

journal_title：Applied biochemistry and biotechnology

authors： Dang X,Shan Z,Chen H

更新日期：2016-11-01 00:00:00

abstract：:A green synthesis of iron oxide nanoparticles (FeNPs) was developed using Murraya koenigii leaf extract as reducing and stabilizing agent. UV-vis spectra show that the absorption band centred at a wavelength of 277 nm which corresponds to the surface plasmon resonances of synthesized FeNPs. Fourier transform infrared ...

journal_title：Applied biochemistry and biotechnology

authors： Mohanraj S,Kodhaiyolii S,Rengasamy M,Pugalenthi V

更新日期：2014-05-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00