Abstract:
:Mouse CXCL14/BRAK is a monocyte-selective chemokine which is expressed in almost all normal tissues. A flood of reports on its new functions of tumor suppression and fat metabolism modulation has left CXCL14 a potential therapeutic candidate for these diseases. Therefore, a simple accessible method is on demand for large-scale production of recombinant mouse CXCL14 protein for in vivo animal studies. Here, we introduce an efficient method for large-scale production of recombinant mouse CXCL14, by which an 18-mg protein is produced from 2-L Escherichia coli culture with good bioactivity and low level of endotoxin.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Li J,Gao J,Sah S,Satyal U,Zhang R,Han W,Yu Ydoi
10.1007/s12010-011-9218-ysubject
Has Abstractpub_date
2011-08-01 00:00:00pages
1366-75issue
8eissn
0273-2289issn
1559-0291journal_volume
164pub_type
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