Heterologous expression characteristics of Trichoderma viride endoglucanase V in the silkworm, Bombyx mori L.

Abstract:

:Efficient degradation of cellulose needs a synergistic reaction of the cellulolytic enzymes, which include exoglucanases, endoglucanases, and β-1,4-glucosidase. In this study, we used an improved Bac-to-Bac/BmNPV baculovirus expression system, which lacks the virus-encoded chitinase cathepsin (v-cath) genes of Bombyx mori nucleopolyhedrovirus (BmNPV), to express the endoglucanase V (EG V) gene from Trichoderma viride in silkworm BmN cells and silkworm larvae, and analyzed the characteristics of the recombinant enzyme in silkworm larvae. The result showed that an around 36-kDa protein was visualized in BmN cells at 48 h after the second-generation recombinant mBacmid/BmNPV/EG V baculovirus infection. The crude enzyme extract from the recombinant baculoviruses-infected silkworms exhibited a significant maximum activity at the environmental condition of pH 5.0 and a temperature of 50 °C, and increased 39.86% and 37.76% compared with that from blank mBacmid/BmNPV baculovirus-infected silkworms and normal silkworms, respectively. It was stable at pH range from 5.0 to 10.0 and at temperature range from 40 to 60 °C. The availability of large quantities of EG V that the silkworm provides might greatly facilitate the future research and the potential application in industries.

authors

Li XH,Wang MX,Zhang P,Hu JB,Sun CG,Liu XJ,Zhou F,Niu YS,Malik FA,Bhaskar R,Yang HJ,Miao YG

doi

10.1007/s12010-011-9291-2

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

728-36

issue

2

eissn

0273-2289

issn

1559-0291

journal_volume

165

pub_type

杂志文章
  • In Vivo Evaluation of the Toxic Effect of Ethyl Acetate Extracts of Marine Antibiotic Resistance Pseudomonas Species Derived from the Red Sea.

    abstract::Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2553-x

    authors: El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

    更新日期:2018-01-01 00:00:00

  • Saccharification of marine microalgae using marine bacteria for ethanol production.

    abstract::The saccharification of marine microalgae using amylase from marine bacteria in saline conditions was investigated. An amylase-producing bacterium, Pseudoalterimonas undina NKMB 0074 was isolated and identified. The green microalga NKG 120701 was determined to have the highest concentration of intracellular carbohydra...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:105:1-3:247

    authors: Matsumoto M,Yokouchi H,Suzuki N,Ohata H,Matsunaga T

    更新日期:2003-04-01 00:00:00

  • Biological N removal from wastes generated from amine-based CO2 capture: case monoethanolamine.

    abstract::Large-scale amine-based CO(2) capture will generate waste containing large amounts of ammonia, in addition to contaminants such as the actual amine as well as degradation products thereof. Monoethanolamine (MEA) has been a dominant amine applied so far in this context. This study reveals how biological N removal can b...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-0075-0

    authors: Hauser I,Colaço AB,Skjæran JA,Einbu A,Ostgaard K,Svendsen HF,Cervantes FJ

    更新日期:2013-02-01 00:00:00

  • Combining enzymatic esterification with conventional alkaline transesterification in an integrated biodiesel process.

    abstract::An integrated biodiesel process that combines enzymatic esterification and alkaline transesterification is suggested. With focus on the enzymatic step, the paper provides proof of concept and suggestions for further process development. Hence, palm fatty acid distillate (PFAD) has been enzymatically converted to fatty...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9095-9

    authors: Brask J,Damstrup ML,Nielsen PM,Holm HC,Maes J,De Greyt W

    更新日期:2011-04-01 00:00:00

  • Isolation, purification, and characterization of avian antimicrobial glycopeptide from the posterior salivary gland of Sepia pharaonis.

    abstract::A proteinaceous glycopeptide was isolated from the posterior salivary gland (PSG) of Sepia pharaonis by gel (Sephadex G-100) filtration chromatography and purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Among the collected fractions, fraction 12 showed a retention time (RT) of 31 min. The ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1370-8

    authors: Karthik R,Saravanan R,Ebenezar KK,Sivamalai T

    更新日期:2015-02-01 00:00:00

  • Viramidine-Loaded Galactosylated Nanoparticles Induce Hepatic Cancer Cell Apoptosis and Inhibit Angiogenesis.

    abstract::Current estimates indicate that hepatocarcinoma is the leading cause of death globally. There is interest in utilizing nanomedicine for cancer therapy to overcome side effects of chemo-interventions. Ribavirin, an antiviral nucleoside inhibitor, accumulates inside red blood cells, causing anemia. Its analog, viramidin...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03090-2

    authors: Abd-Rabou AA,Bharali DJ,Mousa SA

    更新日期:2020-01-01 00:00:00

  • Biotechnological production of xylitol from agroindustrial residues. Evaluation of bioprocesses.

    abstract::Batch, fed-batch, and semicontinuous fermentation processes were used for the production of xylitol from sugarcane bagasse hemicellulosic hydrolysate. The best results were achieved by the semicontinuous fermentation process: a xylitol yield of 0.79 g/g with an efficiency of 86% and a volumetric productivity of 0.66 g...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920197

    authors: Rodrigues DC,Silva SS,Prata AM,Felipe MG

    更新日期:1998-04-01 00:00:00

  • Enhancement of long-chain fatty acid production in Escherichia coli by coexpressing genes, including fabF, involved in the elongation cycle of fatty acid biosynthesis.

    abstract::The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9987-y

    authors: Lee S,Lee S,Yoon YJ,Lee J

    更新日期:2013-01-01 00:00:00

  • Polysaccharide hydrolase folds diversity of structure and convergence of function.

    abstract::Polysaccharide glycosyl hydrolases are a group of enzymes that hydrolyze the glycosidic bond between carbohydrates or between a carbohydrate and a noncarbohydrate moiety. Here we illustrate that traditional schemes for grouping enzymes, such as by substrate specificity or by organism of origin, are not appropriate whe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920433

    authors: Himmel ME,Karplus PA,Sakon J,Adney WS,Baker JO,Thomas SR

    更新日期:1997-04-01 00:00:00

  • High photosynthetic productivity of green microalga Chlorella sorokiniana.

    abstract::The batch culture of a newly isolated strain of a green microalga, Chlorella sorokiniana, was carried out using a conical helical tubular photobioreactor. The isolate was capable of good growth at 40 degrees C under an airstream enriched with 10% CO2. The maximum photosynthetic productivity was 34.4 g of dry biomass/(...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:87:3:203

    authors: Morita M,Watanabe Y,Saiki H

    更新日期:2000-06-01 00:00:00

  • Soybean hulls pretreated using thermo-mechanical extrusion--hydrolysis efficiency, fermentation inhibitors, and ethanol yield.

    abstract::Soybean hulls were subjected to thermo-mechanical extrusion pretreatment at various in-barrel moisture contents and screw speeds. Extrusion degraded the lignocellulosic structure and enhanced enzymatic hydrolysis of soybean hulls, with up to 155% increase in glucose yield as compared to untreated substrate. Greater gl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9449-y

    authors: Yoo J,Alavi S,Vadlani P,Behnke KC

    更新日期:2012-02-01 00:00:00

  • Development and application of a system for analysis of mixed cultures of microorganisms.

    abstract::Development and application of a system for real-time quantitative assessment of individual cell activities in a mixed culture system was investigated. This was based on a concept that the activities of individual cells in a mixed culture can be assessed if the cells are physically separated (in separate compartments)...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:80:1:51

    authors: Tanaka H,Ebata T,Kuwahara I,Matsuo M,Ogbonna JC

    更新日期:1999-04-01 00:00:00

  • Protective Role of Tetrahydrocurcumin: an Active Polyphenolic Curcuminoid on Cadmium-InducedOxidative Damage in Rats.

    abstract::In the present work, protective effect of tetrahydrocurcumin (THC) against oxidative damages in cadmium (Cd)-induced toxicity in rats was evaluated. Cd is an important environmental and industrial toxicant that affects almost all the organs, especially liver. Liver is the major organ responsible for the metabolism and...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-017-2430-7

    authors: Ramakrishnan R,Elangovan P,Pari L

    更新日期:2017-09-01 00:00:00

  • Global Transcriptome Profiling of Enterobacter Strain NRS-1 in Response to Hydrogen Peroxide Stress Treatment.

    abstract::Microbes are often subjected to oxidative stress in nature that badly affects their growth rate and viability. Although the response of microbes against oxidative stress has been characterized at the chemical, physiological, and molecular levels, the mechanism of gene-regulation network adaptations of bacteria in resp...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03313-x

    authors: Fei YY,Bhat JA,Gai JY,Zhao TJ

    更新日期:2020-08-01 00:00:00

  • Molecular cloning and characterization of the light-harvesting chlorophyll a/b gene from the pigeon pea (Cajanus cajan).

    abstract::Light-harvesting chlorophyll a/b-binding proteins (LHCB) have been implicated in the stress response. In this study, a gene encoding LHCB in the pigeon pea was cloned and characterized. Based on the sequence of a previously obtained 327 bp Est, a full-length 793 bp cDNA was cloned using the rapid amplification of cDNA...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1825-6

    authors: Qiao G,Wen XP,Zhang T

    更新日期:2015-12-01 00:00:00

  • Abzyme generation using an anti-idiotypic antibody as the "internal image" of an enzyme active site.

    abstract::Since the two reports published in 1986 by the laboratories of R. Lerner and P. G. Schultz, it has been clearly established that antibodies may be induced to act as catalysts in numerous chemical reactions. In all cases, catalytic antibodies were elicited using a substrate-based approach. In the present article, we pr...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/BF02787937

    authors: Friboulet A,Izadyar L,Avalle B,Roseto A,Thomas D

    更新日期:1994-05-01 00:00:00

  • Improvement of zinc bioaccumulation and biomass yield in the mycelia and fruiting bodies of Pleurotus florida cultured on liquid media.

    abstract::The effect of different concentrations of zinc on the bioaccumulation of zinc and biomass yield in both mycelium and fruiting body of Pleurotus florida cultivated in liquid medium was studied. The results showed that the optimum yield of mycelia (11.33 ± 0.44 g/L) and fruiting bodies (7.70 ± 0.19 g/L) dry biomass was ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1510-9

    authors: Poursaeid N,Azadbakht A,Balali GR

    更新日期:2015-04-01 00:00:00

  • Effects of pipe materials on chlorine-resistant biofilm formation under long-term high chlorine level.

    abstract::Drinking water distribution systems are composed of various pipe materials and may harbor biofilms even in the continuous presence of disinfectants. Biofilms formation on five pipe materials (copper (Cu), polyethylene (PE), stainless steel (STS), cast iron (CI), and concrete-coated polycarbonate (CP)) within drinking ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0935-x

    authors: Zhu Z,Wu C,Zhong D,Yuan Y,Shan L,Zhang J

    更新日期:2014-07-01 00:00:00

  • Evaluation of recombinant green fluorescent protein, under various culture conditions and purification with HiTrap hydrophobic interaction chromatography resins.

    abstract::To determine the influence of various culture conditions, transformed cells of Escherichia coli expressing recombinant green fluorescent protein (GFPuv) were grown in nine cultures with four variable conditions (storage of inoculated broth at 4 degrees C prior to incubation, agitation speed, isopropyl-beta-D-thiogalac...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:114:1-3:453

    authors: Penna TC,Ishii M,Junior AP,de Oliveira Nascimento L,de Souza LC,Cholewa O

    更新日期:2004-04-01 00:00:00

  • Influence of temperature on performance of an anaerobic sequencing biofilm batch reactor with circulation applied to treatment of low-strength wastewater.

    abstract::The effect of temperature on the performance of an anaerobic sequencing biofilm batch reactor (ASBBR) with liquid-phase recirculation was assessed. Assays were performed using a recirculation velocity of 0.20 cm/s, 8-h cycles, and an average treated synthetic wastewater volume of 2 L/cycle with a concentration of 500 ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02686017

    authors: Agibert SA,Moreira MB,Ratusznei SM,Rodrigues JA,Zaiat M,Foresti E

    更新日期:2007-02-01 00:00:00

  • Xanthan gum production by Xanthomonas campestris w.t. fermentation from chestnut extract.

    abstract::Xanthomonas campestris w.t. was used for production of xanthan gum in fermentations with chestnut flour for the first time. Fermentations were carried out with either chestnut flour or its soluble sugars (33.5%) and starch (53.6%), respectively, at 28 degrees C and 200 rpm at initial pH 7.0 in flasks. The effect of ag...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:82:3:175

    authors: Liakopoulou-Kyriakides M,Psomas SK,Kyriakidis DA

    更新日期:1999-12-01 00:00:00

  • Ammonium hydroxide detoxification of spruce acid hydrolysates.

    abstract::When dilute-acid hydrolysates from spruce are fermented to produce ethanol, detoxification is required to make the hydrolysates fermentable at reasonable rates. Treatment with alkali, usually by overliming, is one of the most efficient approaches. Several nutrients, such as ammonium and phosphate, are added to the hyd...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:124:1-3:0911

    authors: Alriksson B,Horváth IS,Sjöde A,Nilvebrant NO,Jönsson LJ

    更新日期:2005-04-01 00:00:00

  • Efficient Secretory Overexpression of Endoinulinase in Escherichia coli and the Production of Inulooligosaccharides.

    abstract::Endoinulinase production was achieved by heteroexpression of endoinulinase-encoding gene from Aspergillus ficuum which is an eukaryotic organism in Escherichia coli BL21 (DE3). Further analysis demonstrated that the native signal peptide existed in inu2 gene lowered the enzyme expression level. To realize extracellula...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2037-4

    authors: Wang P,Ma J,Zhang Y,Zhang M,Wu M,Dai Z,Jiang M

    更新日期:2016-07-01 00:00:00

  • A Novel Cellobiohydrolase I (CBHI) from Penicillium digitatum: Production, Purification, and Characterization.

    abstract::A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03307-9

    authors: Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

    更新日期:2020-09-01 00:00:00

  • A different method of measuring and detecting monoand dioxygenase activities: key enzymes in hydrocarbon biodegradation.

    abstract::A spectrophotometric method of measuring oxygenase activity in cell extracts or in zymograms was developed. It is an easy and cheap method that allows spectrophotometric measurement of activity by a colored reaction and reveals activity bands in a polyacrylamide gel electrophoresis (PAGE) gel as brown bands. To prove ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:108:1-3:725

    authors: Zazueta-Sandoval R,Novoa VZ,Jiménez HS,Ortiz RC

    更新日期:2003-04-01 00:00:00

  • Laccase-catalyzed oxidative polymerization of phenolic compounds.

    abstract::Enzymatic polymerization of phenolic compounds (catechol, resorcinol, and hydroquinone) was carried out using laccase. The mechanism of polymerization and the structures of the polymers were evaluated in terms of UV-Vis and Fourier transform infrared spectroscopy. The molecular weights of the produced polyphenols were...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0463-0

    authors: Sun X,Bai R,Zhang Y,Wang Q,Fan X,Yuan J,Cui L,Wang P

    更新日期:2013-12-01 00:00:00

  • Core-Shell Encapsulation of Lipophilic Substance in Jelly Fig (Ficus awkeotsang Makino) Polysaccharides Using an Inexpensive Acrylic-Based Millifluidic Device.

    abstract::The polysaccharides extracted from the achenes of jelly fig, Ficus awkeotsang Makino, were mainly composed of low methyl pectin and used as a novel shell material for encapsulating lipophilic bioactives in the core of microcapsule. The polysaccharide microcapsules with oil core were prepared using a novel acrylic-base...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03209-5

    authors: Ponrasu T,Yang RF,Chou TH,Wu JJ,Cheng YS

    更新日期:2020-05-01 00:00:00

  • Study on the characterization of lead (II) biosorption by fungus Aspergillus parasiticus.

    abstract::The lead (II) biosorption potential of Aspergillus parasiticus fungal biomass has been investigated in a batch system. The initial pH, biosorbent dosage, contact time, initial metal ion concentrations and temperature were studied to optimize the biosorption conditions. The maximum lead (II) biosorption capacity of the...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-9032-8

    authors: Akar T,Tunali S,Cabuk A

    更新日期:2007-03-01 00:00:00

  • Enhancement of sophorolipid production of Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 by low-energy ion beam implantation.

    abstract::To meet the increasing demands of sophorolipids as biosurfactants and bioactive compounds, it is necessary to obtain higher and more specific sophorolipid-producing strains. One sophorolipid-producing strain, Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 (Y(2A)), was mutated by low-energy nitrogen ion beam im...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-9664-1

    authors: Li H,Ma X,Shao L,Shen J,Song X

    更新日期:2012-06-01 00:00:00

  • Chitosan-modified carbon nanotubes-based platform for low-density lipoprotein detection.

    abstract::We have fabricated an immunosensor based on carbon nanotubes and chitosan (CNT-CH) composite for detection of low density lipoprotein (LDL) molecules via electrochemical impedance technique. The CNT-CH composite deposited on indium tin oxide (ITO)-coated glass electrode has been used to covalently interact with anti-a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1179-5

    authors: Ali MA,Singh N,Srivastava S,Agrawal VV,John R,Onoda M,Malhotra BD

    更新日期:2014-10-01 00:00:00