Development and application of a system for analysis of mixed cultures of microorganisms.

abstract：:Bioethanol is considered a potential liquid fuel that can be produced from biomass by fermentation and distillation. Although most of the water is removed by distillation, the purity of ethanol is limited to 95-96 % due to the formation of a low-boiling point, water-ethanol azeotrope. To improve the use of ethanol as ...

journal_title：Applied biochemistry and biotechnology

authors： Tang B,Bi W,Row KH

更新日期：2013-02-01 00:00:00

abstract：:The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased su...

journal_title：Applied biochemistry and biotechnology

authors： Sousa CA,Hanselaer S,Soares EV

更新日期：2015-01-01 00:00:00

abstract：:Milk is a natural nutraceutical produced by mammals. The nanovesicles of milk play a role in horizontal gene transfer and confer health-benefits to milk consumers. These nanovesicles contain miRNA, mRNA, and proteins which mediate the intercellular communication. In this work, we isolated and characterized the buffalo...

journal_title：Applied biochemistry and biotechnology

authors： Baddela VS,Nayan V,Rani P,Onteru SK,Singh D

更新日期：2016-02-01 00:00:00

abstract：:The cellulosome is a supramolecular multienzyme complex formed via species-specific interactions between the cohesin modules of scaffoldin proteins and the dockerin modules of a wide variety of polysaccharide-degrading enzymes. Here, we report a comparative analysis of cellulosomes prepared from the thermophilic anaer...

journal_title：Applied biochemistry and biotechnology

authors： Shinoda S,Kurosaki M,Kokuzawa T,Hirano K,Takano H,Ueda K,Haruki M,Hirano N

更新日期：2019-03-01 00:00:00

abstract：:For efficiently enhancing the activity of isoeugenol monooxygenase, a whole cell overproducing active aggregate IEM720-18A was successfully fabricated via the fusion of amphiphilic short peptide 18A (EWLKAFYEKVLEKLKELF) and isoeugenol monooxygenase and then efficiently expressed in E. coli BL21 (DE3). Such resulting b...

journal_title：Applied biochemistry and biotechnology

authors： Zhao L,Jiang Y,Fang H,Zhang H,Cheng S,Rajoka MSR,Wu Y

更新日期：2019-10-01 00:00:00

abstract：:The coarse perlite 40-80 mesh was selected as an immobilizing material and put into a packed bed reactor (PBR) to continuously convert maltose to isomalto-oligosaccharides (IMOs). The PBR was prepared by mixing the thermo-inactivated cells (TIC) from Aspergillus niger J2 strain with the coarse perlite, then the mixtur...

journal_title：Applied biochemistry and biotechnology

authors： Huang Z,Li Z,Su Y,Zhu Y,Zeng W,Chen G,Liang Z

更新日期：2018-08-01 00:00:00

abstract：:The purpose of this investigation was to study the effect of Streptomyces erumpens cells immobilized in various matrices, i.e., agar-agar, polyacrylamide, and luffa (Luffa cylindrica L.) sponge for production of alpha-amylase. Luffa sponge was found to be 21% and 51% more effective in enzyme yield than agar-agar and p...

journal_title：Applied biochemistry and biotechnology

authors： Kar S,Swain MR,Ray RC

更新日期：2009-02-01 00:00:00

abstract：:The present work evaluates the phenol degradative performance of microalgae Chlorella pyrenoidosa. High-performance liquid chromatography (HPLC) analysis showed that C. pyrenoidosa degrades phenol completely up to 200 mg/l. It could also metabolize phenol in refinery wastewater. Biokinetic parameters obtained are the ...

journal_title：Applied biochemistry and biotechnology

authors： Das B,Mandal TK,Patra S

更新日期：2015-07-01 00:00:00

abstract：:Outdoor production of microalgae could be set on a sound industrial basis if solar energy were utilized at a much higher efficiency than presently obtained. Many types of photobioreactors have been developed in the past in an attempt to answer this challenge, but their photosynthetic efficiency has been rather similar...

journal_title：Applied biochemistry and biotechnology

authors： Richmond A,Qiang H

更新日期：1997-04-01 00:00:00

abstract：:Observation of the ultrastructural and intracellular chemical changes of CaCl2-tolerant strains is important both for understanding their adaptation mechanism under high salt stress and for providing theoretical basis of their application in treating high-CaCl2 wastewaters. A novel strain V 430 isolated by us has been...

journal_title：Applied biochemistry and biotechnology

authors： Xin X,Wang Y

更新日期：2007-09-01 00:00:00

abstract：:The bioaugmentation of petroleum-contaminated soil using Enterobacter cloacae was profiled from the evolution of microbial community, soil dehydrogenase activity, to the degradation of petroleum contaminants. The seeding and proliferation of inoculant and the consequential microbial community were monitored by denatur...

journal_title：Applied biochemistry and biotechnology

authors： Wu Z,Dong H,Zou L,Lu D,Liu Z

更新日期：2011-08-01 00:00:00

abstract：:Androstenedione (AD) is a steroid intermediate used in the pharmaceutical industry for the production of several important anabolic drugs. An important route for producing AD is by the microbial transformation of sterols. Microbes capable of efficiently transforming sterols to AD are few and newer strains need to be i...

journal_title：Applied biochemistry and biotechnology

authors： Malaviya A,Gomes J

更新日期：2009-08-01 00:00:00

abstract：:The microheterogeneous native amylolytic complex secreted by the isolate A6 of Lactobacillus plantarum revealed a selective enzyme specificity loss when submitted to a limited proteolysis under a suboptimum pH condition. A clear electrophoretic profile change toward just one shorter, more acidic, and equally active po...

journal_title：Applied biochemistry and biotechnology

authors： Florêncio JA,Eiras-Stofella DR,Soccol CR,Raimbault M,Guyot JP,Fontana JD

更新日期：2000-04-01 00:00:00

abstract：:Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

journal_title：Applied biochemistry and biotechnology

authors： Ferreira MS,De Andrade AV,Kennedy JF

更新日期：1991-10-01 00:00:00

abstract：:Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...

journal_title：Applied biochemistry and biotechnology

authors： Rondags E,Halliday E,Marc I

更新日期：1998-02-01 00:00:00

abstract：:The thermal stability of most nitrile hydratases (NHase) is poor, which has been enhanced to some extent by molecular modifications in several specific regions of the C-terminal domain (C-domain) of β subunit of NHase. Since the C-domain could be present as a naturally separate domain in a few NHases, the whole C-doma...

journal_title：Applied biochemistry and biotechnology

authors： Sun W,Zhu L,Chen X,Wu L,Zhou Z,Liu Y

更新日期：2016-04-01 00:00:00

abstract：:This work investigated the cultivation of Chlorella vulgaris in a thin-film flat plate photobioreactor under outdoor conditions and using digested piggery wastewater as the culture medium. The algal cells were able to adapt quickly to the wastewater and outdoor conditions. A specific growth rate of 0.12 day-1 was obta...

journal_title：Applied biochemistry and biotechnology

authors： Sun ZL,Sun LQ,Chen GZ

更新日期：2019-04-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) produced by a biofilm-producing bacterium Acinetobacter junii BB1A were characterized. Purified EPS on analysis revealed neutral sugar (73.21 %), uronic acid (10.12 %), amino sugars (0.23 %), α- amino acids (11.13 %), and aromatic amino acids (1.23 %). Infrared spectrometry rev...

journal_title：Applied biochemistry and biotechnology

authors： Yadav KK,Mandal AK,Sen IK,Chakraborti S,Islam SS,Chakraborty R

更新日期：2012-11-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:The yeast Saccharomyces cerevisiae has been used to efficiently produce L-malic acid from fumaric acid. Fumarase is responsible for the reversible conversion of fumaric and L-malic acids in the TCA cycle. To investigate the function of mitochondrial and cytoplasmic fumarase isoenzymes in L-malic acid bioconversion, a ...

journal_title：Applied biochemistry and biotechnology

authors： Wang X,Gong CS,Tsao GT

更新日期：1998-04-01 00:00:00

abstract：:A DNA fragment of 2,042 bp containing a novel beta-mannanase gene, man5A, was identified from the genome of the mannan-degrading bacterium Bacillus circulans CGMCC1554. The open reading frame of man5A comprised 978 bp encoding a protein of 326 amino acids with a predicted molecular weight of 32 kDa. The amino acid seq...

journal_title：Applied biochemistry and biotechnology

authors： Yang P,Li Y,Wang Y,Meng K,Luo H,Yuan T,Bai Y,Zhan Z,Yao B

更新日期：2009-10-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00

abstract：:We have described a novel red biochrome, 514 Da in size, produced by solid-state cultivation of a bacterial isolate obtained from garden soil. The growth requirements of the isolate, the chemical characteristics of the biochrome produced, and the application of the biochrome in dying of silk, wool, and cotton fabrics ...

journal_title：Applied biochemistry and biotechnology

authors： Vaidyanathan J,Bhathena-Langdana Z,Adivarekar RV,Nerurkar M

更新日期：2012-01-01 00:00:00

abstract：:Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of...

journal_title：Applied biochemistry and biotechnology

authors： Rath D,Kumar S,Panda S

更新日期：2019-04-01 00:00:00

abstract：:Protein-protein interactions (PPIs) play pivotal roles in most of the biological processes. PPI dysfunctions are therefore associated with disease situations. Mutations often lead to PPI dysfunctions, but there are certain other types of mutations which do not cause any appreciable abnormalities. This second type of m...

journal_title：Applied biochemistry and biotechnology

authors： Ali A,Biswas R,Bhattacharjee S,Nath P,Pan S,Bagchi A

更新日期：2016-09-01 00:00:00

abstract：:Current estimates indicate that hepatocarcinoma is the leading cause of death globally. There is interest in utilizing nanomedicine for cancer therapy to overcome side effects of chemo-interventions. Ribavirin, an antiviral nucleoside inhibitor, accumulates inside red blood cells, causing anemia. Its analog, viramidin...

journal_title：Applied biochemistry and biotechnology

authors： Abd-Rabou AA,Bharali DJ,Mousa SA

更新日期：2020-01-01 00:00:00

abstract：:The antioxidant activity of a novel artificial glutathione peroxidase-like enzyme, selenium-containing glutathione 5-transferase from Lucilia cuprina (seleno-LuGST1-1), was studied by using a ferrous sulfate/ascorbate-induced mitochondrial damage model system. Swelling of mitochondria, lipid peroxidation, and cytochro...

journal_title：Applied biochemistry and biotechnology

authors： Yu H,Liu J,Li J,Zang T,Luo G,Shen J

更新日期：2005-11-01 00:00:00

abstract：:Xylans are major hemicellulose components of plant cell wall which can be hydrolyzed by xylanolytic enzymes. Three forms of endo-β-1,4-xylanases (XynSW1, XynSW2A, and XynSW2B) produced by thermotolerant Streptomyces sp. SWU10 have been reported. In the present study, we described the expression and characterization of...

journal_title：Applied biochemistry and biotechnology

authors： Sukhumsirichart W,Deesukon W,Kawakami T,Matsumoto S,Seesom W,Sakamoto T

更新日期：2014-01-01 00:00:00

abstract：:The changes in total phenolics, flavonoids, tannins, valerenic acid, and antioxidant activity were assessed in 25 populations of Valeriana jatamansi sampled from 1200 to 2775 m asl and four habitat types of Uttarakhand, West Himalaya. Significant (p < 0.05) variations in total phenolics, flavonoids, valerenic acid, an...

journal_title：Applied biochemistry and biotechnology

authors： Jugran AK,Bahukhandi A,Dhyani P,Bhatt ID,Rawal RS,Nandi SK

更新日期：2016-07-01 00:00:00

abstract：:The use of the insect cell/baculovirus expression system for producing recombinant proteins of bacterial, plant, insect, and mammalian origin has become widespread. The popularity of this eukaryotic expression system is due to many factors, including (1) potentially high protein expression levels, (2) ease and speed o...

journal_title：Applied biochemistry and biotechnology

authors： Murhammer DW

更新日期：1991-12-01 00:00:00