Principles for efficient utilization of light for mass production of photoautotrophic microorganisms.

abstract：:Carnosol and carnosic acid are polyphenolic compounds found in rosemary and sage with known anti-oxidant, anti-inflammatory, and anti-microbial properties. Here, we addressed the potential use of carnosol and carnosic acid for in vitro bone tissue engineering applications, specifically depending on their cytotoxic eff...

journal_title：Applied biochemistry and biotechnology

authors： Karadas O,Mese G,Ozcivici E

更新日期：2019-06-01 00:00:00

abstract：:In this presented work, magnetic poly(HEMA-GMA) nanoparticles were synthesized, characterized, and used for immobilization of an anti-leukemic enzyme L-asparaginase. The average particle size of the synthesized magnetic nanoparticles was found to be as 117.5 nm. L-asparaginase was successfully immobilized onto the syn...

journal_title：Applied biochemistry and biotechnology

authors： Orhan H,Aktaş Uygun D

更新日期：2020-08-01 00:00:00

abstract：:This research aimed at developing and designing a slow and targeted delivery of Amphotericin B (AmB) antibiotic by placing three types of shells containing different ratios of biodegradable and biocompatible polymers poly (D, L-lactide)-co-(glycolide) (PLGA), polyethylene glycol (PEG), and polyvinyl pyrrolidone (PVP) ...

journal_title：Applied biochemistry and biotechnology

authors： Sadat Akhavi S,Moradi Dehaghi S

更新日期：2020-06-01 00:00:00

abstract：:The gene encoding a thermostable β-D-xylosidase (GbtXyl43B) from Geobacillus thermoleovorans IT-08 was cloned in pET30a and expressed in Escherichia coli; additionally, characterization and kinetic analysis of GbtXyl43B were carried out. The gene product was purified to apparent homogeneity showing M r of 72 by sodium...

journal_title：Applied biochemistry and biotechnology

authors： Ratnadewi AA,Fanani M,Kurniasih SD,Sakka M,Wasito EB,Sakka K,Nurachman Z,Puspaningsih NN

更新日期：2013-08-01 00:00:00

abstract：:The enzyme 3-hydroxy-3-methyl-glutaryl CoA reductase (HMGR) is a glycoprotein of the endoplasmic reticulum that participates in the mevalonate pathway, the precursor of cholesterol in human and ergosterol in fungi. This enzyme has three domains: transmembrane, binding, and soluble. In this study, we expressed and puri...

journal_title：Applied biochemistry and biotechnology

authors： Andrade-Pavón D,Cuevas-Hernández RI,Trujillo-Ferrara JG,Hernández-Rodríguez C,Ibarra JA,Villa-Tanaca L

更新日期：2017-08-01 00:00:00

abstract：:This study deals with the utilization of chicken feather waste as a substrate for anaerobic digestion and improving biogas production by degradation of the compact structure of the feather keratin. In order to increase the digestibility of the feather, different pretreatments were investigated, including thermal pretr...

journal_title：Applied biochemistry and biotechnology

authors： Forgács G,Lundin M,Taherzadeh MJ,Sárvári Horváth I

更新日期：2013-04-01 00:00:00

abstract：:The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 6...

journal_title：Applied biochemistry and biotechnology

authors： Errasti ME,Natalucci CL,Caffini NO,Rotelli AE,Brullo A,Maras B,Trejo SA,López LMI

更新日期：2018-09-01 00:00:00

abstract：:Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown. In the present study, we cl...

journal_title：Applied biochemistry and biotechnology

authors： Zhang X,Quan G,Wang J,Han H,Chen S,Guo S,Yin H

更新日期：2015-04-01 00:00:00

abstract：:The objective of the present work was to evaluate the metabolic effects induced by the initial glucose concentration (IGC) on the cultivation of Streptococcus zooepidemicus for the production of hyaluronic acid (HA). These effects were monitored along non-controlled pH cultivations, carried out in 250-mL Erlenmeyer fl...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Santana MH

更新日期：2010-11-01 00:00:00

abstract：:The nuclear receptor Rev-erbβ is a potent transcriptional factor whose functional study has been limited by the lack of suitable antibodies against it. To better understand Rev-erbβ's biological roles, we generated five hybridoma cell lines secreting antibodies against human Rev-erbβ in mice immunized with the purifie...

journal_title：Applied biochemistry and biotechnology

authors： Chen F,Li Y,Zhao J,Mao Q,Xia H

更新日期：2017-07-01 00:00:00

abstract：:The kinetic parameters of the oxidant action of the combination of enzyme horseradish peroxidase (HRP) with hydrogen peroxide in the degradation of methylene blue dye were investigated. Twenty-one percent of color removal was obtained at pH 5.0 and temperature of 30 °C. Under these conditions, the kinetic parameters K...

journal_title：Applied biochemistry and biotechnology

authors： Pereira AR,da Costa RS,Yokoyama L,Alhadeff EM,Teixeira LA

更新日期：2014-12-01 00:00:00

abstract：:Alginate lyases have been widely used for the preparation of bioactive alginate oligosaccharides. An alginate lyase AlgL-CD was rationally designed by introducing alkaline amino acid residues near active center to increase activity. One of its mutants E226K presented much higher activity than wild-type AlgL-CD. Substr...

journal_title：Applied biochemistry and biotechnology

authors： Xu X,Zeng D,Wu D,Lin J

更新日期：2021-01-23 00:00:00

abstract：:Maltodextrins and hydrolysates of rice and corn flour of varying dextrose equivalents (DE) have been used as a carbon source for alpha-amylase enzyme production by Bacillus sp. TCRDC-25A. The rate and total enzyme production was higher in maltodextrin media than in cornstarch. The enzyme production increased with incr...

journal_title：Applied biochemistry and biotechnology

authors： Bajpai P,Bajpai PK

更新日期：1991-11-01 00:00:00

abstract：:Forty-four endophytic fungal isolates obtained from marine sponge, Hyrtios erectus, were evaluated and screened for their hydrolase activities. Most of the isolates were found to be prolific producers of hydrolytic enzymes. Only 11 isolates exhibited maximum cellular contents of lipids, rhamnolipids, and protein in th...

journal_title：Applied biochemistry and biotechnology

authors： El-Gendy MMAA,Yahya SMM,Hamed AR,Soltan MM,El-Bondkly AMA

更新日期：2018-07-01 00:00:00

abstract：:A combined process, de-algination followed by enzymatic saccharification, was designed to produce alginate and glucose from Saccharina japonica consecutively. The process conditions of de-algination were optimized separately for each stage of acidification and alkaline extraction. Collectively, the de-algination yield...

journal_title：Applied biochemistry and biotechnology

authors： Ryu HJ,Oh KK

更新日期：2017-05-01 00:00:00

abstract：:This study aimed to investigate fragments derived from human and bovine lactoferrins for ability to inhibit nuclear translocation of HIV-1 integrase. It was shown that human lactoferricin, human lactoferrin 1-11, and bovine lactoferrampin reduced nuclear distribution of HIV-1 integrase. Bovine lactoferrampin could inh...

journal_title：Applied biochemistry and biotechnology

authors： Wang WY,Wong JH,Ip DT,Wan DC,Cheung RC,Ng TB

更新日期：2016-08-01 00:00:00

abstract：:The RNA-guided endonuclease clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) derived from CRISPR systems is a simple and efficient genome-editing technology applied to various cell types and organisms. So far, the extensive approach to detect the cleavage activity of custo...

journal_title：Applied biochemistry and biotechnology

authors： Yang Y,Liu S,Cheng Y,Nie L,Lv C,Wang G,Zhang Y,Hao L

更新日期：2016-10-01 00:00:00

abstract：:Fresh-harvested, air-dried rice straw was pretreated at a water content of 5 g H(2)O/g straw using sodium hydroxide (NaOH) and compared to pretreatment at 10 g H(2)O/g straw by hydrated lime (Ca(OH)(2)). Full factorial experiments including parallel wash-only treatments were completed with both sources of alkali. The ...

journal_title：Applied biochemistry and biotechnology

authors： Cheng YS,Zheng Y,Yu CW,Dooley TM,Jenkins BM,VanderGheynst JS

更新日期：2010-11-01 00:00:00

abstract：:Streptomyces griseorubens JSD-1 is a novel actinomycete that could grow efficiently upon lignin, and the ligninolytic genes active in this biotransformation were expected to be crucial. To investigate the molecular mechanism of utilizing lignin, genome sequencing was carried out to obtain its draft genome, which was d...

journal_title：Applied biochemistry and biotechnology

authors： Feng H,Zhang D,Sun Y,Zhi Y,Mao L,Luo Y,Xu L,Wang L,Zhou P

更新日期：2015-05-01 00:00:00

abstract：:Flavonoids are secondary metabolites synthesized by plants shown to exhibit health benefits such as anti-inflammatory, antioxidant, and anti-tumor effects. Thus, due to the importance of this compound, several enzymes involved in the flavonoid pathway have been cloned and characterized in Escherichia coli. However, th...

journal_title：Applied biochemistry and biotechnology

authors： Zakaria II,Rahman RN,Salleh AB,Basri M

更新日期：2011-09-01 00:00:00

abstract：:A lactate oxidase was purified about 36-fold from a newly screened strain KY6 of gram negative bacterium from soil to yield a homogeneous protein. The native enzyme had a molecular mass of 204 kDa measured by Sephadex G-200 and that of subunit on the SDS-PAGE was found to be 45 kDa. The enzyme was optimally active at ...

journal_title：Applied biochemistry and biotechnology

authors： Xu P,Yano T,Yamamoto K,Suzuki H,Kumagai H

更新日期：1996-03-01 00:00:00

abstract：:Agrobacterium isolated from soil samples produced two extracellular polysaccharides: succinoglycan, an acidic soluble polymer, and curdlan gum, a neutral, insoluble polymer. Maize glucose, cassava glucose, and maize maltose were used in fermentation medium to produce insoluble polysaccharide. Two Agrobacterium sp. str...

journal_title：Applied biochemistry and biotechnology

authors： Portilho M,Matioli G,Zanin GM,de Moraes FF,Scamparini AR

更新日期：2006-03-01 00:00:00

abstract：:Biomass degradation compounds significantly inhibit biochemical conversion of biomass prehydrolysates to biofuels and chemicals, such as lactic acid. To characterize the structure-activity relationship of carbonyl inhibition on lactic acid fermentation, we examined effects of eight carbonyl compounds (furfural, 5-hydr...

journal_title：Applied biochemistry and biotechnology

authors： Li J,Zhu C,Tu M,Han P,Wu Y

更新日期：2015-04-01 00:00:00

abstract：:Lignocellulosic biomass has great potential as a cheap feedstock in biological processes to produce biofuels or chemicals; however, dilute acid pretreatment at high temperatures produces undesirable compounds. Toxicity tests were done with inhibitors in standard media, to predict the growth-limiting effects on thermop...

journal_title：Applied biochemistry and biotechnology

authors： Thomasser C,Danner H,Neureiter M,Saidi B,Braun R

更新日期：2002-04-01 00:00:00

abstract：:The fermentative production of lactic acid from cheese whey and corn steep liquor (CSL) as cheap raw materials was investigated by using Lactobacillus sp. RKY2 in order to develop a cost-effective fermentation medium. Lactic acid yields based on consumed lactose were obtained at more than 0.98 g/g from the medium cont...

journal_title：Applied biochemistry and biotechnology

authors： Kim HO,Wee YJ,Kim JN,Yun JS,Ryu HW

更新日期：2006-04-01 00:00:00

abstract：:The present study was undertaken to evaluate the effect of initial temperature treatment on phytochemical and antioxidant potential of commercially important plant Azadirachta indica A. Juss. The leaves were differentially treated after harvest at temperatures 4, -20, and 110 °C and at room temperature, separately. It...

journal_title：Applied biochemistry and biotechnology

authors： Vats S

更新日期：2016-02-01 00:00:00

abstract：:A protein-engineered beta-lactamase, constructed by site-directed mutagenesis in Escherichia coli (E104M/G238S), and having broadened specificity, was able to degrade cephalosporins of first, second, and third generations. Manipulations of culture conditions allowed an increase in beta-lactamase specific activity by u...

journal_title：Applied biochemistry and biotechnology

authors： García JL,Nuñez CJ,González EG,Osuna J,Soberón X,Galindo E

更新日期：1998-05-01 00:00:00

abstract：:Extracellular polymeric substances (EPS) produced by a biofilm-producing bacterium Acinetobacter junii BB1A were characterized. Purified EPS on analysis revealed neutral sugar (73.21 %), uronic acid (10.12 %), amino sugars (0.23 %), α- amino acids (11.13 %), and aromatic amino acids (1.23 %). Infrared spectrometry rev...

journal_title：Applied biochemistry and biotechnology

authors： Yadav KK,Mandal AK,Sen IK,Chakraborti S,Islam SS,Chakraborty R

更新日期：2012-11-01 00:00:00

abstract：:Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...

journal_title：Applied biochemistry and biotechnology

authors： Bollók M,Réczey K,Zacchi G

更新日期：2000-04-01 00:00:00

abstract：:In this study, the feasibility of introducing redox property to an amphiphilic phospholipid polymer (PMBN) was investigated. The active ester group in the side chain of the polymer was used to react with pyrroloquinoline quinine (PQQ). Redox peaks that corresponded to PQQ redox potentials were observed after the modif...

journal_title：Applied biochemistry and biotechnology

authors： Yu EH,Himuro Y,Takai M,Ishihara K

更新日期：2010-02-01 00:00:00