Abstract：
:Reed plants (Phragmites communis (Linn.) Trin) are hydrophilic perennial grasses growing in fresh and brackish waters. These plants readily adapt to arid and high salinity conditions; however, their resistance mechanism against abiotic stresses, especially high salinity, is largely unknown. In the present study, we cloned a glutathione reductase gene from P. communis and investigated its role in conferring salt tolerance in reed plants. The expression of PhaGR at the transcriptional level was affected by multiple abiotic stresses including NaCl, Cd(2+), heat, cold, PEG 6000, and abscisic acid (ABA). Furthermore, NaCl and Cd(2+) could increase its expressions at the translational level. NaCl and Cd(2+) also increased the biosynthesis of soluble protein and reduced glutathione (GSH). Reed seedlings that were challenged with NaCl showed higher levels of GR activities, which corroborated our gene expression data. The increase in GR possibly increased the salt tolerance of reed plants through GSH production. Thus, PhaGR is a potential target gene in improving the salt tolerance of crops through genetic manipulation.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Zhang X,Quan G,Wang J,Han H,Chen S,Guo S,Yin Hdoi
10.1007/s12010-015-1514-5subject
Has Abstractpub_date
2015-04-01 00:00:00pages
3418-30issue
7eissn
0273-2289issn
1559-0291journal_volume
175pub_type
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