Single-Point Mutation Near Active Center Increases Substrate Affinity of Alginate Lyase AlgL-CD.

abstract：:In the present investigation, thermophilic fungus Rhizomucor pusillus was used to study biotransformation of antihelmintic drug albendazole to produce its active metabolite, albendazole sulfoxide and novel metabolites of commercial interest. A two-stage fermentation procedure was followed for biotransformation of albe...

journal_title：Applied biochemistry and biotechnology

authors： Prasad GS,Girisham S,Reddy SM

更新日期：2011-11-01 00:00:00

abstract：:Proinflammatory cytokines such as tumor necrosis factor (TNF) alpha are well known to inhibit adipocyte differentiation. TNF-alpha triggers ceramide synthesis through binding of TNF-alpha to its p55 receptor. Therefore, ceramide is implicated in many of the multiple signaling pathways initiated by TNF-alpha. In breast...

journal_title：Applied biochemistry and biotechnology

authors： Xu F,Yang CC,Gomillion C,Burg KJ

更新日期：2010-01-01 00:00:00

abstract：:Among 18 psychrophilic strains isolated near the Antarctic Station, the psychrophilic strain Rhodotorula aurantiaca A19 was selected for its ability of growth and gamma-decalactone production at low temperatures. The effects of temperature, initial pH, and castor oil concentration on the growth and gamma-decalactone p...

journal_title：Applied biochemistry and biotechnology

authors： Alchihab M,Destain J,Aguedo M,Majad L,Ghalfi H,Wathelet JP,Thonart P

更新日期：2009-07-01 00:00:00

abstract：:Microbes are often subjected to oxidative stress in nature that badly affects their growth rate and viability. Although the response of microbes against oxidative stress has been characterized at the chemical, physiological, and molecular levels, the mechanism of gene-regulation network adaptations of bacteria in resp...

journal_title：Applied biochemistry and biotechnology

authors： Fei YY,Bhat JA,Gai JY,Zhao TJ

更新日期：2020-08-01 00:00:00

abstract：:In recent years, the concept of producing biodiesel from renewable lipid sources has regained international attention. In Brazil, a national program was launched in 2002 to evaluate the technical, economic, and environmental competitiveness of biodiesel in relation to the commercially available diesel oil. Several res...

journal_title：Applied biochemistry and biotechnology

authors： Ramos LP,Wilhelm HM

更新日期：2005-04-01 00:00:00

abstract：:Maltodextrins and hydrolysates of rice and corn flour of varying dextrose equivalents (DE) have been used as a carbon source for alpha-amylase enzyme production by Bacillus sp. TCRDC-25A. The rate and total enzyme production was higher in maltodextrin media than in cornstarch. The enzyme production increased with incr...

journal_title：Applied biochemistry and biotechnology

authors： Bajpai P,Bajpai PK

更新日期：1991-11-01 00:00:00

abstract：:Different treatments to improve the thermotolerance of fermenting yeasts for simultaneous ethanol saccharification and fermentation process of cellulosic materials have been examined. Yeasts of the genera Saccharomyces and Kluyveromyces were tested for growth and fermentation at progressively higher temperatures in th...

journal_title：Applied biochemistry and biotechnology

authors： Ballesteros I,Oliva JM,Ballesteros M,Carrasco J

更新日期：1993-04-01 00:00:00

abstract：:Bioethanol produced from lignocellulosic materials has been considered a sustainable alternative fuel. Such type of raw materials have a huge potential, but their hydrolysis into mono-sugars releases toxic compounds such as weak acids, which affect the microorganisms' physiology, inhibiting the growth and ethanol prod...

journal_title：Applied biochemistry and biotechnology

authors： Freitas C,Neves E,Reis A,Passarinho PC,da Silva TL

更新日期：2012-11-01 00:00:00

abstract：:A high cellobiohydrolase (CBH)-producing strain was isolated and identified as Penicillium purpurogenum KJS506 according to the morphology and comparison of internal transcribed spacer rDNA gene sequence. When rice straw and corn steep powder were used as carbon and nitrogen sources, respectively, a maximum CBH activi...

journal_title：Applied biochemistry and biotechnology

authors： Lee KM,Joo AR,Jeya M,Lee KM,Moon HJ,Lee JK

更新日期：2011-01-01 00:00:00

abstract：:Protein-protein interactions (PPIs) play pivotal roles in most of the biological processes. PPI dysfunctions are therefore associated with disease situations. Mutations often lead to PPI dysfunctions, but there are certain other types of mutations which do not cause any appreciable abnormalities. This second type of m...

journal_title：Applied biochemistry and biotechnology

authors： Ali A,Biswas R,Bhattacharjee S,Nath P,Pan S,Bagchi A

更新日期：2016-09-01 00:00:00

abstract：:Detection of minute quantities of target antigens in serum samples (consisting of a mixture of proteins/biomolecules) can be achieved by enhancement of the capture efficiencies of heterogeneous immunosensors. An important process parameter which affects the capture of target analytes in such immunosensors is the pH of...

journal_title：Applied biochemistry and biotechnology

authors： Rath D,Kumar S,Panda S

更新日期：2019-04-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:Fluorescence spectroscopy, UV-visible absorption spectroscopy, circular dichroism (CD) spectroscopy, viscometry, cyclic voltammetry (CV), and differential pulse voltammetry (DPV) were applied to investigate the competitive interaction of DNA with two aromatic α-aminobisphosphonates and neutral red dye (NR, intercalato...

journal_title：Applied biochemistry and biotechnology

authors： Gholivand MB,Peyman H,Gholivand K,Roshanfekr H,Taherpour AA,Yaghobi R

更新日期：2017-07-01 00:00:00

abstract：:This work describes the use of an ultrasound system for the enzymatic transesterification of oils using combi-lipases as biocatalyst. The reactions were carried out evaluating the individual use of waste oil and fresh soybean oil, and the immobilized lipases CALB, TLL, and RML were used as biocatalysts. It was perform...

journal_title：Applied biochemistry and biotechnology

authors： Poppe JK,Matte CR,Fernandez-Lafuente R,Rodrigues RC,Ayub MAZ

更新日期：2018-11-01 00:00:00

abstract：:The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, ...

journal_title：Applied biochemistry and biotechnology

authors： Lee S,Lee S,Yoon YJ,Lee J

更新日期：2013-01-01 00:00:00

abstract：:Feather waste, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Feather-degrading bacteria were isolated from a Brazilian poultry industrial waste. Among these isolates, a strain identified as kr2 was th...

journal_title：Applied biochemistry and biotechnology

authors： Sangali S,Brandelli A

更新日期：2000-04-01 00:00:00

abstract：:Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

journal_title：Applied biochemistry and biotechnology

authors： Liu K,Chua H,Lo WH,Lawford H,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Atomic force microscopy was used to study the geometric structure of collagen fibrils and molecules of rat calcanean tendon tissues. The authors found that the diameter of the fibrils ranged from 124 to 170 nm, and their geometric form suggested a helical winding with spectral period from 59.4 to 61.7 nm, close to the...

journal_title：Applied biochemistry and biotechnology

authors： Baranauskas V,Vidal BC,Parizotto NA

更新日期：1998-02-01 00:00:00

abstract：:During the course of fermentation, online measuring procedures able to estimate the performance of the current operation are highly desired. Unfortunately, the poor mechanistic understanding of most biologic systems hampers attempts at direct online evaluation of the bioprocess, which is further complicated by the lac...

journal_title：Applied biochemistry and biotechnology

authors： Bicciato S,Bagno A,Soldà M,Manfredini R,Di Bello C

更新日期：2002-07-01 00:00:00

abstract：:In rapidly growing developing countries, waste disposal is a major challenge. Current waste disposal methods (e.g., landfills and sewage treatment) incur costs and often are not employed; thus, wastes accumulate in the environment. To address this challenge, it is advantageous to create economic incentives to collect ...

journal_title：Applied biochemistry and biotechnology

authors： Lonkar S,Fu Z,Wales M,Holtzapple M

更新日期：2017-01-01 00:00:00

abstract：:Nostoc microscopicum an effective extracellular polymers (EPs) synthesizer among cyanobacteria was isolated, and its elementary morphological features were defined with the aid of light microscope and CLSM. Bioseparation of EPs from 44 days-aged culture gave 0.90 g/L of the dry powdered extract. Chemical quantificatio...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran L,Marappa N,Sethumadhavan K,Nooruddin T

更新日期：2020-05-01 00:00:00

abstract：:Pretreatment-induced structural alteration is critical in influencing the rate and extent of enzymatic saccharification of lignocellulosic biomass. The present work has investigated structural features of rice straw pretreated by hot-compressed water (HCW) from 140 to 240 °C for 10 or 30 min and enzymatic hydrolysis p...

journal_title：Applied biochemistry and biotechnology

authors： Yu G,Yano S,Inoue H,Inoue S,Wang J,Endo T

更新日期：2014-11-01 00:00:00

abstract：:Changes in protein glycosylation owing to changes in environmental conditions are not well understood. To better understand these relationships, methods to quantify controlling factors are needed. Because enzymes are translated from genes, the ability to quantify gene expression levels for glycosylation-related enzyme...

journal_title：Applied biochemistry and biotechnology

authors： Clark KJ,Harcum SW,Griffiths J,Bailey KM

更新日期：2005-06-01 00:00:00

abstract：:Large-scale amine-based CO(2) capture will generate waste containing large amounts of ammonia, in addition to contaminants such as the actual amine as well as degradation products thereof. Monoethanolamine (MEA) has been a dominant amine applied so far in this context. This study reveals how biological N removal can b...

journal_title：Applied biochemistry and biotechnology

authors： Hauser I,Colaço AB,Skjæran JA,Einbu A,Ostgaard K,Svendsen HF,Cervantes FJ

更新日期：2013-02-01 00:00:00

abstract：:Events from the past growth conditions influence the course of physiology, and it gets reflected in the present cell behaviour. During this process, cells acquire a metabolic option which carries signature of the past, and it dictates the performance in the present situation. Study uses Escherichia coli as a sample or...

journal_title：Applied biochemistry and biotechnology

authors： Puranik S,Purohit HJ

更新日期：2014-11-01 00:00:00

abstract：:The emergence of nalidixic acid-resistant strains of Salmonella typhimurium remains to be a major public health problem. In particular, the substitution of Asn in place of Asp at the 87 loci in the GyrA of S. typhimurium was experimentally stated for nalidixic acid resistance. However, the data on the possible mechani...

journal_title：Applied biochemistry and biotechnology

authors： Preethi B,Shanthi V,Ramanathan K

更新日期：2015-09-01 00:00:00

abstract：:Commercial conversion of lignocellulosic biomass to fermentable sugars requires inexpensive bulk production of biologically active cellulase enzymes, which might be achieved through direct production of these enzymes within the biomass crops. Transgenic corn plants containing the catalytic domain of Acidothermus cellu...

journal_title：Applied biochemistry and biotechnology

authors： Ransom C,Balan V,Biswas G,Dale B,Crockett E,Sticklen M

更新日期：2007-04-01 00:00:00

abstract：:Numerous microcapsule systems have been developed for a wide range of applications, including the sustained release of drugs, cell transplantation for therapy, cell immobilization, and other biotechnological applications. Despite the fact that microcapsule membrane is a dominant factor governing overall microcapsule p...

journal_title：Applied biochemistry and biotechnology

authors： Chen H,Ouyang W,Lawuyi B,Lim T,Prakash S

更新日期：2006-09-01 00:00:00

abstract：:To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes of FM-MMC-mc were made: one with the drug as the core and zinc ...

journal_title：Applied biochemistry and biotechnology

authors： Kato T,Nemoto R,Mori H,Abe R,Unno K,Goto A,Murota H,Harada M,Homma M

更新日期：1984-01-01 00:00:00

abstract：:Ornithine carbamoyltransferase folding/unfolding is a complex and not completely understood process. Our experimental results suggest that ornithine carbamoyltransferase interacts in a completely different way with urea and guanidine hydrochloride. In fact, we noticed that, increasing concentration from 0.0 to 8.0 M o...

journal_title：Applied biochemistry and biotechnology

authors： Barreca D,Laganà G,Ficarra S,Tellone E,Leuzzi U,Galtieri A,Bellocco E

更新日期：2014-01-01 00:00:00