Rapid screening and isolation of a fungus for sitosterol to androstenedione biotransformation.

abstract：:Biomass degradation compounds significantly inhibit biochemical conversion of biomass prehydrolysates to biofuels and chemicals, such as lactic acid. To characterize the structure-activity relationship of carbonyl inhibition on lactic acid fermentation, we examined effects of eight carbonyl compounds (furfural, 5-hydr...

journal_title：Applied biochemistry and biotechnology

authors： Li J,Zhu C,Tu M,Han P,Wu Y

更新日期：2015-04-01 00:00:00

abstract：:Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were highly active against subti...

journal_title：Applied biochemistry and biotechnology

authors： Kodrík D,Kludkiewicz B,Navrátil O,Skoková Habuštová O,Horáčková V,Svobodová Z,Vinokurov KS,Sehnal F

更新日期：2013-09-01 00:00:00

abstract：:The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilize...

journal_title：Applied biochemistry and biotechnology

authors： Jones F,Bailey R,Wilson S,Hiestand J

更新日期：2007-04-01 00:00:00

abstract：:The biosurfactant-producing Pseudomonas aeruginosa A11, with plant-growth-promoting (PGP) and multi-metal-resistant (MMR) features was isolated from the rhizosphere of a wild plant Parthenium hysterophorus. The strain A11 was able to utilize glycerol as a carbon source and produce 4,436.9 mg/L of biosurfactant after 1...

journal_title：Applied biochemistry and biotechnology

authors： Singh AK,Cameotra SS

更新日期：2013-07-01 00:00:00

abstract：:Hydrogels are attractive biomaterials for three-dimensional cell culture and tissue engineering applications. The preparation of hydrogels using alginate and gelatin provides cross-linked hydrophilic polymers that can swell but do not dissolve in water. In this work, we first reinforced pure alginate by using polyoxye...

journal_title：Applied biochemistry and biotechnology

authors： Aroguz AZ,Baysal K,Adiguzel Z,Baysal BM

更新日期：2014-05-01 00:00:00

abstract：:Biological control of soil-borne plant pathogens is a potential alternative to the use of chemical pesticides, which have already been proved to be harmful to the environment. Several strains of the fungus Trichoderma have been isolated and found to be effective biocontrol agents of various soil-borne plant pathogenic...

journal_title：Applied biochemistry and biotechnology

authors： Chet I,Inbar J

更新日期：1994-07-01 00:00:00

abstract：:Recently, poly(3-hydroxybutyrate) (PHB) has been found in a few thermophilic strains where several advantages can be gained from running fermentation at high temperatures. Caldimonas manganoxidans, a thermophilic gram-negative bacterium, was investigated for the feasibility as a PHB-producing strain. It is suggested t...

journal_title：Applied biochemistry and biotechnology

authors： Hsiao LJ,Lin JH,Sankatumvong P,Wu TM,Li SY

更新日期：2016-11-01 00:00:00

abstract：:The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration...

journal_title：Applied biochemistry and biotechnology

authors： Zhou Y,Domínguez JM,Cao N,Du J,Tsao GT

更新日期：1999-04-01 00:00:00

abstract：:To achieve targeted distribution of anticancer drugs with sustained activity, ferromagnetic ethylcellulose microcapsules containing an anticancer drug, mitomycin C (FM-MMC-mc), were prepared by a method based on phase separation principles. Two prototypes of FM-MMC-mc were made: one with the drug as the core and zinc ...

journal_title：Applied biochemistry and biotechnology

authors： Kato T,Nemoto R,Mori H,Abe R,Unno K,Goto A,Murota H,Harada M,Homma M

更新日期：1984-01-01 00:00:00

abstract：:The stabilization of olive recombinant hydroperoxide lyases (rHPLs) was investigated using selected chemical additives. Two rHPLs were studied: HPL full-length and HPL with its chloroplast transit peptide deleted (matured HPL). Both olive rHPLs are relatively stable at 4 °C, and enzyme activity can be preserved (about...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Vincenti S,Mariani M,Brunini-Bronzini de Caraffa V,Gambotti C,Desjobert JM,Muselli A,Costa J,Tomi F,Berti L,Maury J

更新日期：2017-07-01 00:00:00

abstract：:This study investigated the effects of extremely low frequency (ELF) magnetic field with/without iron(III) chloride (FeCl3) on bacterial growth and morphology. The ELF exposures were carried out using a pair of Helmholtz coil-based ELF exposure system which was designed to generate 50 Hz sinusoidal magnetic field. The...

journal_title：Applied biochemistry and biotechnology

authors： Esmekaya MA,Acar SI,Kıran F,Canseven AG,Osmanagaoglu O,Seyhan N

更新日期：2013-04-01 00:00:00

abstract：:Cofermentation of glucose, xylose, and arabinose is critical for complete bioconversion of lignocellulosic biomass, such as agricultural residues and herbaceous energy crops, to ethanol. We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, a...

journal_title：Applied biochemistry and biotechnology

authors： Mohagheghi A,Evans K,Chou YC,Zhang M

更新日期：2002-04-01 00:00:00

abstract：:The semi-continuous anaerobic digestion of food waste was investigated in 1-L and 20-L continuously stirred tank reactors (CSTRs), to identify the optimum operation condition and the methane production of the semi-continuous anaerobic process. Results from a 1-L digester indicated that the optimum organic loading rate...

journal_title：Applied biochemistry and biotechnology

authors： Zhang C,Su H,Wang Z,Tan T,Qin P

更新日期：2015-04-01 00:00:00

abstract：:Termitomyces fungi associated with fungus-growing termites are the edible mushrooms and can produce useful chemicals, enzymes, and volatile organic compounds (VOCs) that have both fuel and biological potentials. To this purpose, we examined the Termitomyces mycelial growth performance on various substrates, clarified ...

journal_title：Applied biochemistry and biotechnology

authors： Yang G,Ahmad F,Liang S,Fouad H,Guo M,Gaal HA,Mo J

更新日期：2020-12-01 00:00:00

abstract：:The effects of amino acid composition and peptide molecular mass on ACE-inhibitory and antioxidant activities of protein fragments obtained from tomato waste fermented using Bacillus subtilis were evaluated. The addition of B. subtilis increased the relative amounts of aromatic and positively-charged amino acids which...

journal_title：Applied biochemistry and biotechnology

authors： Moayedi A,Mora L,Aristoy MC,Hashemi M,Safari M,Toldrá F

更新日期：2017-01-01 00:00:00

abstract：:Genetic polymorphism of the cytochrome P450 (CYP) genes particularly affects CYP2D6 and CYP2C19 to a functionally relevant extent, and it is therefore crucial to elucidate the enzyme kinetic and molecular basis for altered catalytic activity of these allelic variants. This study explored the expression and function of...

journal_title：Applied biochemistry and biotechnology

authors： Dong AN,Pan Y,Palanisamy UD,Yiap BC,Ahemad N,Ong CE

更新日期：2018-09-01 00:00:00

abstract：:A new medical application of an immobilized microbial enzyme is described. Extracorporeal devices require systemic heparin administration to prevent thrombus formation; however, the use of heparin often leads to serious hemorrhagic complications. Heparinase isolated from Flavobacterium has been immobilized and used in...

journal_title：Applied biochemistry and biotechnology

authors： Linhardt RJ,Cooney CL,Tapper D,Zannetos CA,Larsen AK,Langer R

更新日期：1984-02-01 00:00:00

abstract：:The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate w...

journal_title：Applied biochemistry and biotechnology

authors： Kumar K,Bhargava P,Roy U

更新日期：2011-08-01 00:00:00

abstract：:The potential of dilute-acid prehydrolysis as a pretreatment method for sugarcane bagasse, rice hulls, peanut shells, and cassava stalks was investigated. The prehydrolysis was performed at 122 degrees C during 20, 40, or 60 min using 2% H(2)SO(4) at a solid-to-liquid ratio of 1:10. Sugar formation increased with incr...

journal_title：Applied biochemistry and biotechnology

authors： Martin C,Alriksson B,Sjöde A,Nilvebrant NO,Jönsson LJ

更新日期：2007-04-01 00:00:00

abstract：:The articular cartilage is an avascular and aneural tissue and its injuries result mostly in osteoarthritic changes and formation of fibrous tissue. Efforts of scientists worldwide are focused on restoration of cartilage with increase in life quality of patients. Novel polymeric polyhydroxybutyrate/chitosan (PCH) poro...

journal_title：Applied biochemistry and biotechnology

authors： Giretova M,Medvecky L,Petrovova E,Cizkova D,Danko J,Mudronova D,Slovinska L,Bures R

更新日期：2019-10-01 00:00:00

abstract：:Prostate apoptosis response-4 (Par-4), an anticancer protein that interacts with cell surface receptor GRP78, can selectively suppress proliferation and induce apoptosis of cancer cells. The core domain of Par-4 (aa 137-195), designated as SAC, is sufficient to inhibit tumor growth and metastasis without harming norma...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Sun A,Dong Y,Wei D

更新日期：2018-04-01 00:00:00

abstract：:Ovary-specific acidic protein (OSAP) is an important reproduction-related gene. In this study, we cloned the full-length cDNA sequence of porcine OSAP gene through the rapid amplification of cDNA ends method. The porcine OSAP gene encodes a protein of 238 amino acids which shares high homology with the OSAP of five sp...

journal_title：Applied biochemistry and biotechnology

authors： Lei Y,Yonggang L

更新日期：2012-09-01 00:00:00

abstract：:The ethanologenic bacteria Escherichia coli strains KO11 and LYO1, and Klebsiella oxytoca strain P2, were investigated for their ability to metabolize furfural. Using high performance liquid chromatography and 13C-nuclear magnetic resonance spectroscopy, furfural was found to be completely biotransformed into furfuryl...

journal_title：Applied biochemistry and biotechnology

authors： Gutiérrez T,Buszko ML,Ingram LO,Preston JF

更新日期：2002-04-01 00:00:00

abstract：:The effect of surfactant on enzymatic digestibility was investigated during the pretreatment stage. Newspaper was pretreated with an ammonia-hydrogen peroxide mixture on a shaking bath at 40 degrees C and 130 strokes/min for 3 h. Two kinds of nonionic surfactants, NP series and Tween series, were utilized. The effect ...

journal_title：Applied biochemistry and biotechnology

authors： Kim SB,Chun JW

更新日期：2004-04-01 00:00:00

abstract：:Streptomyces badius DB-1 produces α-amylase extracellularly, and its production was enhanced 5.1-fold (from 9.47 ± 0.51 to 48.23 ± 1.45 U mL-1) due to optimization by one-variable-at-a-time and statistical approaches. Soluble starch emerged as the most influential factor that strongly affected enzyme production. The p...

journal_title：Applied biochemistry and biotechnology

authors： Shivlata L,Satyanarayana T

更新日期：2017-04-01 00:00:00

abstract：:Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Four...

journal_title：Applied biochemistry and biotechnology

authors： Singh S,Singh AN,Verma A,Dubey VK

更新日期：2013-12-01 00:00:00

abstract：:The goal of this study was to increase the production of long-chain fatty acids and to change the composition of fatty acids through the overexpression of genes involved in the fatty acid synthase (FAS) pathway and utilizing characteristics of a specific gene, namely, fabF. The four genes, fabB, fabG, fabZ, and fabI, ...

journal_title：Applied biochemistry and biotechnology

authors： Lee S,Lee S,Yoon YJ,Lee J

更新日期：2013-01-01 00:00:00

abstract：:A real-time PCR quantification method for indigenous hydrocarbon-degrading bacteria (HDB) carrying the alkB gene in the soil environment was developed to investigate their distribution in soil. The detection limit of indigenous HDB by the method was 1 × 10(6) cells/g-soil. The indigenous HDB were widely distributed th...

journal_title：Applied biochemistry and biotechnology

authors： Fukuhara Y,Horii S,Matsuno T,Matsumiya Y,Mukai M,Kubo M

更新日期：2013-05-01 00:00:00

abstract：:Over the last two decades many strategies have been planned to design specific drugs for rare diseases to target their action at the DNA level. Advancements in our understanding of the interactions of small nonpeptide molecules with DNA have opened the doors for "rational" drug design. Special methods have now been de...

journal_title：Applied biochemistry and biotechnology

authors： Prabhakar P,Kayastha AM

更新日期：1994-04-01 00:00:00

abstract：:Aspergillus niger CTBU isolated from local decayed bamboo shoot residue was employed to solid-state fermentation (SSF) of bamboo shoot residue to elevate the content of phytosterols. Strain acclimatization was carried out under the fermentation condition using bamboo shoot as substrate for fermentation performance imp...

journal_title：Applied biochemistry and biotechnology

authors： Zheng XX,Chen RS,Shen Y,Yin ZY

更新日期：2014-04-01 00:00:00