In vitro refolding of triosephosphate isomerase from L. donovani.


:The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate was solubilized in 6 M guanidine hydrochloride and purified by Ni-NTA chromatography. In the present study, the effect of bovine serum albumin on the reactivation of TIM was investigated. Furthermore, 8-anilino-1-naphthalene sulfonic acid was used to detect the structural changes induced by bovine serum albumin (BSA). Here, we conclude that BSA assists in the refolding and regain of LdTIM enzyme activity by providing framework for structure formation. This study indicates that numerous protein-protein contacts are constantly occurring inside the cell that leads to the formation of native protein.


Kumar K,Bhargava P,Roy U




Has Abstract


2011-08-01 00:00:00












  • Enhanced Bioethanol Fermentation by Sonication Using Three Yeasts Species and Kariba Weed (Salvinia molesta) as Biomass Collected from Lake Victoria, Uganda.

    abstract::Kariba weed (Salvinia molesta) was used as biomass feedstock for ethanol production by separate hydrolysis and fermentation (SHF). Monosaccharides from Kariba weed hydrolysate were produced using thermal acid hydrolysis, sonication, and enzymatic saccharification. The optimal conditions for thermal acid hydrolysis of ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kityo MK,Sunwoo I,Kim SH,Park YR,Jeong GT,Kim SK

    更新日期:2020-09-01 00:00:00

  • Biodegradation of formaldehyde by a formaldehyde-resistant bacterium isolated from seawater.

    abstract::A formaldehyde-tolerant bacterium designated as a DM-2 strain was used to biodegrade formaldehyde. The cells, precultivated in the presence of 400 ppm of formaldehyde, were able to degrade formaldehyde in a minimal medium supplemented with up to 400 ppm of formaldehyde in the presence of 3% NaCl. The rate of formaldeh...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Yamazaki T,Tsugawa W,Sode K

    更新日期:2001-04-01 00:00:00

  • Expression and Characterization of a Novel Antifungal Exo-β-1,3-glucanase from Chaetomium cupreum.

    abstract::A novel β-1,3-glucanase gene, designated Ccglu17A, was cloned from the biological control fungus Chaetomium cupreum Ame. Its 1626-bp open reading frame encoded 541 amino acids. The corresponding amino acid sequence showed highest identity (67 %) with a glycoside hydrolase family 17 β-1,3-glucanase from Chaetomium glob...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jiang C,Song J,Cong H,Zhang J,Yang Q

    更新日期:2017-05-01 00:00:00

  • Saccharification of marine microalgae using marine bacteria for ethanol production.

    abstract::The saccharification of marine microalgae using amylase from marine bacteria in saline conditions was investigated. An amylase-producing bacterium, Pseudoalterimonas undina NKMB 0074 was isolated and identified. The green microalga NKG 120701 was determined to have the highest concentration of intracellular carbohydra...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Matsumoto M,Yokouchi H,Suzuki N,Ohata H,Matsunaga T

    更新日期:2003-04-01 00:00:00

  • Thermal effects on hydrothermal biomass liquefaction.

    abstract::Batch pressure vessels commonly used for hydrothermal liquefaction have typical heating times in the range of 30 to 60 min. Thermodynamically, the complex set of reactions are path dependent, so that the heating rate can possibly affect yields and the composition of the resultant liquid products. It is postulated that...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang B,von Keitz M,Valentas K

    更新日期:2008-03-01 00:00:00

  • Optimization of the simultaneous saccharification and fermentation process using thermotolerant yeasts.

    abstract::Different treatments to improve the thermotolerance of fermenting yeasts for simultaneous ethanol saccharification and fermentation process of cellulosic materials have been examined. Yeasts of the genera Saccharomyces and Kluyveromyces were tested for growth and fermentation at progressively higher temperatures in th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ballesteros I,Oliva JM,Ballesteros M,Carrasco J

    更新日期:1993-04-01 00:00:00

  • Foot and mouth disease virus concentration and purification by affinity chromatography.

    abstract::Foot and mouth disease virus, (FMDV) from a crude cell lysate was purified in a single step by affinity chromatography with heparin as a ligand. The virus eluted from an Heparin-Ultrogel A4R column at 1M sodium chloride in 10 mM sodium phosphate buffer, pH 7.0, while most cell protein and albumin did so at lower conce...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Navarro del Cañizo AA,Mazza M,Bellinzoni R,Cascone O

    更新日期:1996-12-01 00:00:00

  • Improved synthesis of (S)-1-phenyl-2-propanol in high concentration with coupled whole cells of Rhodococcus erythropolis and Bacillus subtilis on preparative scale.

    abstract::Bioreduction of 1-phenyl-2-propanone to prepare (S)-1-phenyl-2-propanol, a useful pharmaceutical intermediate, was performed with growing cells of Rhodococcus erythropolis JX-021, giving 14 mM (1.9 g/L) product in 99% e.e. at 5 h in the catalysis of 15 mM substrate. The reduction stopped afterwards due to strong inhib...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jin JZ,Li H,Zhang J

    更新日期:2010-11-01 00:00:00

  • Characterization of a recombinant flocculent Saccharomyces cerevisiae strain that co-ferments glucose and xylose: II. influence of pH and acetic acid on ethanol production.

    abstract::The inhibitory effects of pH and acetic acid on the co-fermentation of glucose and xylose in complex medium by recombinant flocculent Saccharomyces cerevisiae MA-R4 were evaluated. In the absence of acetic acid, the fermentation performance of strain MA-R4 was similar between pH 4.0-6.0, but was negatively affected at...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Matsushika A,Sawayama S

    更新日期:2012-12-01 00:00:00

  • Modelling growth and bacteriocin production by Pediococcus acidilactici PA003 as a function of temperature and pH value.

    abstract::To investigate the effect of pH and temperature on the cell growth and bacteriocin production of Pediococcus acidilactici PA003, a lactic acid bacterium isolated from traditionally fermented cabbage, the kinetic behaviour of P. acidilactici PA003 was simulated in vitro during laboratory fermentations by making use of ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang J,Zhang Y,Liu SN,Han Y,Zhou ZJ

    更新日期:2012-03-01 00:00:00

  • Biological Pretreatment of Chicken Feather and Biogas Production from Total Broth.

    abstract::Chicken feathers are available in large quantities around the world causing environmental challenges. The feathers are composed of keratin that is a recalcitrant protein and is hard to degrade. In this work, chicken feathers were aerobically pretreated for 2-8 days at total solid concentrations of 5, 10, and 20 % by B...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Patinvoh RJ,Feuk-Lagerstedt E,Lundin M,Sárvári Horváth I,Taherzadeh MJ

    更新日期:2016-12-01 00:00:00

  • Influence of blocking of 2,3-butanediol pathway on glycerol metabolism for 1,3-propanediol production by Klebsiella oxytoca.

    abstract::Glycerol metabolism is a typical biological oxidoreductive reaction. 1,3-Propanediol (1,3-PD) is the final product of the reductive branch, while acetate, succinate, lactate, 2,3-butanediol (2,3-BD), and ethanol were produced in the oxidative branch. 2,3-BD, which has similar properties of high boiling point and water...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhang G,Yang G,Wang X,Guo Q,Li Y,Li J

    更新日期:2012-09-01 00:00:00

  • Leucas aspera Nanomedicine Shows Superior Toxicity and Cell Migration Retarded in Prostate Cancer Cells.

    abstract::Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Mohan A,Nair SV,Lakshmanan VK

    更新日期:2017-04-01 00:00:00

  • Sequential anaerobic/aerobic treatment of dye-containing wastewaters: colour and COD removals, and ecotoxicity tests.

    abstract::Colour and COD removals of the azo dyes Congo Red (CR) and Reactive Black 5 (RB5) were individually evaluated in a sequential anaerobic/aerobic treatment system. Additionally, dye toxicity was assessed by using acute ecotoxicity tests with Daphnia magna as the indicator-organism. The anaerobic reactor was operated at ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Silva ME,Firmino PI,Sousa MR,Santos AB

    更新日期:2012-02-01 00:00:00

  • A magnetizable solid phase for enzyme extraction.

    abstract::A method for the convenient and reliable preparation of magnetizable agarose beads containing iron particles is described. The beads were treated with the triazine dye, Reactive Red 120, and the matrix was examined for the ability to extract proteins from crude preparations using lactate dehydrogenase from porcine mus...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ennis MP,Wisdom GB

    更新日期:1991-08-01 00:00:00

  • Induction of apoptosis by ribosome inactivating proteins: importance of N-glycosidase activity.

    abstract::Apoptotic cell death is a fundamental process in the development and physiological homeostasis of multicellular organisms. It is associated with control of cell numbers in tissues and organs during development, with cell turnover, and with response to infection. Molecules that trigger this process in continuously prol...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审


    authors: Das MK,Sharma RS,Mishra V

    更新日期:2012-03-01 00:00:00

  • A new method for microcapsule characterization: use of fluorogenic genipin to characterize polymeric microcapsule membranes.

    abstract::Numerous microcapsule systems have been developed for a wide range of applications, including the sustained release of drugs, cell transplantation for therapy, cell immobilization, and other biotechnological applications. Despite the fact that microcapsule membrane is a dominant factor governing overall microcapsule p...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Chen H,Ouyang W,Lawuyi B,Lim T,Prakash S

    更新日期:2006-09-01 00:00:00

  • Electrokinetic-Enhanced Remediation of Phenanthrene-Contaminated Soil Combined with Sphingomonas sp. GY2B and Biosurfactant.

    abstract::Electrokinetic-microbial remediation (EMR) has emerged as a promising option for the removal of polycyclic aromatic hydrocarbons (PAHs) from contaminated soils. The aim of this study was to enhance degradation of phenanthrene (Phe)-contaminated soils using EMR combined with biosurfactants. The electrokinetic (EK) reme...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审


    authors: Lin W,Guo C,Zhang H,Liang X,Wei Y,Lu G,Dang Z

    更新日期:2016-04-01 00:00:00

  • Isolation and characterization of dimethyl sulfide (DMS)-degrading bacteria from soil and biofilter treating waste gas containing DMS from the laboratory and pulp and paper industry.

    abstract::Dimethyl sulfide (DMS) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. DMS has environmental health implications; therefore, it is necessary to treat the waste gas containing DMS prior to discharge into the environment. A bench-scale biofilter was operated in the...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Giri BS,Juwarkar AA,Satpute DB,Mudliar SN,Pandey RA

    更新日期:2012-07-01 00:00:00

  • Intracellular fate of hydrocarbons: possible existence of specific compartments for their biodegradation.

    abstract::In previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the YR-1 strain of Mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sAO) activity. In the present work enzym...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Silva-Jiménez H,Zazueta-Sandoval R

    更新日期:2005-04-01 00:00:00

  • Fed-batch anaerobic valorization of slaughterhouse by-products with mesophilic microbial consortia without methane production.

    abstract::This work aimed at setting up a fully instrumented, laboratory-scale bioreactor enabling anaerobic valorization of solid substrates through hydrogen and/or volatile fatty acid (VFA) production using mixed microbial populations (consortia). The substrate used was made of meat-based wastes, especially from slaughterhous...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pessiot J,Nouaille R,Jobard M,Singhania RR,Bournilhas A,Christophe G,Fontanille P,Peyret P,Fonty G,Larroche C

    更新日期:2012-07-01 00:00:00

  • Effects of temperature and substrate concentration on lipid production by Chlorella vulgaris from enzymatic hydrolysates of lipid-extracted microalgal biomass residues (LMBRs).

    abstract::The enzymatic hydrolysates of the lipid-extracted microalgal biomass residues (LMBRs) from biodiesel production were evaluated as nutritional sources for the mixotrophic growth of Chlorella vulgaris and lipid production at different temperature levels and substrate concentrations. Both parameters had a significant eff...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ma X,Zheng H,Huang H,Liu Y,Ruan R

    更新日期:2014-10-01 00:00:00

  • Effect of agitation rate on ethanol production from sugar maple hemicellulosic hydrolysate by Pichia stipitis.

    abstract::Concentrated dilute acid hydrolysate was obtained from hot water extracts of Acer saccharum (sugar maple) and was fermented to ethanol by Pichia stipitis in a 1.3-L-benchtop bioreactor. The conditions under which the highest ethanol yield was achieved were when the air flow rate was set to 100 cm(3) and the agitation ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Shupe AM,Liu S

    更新日期:2012-09-01 00:00:00

  • Characterization of biosorption process of acid orange 7 on waste brewery's yeast.

    abstract::The use of cheap, high-efficiency, and ecofriendly adsorbent has been studied as an alternative way for the removal of dyes from wastewater. This paper investigated the use of waste brewery's yeast for the removal of acid orange 7 from aqueous solution. The optimum removal of acid orange 7 was found to be 3.561 mg/g a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Wu Y,Hu Y,Xie Z,Feng S,Li B,Mi X

    更新日期:2011-04-01 00:00:00

  • Effect of VIB Gene on Cellulase Production of Trichoderma orientalis EU7-22.

    abstract::The cellulolytic enzymes from filamentous fungi are widely used for production of biofuels. Molecular biological engineering of fungal strains has been applied to improve the cellulase production in the recent years. The VIB gene affects production of cellulase and known as a new transcription factor. Based on a fast-...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Han J,Xue Y,Li M,Li Y,Liu J,Gan L,Long M

    更新日期:2020-08-01 00:00:00

  • Growth Parameters, Photosynthetic Performance, and Biochemical Characterization of Newly Isolated Green Microalgae in Response to Culture Condition Variations.

    abstract::This work aimed to characterize two native microalgal strains newly isolated from South Mediterranean areas and identified as Chlorella sorokiniana ES3 and Neochloris sp. AM2. The growth properties and biochemical composition of these microalgae were evaluated in different culture media (Algal, BG-11, f/2, and Conway)...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jazzar S,Berrejeb N,Messaoud C,Marzouki MN,Smaali I

    更新日期:2016-08-01 00:00:00

  • Optimization of fermentation parameters to enhance the production of ethanol from palmyra jaggery using Saccharomyces cerevisiae in a batch fermentor.

    abstract::Application of statistical experimental designs for optimization of fermentation parameters to enhance ethanol production, which is an economical and renewable energy source using Saccharomyces cerevisiae NCIM 3090 from palmyra jaggery, was studied in a batch fermentor. Using Plackett-Burman design, impeller speed, co...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Bandaru VV,Bandaru SR,Somalanka SR,Mendu DR,Imandi SB,Bejawada SR,Medicherla NR,Devarajan T,Karothi J,Chityala A

    更新日期:2007-12-01 00:00:00

  • In Vivo Evaluation of the Toxic Effect of Ethyl Acetate Extracts of Marine Antibiotic Resistance Pseudomonas Species Derived from the Red Sea.

    abstract::Eighty-nine cultured Pseudomonas species isolated from the sediment and water samples collected from five industrial Red Sea regions that have been affected by petroleum and industry. Genotypic (exoT, exoS, exoU, exoY, lasA, lasB, rhlA, rhlB, Pf1, PAGI-1, -2, and -3) and phenotypic (DNase, elastase, lipase, protease, ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: El-Gendy MMAA,Al-Zahrani HAA,Abozinadah NY,El-Bondkly AMA

    更新日期:2018-01-01 00:00:00

  • Development and application of an integrated system for monitoring ethanol content of fuels.

    abstract::An automated flow injection analysis (FIA) system for quantifying ethanol was developed using alcohol oxidase, horseradish peroxidase, 4-amino-phenazone, and phenol. A colorimetric detection method was developed using two different methods of analysis, with free and immobilized enzymes. The system with free enzymes pe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Alhadeff EM,Salgado AM,Pereira N Jr,Valdman B

    更新日期:2004-04-01 00:00:00

  • Improvement in thermal stability and substrate binding of pig kidney D-amino acid oxidase by chemical modification.

    abstract::Chemical modification was evaluated to stabilize pig kidney D-amino acid oxidase (pkDAAO), which is required for analytical determination of D-amino acids. Optimization of modification conditions was performed to obtain high recovery yield and stability, and chemical modification at 30 degrees C for 12 h with a highly...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Bakke M,Kajiyama N

    更新日期:2004-03-01 00:00:00