Abstract：
:The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate was solubilized in 6 M guanidine hydrochloride and purified by Ni-NTA chromatography. In the present study, the effect of bovine serum albumin on the reactivation of TIM was investigated. Furthermore, 8-anilino-1-naphthalene sulfonic acid was used to detect the structural changes induced by bovine serum albumin (BSA). Here, we conclude that BSA assists in the refolding and regain of LdTIM enzyme activity by providing framework for structure formation. This study indicates that numerous protein-protein contacts are constantly occurring inside the cell that leads to the formation of native protein.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Kumar K,Bhargava P,Roy Udoi
10.1007/s12010-011-9206-2subject
Has Abstractpub_date
2011-08-01 00:00:00pages
1207-14issue
7eissn
0273-2289issn
1559-0291journal_volume
164pub_type
杂志文章abstract：:The notch signaling pathway plays an important role in inhibiting cell differentiation and enhancing the repopulation capability of hematopoietic stem/progenitor cells. In this study, we developed rhDSL, a novel soluble form of Notch ligand Delta-like-1, which contains the DSL domain and the N-terminal sequence of the...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-009-8603-2
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abstract：:Recombinant tissue-plasminogen activator (r-tPA), expressed in Escherichia coli cells in an aggregated form, was solubilized with a strong chaotrope in the absence of any reducing agent. The solubilized molecule was reactivated by a procedure that was developed to mimic the physiological conditions optimal for the fun...
journal_title：Applied biochemistry and biotechnology
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abstract：:The three-dimensional distribution (x, y, and z) of ultrasound-induced microalgal cell disruption in a sonochemical reactor was predicted by solving the Helmholtz equation using a three-dimensional acoustic module in the COMSOL Multiphysics software. The simulated local ultrasound pressure at any given location (x, y,...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-015-1937-z
更新日期：2016-03-01 00:00:00
abstract：:The high cost of nicotinamide cofactors requires that they be regenerated in situ when used in preparative enzymatic synthesis. Numerous strategies have been tested for in situ regeneration of reduced and oxidized cofactors. Regeneration of reduced cofactors is relatively straightforward; regeneration of oxidized cofa...
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pub_type： 杂志文章,评审
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abstract：:Plant cell cultures provide a large potential for the production of secondary metabolites. Through the application of different physical and chemical cell stress factors, we investigated the production of the secondary metabolites in plant cell cultures. The effects of pulsed electric field (PEF) and ethephon on growt...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-010-9146-2
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doi：10.1007/s12010-014-0909-z
更新日期：2014-06-01 00:00:00
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abstract：:The corn wet milling process produces a 10% (w/w of the processed corn) byproduct called corn fiber, which is utilized worldwide as a low-value feedstock for cattle. The aim of this study was to find a higher value use of corn fiber. The main fractions of corn fiber are: 20% starch, 40% hemicellulose, 14% cellulose, a...
journal_title：Applied biochemistry and biotechnology
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doi：10.1385/ABAB:131:1:738
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更新日期：2009-07-01 00:00:00
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doi：10.1007/s12010-015-1771-3
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doi：10.1007/BF02920463
更新日期：1997-04-01 00:00:00
abstract：:In the production of ethanol from lignocellulosic biomass, the hydrolysis of the acetylated pentosans in hemicellulose during pretreatment produces acetic acid in the prehydrolysate. The National Renewable Energy Laboratory (NREL) is currently investigating a simultaneous saccharification and cofermentation (SSCF) pro...
journal_title：Applied biochemistry and biotechnology
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doi：10.1007/BF02920133
更新日期：1998-04-01 00:00:00
abstract：:Artemisinin is an important drug commonly used in the treatment of malaria as a combination therapy. It is primarily produced by a plant Artemisia annua, however, its supply from plant is significantly lower than its huge demand and therefore alternative in vitro production routes are sought. Hairy root cultivation co...
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pub_type： 杂志文章
doi：10.1007/s12010-014-1176-8
更新日期：2014-11-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s12010-016-2221-6
更新日期：2017-01-01 00:00:00
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doi：10.1007/s12010-013-0154-x
更新日期：2013-04-01 00:00:00
abstract：:Many lab-scale studies have been carried out regarding the effect of feed strategy on the performance of anaerobic sequencing batch reactors (ASBR); however, more detailed pilot-scale studies should be performed to assess the real applicability of this type of operation. Therefore, the objective of this work was to as...
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更新日期：1984-10-01 00:00:00
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abstract：:The unprecedented coronavirus SARS-CoV-2 outbreak at Wuhan, China, caused acute respiratory infection to humans. There is no precise vaccine/therapeutic agents available to combat the COVID-19 disease. Some repurposed drugs are saving the life of diseased, but the complete cure is relatively less. Several drug targets...
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pub_type： 杂志文章
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abstract：:Corn fiber is the fibrous by-product of wet-mill corn processing. It typically consists of about 20% starch, 14% cellulose, and 30% hemicellulose in the form of arabinoxylan. Crude corn fiber (CCF) was fractionated into de-starched corn fiber (DSCF), corn fiber with cellulose (CFC) enriched, and corn fiber arabinoxyla...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：
更新日期：2005-04-01 00:00:00
