In vitro refolding of triosephosphate isomerase from L. donovani.

Abstract:

:The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate was solubilized in 6 M guanidine hydrochloride and purified by Ni-NTA chromatography. In the present study, the effect of bovine serum albumin on the reactivation of TIM was investigated. Furthermore, 8-anilino-1-naphthalene sulfonic acid was used to detect the structural changes induced by bovine serum albumin (BSA). Here, we conclude that BSA assists in the refolding and regain of LdTIM enzyme activity by providing framework for structure formation. This study indicates that numerous protein-protein contacts are constantly occurring inside the cell that leads to the formation of native protein.

authors

Kumar K,Bhargava P,Roy U

doi

10.1007/s12010-011-9206-2

subject

Has Abstract

pub_date

2011-08-01 00:00:00

pages

1207-14

issue

7

eissn

0273-2289

issn

1559-0291

journal_volume

164

pub_type

杂志文章
  • Effect of ion and surfactant choice on the recovery of a histidine-tagged protein from tobacco extract using foam fractionation.

    abstract::Tobacco plants can be used for the production of proteins for pharmaceutical applications. One of the most difficult and expensive tasks associated with this technology is isolating the product of interest from the hundreds of other chemicals found in tobacco. We describe a new recovery strategy in which the protein o...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:119:1:79

    authors: Crofcheck C,Maiti I,Pattanaik S,Jay M

    更新日期:2004-10-01 00:00:00

  • Anticancer and DNA binding activities of platinum (IV) complexes; importance of leaving group departure rate.

    abstract::The two six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands with general formula [Pt(X)2Me2((t)bu2bpy)], where (t)bu2bpy = 4,4'-ditert-butyl-2,2'-bipyridine and X = Cl (C1) or Br (C2), serving as the leaving groups were synthesized for evaluation of their anticancer activities and DNA b...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0700-6

    authors: Pouryasin Z,Yousefi R,Nabavizadeh SM,Rashidi M,Hamidizadeh P,Alavianmehr MM,Moosavi-Movahedi AA

    更新日期:2014-03-01 00:00:00

  • Catalytic antibodies for complex reactions: hapten design and the importance of screening for catalysis in the generation of catalytic antibodies for the NDA/CN reaction.

    abstract::Success in generating catalytic antibodies as enzyme mimics lies in the strategic design of the transition-state analog (TSA) for the reaction of interest, and careful development of screening processes for the selection of antibodies that are catalysts. Typically, the choice of TSA structure is straightforward, and t...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:83:1-3:195

    authors: DeSilva BS,Orosz G,Egodage KL,Carlson RG,Schowen RL,Wilson GS

    更新日期:2000-01-01 00:00:00

  • Expression, purification, and antibacterial activity of bovine lactoferrampin-lactoferricin in Pichia pastoris.

    abstract::Bovine lactoferrampin (LFA) and bovine lactoferricin (LFC) are two antimicrobial peptides located in the N(1) domain of bovine lactoferrin. The bactericidal activity of the fused peptide LFA-LFC is stronger than that of either LFA or LFC. The high cost of peptide production from either native digestion or chemical syn...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9455-0

    authors: Tang XS,Tang ZR,Wang SP,Feng ZM,Zhou D,Li TJ,Yin YL

    更新日期:2012-02-01 00:00:00

  • New analytical method using coupled enzymes for determination of polyunsaturated fatty acid content in olive oil.

    abstract::A new, simple, and original method is described for specific measurement of polyunsaturated fatty acid content in olive oil. This analytical system uses coupled enzymes, lipase and lipoxygenase. The system consists of lipase-catalyzed hydrolysis of triacylglycerol and subsequent lipoxygenation of liberated polyunsatur...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-8935-y

    authors: Ben Rejeb I,Monser L,Gargouri M

    更新日期:2010-11-01 00:00:00

  • Dehydration of ethanol by facile synthesized glucose-based silica.

    abstract::Bioethanol is considered a potential liquid fuel that can be produced from biomass by fermentation and distillation. Although most of the water is removed by distillation, the purity of ethanol is limited to 95-96 % due to the formation of a low-boiling point, water-ethanol azeotrope. To improve the use of ethanol as ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-012-0076-z

    authors: Tang B,Bi W,Row KH

    更新日期:2013-02-01 00:00:00

  • Statistical optimization of alpha-amylase production with immobilized cells of Streptomyces erumpens MTCC 7317 in Luffa cylindrica L. sponge discs.

    abstract::The purpose of this investigation was to study the effect of Streptomyces erumpens cells immobilized in various matrices, i.e., agar-agar, polyacrylamide, and luffa (Luffa cylindrica L.) sponge for production of alpha-amylase. Luffa sponge was found to be 21% and 51% more effective in enzyme yield than agar-agar and p...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-008-8248-6

    authors: Kar S,Swain MR,Ray RC

    更新日期:2009-02-01 00:00:00

  • Biocatalytic removal of nickel and vanadium from petroporphyrins and asphaltenes.

    abstract::Asphaltenes from a crude oil rich in heavy metals (Castilla crude oil) were fractionated and partially characterized. Biocatalytic modifications of these fractionated asphaltenes by three different hemoproteins: chloro-peroxidase (CPO), cytochrome C peroxidase (Cit-C), and lignin peroxidase (LPO) were evaluated in bot...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920187

    authors: Mogolloń L,Rodríguez R,Larrota W,Ortiz C,Torres R

    更新日期:1998-04-01 00:00:00

  • L-Asparaginase as potent anti-leukemic agent and its significance of having reduced glutaminase side activity for better treatment of acute lymphoblastic leukaemia.

    abstract::Acute lymphoblastic leukaemia (ALL) is one of the leading types of malignant disorder seen in children. Viral infections, genetic factors and exposure to chemical carcinogens are some of the factors responsible for causing ALL. Treatment strategies followed for curing ALL include chemotherapy or radiation therapy, whe...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审

    doi:10.1007/s12010-012-9755-z

    authors: Ramya LN,Doble M,Rekha VP,Pulicherla KK

    更新日期:2012-08-01 00:00:00

  • Functional Characterization of a Robust Marine Microbial Esterase and Its Utilization in the Stereo-Selective Preparation of Ethyl (S)-3-Hydroxybutyrate.

    abstract::One novel microbial esterase PHE21 was cloned from the genome of Pseudomonas oryzihabitans HUP022 identified from the deep sea of the Western Pacific. PHE21 was heterologously expressed and functionally characterized to be a robust esterase which behaved high resistance to various metal ions, organic solvents, surfact...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2161-1

    authors: Wang Y,Zhang Y,Hu Y

    更新日期:2016-11-01 00:00:00

  • The new application of biosorption properties of Enteromorpha prolifera.

    abstract::The main goal of this paper was to elaborate the possibility of industrial application of biosorption properties of Enteromorpha prolifera (production of mineral feed additives for livestock). In this study, biosorption process was used in the binding of chromium(III) ions from aqueous solution by the green macroalga....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8635-7

    authors: Michalak I,Chojnacka K

    更新日期:2010-03-01 00:00:00

  • Ultrafiltration of thin stillage from conventional and e-mill dry grind processes.

    abstract::We used ultrafiltration (UF) to evaluate membrane filtration characteristics of thin stillage and determine solids and nutrient compositions of filtered streams. To obtain thin stillage, corn was fermented using laboratory methods. UF experiments were conducted in batch mode under constant temperature and flow rate co...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-010-9114-x

    authors: Arora A,Dien BS,Belyea RL,Wang P,Singh V,Tumbleson ME,Rausch KD

    更新日期:2011-05-01 00:00:00

  • Modulatory Effect of Indoles on the Expression of miRNAs Regulating G1/S Cell Cycle Phase in Breast Cancer Cells.

    abstract::Indole-3-carbinol (I3C) is a naturally occurring glucosinolate found in Brassica vegetables that is usually converted in gastric acidic environment to the efficient metabolite 3,3'-diindolylmethane (DIM). Both indoles (I3C and DIM) are known chemopreventive agents for various cancers including breast cancer. This stud...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03378-8

    authors: El-Daly SM,Gamal-Eldeen AM,Gouhar SA,Abo-Elfadl MT,El-Saeed G

    更新日期:2020-12-01 00:00:00

  • The effect of hydrogen ion on the steady-state multiplicity of substrate-inhibited enzymatic reactions. II. Transient behavior.

    abstract::In this paper we concentrate our attention on the stability and transient behavior of the isothermal system (CSTR) with a substrate-inhibited enzyme reaction producing hydrogen ions. Our investigation covers the region of multiple steady states uncovered previously (1) (ordinary hysteresis and isola). We investigate t...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02780380

    authors: Elnashaie SS,Elrifaie MA,Ibrahim G,Badra G

    更新日期:1983-12-01 00:00:00

  • Production, partial characterization, and use of a red biochrome produced by Serratia sakuensis subsp. nov strain KRED for dyeing natural fibers.

    abstract::We have described a novel red biochrome, 514 Da in size, produced by solid-state cultivation of a bacterial isolate obtained from garden soil. The growth requirements of the isolate, the chemical characteristics of the biochrome produced, and the application of the biochrome in dying of silk, wool, and cotton fabrics ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9427-4

    authors: Vaidyanathan J,Bhathena-Langdana Z,Adivarekar RV,Nerurkar M

    更新日期:2012-01-01 00:00:00

  • Immobilized carboxypeptidase N. A potent bioreactor and specific adsorbent for peptides.

    abstract::Carboxypeptidase N-Sepharose was prepared by covalent immobilization of purified human plasma carboxypeptidase N. More than 98% of the carboxypeptidase N was immobilized; 42% of the applied activity can be detected on the support. The column has excellent capabilities to quantitatively remove carboxy-terminal basic am...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02921652

    authors: Wang W,Hendriks DF,Scharpé SL

    更新日期:1994-02-01 00:00:00

  • Selection of microalgae and cyanobacteria strains for bicarbonate-based integrated carbon capture and algae production system.

    abstract::Using microalgae to capture CO2 from flue gas is an ideal way to reduce CO2 emission, but this is challenged by the high cost of carbon capture and transportation. To address this problem, a bicarbonate-based integrated carbon capture and algae production system (BICCAPS) has been proposed, in which bicarbonate is use...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0515-5

    authors: Chi Z,Elloy F,Xie Y,Hu Y,Chen S

    更新日期:2014-01-01 00:00:00

  • Fusel oil as precursor for aroma generation by biotransformation using lipase: scientific note.

    abstract::The feasibility of using mixtures of C4 and C5 chain-length aliphatic alcohols from fusel oil as the bulk organic media for lipase-mediated synthesis of laurate esters was assessed. Reaction mixtures consisted of lauric acid, lipase, solvent (if added), and appropriate amount of fusel oil (previously dehydrated with i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:79:1-3:817

    authors: De Castro HF,Moriya RY,Oliveira PC,Soares CM

    更新日期:1999-04-01 00:00:00

  • The effects of selenium on polyunsaturated fatty acids of Diasporangium jonesianum.

    abstract::Fungi had become the main resource of polyunsaturated fatty acids, especially linoleic acid. The research studied the effects and mechanism of selenium on polyunsaturated fatty acids of Diasporangium jonesianum. The results showed that selenium could significantly increase the yields of linoleic acid. In contrast, the...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0477-7

    authors: Jia Y,Guan XY,Dai CC

    更新日期:2014-01-01 00:00:00

  • Improving fermentation performance of recombinant Zymomonas in acetic acid-containing media.

    abstract::In the production of ethanol from lignocellulosic biomass, the hydrolysis of the acetylated pentosans in hemicellulose during pretreatment produces acetic acid in the prehydrolysate. The National Renewable Energy Laboratory (NREL) is currently investigating a simultaneous saccharification and cofermentation (SSCF) pro...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920133

    authors: Lawford HG,Rousseau JD

    更新日期:1998-04-01 00:00:00

  • Pilot-scale bioremediation of PAH-contaminated soils.

    abstract::The Institute of Gas Technology (IGT) conducted a pilot-scale study at a former manufactured gas plant (MGP) site in New Jersey. The objective of the study was to determine the effectiveness of an innovative chemical/biological treatment process (MGP-REM process) to remediate soils contaminated with polynuclear aromat...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02920473

    authors: Pradhan SP,Paterek JR,Liu BY,Conrad JR,Srivastava VJ

    更新日期:1997-04-01 00:00:00

  • Expression and characterization of a new thermostable esterase from Clostridium thermocellum.

    abstract::The thermostable esterase from the thermophilic bacterium Clostridium thermocellum DSM 1313 was expressed in Escherichia coli and purified by Ni(2+) affinity chromatography. Its molecular weight was approximately 35 kDa according to 12 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Th...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1824-7

    authors: Zhang T,Chen H,Ni Z,Tian R,Jia J,Chen Z,Yang S

    更新日期:2015-12-01 00:00:00

  • Expression and Characterization of a Recombinant Laccase with Alkalistable and Thermostable Properties from Streptomyces griseorubens JSD-1.

    abstract::Streptomyces griseorubens JSD-1 is a novel actinomycete that could grow efficiently upon lignin, and the ligninolytic genes active in this biotransformation were expected to be crucial. To investigate the molecular mechanism of utilizing lignin, genome sequencing was carried out to obtain its draft genome, which was d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-015-1594-2

    authors: Feng H,Zhang D,Sun Y,Zhi Y,Mao L,Luo Y,Xu L,Wang L,Zhou P

    更新日期:2015-05-01 00:00:00

  • Enhancing the Performance of Microbial Fuel Cell by Using Chloroform Pre-treated Mixed Anaerobic Sludge to Control Methanogenesis in Anodic Chamber.

    abstract::Formation of methane in the anodic chamber of a microbial fuel cell (MFC) indicates an energy inefficiency in electricity generation as the energy required for electrogenesis gets redirected to methanogenesis. The hypothesis of this research is that inhibition of methanogenesis in the mixed anaerobic anodic inoculum i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03458-9

    authors: Tholia V,Neethu B,Bhowmick GD,Ghangrekar MM

    更新日期:2020-11-16 00:00:00

  • Purification and characterization of pepsin-solubilized collagen from skin of sea cucumber Holothuria parva.

    abstract::Pepsin-solubilized collagen (PSC) was extracted from the skin of sea cucumber Holothuria parva and was fractionally characterized. The PSC from H. parva skin consisted of three α1 chains (α1)3, in contrast to calf skin collagen type I with two α1 and one α2 chains (α1)2α2 with approximately 130 kDa each. The maximum t...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0823-4

    authors: Adibzadeh N,Aminzadeh S,Jamili S,Karkhane AA,Farrokhi N

    更新日期:2014-05-01 00:00:00

  • ABCC subfamily vacuolar transporters are involved in Pb (lead) detoxification in Saccharomyces cerevisiae.

    abstract::The present work has as objective to contribute for the elucidation of the mechanism associated with Pb detoxification, using the yeast Saccharomyces cerevisiae as a model organism. The deletion of GTT1 or GTT2 genes, coding for functional glutathione transferases (GST) enzymes in S. cerevisiae, caused an increased su...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1252-0

    authors: Sousa CA,Hanselaer S,Soares EV

    更新日期:2015-01-01 00:00:00

  • Fed-batch anaerobic valorization of slaughterhouse by-products with mesophilic microbial consortia without methane production.

    abstract::This work aimed at setting up a fully instrumented, laboratory-scale bioreactor enabling anaerobic valorization of solid substrates through hydrogen and/or volatile fatty acid (VFA) production using mixed microbial populations (consortia). The substrate used was made of meat-based wastes, especially from slaughterhous...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9516-4

    authors: Pessiot J,Nouaille R,Jobard M,Singhania RR,Bournilhas A,Christophe G,Fontanille P,Peyret P,Fonty G,Larroche C

    更新日期:2012-07-01 00:00:00

  • Cloning and Characterization of Cold-Adapted α-Amylase from Antarctic Arthrobacter agilis.

    abstract::In this study, the gene encoding an α-amylase from a psychrophilic Arthrobacter agilis PAMC 27388 strain was cloned into a pET-28a(+) vector and heterologously expressed in Escherichia coli BL21(DE3). The recombinant α-amylase with a molecular mass of about 80 kDa was purified by using Ni2+-NTA affinity chromatography...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2267-5

    authors: Kim SM,Park H,Choi JI

    更新日期:2017-03-01 00:00:00

  • Efficient production of a thermophilic 2-deoxyribose-5-phosphate aldolase in glucose-limited fed-batch cultivations of Escherichia coli by continuous lactose induction strategy.

    abstract::The production of a thermophilic 2-deoxyribose-5-phosphate aldolases (DERA) in Escherichia coli BL21 under continuous lactose induction strategy was investigated. The process was combined with the exponential feeding method, controlling the feeding rate to maintain the specific growth rate at 0.15 h(-1). The results i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9261-8

    authors: Pei XL,Wang QY,Li CL,Qiu XF,Xie KL,Huang LF,Wang AM,Zeng ZW,Xie T

    更新日期:2011-09-01 00:00:00

  • The study of ascorbate peroxidase, catalase and peroxidase during in vitro regeneration of Argyrolobium roseum.

    abstract::Here, we demonstrate the micropropagation protocol of Argyrolobium roseum (Camb.), an endangered herb exhibiting anti-diabetic and immune-suppressant properties, and antioxidant enzymes pattern is evaluated. Maximum callogenic response (60 %) was observed from leaf explant at 1.0 mg L(-1) 1-nephthalene acetic acid (NA...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0591-6

    authors: Habib D,Chaudhary MF,Zia M

    更新日期:2014-01-01 00:00:00