In vitro refolding of triosephosphate isomerase from L. donovani.


:The triosephosphate isomerase of Leishmania donovani (LdTIM) was expressed at high level in Escherichia coli. The TIM gene was cloned in expression vector pET-23(a) with C-terminal 6× His tag fused in frame, and expressed as a 27.6-kDa protein in E. coli as inclusion bodies. The recombinant LdTIM from E. coli lysate was solubilized in 6 M guanidine hydrochloride and purified by Ni-NTA chromatography. In the present study, the effect of bovine serum albumin on the reactivation of TIM was investigated. Furthermore, 8-anilino-1-naphthalene sulfonic acid was used to detect the structural changes induced by bovine serum albumin (BSA). Here, we conclude that BSA assists in the refolding and regain of LdTIM enzyme activity by providing framework for structure formation. This study indicates that numerous protein-protein contacts are constantly occurring inside the cell that leads to the formation of native protein.


Kumar K,Bhargava P,Roy U




Has Abstract


2011-08-01 00:00:00












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    journal_title:Applied biochemistry and biotechnology

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    authors: Crofcheck C,Maiti I,Pattanaik S,Jay M

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  • Anticancer and DNA binding activities of platinum (IV) complexes; importance of leaving group departure rate.

    abstract::The two six-coordinate Pt(IV) complexes, containing bidentate nitrogen donor/methyl ligands with general formula [Pt(X)2Me2((t)bu2bpy)], where (t)bu2bpy = 4,4'-ditert-butyl-2,2'-bipyridine and X = Cl (C1) or Br (C2), serving as the leaving groups were synthesized for evaluation of their anticancer activities and DNA b...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pouryasin Z,Yousefi R,Nabavizadeh SM,Rashidi M,Hamidizadeh P,Alavianmehr MM,Moosavi-Movahedi AA

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    authors: DeSilva BS,Orosz G,Egodage KL,Carlson RG,Schowen RL,Wilson GS

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    journal_title:Applied biochemistry and biotechnology

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    authors: Tang XS,Tang ZR,Wang SP,Feng ZM,Zhou D,Li TJ,Yin YL

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ben Rejeb I,Monser L,Gargouri M

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    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Tang B,Bi W,Row KH

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  • Statistical optimization of alpha-amylase production with immobilized cells of Streptomyces erumpens MTCC 7317 in Luffa cylindrica L. sponge discs.

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    journal_title:Applied biochemistry and biotechnology

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    authors: Kar S,Swain MR,Ray RC

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    authors: Mogolloń L,Rodríguez R,Larrota W,Ortiz C,Torres R

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    pub_type: 杂志文章,评审


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    journal_title:Applied biochemistry and biotechnology

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    authors: Michalak I,Chojnacka K

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    authors: Arora A,Dien BS,Belyea RL,Wang P,Singh V,Tumbleson ME,Rausch KD

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  • Modulatory Effect of Indoles on the Expression of miRNAs Regulating G1/S Cell Cycle Phase in Breast Cancer Cells.

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    authors: Vaidyanathan J,Bhathena-Langdana Z,Adivarekar RV,Nerurkar M

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    pub_type: 杂志文章


    authors: Kim SM,Park H,Choi JI

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    更新日期:2014-01-01 00:00:00