Continuous Production of Isomalto-oligosaccharides by Thermo-inactivated Cells of Aspergillus niger J2 with Coarse Perlite as an Immobilizing Material.

Abstract:

:The coarse perlite 40-80 mesh was selected as an immobilizing material and put into a packed bed reactor (PBR) to continuously convert maltose to isomalto-oligosaccharides (IMOs). The PBR was prepared by mixing the thermo-inactivated cells (TIC) from Aspergillus niger J2 strain with the coarse perlite, then the mixture was put into an overpressure-resistant column. Compared with diatomite 40-80 mesh and thin perlite 80-120 mesh in PBR, coarse perlite was chosen as the best filtration aid, when the ratio of coarse perlite versus TIC was 1:1. The thermal and pH stability of the free and immobilized TIC and the optimum conditions for the transglycosylation reactions were determined. The results show that approximately 75 and 82% and 87 and 91% of α-glucosidase activity were reserved for free and immobilized TIC at temperatures from 30 to 60 °C and pH from 3.00 to 7.00 for 12 h, respectively. With 30% malt syrup under the conditions of 50 °C and pH 4.00, a mini-scale packed bed reactor (Mi-PBR) and medium-scale packed bed reactor (Me-PBR) could continuously produce IMO over 25 and 34 days with the yield of effective IMO (eIMO) ≥ 35% and total IMO (tIMO) ≥ 50%, respectively. The strategy of mixing the coarse perlite with TIC in PBR is a novel approach to continuously produce IMO and has great application potential in industry.

authors

Huang Z,Li Z,Su Y,Zhu Y,Zeng W,Chen G,Liang Z

doi

10.1007/s12010-018-2706-6

subject

Has Abstract

pub_date

2018-08-01 00:00:00

pages

1088-1099

issue

4

eissn

0273-2289

issn

1559-0291

pii

10.1007/s12010-018-2706-6

journal_volume

185

pub_type

杂志文章
  • Global Transcriptome Profiling of Enterobacter Strain NRS-1 in Response to Hydrogen Peroxide Stress Treatment.

    abstract::Microbes are often subjected to oxidative stress in nature that badly affects their growth rate and viability. Although the response of microbes against oxidative stress has been characterized at the chemical, physiological, and molecular levels, the mechanism of gene-regulation network adaptations of bacteria in resp...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03313-x

    authors: Fei YY,Bhat JA,Gai JY,Zhao TJ

    更新日期:2020-08-01 00:00:00

  • The Effect of Ionic Liquid Pretreatment on the Bioconversion of Tomato Processing Waste to Fermentable Sugars and Biogas.

    abstract::Tomato pomace is an abundant lignocellulosic waste stream from industrial tomato processing and therefore a potential feedstock for production of renewable biofuels. However, little research has been conducted to determine if pretreatment can enhance release of fermentable sugars from tomato pomace. Ionic liquids (ILs...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-016-2061-4

    authors: Allison BJ,Cádiz JC,Karuna N,Jeoh T,Simmons CW

    更新日期:2016-08-01 00:00:00

  • Isolation, purification, and characterization of two thermostable endo-1,4-β-D-glucanase forms from Opuntia vulgaris.

    abstract::Four endoglucanase temperature isoforms (T (30), T (50), T (70), and T (90)) were identified and purified from the cladodes of the xerophytic plant Opuntia vulgaris. These isoforms exhibited optimum catalytic activity at 30 °C, 50 °C, 70 °C, and 90 °C and yielded an apparent molecular mass of 150, 20, 74, and 45 kDa, ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-011-9380-2

    authors: Shyamala S,Ravikumar S,Vikramathithan J,Srikumar K

    更新日期:2011-12-01 00:00:00

  • 6-Tosyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide analogues: synthesis, characterization, MO calculation, and antibacterial activity.

    abstract::A series of 6-tosyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide analogues are synthesized by conventional techniques and characterized by elemental analysis, IR, MS, (1)H, and (13)C NMR. These are tested for their antibacterial activity against Bacillus subtilis (abbreviated as BS), Staphylococcus aureus (ab...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-1399-8

    authors: Doshi H,Thakkar S,Khirsariya P,Thakur MC,Ray A

    更新日期:2015-02-01 00:00:00

  • Properties of a thermostable nonspecific fructofuranosidase produced by Cladosporium cladosporioides cells for hydrolysis of Jerusalem artichoke extract.

    abstract::Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02922120

    authors: Ferreira MS,De Andrade AV,Kennedy JF

    更新日期:1991-10-01 00:00:00

  • The effects of engineering design on heterogeneous biocatalysis in microchannels.

    abstract::The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilize...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-9103-x

    authors: Jones F,Bailey R,Wilson S,Hiestand J

    更新日期:2007-04-01 00:00:00

  • A different method of measuring and detecting monoand dioxygenase activities: key enzymes in hydrocarbon biodegradation.

    abstract::A spectrophotometric method of measuring oxygenase activity in cell extracts or in zymograms was developed. It is an easy and cheap method that allows spectrophotometric measurement of activity by a colored reaction and reveals activity bands in a polyacrylamide gel electrophoresis (PAGE) gel as brown bands. To prove ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:108:1-3:725

    authors: Zazueta-Sandoval R,Novoa VZ,Jiménez HS,Ortiz RC

    更新日期:2003-04-01 00:00:00

  • Molecular modification of Protein A to improve the elution pH and alkali resistance in affinity chromatography.

    abstract::Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mut...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0818-1

    authors: Xia HF,Liang ZD,Wang SL,Wu PQ,Jin XH

    更新日期:2014-04-01 00:00:00

  • Metagenomic Insights in Activated Biomass Treating Industrial Wastewater at Different DO Levels.

    abstract::Dissolved oxygen (DO) is an imperative parameter of the activated sludge process (ASP) for wastewater bioremediation. The effect of DO on microbial communities and corresponding metabolic functions in wastewater bioremediation was investigated using next-generation analysis techniques in this study. Illumina-based who...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03340-8

    authors: Yadav TC,Jadeja NB,Kapley A

    更新日期:2020-10-01 00:00:00

  • Development and application of a system for analysis of mixed cultures of microorganisms.

    abstract::Development and application of a system for real-time quantitative assessment of individual cell activities in a mixed culture system was investigated. This was based on a concept that the activities of individual cells in a mixed culture can be assessed if the cells are physically separated (in separate compartments)...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:80:1:51

    authors: Tanaka H,Ebata T,Kuwahara I,Matsuo M,Ogbonna JC

    更新日期:1999-04-01 00:00:00

  • Microwave-Assisted Biodiesel Production from Microalgae, Scenedesmus Species, Using Goat Bone-Made Nano-catalyst.

    abstract::The production of biodiesel from Scenedesmus algal oil is one of the best alternative forms of liquid fuel production from biomass to petrol diesel. Biodiesel plays a significant role in the carbon sequestration process during cultivation. Scenedesmus algal species was isolated and cultured in a bold basal medium by u...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03149-0

    authors: Mamo TT,Mekonnen YS

    更新日期:2020-04-01 00:00:00

  • Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis.

    abstract::This article demonstrates the potential of encapsulated, engineered Lactococcus lactis as a vehicle for the oral delivery of therapeutic proteins. Using alginate-poly-l-lysine-alginate membrane-encapsulated L. lactis engineered to secrete the reporter protein Staphylococcal aureus nuclease, we show comparable viabilit...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-0002-y

    authors: Lawuyi B,Chen H,Afkhami F,Kulamarva A,Prakash S

    更新日期:2007-07-01 00:00:00

  • Isolation and screening of microorganisms for R-(+)-limonene and (-)-beta-pinene biotransformation.

    abstract::This work is focused on the biotransformation of R-(+)-limonene and (-)-beta-pinene to bioflavor production. To carry out the present study, 405 microorganisms were tested for their ability to bioconvert the substrates. From the isolated microorganisms, 193 were selected in the prescreening using mineral medium for li...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8872-9

    authors: Rottava I,Cortina PF,Grando CE,Colla AR,Martello E,Cansian RL,Toniazzo G,Treichel H,Antunes OA,Oestreicher EG,de Oliveira D

    更新日期:2010-10-01 00:00:00

  • A comparison of the formation of intermediary products during lactose hydrolysis with free and immobilized thermophilic lactase.

    abstract:CONCLUSION:Immobilization of the thermophilic lactase causes a decrease in the formation of intermediary products. This means that partially hydrolyzed lactose syrups contain less of these intermediary products when the immobilized thermophilic lactase is used in the hydrolysis process than when a soluble lactase is us...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02798615

    authors: Rugh S

    更新日期:1982-01-01 00:00:00

  • Diacetyl production mechanism by a strain of Lactococcus lactis spp. lactis bv. diacetylactis. Study of alpha-acetolactic acid extracellular accumulation under anaerobiosis.

    abstract::Diacetyl production via alpha-acetolactic acid (ALA) extracellular decarboxylation in Lactococcus lactis spp. lactis bv. diacetylactis SD 933 cultures has been assessed under anaerobiosis both in batch and continuous fermentations at pH 5.5 and 8.0 by studying the effects of alpha-acetolactate decarboxylase (ADC) addi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02919393

    authors: Rondags E,Halliday E,Marc I

    更新日期:1998-02-01 00:00:00

  • Synergistic Anticancer Effect of a Combination of Paclitaxel and 5-Demethylnobiletin Against Lung Cancer Cell Line In Vitro and In Vivo.

    abstract::Lung cancer remains a highly prevalent disease and a leading cause of cancer-related deaths worldwide. Currently, exploring antitumor drugs derived from herbs used in traditional Chinese medicine is increasingly becoming an attractive area of research. Paclitaxel (PTX), a highly effective chemotherapeutic drug, is wid...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-018-2869-1

    authors: Tan KT,Li S,Li YR,Cheng SL,Lin SH,Tung YT

    更新日期:2019-04-01 00:00:00

  • Rational design of catechol-2, 3-dioxygenase for improving the enzyme characteristics.

    abstract::Catechol-2, 3-dioxygenase (C23O) from Pseudomonas sp. CGMCC2953 identified in our laboratory, which is one of the key enzymes responsible for phenanthrene biodegradation, was expected to get better characteristics tolerant to environment for its further application. With the aim of improving the enzyme properties by i...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8720-y

    authors: Wei J,Zhou Y,Xu T,Lu B

    更新日期:2010-09-01 00:00:00

  • High Productivity Ethanol from Solid-State Fermentation of Steam-Exploded Corn Stover Using Zymomonas mobilis by N2 Periodic Pulsation Process Intensification.

    abstract::Solid-state fermentation, featured by water-saving, eco-friendly and high concentration product, is a promising technology in lignocellulosic ethanol industry. However, in solid-state fermentation system, large gas content inside the substrate directly leads to high oxygen partial pressure and inhibits ethanol ferment...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03318-6

    authors: Sun L,Wang L,Chen H

    更新日期:2020-10-01 00:00:00

  • Polyhydroxybutyrate/Chitosan 3D Scaffolds Promote In Vitro and In Vivo Chondrogenesis.

    abstract::The articular cartilage is an avascular and aneural tissue and its injuries result mostly in osteoarthritic changes and formation of fibrous tissue. Efforts of scientists worldwide are focused on restoration of cartilage with increase in life quality of patients. Novel polymeric polyhydroxybutyrate/chitosan (PCH) poro...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-019-03021-1

    authors: Giretova M,Medvecky L,Petrovova E,Cizkova D,Danko J,Mudronova D,Slovinska L,Bures R

    更新日期:2019-10-01 00:00:00

  • Study on photoconductivity of dye-polymer-based solid-state thin film.

    abstract::In this article, we describe a solid-state photoelectrochemical cell for light detection. Safranine-T dye mixed with polyvinyl alcohol (PVA) solution was deposited on a conducting and transparent indium-tin-oxide (ITO)-coated glass that was used as one electrode and another ITO-coated glass as the counterelectrode. A ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:96:1-3:055

    authors: Dey SK,Manik NB

    更新日期:2001-10-01 00:00:00

  • Anaerobic reduction of a sulfonated azo dye, Congo Red, by sulfate-reducing bacteria.

    abstract::The capacity for anaerobic decolorization of a sulfonated azo dye, Congo Red, by a strain of a sulfate-reducing bacterium was evaluated. After optimizing the growth rate of the bacteria on a simple carbon source and terminal electron acceptor pair, lactate and sulfate, respectively, the effect of the dye concentration...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:97:3:147

    authors: Diniz PE,Lopes AT,Lino AR,Serralheiro ML

    更新日期:2002-03-01 00:00:00

  • Alpha-glucosidase folding during urea denaturation: enzyme kinetics and computational prediction.

    abstract::In this study, we investigated structural changes in alpha-glucosidase during urea denaturation. Alpha-glucosidase was inactivated by urea in a dose-dependent manner. The inactivation was a first-order reaction with a monophase process. Urea inhibited alpha-glucosidase in a mixed-type reaction. We found that an increa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-009-8636-6

    authors: Wu XQ,Wang J,Lü ZR,Tang HM,Park D,Oh SH,Bhak J,Shi L,Park YD,Zou F

    更新日期:2010-03-01 00:00:00

  • Direct fermentation of gelatinized cassava starch to acetone, butanol, and ethanol using Clostridium acetobutylicum mutant obtained by atmospheric and room temperature plasma.

    abstract::The mutant strain designated as ART18, obtained from the wild-type strain Clostridium acetobutylicum PW12 treated by atmospheric and room temperature plasma, showed higher solvent tolerance and butanol production than that of the wild-type strain. The production of butanol was 11.3 ± 0.5 g/L, 31 % higher than that of ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-014-0765-x

    authors: Li HG,Luo W,Wang Q,Yu XB

    更新日期:2014-04-01 00:00:00

  • Thermophilic fermentation of hydrolysates: the effect of inhibitors on growth of thermophilic bacteria.

    abstract::Lignocellulosic biomass has great potential as a cheap feedstock in biological processes to produce biofuels or chemicals; however, dilute acid pretreatment at high temperatures produces undesirable compounds. Toxicity tests were done with inhibitors in standard media, to predict the growth-limiting effects on thermop...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:98-100:1-9:765

    authors: Thomasser C,Danner H,Neureiter M,Saidi B,Braun R

    更新日期:2002-04-01 00:00:00

  • Properties of bacterial luciferase/NADH : FMN oxidoreductase and firefly luciferase immobilized onto sepharose.

    abstract::NADH : FMN oxidoreductase and bacterial luciferase have been efficiently coimmobilized onto Sepharose 4B. This luminescent immobilized enzyme system can be used to assay NADH. The assay is rapid and sensitive with a lower limit of detection of 0.2 pmol/assay tube. The intra-assay precision was 3.5% at 2 × 10(-5) M and...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/BF02799178

    authors: Wienhausen GK,Kricka LJ,Hinkley JE,Deluca M

    更新日期:1982-11-01 00:00:00

  • Effect of tributylphenyltetraethoxylate on enzyme production of Pleurotus ostreatus.

    abstract::The effect of the addition of the nonionic surfactant tributylphenyltetraethoxylate to culture media on pH and extracellular protein content, and on production of beta-glucosidase, xylanase, laccase, and manganese-dependent and -independent peroxidases by the edible fungus Pleurotus ostreatus was determined. The relat...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1385/abab:110:1:33

    authors: Ramos C,Morais H,Forgács E,Cserháti T

    更新日期:2003-07-01 00:00:00

  • An Innovator Support Material for Tyrosinase Immobilization: Antimony-Doped Tin Oxide Thin Films (ATO-TF).

    abstract::Tyrosinase (T3824, Sigma) enzymes were immobilized onto SnO2: Sb thin films (ATO-TF) which were synthesized in laboratory conditions by spray pyrolysis technique. Immobilization of tyrosinase onto SnO2: Sb thin film (ATO-TF) was confirmed by scanning electron microscopy (SEM) and Fourier transformed infrared spectrosc...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-020-03337-3

    authors: Türkhan A,Kaya ED,Koçyiğit A

    更新日期:2020-10-01 00:00:00

  • Regional differences in rice hulls supply for bioethanol production.

    abstract::Agricultural by-products are becoming an attractive substrate for bioethanol production. The aim of this study was to evaluate the effects of regional differences in the rice hulls using Escherichia coli KO11 for bioethanol production. The rice hulls coded Edirne were obtained from Thrace Region, and the rice hulls co...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-013-0504-8

    authors: Imamoglu E,Dalay MC,Sukan FV

    更新日期:2013-12-01 00:00:00

  • A simple substrate feeding strategy using a pH control trigger in fed-batch fermentation.

    abstract::A simple automated glucose feeding strategy based on pH control was developed to produce high-cell-density fed-batch fermentation. In this strategy, the pH control scheme utilized an acidified concentrated glucose solution to lower the pH. The frequency of glucose addition to the fermentor is determined by the culture...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-8089-8

    authors: Ting TE,Thoma GJ,Beitle RR Jr,Davis RK,Perkins R,Karim K,Liu HM

    更新日期:2008-04-01 00:00:00

  • Evaluation of a Hypocrea jecorina enzyme preparation for hydrolysis of Tifton 85 bermudagrass.

    abstract::Tifton 85 bermudagrass, developed at the ARS-USDA in Tifton, GA, is grown on over ten million acres in the USA for hay and forage. Of the bermudagrass cultivars, Tifton 85 exhibits improved digestibility because the ratio of ether- to ester-linked phenolic acids has been lowered using traditional plant breeding techni...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章

    doi:10.1007/s12010-007-8129-4

    authors: Ximenes EA,Brandon SK,Doran-Peterson J

    更新日期:2008-03-01 00:00:00