Abstract：
:Vacuolar processing enzymes (VPEs) have received considerable attention recently, as they exhibit caspase-1-like cleavage activity and regulate the process of PCD. However, knowledge about their detailed characteristics and structures is relatively limited. In this study, a gamma vacuolar processing enzyme gene, MhVPEγ, has been isolated from the leaves of Malus hupehensis (Ramp) Rehd. var pinyiensis Jiang. MhVPEγ coded-translated protein sequence comprised of 494 amino acids with a signal peptide and a transmembrane helix structure at N-terminal, peptidase_C13 domain, and vacuolar sorting signal at C-terminal. Consequently, genomic walking approach was performed for the isolation of its upstream sequence. Computational analysis demonstrated several motifs of the promoter exhibiting hypothetic MeJA, ABA, and light-induced characteristics, as well as some typical domains universally discovered in promoter, such as TATA-box and CAAT-box. MhVPEγ transcript level was enhanced during wounding treatment, and WUN-motif, as one of the cis-acting regulatory elements existing in the upstream sequence perhaps regulates its expression. In silico-constructed 3D models revealed that MhCPYL successively interacts with MhVPEγ like that of "Induced Fit-Lock and Key" model, providing molecular conformation evidence that CPY is a direct substrate of VPEγ. This study is the first stride to understand the molecular mechanism of VPEγ and CPYL interactions.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Ran K,Yang H,Sun X,Li Q,Jiang Q,Zhang W,Shen Wdoi
10.1007/s12010-014-0867-5subject
Has Abstractpub_date
2014-05-01 00:00:00pages
579-95issue
2eissn
0273-2289issn
1559-0291journal_volume
173pub_type
杂志文章abstract：:The Institute of Gas Technology (IGT) conducted a pilot-scale study at a former manufactured gas plant (MGP) site in New Jersey. The objective of the study was to determine the effectiveness of an innovative chemical/biological treatment process (MGP-REM process) to remediate soils contaminated with polynuclear aromat...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02920473
更新日期：1997-04-01 00:00:00
abstract：:Soaking in aqueous ammonia (SAA) pretreatment was investigated to improve enzymatic digestibility and consequently to increase total fermentable sugar production from barley straw. Various effects of pretreatment process parameters, such as reaction temperature, reaction time, solid:liquid ratio, and ammonia concentra...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0154-x
更新日期：2013-04-01 00:00:00
abstract：:Asphaltenes from a crude oil rich in heavy metals (Castilla crude oil) were fractionated and partially characterized. Biocatalytic modifications of these fractionated asphaltenes by three different hemoproteins: chloro-peroxidase (CPO), cytochrome C peroxidase (Cit-C), and lignin peroxidase (LPO) were evaluated in bot...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02920187
更新日期：1998-04-01 00:00:00
abstract：:Lipase from Rhizomucor miehei (RML) was immobilized onto chitosan support in the presence of some surfactants added at low levels using two different strategies. In the first approach, the enzyme was immobilized in the presence of surfactants on chitosan supports previously functionalized with glutaraldehyde. In the s...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-017-2622-1
更新日期：2018-04-01 00:00:00
abstract：:As technologies for utilizing biomass for fuel and chemical production continue to improve, enzymatic hydrolysis can be run at still higher solids concentrations. For hydrolyses that initially contain little or no free water (10-40% total solids, w/w), the saccharification of insoluble polymers into soluble sugars inv...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-008-8375-0
更新日期：2009-05-01 00:00:00
abstract：:Success in generating catalytic antibodies as enzyme mimics lies in the strategic design of the transition-state analog (TSA) for the reaction of interest, and careful development of screening processes for the selection of antibodies that are catalysts. Typically, the choice of TSA structure is straightforward, and t...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:83:1-3:195
更新日期：2000-01-01 00:00:00
abstract：:Gastric cancer is the second most common cause of cancer-related death worldwide. This study was designed to examine the role of CUGBP1 in cell growth via an RNA interference (RNAi) lentivirus system in gastric cancer cells in vitro. The expression of CUGBP1 was much stronger in gastric cancer tissues than that in adj...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-0937-8
更新日期：2014-07-01 00:00:00
abstract：:A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipett...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:91-93:1-9:109
更新日期：2001-04-01 00:00:00
abstract：:Diffusion of antibody protein from hydrogel films and hydrogel encapsulated in a microcapillary was studied. Thin hydrogel films were formed by crosslinking 6-acryloyl-B-O-methylgalactoside with N,N'-methylene-bis-acrylamide and the diffusive transport of monoclonal antimouse IgG-FITC into and out of the hydrate films...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/BF02785980
更新日期：1997-10-01 00:00:00
abstract：:Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced an appreciable level of xylanase ac...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:87:2:95
更新日期：2000-05-01 00:00:00
abstract：:Lipases are one of the highest value commercial enzymes as they have broad applications in detergent, food, pharmaceutical, and dairy industries. To provide chimeric Bacillus thermocatenulatus lipase (BTL2), the completely conserved pentapeptide (¹¹²Ala-His-Ser-Gln-Gly¹¹⁶) was replaced with similar sequences (²⁰⁷Gly-G...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-012-0014-0
更新日期：2013-02-01 00:00:00
abstract：:The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 6...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-018-2725-3
更新日期：2018-09-01 00:00:00
abstract：:Since the adult mammalian heart has limited regenerative capacity, cardiac trauma, disease, and aging cause permanent loss of contractile tissue. This has fueled the development of stem cell-based strategies to provide the damaged heart with new cardiomyocytes. Bone marrow-derived mesenchymal stem cells (BM-MSCs) are ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-018-2734-2
更新日期：2018-09-01 00:00:00
abstract：:A newly isolated bacterial strain, Bacillus sp. MX47, was actively producing extracellular xylanase only in xylan-containing medium. The xylanase was purified from the culture broth by two chromatographic steps. The xylanase had an apparent molecular weight of 26.4 kDa with an NH(2)-terminal sequence (Gln-Gly-Gly-Asn-...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-012-9828-z
更新日期：2012-10-01 00:00:00
abstract：:Proteases constitute one of the most important groups of industrial enzymes, accounting for at least 25% of the total enzyme sales, with two-thirds of the proteases produced commercially being of microbial origin. Immobilized enzymes are currently the subject of considerable interest because of their advantages over s...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：
更新日期：2006-04-01 00:00:00
abstract：:The results of a numerical study of the fundamental interactions of engineering design and micromixing on conversion in packed microchannels are presented. Previously, channel-based microreactors made of molded silicon plastic were designed, fabricated, and experimentally tested. These reactors have enzymes immobilize...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-007-9103-x
更新日期：2007-04-01 00:00:00
abstract：:A comparative analysis of the structural and functional aspects along with equilibrium unfolding of two homologous cutinases, Cut1 and Cut2, from Thermobifida fusca was carried out. The CD and fluorescence profile at different pH in the range of 6 to 9 showed no structural variations for both cutinases, indicating the...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-1037-5
更新日期：2014-09-01 00:00:00
abstract：:Lignocellulosic feedstocks corn stover, prairie cord grass, and switchgrass were subjected to ammonia fiber expansion (AFEX™) pretreatment and densified using extrusion pelleting and ComPAKco densification technique. The effects of AFEX™ pretreatment and densification were studied on the fast pyrolysis product yields....
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2269-3
更新日期：2017-03-01 00:00:00
abstract：:The production of a thermophilic 2-deoxyribose-5-phosphate aldolases (DERA) in Escherichia coli BL21 under continuous lactose induction strategy was investigated. The process was combined with the exponential feeding method, controlling the feeding rate to maintain the specific growth rate at 0.15 h(-1). The results i...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-011-9261-8
更新日期：2011-09-01 00:00:00
abstract：:The main goal of this paper was to elaborate the possibility of industrial application of biosorption properties of Enteromorpha prolifera (production of mineral feed additives for livestock). In this study, biosorption process was used in the binding of chromium(III) ions from aqueous solution by the green macroalga....
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-009-8635-7
更新日期：2010-03-01 00:00:00
abstract：:An extracellular polygalacturonase (PG) produced from Paecilomyces variotii was purified to homogeneity through two chromatography steps using DEAE-Fractogel and Sephadex G-100. The molecular weight of P. variotii PG was 77,300 Da by gel filtration and SDS-PAGE. PG had isoelectric point of 4.37 and optimum pH 4.0. PG ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-009-8682-0
更新日期：2010-03-01 00:00:00
abstract：:Ethanol from corn is produced using dry grind corn process in which simultaneous saccharification and fermentation (SSF) is one of the most critical unit operations. In this work an optimal controller based on a previously validated SSF model was developed by formulating the SSF process as a Bolza problem and using gr...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-011-9406-9
更新日期：2012-01-01 00:00:00
abstract：:Superoxide dismutase (SOD, EC 1.15.1.1) is an enzyme catalyzing the dismutation of superoxide radical to hydrogen peroxide and dioxygen. To date, four types of SODs--Cu/ZnSOD, MnSOD, FeSOD, and NiSOD--have been identified. In this study, SOD proteins of Brachypodium distachyon (L.) Beauv. were screened by utilization ...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-014-0922-2
更新日期：2014-07-01 00:00:00
abstract：:When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of de...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-010-9112-z
更新日期：2011-05-01 00:00:00
abstract：:Biological control of soil-borne plant pathogens is a potential alternative to the use of chemical pesticides, which have already been proved to be harmful to the environment. Several strains of the fungus Trichoderma have been isolated and found to be effective biocontrol agents of various soil-borne plant pathogenic...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章,评审
doi：10.1007/BF02825358
更新日期：1994-07-01 00:00:00
abstract：:Lysozymes are known as ubiquitously distributed immune effectors with hydrolytic activity against peptidoglycan, the major bacterial cell wall polymer, to trigger cell lysis. In the present study, the full-length cDNA sequence of a novel sea urchin Strongylocentrotus purpuratus invertebrate-type lysozyme (sp-iLys) was...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-018-2753-z
更新日期：2018-10-01 00:00:00
abstract：:Based on the principle of self-coagulation of microorganisms, the flocculant-producing denitrifying bacterial TN-14 sludge was added to the continuous-flow reactor for treating domestic sewage. The bacterial TN-14 sludge acted as the main seed sludge to promote the rapid formation of aerobic granular sludge. The sludg...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-016-2221-6
更新日期：2017-01-01 00:00:00
abstract：:Two DNA fragments containing the entire coding sequences of lactate dehydrogenase (LDH; ldhL1 and ldhD), whose enzymes have high activity for bioconversion of phenylpyruvate (PPA) to phenyllactate (PLA), were amplified from Lactobacillus plantarum SK002 using PCR. Sequencing showed open reading frames of 963 bp (ldhL1...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-009-8767-9
更新日期：2010-09-01 00:00:00
abstract：:In the present study, effects of aqueous extracts from Crocodylus siamensis bile (AE-CB) on SMMC-7721 cell growth, cell cycle, and apoptosis were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, inverted microscopy, fluorescence microscopy, propidium iodide (PI) single- and fluoresc...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1007/s12010-013-0120-7
更新日期：2013-05-01 00:00:00
abstract：:Development and application of a system for real-time quantitative assessment of individual cell activities in a mixed culture system was investigated. This was based on a concept that the activities of individual cells in a mixed culture can be assessed if the cells are physically separated (in separate compartments)...
journal_title：Applied biochemistry and biotechnology
pub_type： 杂志文章
doi：10.1385/abab:80:1:51
更新日期：1999-04-01 00:00:00
