Structural stability and unfolding properties of cutinases from Thermobifida fusca.

abstract：:To investigate the effect of pH and temperature on the cell growth and bacteriocin production of Pediococcus acidilactici PA003, a lactic acid bacterium isolated from traditionally fermented cabbage, the kinetic behaviour of P. acidilactici PA003 was simulated in vitro during laboratory fermentations by making use of ...

journal_title：Applied biochemistry and biotechnology

authors： Zhang J,Zhang Y,Liu SN,Han Y,Zhou ZJ

更新日期：2012-03-01 00:00:00

abstract：:Lignocellulose biomass contain large macromolecules especially cellulose and hemicelluloses that can be converted to fuel and chemicals using microbial biocatalysts. This study presents comprehensive optimization of production of biomass-hydrolyzing enzymes (BHE) by a high β-glucosidase-producing Trichoderma SG2 for b...

journal_title：Applied biochemistry and biotechnology

authors： Nanjundaswamy A,Okeke BC

更新日期：2020-05-01 00:00:00

abstract：:EDTA, the target compound of this study from the effluent of secondary biotreatment units, can be biodegraded by special microorganisms. So far, there are three species of microorganisms--Agrobacterium, Gram-negative BNCI, and DSM9103--that can degrade EDTA and are published in the literature. We have successfully iso...

journal_title：Applied biochemistry and biotechnology

authors： Fang HY,Chen SC,Chen SL

更新日期：2003-11-01 00:00:00

abstract：:Bacterial strain NYT501, which we previously isolated from soil, was identified as Stenotrophomonas maltophilia, and it was confirmed that this strain produces an intracellular β-N-acetylhexosaminidase exhibiting transglycosylation activity. Several properties of this enzyme were characterized using a partially purifi...

journal_title：Applied biochemistry and biotechnology

authors： Uehara A,Takahashi N,Moriyama M,Hirano T,Hakamata W,Nishio T

更新日期：2018-02-01 00:00:00

abstract：:Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant...

journal_title：Applied biochemistry and biotechnology

authors： Gurjar K,Rampuria S,Joshi U,Palit P,Bhatt KV,Mohapatra T,Sharma R

更新日期：2014-08-01 00:00:00

abstract：:The inhibitory effect of L-malic acid (MA) on alpha-glucosidase (EC 3.2.1.20) was investigated by combination study between inhibition kinetics and computational simulations. The results from the serial kinetics demonstrated that MA could directly inactivate the enzyme activity in a dose-dependent manner and a typical...

journal_title：Applied biochemistry and biotechnology

authors： Gou L,Zhan Y,Lee J,Li X,Lü ZR,Zhou HM,Lu H,Wang XY,Park YD,Yang JM

更新日期：2015-02-01 00:00:00

abstract：:The abilities of lignin peroxidase (LIP) and manganese peroxidase (MNP) from Phanerochaete chrysosporium to degrade an insoluble hardwood lignin in vitro in aqueous media were tested. Neither LIP nor MNP appreciably changed the mass or lignin content, although both produced small amounts of unique solubilized lignin f...

journal_title：Applied biochemistry and biotechnology

authors： Thompson DN,Hames BR,Reddy CA,Grethlein HE

更新日期：1998-04-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:A novel expression system of Klebsiella pneumoniae was developed in order to improve 1,3-propanediol (1,3-PD) production using a K. pneumoniae-Escherichia coli shuttle vector pET28a consisting of the kanamycin-resistance gene promoter Pkan. The recombinant plasmid pETPkan-cat carrying the chloramphenicol acetyltransfe...

journal_title：Applied biochemistry and biotechnology

authors： Ma Z,Rao Z,Zhuge B,Fang H,Liao X,Zhuge J

更新日期：2010-09-01 00:00:00

abstract：:NADH : FMN oxidoreductase and bacterial luciferase have been efficiently coimmobilized onto Sepharose 4B. This luminescent immobilized enzyme system can be used to assay NADH. The assay is rapid and sensitive with a lower limit of detection of 0.2 pmol/assay tube. The intra-assay precision was 3.5% at 2 × 10(-5) M and...

journal_title：Applied biochemistry and biotechnology

authors： Wienhausen GK,Kricka LJ,Hinkley JE,Deluca M

更新日期：1982-11-01 00:00:00

abstract：:Recently, poly(3-hydroxybutyrate) (PHB) has been found in a few thermophilic strains where several advantages can be gained from running fermentation at high temperatures. Caldimonas manganoxidans, a thermophilic gram-negative bacterium, was investigated for the feasibility as a PHB-producing strain. It is suggested t...

journal_title：Applied biochemistry and biotechnology

authors： Hsiao LJ,Lin JH,Sankatumvong P,Wu TM,Li SY

更新日期：2016-11-01 00:00:00

abstract：:Cofermentation of glucose, xylose, and arabinose is critical for complete bioconversion of lignocellulosic biomass, such as agricultural residues and herbaceous energy crops, to ethanol. We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, a...

journal_title：Applied biochemistry and biotechnology

authors： Mohagheghi A,Evans K,Chou YC,Zhang M

更新日期：2002-04-01 00:00:00

abstract：:Polyhydroxyalkanoates, biodegradable plastics with the desired physical and chemical properties of conventional synthetic plastics, are extensively investigated. In this study, specific bacterial strains produced specific copolymers from food waste. Copolymers of HB and HV (poly[3-hydroxybutyrate-co-3-hydroxyvalerate]...

journal_title：Applied biochemistry and biotechnology

authors： Wong PA,Chua H,Lo W,Lawford HG,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Bovine lactoferrampin (LFA) and bovine lactoferricin (LFC) are two antimicrobial peptides located in the N(1) domain of bovine lactoferrin. The bactericidal activity of the fused peptide LFA-LFC is stronger than that of either LFA or LFC. The high cost of peptide production from either native digestion or chemical syn...

journal_title：Applied biochemistry and biotechnology

authors： Tang XS,Tang ZR,Wang SP,Feng ZM,Zhou D,Li TJ,Yin YL

更新日期：2012-02-01 00:00:00

abstract：:Magnetosomes are specialized organelles arranged in intracellular chains in magnetotactic bacteria. The superparamagnetic property of these magnetite crystals provides potential applications as contrast-enhancing agents for magnetic resonance imaging. In this study, we compared two different nanoparticles that are bac...

journal_title：Applied biochemistry and biotechnology

authors： Erdal E,Demirbilek M,Yeh Y,Akbal Ö,Ruff L,Bozkurt D,Cabuk A,Senel Y,Gumuskaya B,Algın O,Colak S,Esener S,Denkbas EB

更新日期：2018-05-01 00:00:00

abstract：:An efficient regeneration system was established for an ethnomedicinal shrub Rhinacanthus nasutus from root-derived callus organogenesis. The root segments were cultured on MS medium supplemented with various concentrations of Kn (1.0-4.0 μM) alone or in combination with IBA (0.2-0.6 μM) or 2, 4-D (0.5-1.5 μM). The op...

journal_title：Applied biochemistry and biotechnology

authors： Cheruvathur MK,Thomas TD

更新日期：2014-02-01 00:00:00

abstract：:Seven white rot fungal species were tested for growth as mycelia using swine wastewater (SW), an agro-waste with tremendous environmental footprint, as the sole nutrient source. The SW contained high concentrations of carbon and nitrogen components, which could support nutritional requirements for mycelial growth. Out...

journal_title：Applied biochemistry and biotechnology

authors： Lee J,Shin SG,Ahn J,Han G,Hwang K,Kim W,Hwang S

更新日期：2017-02-01 00:00:00

abstract：:The autoimmune repertoire is well known from previous studies to be capable of producing catalytic antibodies directed to self-antigens. In the present study, we explored the ability of 26 monoclonal light chains (L chains) from multiple myeloma patients to cleave radiolabeled gp120, a foreign protein. One L chain wit...

journal_title：Applied biochemistry and biotechnology

authors： Paul S,Kalaga RS,Gololobov G,Brenneman D

更新日期：2000-01-01 00:00:00

abstract：:The genome sequence, morphology, and genetic features of a novel phage, named SSE1, is reported here. Phage SSE1 that infects Shigella dysenteriae (China General Microbiological Culture Collection Center number: 1.1869) was isolated from the aeration tank water of a sewage treatment plant. SSE1 showed morphological fe...

journal_title：Applied biochemistry and biotechnology

authors： Lu H,Liu H,Lu M,Wang J,Liu X,Liu R

更新日期：2020-09-01 00:00:00

abstract：:Tannase production by newly isolated Penicillium purpurogenum PAF6 was investigated by 'one variable at a time' (OVAT) approach followed by response surface methodology (RSM). Tannin-rich plant residues were used as supporting solid substrate and sole carbon source and, among them, tamarind seed was found to be the mo...

journal_title：Applied biochemistry and biotechnology

authors： Jana A,Maity C,Halder SK,Mondal KC,Pati BR,Mohapatra PK

更新日期：2012-07-01 00:00:00

abstract：:Crosslinked enzyme aggregates (CLEAs) of a partially purified penicillin acylase from a recombinant Escherichia coli strain have been produced as a novel type of biocatalysts well endowed to perform in organic media. Different protein precipitants were studied and glutaraldehyde was used as the crosslinking agent. Pre...

journal_title：Applied biochemistry and biotechnology

authors： Illanes A,Wilson L,Caballero E,Fernández-Lafuente R,Guisán JM

更新日期：2006-06-01 00:00:00

abstract：:In this work, important results from simulations are presented, showing the potentiality of the molecular distillation process for recovering vitamin E from vegetal oils. Two types of molecular distillators are considered: falling film and centrifugal. The results emphasize the degree of recovery and factors that infl...

journal_title：Applied biochemistry and biotechnology

authors： Batistella CB,Moraes EB,Maciel Filho R,Maciel MR

更新日期：2002-04-01 00:00:00

abstract：:The effect of temperature on the performance of an anaerobic sequencing biofilm batch reactor (ASBBR) with liquid-phase recirculation was assessed. Assays were performed using a recirculation velocity of 0.20 cm/s, 8-h cycles, and an average treated synthetic wastewater volume of 2 L/cycle with a concentration of 500 ...

journal_title：Applied biochemistry and biotechnology

authors： Agibert SA,Moreira MB,Ratusznei SM,Rodrigues JA,Zaiat M,Foresti E

更新日期：2007-02-01 00:00:00

abstract：:Asphaltenes from a crude oil rich in heavy metals (Castilla crude oil) were fractionated and partially characterized. Biocatalytic modifications of these fractionated asphaltenes by three different hemoproteins: chloro-peroxidase (CPO), cytochrome C peroxidase (Cit-C), and lignin peroxidase (LPO) were evaluated in bot...

journal_title：Applied biochemistry and biotechnology

authors： Mogolloń L,Rodríguez R,Larrota W,Ortiz C,Torres R

更新日期：1998-04-01 00:00:00

abstract：:Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

journal_title：Applied biochemistry and biotechnology

authors： Padma PN,Rao AB,Yadav JS,Reddy G

更新日期：2002-07-01 00:00:00

abstract：:Regulation of the cysteine protease activity is imperative for proper functioning of the various organ systems. Elevated activities of cysteine proteinases due to impaired regulation by the endogenous cysteine proteinase inhibitors (cystatins) have been linked to liver malignancies. To gain an insight into these regul...

journal_title：Applied biochemistry and biotechnology

authors： Shah A,Priyadarshini M,Khan MS,Aatif M,Amin F,Bano B

更新日期：2013-10-01 00:00:00

abstract：:Volatile C6-aldehydes are the main contributors to the characteristic odor of plants known as "green note" and are widely used by the flavor industry. Biotechnological processes were developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants. Recombinant hydroperoxide lyases (HPLs) constit...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Mariani M,de Caraffa VB,Gambotti C,Vincenti S,Desjobert JM,Muselli A,Costa J,Berti L,Maury J

更新日期：2016-06-01 00:00:00

abstract：:In this study, we investigated structural changes in alpha-glucosidase during urea denaturation. Alpha-glucosidase was inactivated by urea in a dose-dependent manner. The inactivation was a first-order reaction with a monophase process. Urea inhibited alpha-glucosidase in a mixed-type reaction. We found that an increa...

journal_title：Applied biochemistry and biotechnology

authors： Wu XQ,Wang J,Lü ZR,Tang HM,Park D,Oh SH,Bhak J,Shi L,Park YD,Zou F

更新日期：2010-03-01 00:00:00

abstract：:Soaking in aqueous ammonia (SAA) pretreatment was investigated to improve enzymatic digestibility and consequently to increase total fermentable sugar production from barley straw. Various effects of pretreatment process parameters, such as reaction temperature, reaction time, solid:liquid ratio, and ammonia concentra...

journal_title：Applied biochemistry and biotechnology

authors： Yoo CG,Nghiem NP,Hicks KB,Kim TH

更新日期：2013-04-01 00:00:00

abstract：:Expression of exogenous DNA in vitro is significantly affected by the particular transfection method utilized. In this study, we evaluated the efficiency of two transfection methods, chemically mediated polyethyleneimine (PEI) treatment and physically mediated electroporation, on a rat heart myoblast cell line, H9c2(2...

journal_title：Applied biochemistry and biotechnology

authors： Liu YC,Lin WY,Jhang YR,Huang SH,Wu CP,Wu HT

更新日期：2011-08-01 00:00:00