Production of fumaric acid from L-malic acid by solvent engineering using a recombinant thermostable fumarase from Thermus thermophilus HB8.

abstract：:The enzyme mannose isomerase (EC 5.3.1.7) catalyzes the isomerization of D-mannose and D-fructose. The conversion of mannose to fructose is the first step in the principal pathway of mannose dissimilation by Pseudomonas cepacia. This enzyme is induced during growth on medium containing mannose to levels three- to four...

journal_title：Applied biochemistry and biotechnology

authors： Allenza P,Morrell MJ,Detroy RW

更新日期：1990-04-01 00:00:00

abstract：:Lipase-catalyzed esterification of selected phenolic acids with linolenyl alcohols was investigated in selected organic solvent media. The enzyme activity for the esterification of dihydrocaffeic acid with linolenyl alcohol in solvent mixtures of hexane/2-butanone of 75:25 (v/v) and 65:35 (v/v) was 0.88 and 0.47 micro...

journal_title：Applied biochemistry and biotechnology

authors： Sabally K,Karboune S,Yeboah FK,Kermasha S

更新日期：2005-10-01 00:00:00

abstract：:In the present investigation, thermophilic fungus Rhizomucor pusillus was used to study biotransformation of antihelmintic drug albendazole to produce its active metabolite, albendazole sulfoxide and novel metabolites of commercial interest. A two-stage fermentation procedure was followed for biotransformation of albe...

journal_title：Applied biochemistry and biotechnology

authors： Prasad GS,Girisham S,Reddy SM

更新日期：2011-11-01 00:00:00

abstract：:The present study deals with the characterization of halotolerant protease produced by Bacillus aquimaris VITP4 strain isolated from Kumta coast, Karnataka, India. The studies were performed at 40 °C and pH 8 in Tris buffer. Metal ions such as Mn(2+) and Ca(2+) increased the proteolytic activity of the enzyme by 34 an...

journal_title：Applied biochemistry and biotechnology

authors： Thaz CJ,Jayaraman G

更新日期：2014-01-01 00:00:00

abstract：:Protein A of Staphylococcus aureus has been widely used as an affinity ligand for the purification of immunoglobulin. However, the low elution pH and the sensitivity to alkaline condition restricted the large-scale application of antibody purification. To overcome these disadvantages, the B domain was selected and mut...

journal_title：Applied biochemistry and biotechnology

authors： Xia HF,Liang ZD,Wang SL,Wu PQ,Jin XH

更新日期：2014-04-01 00:00:00

abstract：:Moth bean (Vigna aconitifolia (Jacq.) Marechal), an important grain-legume crop grown in hot desert regions of Thar, under scorching sun rays, was investigated for heat tolerance at molecular level. In the present study, we constructed a forward suppression subtractive hybridization (SSH) cDNA library of heat tolerant...

journal_title：Applied biochemistry and biotechnology

authors： Gurjar K,Rampuria S,Joshi U,Palit P,Bhatt KV,Mohapatra T,Sharma R

更新日期：2014-08-01 00:00:00

abstract：:The native amino acid ergothioneine, a thiourea derivative of histidine, inhibits mushroom tyrosinase activity in a dose-dependent manner, with an IC(50) value of 1.025 mg/ml (4.47 mM). By contrast, histidine exhibited no inhibitory effect on mushroom tyrosinase activity. We characterized ergothioneine as a noncompeti...

journal_title：Applied biochemistry and biotechnology

authors： Liao WC,Wu WH,Tsai PC,Wang HF,Liu YH,Chan CF

更新日期：2012-01-01 00:00:00

abstract：:This study investigated the effects of extremely low frequency (ELF) magnetic field with/without iron(III) chloride (FeCl3) on bacterial growth and morphology. The ELF exposures were carried out using a pair of Helmholtz coil-based ELF exposure system which was designed to generate 50 Hz sinusoidal magnetic field. The...

journal_title：Applied biochemistry and biotechnology

authors： Esmekaya MA,Acar SI,Kıran F,Canseven AG,Osmanagaoglu O,Seyhan N

更新日期：2013-04-01 00:00:00

abstract：:In this article, we describe a solid-state photoelectrochemical cell for light detection. Safranine-T dye mixed with polyvinyl alcohol (PVA) solution was deposited on a conducting and transparent indium-tin-oxide (ITO)-coated glass that was used as one electrode and another ITO-coated glass as the counterelectrode. A ...

journal_title：Applied biochemistry and biotechnology

authors： Dey SK,Manik NB

更新日期：2001-10-01 00:00:00

abstract：:Androstenedione (AD) is a steroid intermediate used in the pharmaceutical industry for the production of several important anabolic drugs. An important route for producing AD is by the microbial transformation of sterols. Microbes capable of efficiently transforming sterols to AD are few and newer strains need to be i...

journal_title：Applied biochemistry and biotechnology

authors： Malaviya A,Gomes J

更新日期：2009-08-01 00:00:00

abstract：:Acetone, butanol, ethanol (ABE, or solvents) were produced from starch-based packing peanuts in batch and continuous reactors. In a batch reactor, 18.9 g/L of total ABE was produced from 80 g/L packing peanuts in 110 h of fermentation. The initial and final starch concentrations were 69.6 and 11.1 g/L, respectively. I...

journal_title：Applied biochemistry and biotechnology

authors： Ezeji TC,Groberg M,Qureshi N,Blaschek HP

更新日期：2003-04-01 00:00:00

abstract：:Neoadjuvant chemotherapy (NACT) is known to be beneficial for patients with locally advanced breast cancer. However, there is still no unified standard on the evaluation of NACT. To identify the potential markers related to NACT sensitivity of breast cancer, in the present study, we examined the protein spectrum of br...

journal_title：Applied biochemistry and biotechnology

authors： Zhang K,Yuan K,Wu H,Li Q,Wang Y,Chen S,Zhang L,Gu H,Fu R

更新日期：2012-02-01 00:00:00

abstract：:In addition to fermentable sugars, dilute-acid hydrolysates of lignocellulose contain compounds that inhibit fermenting microorganisms, such as Saccharomyces cerevisiae. Previous results show that phenolic compounds and furan aldehydes, and to some extent aliphatic acids, act as inhibitors during fermentation of dilut...

journal_title：Applied biochemistry and biotechnology

authors： Nilvebrant NO,Persson P,Reimann A,De Sousa F,Gorton L,Jönsson LJ

更新日期：2003-04-01 00:00:00

abstract：:Label-free detection technique based on impedance was investigated for aflatoxin M1 (AFM1) and aflatoxin M2 (AFM2) analysis in milk products. The impedance change resulting from antigen-antibody interaction was studied using a two-electrode setup made up of silver (Ag) wire. Processed milk such as drinking yogurt and ...

journal_title：Applied biochemistry and biotechnology

authors： Kanungo L,Bacher G,Bhand S

更新日期：2014-10-01 00:00:00

abstract：:The purpose of this study was to investigate the feasibility of immobilizing Yarrowia lipolytica lipase lip2 on epoxy microspheres with or without gelatin modifications. The activity of lipase immobilized on gelatin-modified supports was twofold higher than those immobilized on native supports. There was no significan...

journal_title：Applied biochemistry and biotechnology

authors： Xie R,Cui C,Chen B,Tan T

更新日期：2015-10-01 00:00:00

abstract：:Anaerobic sequencing batch reactors containing granular or flocculent biomass have been employed successfully in the treatment of piggery wastewater. However, the studies in which these reactors were employed did not focus specifically on accelerating the hydrolysis step, even though the degradation of this chemical o...

journal_title：Applied biochemistry and biotechnology

authors： de Pinho SC,Fernandes BS,Rodrigues JA,Ratusznei SM,Foresti E,Zaiat M

更新日期：2005-02-01 00:00:00

abstract：:By sequence comparison, the majority of 1,4-α-glucan-branching enzymes (GBEs) consist of an N-terminal carbohydrate-binding domain, a TIM-barrel catalytic domain, and a C-terminal all-beta domain. Among these structures, the GBE from Geobacillus thermoglucosidans STB02 uniquely has a highly charged 26-amino-acid C-ter...

journal_title：Applied biochemistry and biotechnology

authors： Ban X,Li C,Zhang Y,Gu Z,Cheng L,Hong Y,Li Z

更新日期：2020-03-01 00:00:00

abstract：CONCLUSION:Immobilization of the thermophilic lactase causes a decrease in the formation of intermediary products. This means that partially hydrolyzed lactose syrups contain less of these intermediary products when the immobilized thermophilic lactase is used in the hydrolysis process than when a soluble lactase is us...

journal_title：Applied biochemistry and biotechnology

authors： Rugh S

更新日期：1982-01-01 00:00:00

abstract：:Atomic force microscopy was used to study the geometric structure of collagen fibrils and molecules of rat calcanean tendon tissues. The authors found that the diameter of the fibrils ranged from 124 to 170 nm, and their geometric form suggested a helical winding with spectral period from 59.4 to 61.7 nm, close to the...

journal_title：Applied biochemistry and biotechnology

authors： Baranauskas V,Vidal BC,Parizotto NA

更新日期：1998-02-01 00:00:00

abstract：:As a part of our study on microbial heterogeneity in subsurface environments, we have examined the microbial respiration of sediment samples obtained from a coastal site near Oyster, VA. The sediments at the site are unconsolidated, fine to coarse beach sand and gravel. A Columbus Instruments Micro-Oxymax Respirometer...

journal_title：Applied biochemistry and biotechnology

authors： Chapatwala KD,Babu GR,Vijaya OK,Armstead E,Palumbo AV,Zhang C,Phelps TJ

更新日期：1996-04-01 00:00:00

abstract：:The thermostable and organic solvent tolerant whole-cell lipase (WCL) was produced by Burkholderia sp. ZYB002 with broad spectrum organic solvent tolerance. The production medium of the WCL was primarily optimized, which resulted in the maximum activity of 22.8 U/mL and the 5.1-fold increase of the WCL yield. The opti...

journal_title：Applied biochemistry and biotechnology

authors： Shu ZY,Wu JG,Cheng LX,Chen D,Jiang YM,Li X,Huang JZ

更新日期：2012-02-01 00:00:00

abstract：:Nostoc microscopicum an effective extracellular polymers (EPs) synthesizer among cyanobacteria was isolated, and its elementary morphological features were defined with the aid of light microscope and CLSM. Bioseparation of EPs from 44 days-aged culture gave 0.90 g/L of the dry powdered extract. Chemical quantificatio...

journal_title：Applied biochemistry and biotechnology

authors： Ramachandran L,Marappa N,Sethumadhavan K,Nooruddin T

更新日期：2020-05-01 00:00:00

abstract：:A new cellulase producer strain of Penicillium digitatum (RV 06) was previously obtained from rotten maize grains. This work aim was to optimize the production and characterize this microorganism produced cellulase. A CMCase maximum production (1.6 U/mL) was obtained in stationary liquid culture, with an initial pH of...

journal_title：Applied biochemistry and biotechnology

authors： Dos Santos FC,de Oliveira MAS,Seixas FAV,Barbosa-Tessmann IP

更新日期：2020-09-01 00:00:00

abstract：:Cancer normally tends to result in the decrease of tissue elasticity; i.e., the cancerous region is more rigid than the normal surrounding areas. This would appear as differences in the distribution of internal birefringence that could be used to improve image contrast between the cancerous and normal tissue structure...

journal_title：Applied biochemistry and biotechnology

authors： Mathur N,Chaudhary NK,Bharti P

更新日期：2009-11-01 00:00:00

abstract：:This research investigated a novel process to prepare polyester from corn stover through liquefaction and crosslinking processes. First, corn stover was liquefied in organic solvents (90 wt% ethylene glycol and 10 wt% ethylene carbonate) with catalysts at moderate temperature under atmospheric pressure. The effect of ...

journal_title：Applied biochemistry and biotechnology

authors： Yu F,Liu Y,Pan X,Lin X,Liu C,Chen P,Ruan R

更新日期：2006-04-01 00:00:00

abstract：:Graphene-containing 13-93 bioactive glass and poly(ε-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditio...

journal_title：Applied biochemistry and biotechnology

authors： Deliormanlı AM,Atmaca H

更新日期：2018-12-01 00:00:00

abstract：:Among the technologies for heavy metal remediation, bioremediation technology has gained extensive attention because of its low processing costs and high efficiency. The white-rot fungus Phanerochaete chrysosporium (P. chrysosporium) which has a good tolerance to heavy metals has been widely used in the heavy metal bi...

journal_title：Applied biochemistry and biotechnology

authors： Wan J,Zeng G,Huang D,Huang C,Lai C,Li N,Wei Z,Xu P,He X,Lai M,He Y

更新日期：2015-02-01 00:00:00

abstract：:The use of stalks instead of tubers as a source of carbohydrates for ethanol production has been investigated. The inulin present in the stalks of Jerusalem artichoke was extracted with water and the effect of solid-liquid ratio, temperature, and acid addition was studied and optimized in order to attain a high-fructo...

journal_title：Applied biochemistry and biotechnology

authors： Negro MJ,Ballesteros I,Manzanares P,Oliva JM,Sáez F,Ballesteros M

更新日期：2006-04-01 00:00:00

abstract：:Biological control of soil-borne plant pathogens is a potential alternative to the use of chemical pesticides, which have already been proved to be harmful to the environment. Several strains of the fungus Trichoderma have been isolated and found to be effective biocontrol agents of various soil-borne plant pathogenic...

journal_title：Applied biochemistry and biotechnology

authors： Chet I,Inbar J

更新日期：1994-07-01 00:00:00

abstract：:The effect of different concentrations of zinc on the bioaccumulation of zinc and biomass yield in both mycelium and fruiting body of Pleurotus florida cultivated in liquid medium was studied. The results showed that the optimum yield of mycelia (11.33 ± 0.44 g/L) and fruiting bodies (7.70 ± 0.19 g/L) dry biomass was ...

journal_title：Applied biochemistry and biotechnology

authors： Poursaeid N,Azadbakht A,Balali GR

更新日期：2015-04-01 00:00:00