The oxidative stress of Phanerochaete chrysosporium against lead toxicity.

abstract：:Four endoglucanase temperature isoforms (T (30), T (50), T (70), and T (90)) were identified and purified from the cladodes of the xerophytic plant Opuntia vulgaris. These isoforms exhibited optimum catalytic activity at 30 °C, 50 °C, 70 °C, and 90 °C and yielded an apparent molecular mass of 150, 20, 74, and 45 kDa, ...

journal_title：Applied biochemistry and biotechnology

authors： Shyamala S,Ravikumar S,Vikramathithan J,Srikumar K

更新日期：2011-12-01 00:00:00

abstract：:Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

journal_title：Applied biochemistry and biotechnology

authors： Ferreira MS,De Andrade AV,Kennedy JF

更新日期：1991-10-01 00:00:00

abstract：:Enzymatic hydrolysis of cellulosic raw materials to produce nutrient broths for microbiological synthesis of ethanol and other valuable products is an important field of modern biotechnology. Biotechnological processing implies the selection of an effective pretreatment technique for raw materials. In this study, the ...

journal_title：Applied biochemistry and biotechnology

authors： Denisova MN,Makarova EI,Pavlov IN,Budaeva VV,Sakovich GV

更新日期：2016-03-01 00:00:00

abstract：:In this study, we investigated the dynamics of a computer simulation of a continuous alcoholic fermentation process combined with a flash column under vacuum. The alcohol was partially extracted in order to maintain its concentration at about 40 kg/m3 in the fermentor. The mathematical model of the fermentation was de...

journal_title：Applied biochemistry and biotechnology

authors： Costa AC,Dechechi EC,Silva FL,Maugeri F,Maciel R

更新日期：2000-04-01 00:00:00

abstract：:The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration...

journal_title：Applied biochemistry and biotechnology

authors： Zhou Y,Domínguez JM,Cao N,Du J,Tsao GT

更新日期：1999-04-01 00:00:00

abstract：:Foam fractionation is one of the low operating-cost techniques for removing proteins from a dilute solution. The initial bulk solution pH and air superficial velocity play an important role in the foam-fractionation process. Denaturation of proteins (enzymes) can occur, however, during the foam-fractionation process f...

journal_title：Applied biochemistry and biotechnology

authors： Loha V,Prokop A,Du L,Tanner RD

更新日期：1999-04-01 00:00:00

abstract：:Based on the p426 series of expression vectors developed by Mumberg et al. (Gene 156, 119-122, 1995), we have generated a set of plasmids that allow the glucose-dependent expression of target genes in the yeast, Saccharomyces cerevisiae. The ADH1 promoter in plasmid p426-ADH1 was replaced by the 1-kb 5'-region from ei...

journal_title：Applied biochemistry and biotechnology

authors： Thierfelder S,Ostermann K,Göbel A,Rödel G

更新日期：2011-04-01 00:00:00

abstract：:Methods for isothermal amplification of nucleic acids are gained more attention in the last two decades. For isothermal amplification, DNA polymerases with strand displacement activity are required, and Bst exo- is one of the most commonly used polymerases. However, Bst exo- is able to cause nonspecific DNA amplificat...

journal_title：Applied biochemistry and biotechnology

authors： Garafutdinov RR,Gilvanov AR,Sakhabutdinova AR

更新日期：2020-02-01 00:00:00

abstract：:When dilute-acid hydrolysates from spruce are fermented to produce ethanol, detoxification is required to make the hydrolysates fermentable at reasonable rates. Treatment with alkali, usually by overliming, is one of the most efficient approaches. Several nutrients, such as ammonium and phosphate, are added to the hyd...

journal_title：Applied biochemistry and biotechnology

authors： Alriksson B,Horváth IS,Sjöde A,Nilvebrant NO,Jönsson LJ

更新日期：2005-04-01 00:00:00

abstract：:Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Marques M,Machoshvili IA,Ishii M

更新日期：2002-04-01 00:00:00

abstract：:This study was carried out to evaluate the capacity of anaerobic granular sludge for oxidizing ammonium and p-cresol with nitrate as terminal electron acceptor. Kinetics for the anaerobic oxidation of ammonium and p-cresol is described in this paper. The phenolic compound was very efficiently consumed, achieving 65 % ...

journal_title：Applied biochemistry and biotechnology

authors： González-Blanco G,Cervantes FJ,Beristain-Cardoso R,Gómez J

更新日期：2015-08-01 00:00:00

abstract：:Amyloglucosidase from Novo (Copenhagen, Denmark) was immobilized in controlled pore silica particles with the silane-glutaraldehyde covalent method. Thermal stability of the free and immobilized enzyme (IE) was determined with 30% (w/v) alpha-amylase liquefied cassava starch, pH 4.5, temperatures from 35 to 75 degrees...

journal_title：Applied biochemistry and biotechnology

authors： Zanin GM,De Moraes FF

更新日期：1998-04-01 00:00:00

abstract：:A biosensor for ascorbic acid based on enzyme kinetics of ascorbate oxidase (E.C.1.10.3.3) was developed. The enzyme was extracted from Cucurbita maxima, or jerimun and immobilized by covalent bounding, using glutaradehyde as a bifunctional agent, on alkylamine glass beads, with and without enzyme active site protecti...

journal_title：Applied biochemistry and biotechnology

authors： Marques ET Jr,Lima Filho JL

更新日期：1992-01-01 00:00:00

abstract：:Label-free detection technique based on impedance was investigated for aflatoxin M1 (AFM1) and aflatoxin M2 (AFM2) analysis in milk products. The impedance change resulting from antigen-antibody interaction was studied using a two-electrode setup made up of silver (Ag) wire. Processed milk such as drinking yogurt and ...

journal_title：Applied biochemistry and biotechnology

authors： Kanungo L,Bacher G,Bhand S

更新日期：2014-10-01 00:00:00

abstract：:Maltodextrins and hydrolysates of rice and corn flour of varying dextrose equivalents (DE) have been used as a carbon source for alpha-amylase enzyme production by Bacillus sp. TCRDC-25A. The rate and total enzyme production was higher in maltodextrin media than in cornstarch. The enzyme production increased with incr...

journal_title：Applied biochemistry and biotechnology

authors： Bajpai P,Bajpai PK

更新日期：1991-11-01 00:00:00

abstract：:This study aimed to evaluate the influence of the culture medium supplementation with mineral ions, focusing on the growth of Streptococcus zooepidemicus as well as on the production and average molecular weight (MW) of hyaluronic acid (HA). The ions were investigated in terms of individual absence from the totally su...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Eguchi SY,Santana MH

更新日期：2010-12-01 00:00:00

abstract：:Convenient expression systems for efficient heterologous production of different laccases are needed for their characterization and application. The laccase cDNAs lcc1 and lcc2 from Trametes versicolor were expressed in Pichia pastoris and Aspergillus niger under control of their respective glyceraldehyde-3-phosphate ...

journal_title：Applied biochemistry and biotechnology

authors： Bohlin C,Jönsson LJ,Roth R,van Zyl WH

更新日期：2006-04-01 00:00:00

abstract：:1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...

journal_title：Applied biochemistry and biotechnology

authors： Pornwongthong P,Mulchandani A,Gedalanga PB,Mahendra S

更新日期：2014-05-01 00:00:00

abstract：:The use of cheap, high-efficiency, and ecofriendly adsorbent has been studied as an alternative way for the removal of dyes from wastewater. This paper investigated the use of waste brewery's yeast for the removal of acid orange 7 from aqueous solution. The optimum removal of acid orange 7 was found to be 3.561 mg/g a...

journal_title：Applied biochemistry and biotechnology

authors： Wu Y,Hu Y,Xie Z,Feng S,Li B,Mi X

更新日期：2011-04-01 00:00:00

abstract：:Glycerol metabolism is a typical biological oxidoreductive reaction. 1,3-Propanediol (1,3-PD) is the final product of the reductive branch, while acetate, succinate, lactate, 2,3-butanediol (2,3-BD), and ethanol were produced in the oxidative branch. 2,3-BD, which has similar properties of high boiling point and water...

journal_title：Applied biochemistry and biotechnology

authors： Zhang G,Yang G,Wang X,Guo Q,Li Y,Li J

更新日期：2012-09-01 00:00:00

abstract：:Lipase from Rhizomucor miehei (RML) was immobilized onto chitosan support in the presence of some surfactants added at low levels using two different strategies. In the first approach, the enzyme was immobilized in the presence of surfactants on chitosan supports previously functionalized with glutaraldehyde. In the s...

journal_title：Applied biochemistry and biotechnology

authors： de Oliveira UMF,Lima de Matos LJB,de Souza MCM,Pinheiro BB,Dos Santos JCS,Gonçalves LRB

更新日期：2018-04-01 00:00:00

abstract：:Proinflammatory cytokines such as tumor necrosis factor (TNF) alpha are well known to inhibit adipocyte differentiation. TNF-alpha triggers ceramide synthesis through binding of TNF-alpha to its p55 receptor. Therefore, ceramide is implicated in many of the multiple signaling pathways initiated by TNF-alpha. In breast...

journal_title：Applied biochemistry and biotechnology

authors： Xu F,Yang CC,Gomillion C,Burg KJ

更新日期：2010-01-01 00:00:00

abstract：:Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a highly sensitive, simple, and rapid method for analysis of bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies in vacci...

journal_title：Applied biochemistry and biotechnology

authors： Xue P,Zhang K,Zhang Z,Li Y,Liu F,Sun Y,Zhang X,Song C,Fu A,Jin B,Yang K

更新日期：2012-03-01 00:00:00

abstract：:During the course of fermentation, online measuring procedures able to estimate the performance of the current operation are highly desired. Unfortunately, the poor mechanistic understanding of most biologic systems hampers attempts at direct online evaluation of the bioprocess, which is further complicated by the lac...

journal_title：Applied biochemistry and biotechnology

authors： Bicciato S,Bagno A,Soldà M,Manfredini R,Di Bello C

更新日期：2002-07-01 00:00:00

abstract：:Culture fluorescence measurement technique has the potential for on-line characterization of metabolic status of fermentation processes. Many fluorophores present inside the living cells such as NADH + H+, tryptophan, pyridoxine, and riboflavin fluoresce at specific excitation and emission wavelength combinations. Sin...

journal_title：Applied biochemistry and biotechnology

authors： Khanna S,Srivastava AK

更新日期：2009-05-01 00:00:00

abstract：:The articular cartilage is an avascular and aneural tissue and its injuries result mostly in osteoarthritic changes and formation of fibrous tissue. Efforts of scientists worldwide are focused on restoration of cartilage with increase in life quality of patients. Novel polymeric polyhydroxybutyrate/chitosan (PCH) poro...

journal_title：Applied biochemistry and biotechnology

authors： Giretova M,Medvecky L,Petrovova E,Cizkova D,Danko J,Mudronova D,Slovinska L,Bures R

更新日期：2019-10-01 00:00:00

abstract：:The bacteriocins of lactic acid bacteria have considerable potential for biopreservation. The Lactococcus lactis strain PSY2 (GenBank account no. JF703669) isolated from the surface of marine perch Perca flavescens produced antibacterial activity against pathogenic and spoilage-causing Gram-positive and Gram-negative ...

journal_title：Applied biochemistry and biotechnology

authors： Sarika AR,Lipton AP,Aishwarya MS,Dhivya RS

更新日期：2012-07-01 00:00:00

abstract：:The first step involved in the degradation of phthalate isomers (phthalate, isophthalate and terephthalate) is the double hydroxylation by respective aromatic-ring hydroxylating dioxygenases. These are two component enzymes consisting of 'oxygenase' and 'reductase' components. Soil isolate Pseudomonas aeruginosa strai...

journal_title：Applied biochemistry and biotechnology

authors： Karandikar R,Badri A,Phale PS

更新日期：2015-09-01 00:00:00

abstract：:In a recombinant antibody scFv format, the presence of an unpaired cysteine (Cys) is implicated in reduced soluble expression and inefficient presentation in phage display. Compared to other species, antibodies derived from rabbits are more likely to contain this unpaired Cys residue at position 80 (Cys80), when gener...

journal_title：Applied biochemistry and biotechnology

authors： Ayyar BV,Hearty S,O'Kennedy R

更新日期：2018-07-01 00:00:00

abstract：:Xanthomonas campestris w.t. was used for production of xanthan gum in fermentations with chestnut flour for the first time. Fermentations were carried out with either chestnut flour or its soluble sugars (33.5%) and starch (53.6%), respectively, at 28 degrees C and 200 rpm at initial pH 7.0 in flasks. The effect of ag...

journal_title：Applied biochemistry and biotechnology

authors： Liakopoulou-Kyriakides M,Psomas SK,Kyriakidis DA

更新日期：1999-12-01 00:00:00