Liquefaction of corn stover and preparation of polyester from the liquefied polyol.

abstract：:1,4-Dioxane, a contaminant increasingly detected in water supplies, is a public health concern because it is classified as a possible human carcinogen. 1,4-Dioxane can be biodegraded by aerobic bacteria via monooxygenase-catalyzed reactions. While these metalloenzymes require trace metals as cofactors in their catalyt...

journal_title：Applied biochemistry and biotechnology

authors： Pornwongthong P,Mulchandani A,Gedalanga PB,Mahendra S

更新日期：2014-05-01 00:00:00

abstract：:L-asparaginase production was optimized using isolated Bacillus circulans (MTCC 8574) under solid-state fermentation (SSF) using locally available agricultural waste materials. Among different agricultural materials (red gram husk, bengal gram husk, coconut, and groundnut cake), red gram husk gave the maximum enzyme p...

journal_title：Applied biochemistry and biotechnology

authors： Hymavathi M,Sathish T,Subba Rao Ch,Prakasham RS

更新日期：2009-10-01 00:00:00

abstract：:The objective of this work was to take advantage of the resting cells of suitable fungus as an in vitro model to prepare gastrodin from p-2-hydroxybenzyl alcohol (HBA), which mainly exists in the metabolites of the plant Gastrodia elata Blume. The one-step biotransformation of HBA into gastrodin was examined with the ...

journal_title：Applied biochemistry and biotechnology

authors： Fan L,Dong Y,Xu T,Zhang H,Chen Q

更新日期：2013-05-01 00:00:00

abstract：:The recombinant Escherichia coli gamma-glutamyltranspeptidase (EcGGT) was immobilized in Ca-alginate-kappa-carrageenan beads. Effects of alginate concentration, amount of loading enzyme, and bead size on the entrapped activity were investigated. Optimum alginate concentration for EcGGT immobilization was found to be 2...

journal_title：Applied biochemistry and biotechnology

authors： Hung CP,Lo HF,Hsu WH,Chen SC,Lin LL

更新日期：2008-08-01 00:00:00

abstract：:For trial use in the local chemotherapy of cancer by a combination of cytosine deaminase (EC 3.5.4.1) and 5-fluorocytosine (J. Biotechnol., (1985), 2, 13-21), 40 U of partially purified cytosine deaminase was obtained from 500 g of commercial compressed bakers' yeast. The enzyme, which is unstable, was immobilized to ...

journal_title：Applied biochemistry and biotechnology

authors： Katsuragi T,Sakai T,Tonomura K

更新日期：1987-09-01 00:00:00

abstract：:The common view of amyloids and prion proteins is that they are associated with many currently incurable diseases and present a great danger to an organism. This danger comes from the fact that not only prion proteins, but also the infectious form(s) of amyloids, as it has been shown recently, are able to transmit the...

journal_title：Applied biochemistry and biotechnology

authors： Pulawski W,Ghoshdastider U,Andrisano V,Filipek S

更新日期：2012-04-01 00:00:00

abstract：:An extracellular lipase was purified from the fermentation broth of Penicillium expansum PED-03 by DEAE-Sepharose chromatography, followed by sephacryl S-200 chromatography. The enzyme was purified 81.8-fold with 19.8% recovery and a specific activity of 85.94 U/mg. The molecular weight of the homogeneous enzyme was a...

journal_title：Applied biochemistry and biotechnology

authors： Lianghua T,Liming X,Min S,Huaying G

更新日期：2007-08-01 00:00:00

abstract：:The high cost of nicotinamide cofactors requires that they be regenerated in situ when used in preparative enzymatic synthesis. Numerous strategies have been tested for in situ regeneration of reduced and oxidized cofactors. Regeneration of reduced cofactors is relatively straightforward; regeneration of oxidized cofa...

journal_title：Applied biochemistry and biotechnology

authors： Chenault HK,Whitesides GM

更新日期：1987-03-01 00:00:00

abstract：:Termitomyces fungi associated with fungus-growing termites are the edible mushrooms and can produce useful chemicals, enzymes, and volatile organic compounds (VOCs) that have both fuel and biological potentials. To this purpose, we examined the Termitomyces mycelial growth performance on various substrates, clarified ...

journal_title：Applied biochemistry and biotechnology

authors： Yang G,Ahmad F,Liang S,Fouad H,Guo M,Gaal HA,Mo J

更新日期：2020-12-01 00:00:00

abstract：:One-stage autoheated thermophilic aerobic digestion (ATAD) can stabilize sludge to meet class A standard. In this study, batch experiments were conducted to investigate the characteristics of oxidation-reduction potential (ORP), volatile fatty acids (VFAs), soluble chemical oxygen demand (SCOD), and nutrients at diffe...

journal_title：Applied biochemistry and biotechnology

authors： Cheng J,Kong F,Zhu J,Wu X

更新日期：2015-01-01 00:00:00

abstract：:Volatile C6-aldehydes are the main contributors to the characteristic odor of plants known as "green note" and are widely used by the flavor industry. Biotechnological processes were developed to fulfill the high demand in C6-aldehydes in natural flavorants and odorants. Recombinant hydroperoxide lyases (HPLs) constit...

journal_title：Applied biochemistry and biotechnology

authors： Jacopini S,Mariani M,de Caraffa VB,Gambotti C,Vincenti S,Desjobert JM,Muselli A,Costa J,Berti L,Maury J

更新日期：2016-06-01 00:00:00

abstract：:Extractive fermentation is a widely preferred technique in which the products of fermentation are removed from the fermentation medium by a proper solvent, in order to avoid the inhibitory effects of the products. In this work, decanol, which has a high distribution coefficient with respect to the biocompatible solven...

journal_title：Applied biochemistry and biotechnology

authors： Kapucu H,Mehmetoğlu U

更新日期：1998-11-01 00:00:00

abstract：:In biotechnological processes, a great number of factors can influence the income productivity and conversion. Normally, it is not evident which of these factors are the most important and how they interact. In this work, multivariate analysis techniques are used as experimental design coupled to a detailed determinis...

journal_title：Applied biochemistry and biotechnology

authors： Lunelli BH,Rivera EC,Vasco de Toledo EC,Wolf Maciel MR,Maciel Filho R

更新日期：2008-03-01 00:00:00

abstract：:Glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase is an enzyme that converts GL-7-ACA to 7-aminocephalosporanic acid, a starting material for semisynthetic cephalosporin antibiotics. In this study, optimal conditions for the immobilization of GL-7-ACA acylase were determined by experimental observations and stat...

journal_title：Applied biochemistry and biotechnology

authors： Park SW,Lee JW,Hong SI,Kim SW

更新日期：2003-03-01 00:00:00

abstract：:The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 6...

journal_title：Applied biochemistry and biotechnology

authors： Errasti ME,Natalucci CL,Caffini NO,Rotelli AE,Brullo A,Maras B,Trejo SA,López LMI

更新日期：2018-09-01 00:00:00

abstract：:The increased additive amount of adjuncts in the raw materials of Chinese beer requires the usage of protease to release more water-soluble proteins. Here, a metallo-neutral protease suited for brewing industry was purified from Bacillus amyloliquefaciens SYB-001. A 5.6-fold purification of the neutral protease was ac...

journal_title：Applied biochemistry and biotechnology

authors： Wang J,Xu A,Wan Y,Li Q

更新日期：2013-08-01 00:00:00

abstract：:An aspartic protease gene from Pleurotus ostreatus (Po-Asp) had been cloned based on the 3' portion of cDNA in our previous work. The Po-Asp cDNA contained 1,324 nucleotides with an open reading frame (ORF) of 1,212 bp encoding 403 amino acid residues. The putative amino acid sequence included a signal peptide, an act...

journal_title：Applied biochemistry and biotechnology

authors： Yin C,Zheng L,Chen L,Tan Q,Shang X,Ma A

更新日期：2014-02-01 00:00:00

abstract：:The enzymatic hydrolysates of the lipid-extracted microalgal biomass residues (LMBRs) from biodiesel production were evaluated as nutritional sources for the mixotrophic growth of Chlorella vulgaris and lipid production at different temperature levels and substrate concentrations. Both parameters had a significant eff...

journal_title：Applied biochemistry and biotechnology

authors： Ma X,Zheng H,Huang H,Liu Y,Ruan R

更新日期：2014-10-01 00:00:00

abstract：:Flavonoids are secondary metabolites synthesized by plants shown to exhibit health benefits such as anti-inflammatory, antioxidant, and anti-tumor effects. Thus, due to the importance of this compound, several enzymes involved in the flavonoid pathway have been cloned and characterized in Escherichia coli. However, th...

journal_title：Applied biochemistry and biotechnology

authors： Zakaria II,Rahman RN,Salleh AB,Basri M

更新日期：2011-09-01 00:00:00

abstract：:In a previous study, a recombination system based on the alpha complementation of cre recombinase and protein transduction was established. This system relied on the transient expression of the inactive, self-excisable C-terminal (beta) and the transduction of the N-terminal (alpha) cre fragments to cells as a purifie...

journal_title：Applied biochemistry and biotechnology

authors： Seidi A,Mie M,Kobatake E

更新日期：2009-08-01 00:00:00

abstract：:This study aimed to evaluate the influence of the culture medium supplementation with mineral ions, focusing on the growth of Streptococcus zooepidemicus as well as on the production and average molecular weight (MW) of hyaluronic acid (HA). The ions were investigated in terms of individual absence from the totally su...

journal_title：Applied biochemistry and biotechnology

authors： Pires AM,Eguchi SY,Santana MH

更新日期：2010-12-01 00:00:00

abstract：:An improved procedure has been developed for efficient somatic embryogenesis in Anethum graveolens. Green friable embryogenic callus was obtained from hypocotyl segments on medium augmented with 2,4-dichlorophenoxyacetic acid (2,4-D). The highest embryogenic callus induction frequency of 87 % was obtained on Murashige...

journal_title：Applied biochemistry and biotechnology

authors： Dhir R,Shekhawat GS,Alam A

更新日期：2014-08-01 00:00:00

abstract：:Thermostable invertase (E.C. 3.2.1.26) and inulinase 2,1-beta-D-fructan fructanohydrolase (E.C. 3.2.1.7) activities were produced by Cladosporium cladosporioides grown on sucrose, inulin, yam extract, or Jerusalem artichoke. The ratio I (inulinase)/S(invertase) activity was between 0.31 and 0.36. Both activities had h...

journal_title：Applied biochemistry and biotechnology

authors： Ferreira MS,De Andrade AV,Kennedy JF

更新日期：1991-10-01 00:00:00

abstract：:The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of ...

journal_title：Applied biochemistry and biotechnology

authors： Abi A,Wang A,Jördening HJ

更新日期：2018-12-01 00:00:00

abstract：:Vacuolar processing enzymes (VPEs) have received considerable attention recently, as they exhibit caspase-1-like cleavage activity and regulate the process of PCD. However, knowledge about their detailed characteristics and structures is relatively limited. In this study, a gamma vacuolar processing enzyme gene, MhVPE...

journal_title：Applied biochemistry and biotechnology

authors： Ran K,Yang H,Sun X,Li Q,Jiang Q,Zhang W,Shen W

更新日期：2014-05-01 00:00:00

abstract：:Oil palm empty fruit bunch (EFB) is abundantly available in Malaysia and it is a potential source of xylose for the production of high-value added products. This study aimed to optimize the hydrolysis of EFB using dilute sulfuric acid (H2SO4) and phosphoric acid (H3PO4) via response surface methodology for maximum xyl...

journal_title：Applied biochemistry and biotechnology

authors： Tan HT,Dykes GA,Wu TY,Siow LF

更新日期：2013-08-01 00:00:00

abstract：:Degradation of known odor precursors in sludge during anaerobic digestion was systematically studied and simulated using the Anaerobic Digestion Model Number 1 (ADM1). The degradation of various protein fractions (particulate, soluble, and bound), volatile fatty acids (VFAs), lipids, and amino acids of primary sludge ...

journal_title：Applied biochemistry and biotechnology

authors： Aldin S,Tu F,Nakhla G,Ray MB

更新日期：2011-08-01 00:00:00

abstract：:Different initial cell concentrations of a recombinant flocculent Saccharomyces cerevisiae MA-R4 were evaluated for their effects on xylose fermentation and glucose-xylose cofermentation. A high initial cell concentration greatly increased both the substrate utilization and ethanol production rates. During xylose ferm...

journal_title：Applied biochemistry and biotechnology

authors： Matsushika A,Sawayama S

更新日期：2010-11-01 00:00:00

abstract：:To meet the increasing demands of sophorolipids as biosurfactants and bioactive compounds, it is necessary to obtain higher and more specific sophorolipid-producing strains. One sophorolipid-producing strain, Wickerhamiella domercqiae var. sophorolipid CGMCC 1576 (Y(2A)), was mutated by low-energy nitrogen ion beam im...

journal_title：Applied biochemistry and biotechnology

authors： Li H,Ma X,Shao L,Shen J,Song X

更新日期：2012-06-01 00:00:00

abstract：:An extracellular polygalacturonase (PG) produced from Paecilomyces variotii was purified to homogeneity through two chromatography steps using DEAE-Fractogel and Sephadex G-100. The molecular weight of P. variotii PG was 77,300 Da by gel filtration and SDS-PAGE. PG had isoelectric point of 4.37 and optimum pH 4.0. PG ...

journal_title：Applied biochemistry and biotechnology

authors： de Lima Damásio AR,da Silva TM,Maller A,Jorge JA,Terenzi HF,Polizeli Mde L

更新日期：2010-03-01 00:00:00