Gastrodin production from p-2-hydroxybenzyl alcohol through biotransformation by cultured cells of Aspergillus foetidus and Penicillium cyclopium.


:The objective of this work was to take advantage of the resting cells of suitable fungus as an in vitro model to prepare gastrodin from p-2-hydroxybenzyl alcohol (HBA), which mainly exists in the metabolites of the plant Gastrodia elata Blume. The one-step biotransformation of HBA into gastrodin was examined with the filamentous fungi cells of Aspergillus foetidus and Penicillium cyclopium AS 3.4513 in this study. The fundamental conditions of biotransformation were screened and compared for both fungi. P. cyclopium AS 3.4513 had better gastrodin-producing capability than A. foetidus through one-step bioconversion. The highest yield of gastrodin was 36 mg/L for A. foetidus ZU-G1 and 65 mg/L for P. cyclopium AS 3.4513 under the respective development condition during 6 days of biotransformation. The comparative results show that P. cyclopium AS 3.4513 reveals great potential to form gastrodin using HBA as the precursor. The products catalyzed by the resting cells of P. cyclopium AS 3.4513 were identified through NMR and ESI-MS. Current results can be applied not only to the chemical synthesis processes that may involve the hydroxylation reaction but also to the industrial production. The selected fungus is the potential biocatalyst for HBA glucosylation.


Fan L,Dong Y,Xu T,Zhang H,Chen Q




Has Abstract


2013-05-01 00:00:00












  • Preserving the activity of cellulase in a batch foam fractionation process.

    abstract::Foam fractionation is one of the low operating-cost techniques for removing proteins from a dilute solution. The initial bulk solution pH and air superficial velocity play an important role in the foam-fractionation process. Denaturation of proteins (enzymes) can occur, however, during the foam-fractionation process f...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Loha V,Prokop A,Du L,Tanner RD

    更新日期:1999-04-01 00:00:00

  • Separation and immobilization of lipase from Penicillium simplicissimum by selective adsorption on hydrophobic supports.

    abstract::Lipases are an enzyme class of a great importance as biocatalysts applied to organic chemistry. However, it is still necessary to search for new enzymes with special characteristics such as good stability towards high temperatures, organic solvents, and high stereoselectivity presence. The present work's aim was to im...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Cunha AG,Fernández-Lorente G,Gutarra ML,Bevilaqua JV,Almeida RV,Paiva LM,Fernández-Lafuente R,Guisán JM,Freire DM

    更新日期:2009-05-01 00:00:00

  • Mobilization and biodegradation of 2-methylnaphthalene by amphiphilic polyurethane nano-particle.

    abstract::Amphiphilic polyurethane (APU) nano-particle enhanced the mobilization of 2-methylnaphthalene (2-MNPT) in soil. Significant increase in the solubility of 2-MNPT was achieved. The molar solubilization ratio was 0.4 (mole 2-MNPT/mole APU). Simple precipitation of APU particle by 2 N CaCl(2) recovered 95% of APU particle...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kim YB,Kim JY,Kim EK

    更新日期:2009-10-01 00:00:00

  • Co-expression of recombinant nucleoside phosphorylase from Escherichia coli and its application.

    abstract::The genes encoding purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase), and thymidine phosphorylase (TPase) from Escherichia coli K12 were cloned respectively into expression vector pET-11a or pET-28a. The recombinant plasmids were transformed into the host strain E. coli BL21(DE3) to construct fou...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Ge C,OuYang L,Ding Q,Ou L

    更新日期:2009-10-01 00:00:00

  • Isolation of an organic solvent-tolerant lipolytic enzyme from uncultivated microorganism.

    abstract::Although the use of lipases as biocatalysts has frequently been proposed, it is yet scarcely being implemented in industrial processes. This is mainly due to the difficulties associated with the discovery and engineering of new enzymes and the lack of versatile screening methods. In this study, we screened the availab...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Roh C,Schmid RD

    更新日期:2013-12-01 00:00:00

  • Axenic cultures of Nitrosomonas europaea and Nitrobacter winogradskyi in autotrophic conditions: a new protocol for kinetic studies.

    abstract::As a part of a natural biological N-cycle, nitrification is one of the steps included in the conception of artificial ecosystems designed for extraterrestrial life support systems (LSS) such as Micro-Ecological Life Support System Alternative (MELiSSA) project, which is the LSS project of the European Space Agency. Ni...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Farges B,Poughon L,Roriz D,Creuly C,Dussap CG,Lasseur C

    更新日期:2012-07-01 00:00:00

  • A Biotechnological Strategy for Molybdenum Extraction Using Acidithiobacillus ferrooxidans.

    abstract::Biosorption is a potential tool for the extraction of metals from contaminated water and recovery of precious metals, which is a convenient alternative to conventional processes. In the present study, molybdenum recovery by Acidithiobacillus ferrooxidans strain ZT-94 was evaluated. Additionally, the effects of pH init...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Kasra-Kermanshahi R,Tajer-Mohammad-Ghazvini P,Bahrami-Bavani M

    更新日期:2020-11-17 00:00:00

  • Pyrolysis oil-based lipid production as biodiesel feedstock by Rhodococcus opacus.

    abstract::Light oil from pyrolysis, which accounts for ∼10 % carbon yield of the starting biomass, is a complex aqueous product that is difficult to utilize and usually discarded. This work presents the feasibility of light oil as a sole carbon source to support the growth of Rhodococcus opacus (R. opacus) that in turn accumula...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Wei Z,Zeng G,Kosa M,Huang D,Ragauskas AJ

    更新日期:2015-01-01 00:00:00

  • Preferential lectin binding of cancer cells upon sialic acid treatment under nutrient deprivation.

    abstract::The terminal monosaccharide of glycoconjugates on a eukaryotic cell surface is typically a sialic acid (Neu5Ac). Increased sialylation usually indicates progression and poor prognosis of most carcinomas. Here, we utilize two human mammary epithelial cell lines, HB4A (breast normal cells) and T47D (breast cancer cells)...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Badr HA,Elsayed AI,Ahmed H,Dwek MV,Li CZ,Djansugurova LB

    更新日期:2013-10-01 00:00:00

  • 6-Tosyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide analogues: synthesis, characterization, MO calculation, and antibacterial activity.

    abstract::A series of 6-tosyl-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide analogues are synthesized by conventional techniques and characterized by elemental analysis, IR, MS, (1)H, and (13)C NMR. These are tested for their antibacterial activity against Bacillus subtilis (abbreviated as BS), Staphylococcus aureus (ab...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Doshi H,Thakkar S,Khirsariya P,Thakur MC,Ray A

    更新日期:2015-02-01 00:00:00

  • High-yield hypocrellin A production in solid-state fermentation by Shiraia sp. SUPER-H168.

    abstract::Hypocrellin A production by Shiraia sp. SUPER-H168 was studied under solid-state fermentation. Corn was found to be the best substrate after evaluating eight kinds of agro-industrial crops and residues. The optimized solid-state fermentation conditions were as follows: inoculum size 3 x 10(6) spores, substrate particl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Cai Y,Liang X,Liao X,Ding Y,Sun J,Li X

    更新日期:2010-04-01 00:00:00

  • Cofermentation of glucose, xylose, and arabinose by genomic DNA-integrated xylose/arabinose fermenting strain of Zymomonas mobilis AX101.

    abstract::Cofermentation of glucose, xylose, and arabinose is critical for complete bioconversion of lignocellulosic biomass, such as agricultural residues and herbaceous energy crops, to ethanol. We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, a...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Mohagheghi A,Evans K,Chou YC,Zhang M

    更新日期:2002-04-01 00:00:00

  • Biomethane production in an AnSBBR treating wastewater from biohydrogen process.

    abstract::An anaerobic sequencing batch reactor containing immobilized biomass (AnSBBR) was used to produce biomethane by treating the effluent from another AnSBBR used to produce biohydrogen from glucose- (AR-EPHG) and sucrose-based (AR-EPHS) wastewater. In addition, biomethane was also produced from sucrose-based synthetic wa...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Lullio TG,Souza LP,Ratusznei SM,Rodrigues JA,Zaiat M

    更新日期:2014-11-01 00:00:00

  • Optimization of L-lactic acid production from glucose by Rhizopus oryzae ATCC 52311.

    abstract::The effect of nutrients on L(+)-lactic acid production from glucose was investigated using Rhizopus oryzae ATCC 52311. From the shake-flask experiments, the optimal medium composition was defined for improved lactic-acid production. In order to enhance lactic-acid production rate and product yield, controlled aeration...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhou Y,Domínguez JM,Cao N,Du J,Tsao GT

    更新日期:1999-04-01 00:00:00

  • Production of biodiesel via enzymatic ethanolysis of the sunflower and soybean oils: modeling.

    abstract::Biodiesel has become attractive due to its environmental benefits compared with conventional diesel. Although the enzymatic synthesis of biodiesel requires low thermal energy, low conversions of enzymatic transesterification with ethanol (ethanolysis) of oils to produce biodiesel are reported as a result of deactivati...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Pessoa FL,Magalhães SP,Falcão PW

    更新日期:2010-05-01 00:00:00

  • Sphaerotilus natans isolated from activated sludge and its production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate).

    abstract::Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Liu K,Chua H,Lo WH,Lawford H,Yu PH

    更新日期:2002-04-01 00:00:00

  • Rapid Formation of Aerobic Granular Sludge and Its Mechanism in a Continuous-Flow Bioreactor.

    abstract::Based on the principle of self-coagulation of microorganisms, the flocculant-producing denitrifying bacterial TN-14 sludge was added to the continuous-flow reactor for treating domestic sewage. The bacterial TN-14 sludge acted as the main seed sludge to promote the rapid formation of aerobic granular sludge. The sludg...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Xin X,Lu H,Yao L,Leng L,Guan L

    更新日期:2017-01-01 00:00:00

  • Biotechnological and molecular approaches for vanillin production: a review.

    abstract::Vanillin is one of the most widely used flavoring agents in the world. As the annual world market demand of vanillin could not be met by natural extraction, chemical synthesis, or tissue culture technology, thus biotechnological approaches may be replacement routes to make production of bio-vanillin economically viabl...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章,评审


    authors: Kaur B,Chakraborty D

    更新日期:2013-02-01 00:00:00

  • Triggering of the antibacterial activity of Bacillus subtilis B38 strain against methicillin-resistant Staphylococcus aureus.

    abstract::When cultured in minimal growth medium, the B38 strain of Bacillus subtilis did not exhibit any antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolate. Coculturing B38 strain with viable MRSA cells weakly increased antibacterial activity production (20 AU/ml). Addition of de...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Tabbene O,Karkouch I,Slimene IB,Elfeddy N,Cosette P,Mangoni ML,Jouenne T,Limam F

    更新日期:2011-05-01 00:00:00

  • Structural Properties of Macrodontain I, a Cysteine Protease from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae).

    abstract::The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 6...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Errasti ME,Natalucci CL,Caffini NO,Rotelli AE,Brullo A,Maras B,Trejo SA,López LMI

    更新日期:2018-09-01 00:00:00

  • Purification, characterization, and molecular cloning of a novel antifungal lectin from the roots of Ophioglossum pedunculosum.

    abstract::A novel mannan-specific lectin was isolated from the roots of a traditional Chinese herbal medicine, Ophioglossum pedunculosum through ion-exchange chromatography and gel filtration. With a molecular mass of 19,835.7 Da demonstrated by MALDI-TOF analysis, this novel agglutinin was designated as O. pedunculosum aggluti...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: He XM,Ji N,Xiang XC,Luo P,Bao JK

    更新日期:2011-12-01 00:00:00

  • Reusability of entrapped cells of Pseudomonas diminuta for production of 7-aminocephalosporanic acid.

    abstract::Entrapped cells of P. diminuta were used for the production of 7-aminocephalosporanic acid (7-ACA), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. The repeated batch production of 7-ACA with entrapped cells of P. diminuta in ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Nigam VK,Kundu S,Ghosh P

    更新日期:2007-04-01 00:00:00

  • Elicitation of andrographolide in the suspension cultures of Andrographis paniculata.

    abstract::Andrographis paniculata belonging to the family Acanthaceae produces a group of diterpene lactones, one of which is the pharmaceutically important-andrographolide. It is known to possess various important biological properties like anticancer, anti-HIV, anti-inflammatory, etc. This is the first report on the productio...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Gandi S,Rao K,Chodisetti B,Giri A

    更新日期:2012-12-01 00:00:00

  • Effect of ceramide on mesenchymal stem cell differentiation toward adipocytes.

    abstract::Proinflammatory cytokines such as tumor necrosis factor (TNF) alpha are well known to inhibit adipocyte differentiation. TNF-alpha triggers ceramide synthesis through binding of TNF-alpha to its p55 receptor. Therefore, ceramide is implicated in many of the multiple signaling pathways initiated by TNF-alpha. In breast...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Xu F,Yang CC,Gomillion C,Burg KJ

    更新日期:2010-01-01 00:00:00

  • Redesign the α/β fold to enhance the stability of mannanase Man23 from Bacillus subtilis.

    abstract::In this work, we engineered the α/β fold of mannanase Man23 based on its molecular structure analysis to obtain more stable variants. By introducing 31 single-site mutations in the α/β fold and shuffling them, the incorporation of four mutations (K178R, K207R, N340R, and S354R) displayed a good balance between high ac...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Zhou HY,Pan HY,Rao LQ,Wu YY

    更新日期:2011-01-01 00:00:00

  • Cloning, overexpression, purification, and characterization of receptor-interacting protein 3 truncation in Escherichia coli.

    abstract::To facilitate structural studies of receptor-interacting protein 3 (RIP3), we developed a large-scale expression system of a glutathione-S-transferase (GST) fused with an 82 amino acid RIP3 protein in Escherichia coli. RIP3 truncation was subcloned into the pGEX-4T-1 vector and overexpressed in BL21(DE3)RIL cells. The...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Jeong MS,Park JS,Jang SB

    更新日期:2007-05-01 00:00:00

  • Understanding the Impacts of AFEX™ Pretreatment and Densification on the Fast Pyrolysis of Corn Stover, Prairie Cord Grass, and Switchgrass.

    abstract::Lignocellulosic feedstocks corn stover, prairie cord grass, and switchgrass were subjected to ammonia fiber expansion (AFEX™) pretreatment and densified using extrusion pelleting and ComPAKco densification technique. The effects of AFEX™ pretreatment and densification were studied on the fast pyrolysis product yields....

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Sundaram V,Muthukumarappan K,Gent S

    更新日期:2017-03-01 00:00:00

  • Simulating the degradation of odor precursors in primary and waste-activated sludge during anaerobic digestion.

    abstract::Degradation of known odor precursors in sludge during anaerobic digestion was systematically studied and simulated using the Anaerobic Digestion Model Number 1 (ADM1). The degradation of various protein fractions (particulate, soluble, and bound), volatile fatty acids (VFAs), lipids, and amino acids of primary sludge ...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Aldin S,Tu F,Nakhla G,Ray MB

    更新日期:2011-08-01 00:00:00

  • Enzymatic synthesis of amoxicillin via a one-pot enzymatic hydrolysis and condensation cascade process in the presence of organic co-solvents.

    abstract::A cascade reaction combining the enzymatic hydrolysis of Penicillin G potassium salt (PGK) with the kinetically controlled enzymatic coupling of in situ formed 6-aminopenicillanic acid (6-APA) with p-hydroxyphenylglycine methyl ester (D-HPGM) to give amoxicillin as the final product by using a single enzyme has been d...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Wu Q,Chen CX,Du LL,Lin XF

    更新日期:2010-04-01 00:00:00

  • The constitutive production of pectinase by the CT1 mutant of Penicillium occitainis is modulated by pH.

    abstract::The aim of the present study was to investigate pectinases production by CT1 mutant of Penicillium occitanis on glucose based media. Two main groups of pectinases were followed: lyases (pectin and pectate lyases) and hydrolases (polygalacturonases and polymethylgalacturonases). When cultivated in different liquid medi...

    journal_title:Applied biochemistry and biotechnology

    pub_type: 杂志文章


    authors: Romdhane ZB,Tounsi H,Hadj-Sassi A,Hadj-Taieb N,Gargouri A

    更新日期:2013-01-01 00:00:00