Abstract:
:The use of viruses for treatment of cancer overcomes the bottlenecks of chemotherapy and radiotherapy. Several viruses and their proteins have been evaluated for oncolytic effect. The VP3 protein (apoptin) of chicken anemia virus is one such protein with an inherent ability to lyse cancer and transformed cells while leaving normal cells unharmed. In the present study, the apoptosis inducing potential of VP3 protein of CAV was evaluated in human cervical cancer cell line (HeLa). It was found that in VP3-induced apoptosis, caspase-dependent intrinsic pathway plays an important role with the cleavage of poly (ADP-ribose) polymerase (PARP) and there was no evidence of involvement of death receptor-mediated extrinsic pathway. The results of this study provide intuitive information and strengthen the candidacy of apoptin as a viral oncotherapeutic agent.
journal_name
Appl Biochem Biotechnoljournal_title
Applied biochemistry and biotechnologyauthors
Singh PK,Tiwari AK,Rajmani RS,Kumar GR,Chaturvedi U,Saxena L,Saxena S,Doley J,Sahoo AP,Santra L,Saxena M,Kumar S,Sharma Bdoi
10.1007/s12010-015-1567-5subject
Has Abstractpub_date
2015-05-01 00:00:00pages
196-212issue
1eissn
0273-2289issn
1559-0291journal_volume
176pub_type
杂志文章abstract::The cellulolytic fungus, Trichoderma has oval and mononucleate conidia. When these conidia are incubated in a liquid medium, they begin to swell and their shape becomes spherical followed by an increase in inner space. In such swollen conidia, it is possible to produce a larger autopolyploid nucleus using a mitotic ar...
journal_title:Applied biochemistry and biotechnology
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doi:
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abstract::Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains...
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journal_title:Applied biochemistry and biotechnology
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journal_title:Applied biochemistry and biotechnology
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doi:10.1007/BF02920133
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journal_title:Applied biochemistry and biotechnology
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journal_title:Applied biochemistry and biotechnology
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更新日期:2009-07-01 00:00:00
abstract::The RNA-guided endonuclease clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) derived from CRISPR systems is a simple and efficient genome-editing technology applied to various cell types and organisms. So far, the extensive approach to detect the cleavage activity of custo...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-016-2122-8
更新日期:2016-10-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
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更新日期:2012-02-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
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journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章
doi:10.1007/s12010-013-0309-9
更新日期:2013-08-01 00:00:00
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journal_title:Applied biochemistry and biotechnology
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doi:10.1385/ABAB:131:1:738
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journal_title:Applied biochemistry and biotechnology
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abstract::The use of the insect cell/baculovirus expression system for producing recombinant proteins of bacterial, plant, insect, and mammalian origin has become widespread. The popularity of this eukaryotic expression system is due to many factors, including (1) potentially high protein expression levels, (2) ease and speed o...
journal_title:Applied biochemistry and biotechnology
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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abstract::A bench scale experimental system was developed for the analysis of polycyclic aromatic hydrocarbon (PAH) degradation by mixed microbial cultures in PAH contaminated Manufactured Gas Plant (MGP) soils and on sand. The reactor system was chosen in order to provide a fundamental protocol capable for evaluating the perfo...
journal_title:Applied biochemistry and biotechnology
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doi:10.1007/BF02922585
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abstract::Binding of phenyl derivative of pyranocoumarin (PDP) modulated activity of fungal endopolygalacturonase in silico. Induced fit docking study of PDP with endopolygalacturonase (1HG8) showed a bifurcated hydrogen bond interaction with the protein at Lys 244 with a docking score of -3.6 and glide energy of -37.30 kcal/mo...
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