Preparation of new Calix[4]arene-immobilized biopolymers for enhancing catalytic properties of Candida rugosa lipase by sol-gel encapsulation.

abstract：:In this study, the feasibility of introducing redox property to an amphiphilic phospholipid polymer (PMBN) was investigated. The active ester group in the side chain of the polymer was used to react with pyrroloquinoline quinine (PQQ). Redox peaks that corresponded to PQQ redox potentials were observed after the modif...

journal_title：Applied biochemistry and biotechnology

authors： Yu EH,Himuro Y,Takai M,Ishihara K

更新日期：2010-02-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:Prostate cancer is one of the most common malignancies among men worldwide. The main aim of the present work was to clarify the advantages of a nanoformulation of ayurvedic herbal plants. Specifically, we assessed the improved anticancer activity of Leucas aspera nanoparticles compared with methanolic crude extract in...

journal_title：Applied biochemistry and biotechnology

authors： Mohan A,Nair SV,Lakshmanan VK

更新日期：2017-04-01 00:00:00

abstract：:We studied the inhibitory effects of trifluoroethanol (TFE) on the activity and conformation of tyrosinase. TFE increased the degree of secondary structure of tyrosinase, which directly resulted in enzyme inactivation. A reciprocal study showed that TFE inhibited tyrosinase in a slope-parabolic mixed-type inhibition m...

journal_title：Applied biochemistry and biotechnology

authors： Lü ZR,Shi L,Wang J,Park D,Bhak J,Yang JM,Park YD,Zhou HW,Zou F

更新日期：2010-04-01 00:00:00

abstract：:The effect of four synthetic media containing glucose (initial concentration 30 g l(-1)) on mycelial growth, exopolysaccharides (EPS) and cellular lipids production was examined in 11 mushroom species after 12 and 16 days of culture in static- and shake-flasks. Fatty acid analysis of cellular lipids produced was also ...

journal_title：Applied biochemistry and biotechnology

authors： Diamantopoulou P,Papanikolaou S,Kapoti M,Komaitis M,Aggelis G,Philippoussis A

更新日期：2012-06-01 00:00:00

abstract：:A genetically engineered Escherichia coli cell expressing both organophosphorus hydrolase (OPH) and carboxyl esterase (CaE) B1 intracellularly was constructed and cultivated. The harvested wet cells were vacuum dried, and the storage stability of the dried cell powder was determined in terms of OPH activity. Over a pe...

journal_title：Applied biochemistry and biotechnology

authors： Zheng YZ,Lan WS,Qiao CL,Mulchandani A,Chen W

更新日期：2007-03-01 00:00:00

abstract：:An integrated process of supercritical methanol (SCM) and sodium methoxide catalyst was developed to produce fatty acid methyl esters (FAMEs) via continuous esterification from crude biodiesel. The crude biodiesel with high free fatty acid (FFA) content must be refined to reduce the acid value (AV) for meeting the qua...

journal_title：Applied biochemistry and biotechnology

authors： Zeng D,Li R,Feng M,Fang T

更新日期：2014-10-01 00:00:00

abstract：:Analysis of peroxidase activity by native polyacrylamide gel electrophoresis (PAGE) from a garlic bulb (Allium sativum L) extract showed two major activities (designated POX1 and POX2). The POX2 isoenzyme was purified to homogeneity by ammonium sulfate precipitation, gel filtration, and cation-exchange chromatography....

journal_title：Applied biochemistry and biotechnology

authors： Marzouki SM,Limam F,Smaali MI,Ulber R,Marzouki MN

更新日期：2005-12-01 00:00:00

abstract：:The effects of amino acid composition and peptide molecular mass on ACE-inhibitory and antioxidant activities of protein fragments obtained from tomato waste fermented using Bacillus subtilis were evaluated. The addition of B. subtilis increased the relative amounts of aromatic and positively-charged amino acids which...

journal_title：Applied biochemistry and biotechnology

authors： Moayedi A,Mora L,Aristoy MC,Hashemi M,Safari M,Toldrá F

更新日期：2017-01-01 00:00:00

abstract：:Flavonoids are secondary metabolites synthesized by plants shown to exhibit health benefits such as anti-inflammatory, antioxidant, and anti-tumor effects. Thus, due to the importance of this compound, several enzymes involved in the flavonoid pathway have been cloned and characterized in Escherichia coli. However, th...

journal_title：Applied biochemistry and biotechnology

authors： Zakaria II,Rahman RN,Salleh AB,Basri M

更新日期：2011-09-01 00:00:00

abstract：:With regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (GFOR) and gluconolactonase (GL). Zymomonas mobilis DSM 473 and Rhodotorula rubra DSM 70403 have been selected for the synthesis of GFOR and GL, respectiv...

journal_title：Applied biochemistry and biotechnology

authors： Nidetzky B,Fürlinger M,Gollhofer D,Haug I,Haltrich D,Kulbe KD

更新日期：1997-04-01 00:00:00

abstract：:A bench scale experimental system was developed for the analysis of polycyclic aromatic hydrocarbon (PAH) degradation by mixed microbial cultures in PAH contaminated Manufactured Gas Plant (MGP) soils and on sand. The reactor system was chosen in order to provide a fundamental protocol capable for evaluating the perfo...

journal_title：Applied biochemistry and biotechnology

authors： Webb OF,Phelps TJ,Bienkowski PR,DiGrazia PM,Reed GD,Applegate B,White DC,Sayler GS

更新日期：1991-04-01 00:00:00

abstract：:The wet oxidation pretreatment (water, oxygen, elevated temperature, and pressure) of softwood (Picea abies) was investigated for enhancing enzymatic hydrolysis. The pretreatment was preliminarily optimized. Six different combinations of reaction time, temperature, and pH were applied, and the compositions of solid an...

journal_title：Applied biochemistry and biotechnology

authors： Palonen H,Thomsen AB,Tenkanen M,Schmidt AS,Viikari L

更新日期：2004-04-01 00:00:00

abstract：:Many lab-scale studies have been carried out regarding the effect of feed strategy on the performance of anaerobic sequencing batch reactors (ASBR); however, more detailed pilot-scale studies should be performed to assess the real applicability of this type of operation. Therefore, the objective of this work was to as...

journal_title：Applied biochemistry and biotechnology

authors： de Novaes LF,Borges LO,Rodrigues JA,Ratusznei SM,Zaiat M,Foresti E

更新日期：2010-10-01 00:00:00

abstract：:An animal protein-free medium composed of IPL-41 containing 6 g L(-1) yeastolate ultrafiltrate, 10 g L(-1) glucose, 2 g L(-1) lactose, 5 g L(-1) glutamine, 1% lipid emulsion, and 0.1% Pluronic F-68 was used for producing recombinant proteins in batch mode employing two cell lines, S2AcRVGP2k expressing the G glycoprot...

journal_title：Applied biochemistry and biotechnology

authors： Batista FR,Greco KN,Astray RM,Jorge SA,Augusto EF,Pereira CA,Mendonça RZ,Moraes AM

更新日期：2011-01-01 00:00:00

abstract：:Numerous microcapsule systems have been developed for a wide range of applications, including the sustained release of drugs, cell transplantation for therapy, cell immobilization, and other biotechnological applications. Despite the fact that microcapsule membrane is a dominant factor governing overall microcapsule p...

journal_title：Applied biochemistry and biotechnology

authors： Chen H,Ouyang W,Lawuyi B,Lim T,Prakash S

更新日期：2006-09-01 00:00:00

abstract：:Cross-linked enzyme aggregates (CLEAs) are a versatile and effective method for enzyme immobilization, which is exquisitely simple and amenable to rapid optimization. In this study, nature egg white, which is low cost, easily available, and nontoxic, was used as protein feeder to replace traditional protein feeder (bo...

journal_title：Applied biochemistry and biotechnology

authors： Jiang Y,Wang Q,He Y,Zhou L,Gao J

更新日期：2014-03-01 00:00:00

abstract：:The gene encoding a thermostable β-D-xylosidase (GbtXyl43B) from Geobacillus thermoleovorans IT-08 was cloned in pET30a and expressed in Escherichia coli; additionally, characterization and kinetic analysis of GbtXyl43B were carried out. The gene product was purified to apparent homogeneity showing M r of 72 by sodium...

journal_title：Applied biochemistry and biotechnology

authors： Ratnadewi AA,Fanani M,Kurniasih SD,Sakka M,Wasito EB,Sakka K,Nurachman Z,Puspaningsih NN

更新日期：2013-08-01 00:00:00

abstract：:The purpose of this study was to examine the protective effects of intracellular homogeneous melanin produced by Lachnum YM156 (LIM) against ultraviolet B (UVB) induced damage in mice. The possible structural formula of the LIM was concluded based on elemental analysis, ultraviolet-visible spectroscopy (UV-Vis), Fouri...

journal_title：Applied biochemistry and biotechnology

authors： Li S,Yang L,Li J,Chen T,Ye M

更新日期：2019-06-01 00:00:00

abstract：:Proinflammatory cytokines such as tumor necrosis factor (TNF) alpha are well known to inhibit adipocyte differentiation. TNF-alpha triggers ceramide synthesis through binding of TNF-alpha to its p55 receptor. Therefore, ceramide is implicated in many of the multiple signaling pathways initiated by TNF-alpha. In breast...

journal_title：Applied biochemistry and biotechnology

authors： Xu F,Yang CC,Gomillion C,Burg KJ

更新日期：2010-01-01 00:00:00

abstract：:The vegetative insecticidal proteins (VIPs) of Bacillus thuringiensis (Bt) have a broad-spectrum insecticidal activity against Lepidopteran pests and no cross-resistance with the insecticidal crystal protein Cry protein. So there are great potentials for the control of agricultural pests and the resolution of resistan...

journal_title：Applied biochemistry and biotechnology

authors： Chi B,Li H,Zhang J,Wei P,Gao J,Liu R

更新日期：2019-04-01 00:00:00

abstract：:Acute lymphoblastic leukaemia (ALL) is one of the leading types of malignant disorder seen in children. Viral infections, genetic factors and exposure to chemical carcinogens are some of the factors responsible for causing ALL. Treatment strategies followed for curing ALL include chemotherapy or radiation therapy, whe...

journal_title：Applied biochemistry and biotechnology

authors： Ramya LN,Doble M,Rekha VP,Pulicherla KK

更新日期：2012-08-01 00:00:00

abstract：:Fructose-1,6-bisphosphate (F-1,6-P2) is an allosteric activator of two key enzymes of glycolysis: phosphofructokinase and pyruvate kinase. Regulation of glycolysis in a wild-type Saccharomyces cerevisiae and a recombinant Escherichia coli by a dead-end structural analog of F-1,6-P2 was studied. 2,5-Anhydromannitol (2,...

journal_title：Applied biochemistry and biotechnology

authors： Nghiem NP,Cofer TM

更新日期：2007-05-01 00:00:00

abstract：:The 1,3-dihydroxyacetone (DHA)-overproducing mutant of Gluconobacter oxydans was screened via UV mutagenesis to enhance the DHA production, and the DHA fermentation condition was optimized using the dissolved oxygen (DO) control strategy. The stable mutant G. oxydans ZJB11001 exhibits high DHA productivity and can tol...

journal_title：Applied biochemistry and biotechnology

authors： Hu ZC,Zheng YG

更新日期：2011-11-01 00:00:00

abstract：:Neoadjuvant chemotherapy (NACT) is known to be beneficial for patients with locally advanced breast cancer. However, there is still no unified standard on the evaluation of NACT. To identify the potential markers related to NACT sensitivity of breast cancer, in the present study, we examined the protein spectrum of br...

journal_title：Applied biochemistry and biotechnology

authors： Zhang K,Yuan K,Wu H,Li Q,Wang Y,Chen S,Zhang L,Gu H,Fu R

更新日期：2012-02-01 00:00:00

abstract：:Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupriavidus pinatubonensis (designated ALS1280), Pigmentiphaga sp. (ALS1172), Pseudomonas sp. BWDY (ALS1279), Ps...

journal_title：Applied biochemistry and biotechnology

authors： Lee SA,Wrona LJ,Cahoon AB,Crigler J,Eiteman MA,Altman E

更新日期：2016-01-01 00:00:00

abstract：:Since the two reports published in 1986 by the laboratories of R. Lerner and P. G. Schultz, it has been clearly established that antibodies may be induced to act as catalysts in numerous chemical reactions. In all cases, catalytic antibodies were elicited using a substrate-based approach. In the present article, we pr...

journal_title：Applied biochemistry and biotechnology

authors： Friboulet A,Izadyar L,Avalle B,Roseto A,Thomas D

更新日期：1994-05-01 00:00:00

abstract：:L-Ribose is an important precursor for antiviral agents, and thus its high-level production is urgently demanded. For this aim, immobilized recombinant Escherichia coli cells expressing the L-arabinose isomerase and variant mannose-6-phosphate isomerase genes from Geobacillus thermodenitrificans were developed. The im...

journal_title：Applied biochemistry and biotechnology

authors： Kim KR,Seo ES,Oh DK

更新日期：2014-01-01 00:00:00

abstract：:This study proposes an innovative strategy of lignocellulose biodegradation by Inonotus obliquus under solid-state fermentation in extracting Eucommia ulmoides trans-1,4-polyisoprene (EUG) and producing reducing sugars efficiently. EUG and sugars were obtained through the white rot fungal pretreatment of E. ulmoides l...

journal_title：Applied biochemistry and biotechnology

authors： Qian S,Zhang C,Zhu Z,Huang P,Xu X

更新日期：2020-11-01 00:00:00

abstract：:An efficient metabolic control approach was developed to improve the industrial anaerobic fermentation of adenosylcobalamin (ado-cbl) by Propionibacterium freudenreichii. The effects of 5,6-dimethylbenzimidazole (DMB) on cell growth and ado-cbl biosynthesis were investigated. Subsequently, the results obtained from th...

journal_title：Applied biochemistry and biotechnology

authors： Wang P,Wang Y,Su Z

更新日期：2012-05-01 00:00:00